FastQCFastQC Report
Mon 15 Aug 2016
SRR2921707_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921707_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989763
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.044.6181221
GTATCAA28400.039.9399641
TCAACGC33100.033.2667164
TATCAAC35200.031.82142
CAACGCA34750.031.6863485
ATCAACG35950.030.7614613
GTACATG47600.029.911791
AACGCAG37550.029.307376
TACATGG50300.027.5527382
ACATGGG51200.026.2341163
ACGCAGA43950.024.5042957
GTGGTAT10000.024.2046221
CATGGGG43750.024.0837924
CGCAGAG45750.023.4376668
TAAGGTG10100.020.676185
TAAGGTA5100.020.4739884
GCAGAGT50200.020.227399
TAAGGCG5750.019.8099045
ATGGGGG24450.019.6056615
GTATATA4250.018.9840151
Produced by FastQC (version 0.11.5)