##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921707_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1989763
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.710995731652464	31.0	31.0	34.0	27.0	34.0
2	30.77684025685471	33.0	31.0	34.0	27.0	34.0
3	30.83746808036937	33.0	31.0	34.0	27.0	34.0
4	34.303504487720396	37.0	35.0	37.0	30.0	37.0
5	34.30329340730529	37.0	35.0	37.0	30.0	37.0
6	34.3433901424441	37.0	35.0	37.0	30.0	37.0
7	34.258189040604336	37.0	35.0	37.0	30.0	37.0
8	34.23361023398264	37.0	35.0	37.0	30.0	37.0
9	35.82621699167187	39.0	37.0	39.0	30.0	39.0
10-11	35.73256387821062	39.0	37.0	39.0	30.0	39.0
12-13	35.63021776965397	39.0	37.0	39.0	30.0	39.0
14-15	36.81441558617785	40.0	37.0	41.0	30.0	41.0
16-17	36.75020417004437	40.0	37.0	41.0	30.0	41.0
18-19	36.71721757817389	40.0	37.0	41.0	30.0	41.0
20-21	36.637678708469295	40.0	37.0	41.0	30.0	41.0
22-23	36.53308559863662	40.0	37.0	41.0	29.5	41.0
24-25	36.41970877938729	40.0	37.0	41.0	29.0	41.0
26-27	36.27112022889158	40.0	36.5	41.0	28.5	41.0
28-29	36.13247683266801	40.0	36.0	41.0	28.0	41.0
30-31	35.97544556814053	40.0	36.0	41.0	27.0	41.0
32-33	35.7840891101101	40.0	36.0	41.0	27.0	41.0
34-35	35.606773017691054	39.5	35.0	41.0	26.0	41.0
36-37	35.565011762707414	39.5	35.0	41.0	26.5	41.0
38-39	35.73232415116775	40.0	35.5	41.0	26.5	41.0
40-41	35.7784947252512	40.0	36.0	41.0	27.0	41.0
42-43	35.59679368849456	40.0	35.0	41.0	26.0	41.0
44-45	35.45410433302861	40.0	35.0	41.0	25.0	41.0
46-47	35.27233796185777	40.0	35.0	41.0	24.0	41.0
48-49	35.08815195578569	39.5	35.0	41.0	24.0	41.0
50-51	33.356238908854976	37.0	32.5	39.5	20.5	40.0
52-53	33.4720728046506	37.5	33.5	39.5	21.0	40.0
54-55	34.37989122322608	39.0	34.0	40.5	22.0	41.0
56-57	34.37927406429811	39.0	34.0	41.0	22.0	41.0
58-59	34.17501154660128	38.0	34.0	41.0	21.5	41.0
60-61	33.90527565343209	38.0	34.0	40.0	20.0	41.0
62-63	33.60128492689833	37.0	34.0	40.0	19.5	41.0
64-65	33.29001469019175	37.0	34.0	40.0	18.0	41.0
66-67	32.92441009306134	36.0	33.0	39.0	15.5	41.0
68-69	32.54235956744597	35.5	33.0	39.0	12.0	41.0
70-71	32.110230715919435	35.0	33.0	38.5	7.5	40.5
72-73	31.72569044655067	35.0	32.5	37.0	4.0	40.0
74-75	31.30575500700335	35.0	32.0	37.0	2.0	39.0
76-77	30.908591374952696	35.0	32.0	36.5	2.0	39.0
78-79	30.540525429410437	35.0	31.5	36.0	2.0	38.0
80-81	30.20330989168057	35.0	31.0	35.5	2.0	37.0
82-83	29.877336396344692	35.0	31.0	35.0	2.0	37.0
84-85	29.59625292057396	35.0	31.0	35.0	2.0	36.0
86-87	29.338249329191466	35.0	31.0	35.0	2.0	36.0
88-89	29.083097584988764	35.0	30.5	35.0	2.0	36.0
90-91	28.814696775445114	35.0	30.0	35.0	2.0	35.0
92-93	28.584878450348107	34.0	30.0	35.0	2.0	35.0
94-95	28.31269553208096	34.0	29.5	35.0	2.0	35.0
96-97	28.024231529081604	34.0	29.0	35.0	2.0	35.0
98-99	27.70057388744288	34.0	29.0	35.0	2.0	35.0
100-101	26.577016207457874	33.0	26.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55459.0
3	10313.0
4	7210.0
5	5772.0
6	5401.0
7	5556.0
8	5878.0
9	6364.0
10	6926.0
11	7320.0
12	7425.0
13	7616.0
14	8281.0
15	9190.0
16	9470.0
17	9903.0
18	9377.0
19	8821.0
20	9039.0
21	9526.0
22	10481.0
23	11667.0
24	13733.0
25	16339.0
26	19202.0
27	22409.0
28	25797.0
29	30324.0
30	36089.0
31	43887.0
32	54027.0
33	69230.0
34	96412.0
35	142449.0
36	240907.0
37	433265.0
38	443584.0
39	75105.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32717479084053	17.79990083844813	12.473235935100474	25.399688435610862
2	16.931360098577912	19.665136864870302	37.81599627818083	25.58750675837095
3	19.572387446425214	22.74603664761897	29.28801986780946	28.393556038146357
4	14.054938677057988	15.20293955820122	35.1184866063211	35.623635158419695
5	14.887753321333935	36.29551511164822	33.973686817710224	14.843044749307621
6	34.76299761052455	34.01876195617318	16.36328269897133	14.854957734330942
7	29.401501987688345	31.1135427698559	20.71000861021655	18.774946632239203
8	26.74809453460802	33.82029537415383	20.079883020535245	19.351727070702907
9	26.751405401466087	14.943871896745401	19.384412797257802	38.92030990453071
10-11	25.981253779175994	24.572020099310336	27.753589934461615	21.69313618705206
12-13	26.30484548457862	22.336999249993465	27.201686408795787	24.15646885663213
14-15	23.613932171212987	24.0864541520195	24.43461765865195	27.864996018115562
16-17	23.827927648495674	26.877846888725454	25.55145626480156	23.742769197977314
18-19	23.826782845757783	26.201338780401013	26.825266941834723	23.14661143200648
20-21	24.24959949652428	25.62561231665687	26.60763125631167	23.51715693050718
22-23	24.29484933206572	25.34937655948112	26.29993418945694	24.05583991899622
24-25	24.132908745017755	25.525121363616254	26.29822368712118	24.043746204244812
26-27	23.825451401455744	25.661745485985442	26.379865886516267	24.132937226042547
28-29	23.992192218002685	25.871030861949677	26.284716646222385	23.852060273825256
30-31	24.228426126766635	25.642411727053332	26.02634209132853	24.102820054851502
32-33	23.89348106018341	25.754868522500303	25.921156724557175	24.43049369275911
34-35	24.362515395098345	25.72874320460799	25.92872556354039	23.980015836753278
36-37	24.397242570240554	25.541707092569098	25.971961825383723	24.089088511806626
38-39	24.155402985839764	25.533681980347207	26.10226258693873	24.2086524468743
40-41	24.35704684696955	25.442085654715967	26.163308907249146	24.037558591065338
42-43	24.253881624036786	25.408992688242677	25.898819222599673	24.43830646512086
44-45	24.043367041823757	25.52848451793988	26.04301301076723	24.38513542946913
46-47	24.235941905194547	25.467746988036755	25.944055697523442	24.35225540924525
48-49	23.9812836278333	25.523697037539772	25.922779854716428	24.5722394799105
50-51	23.99423554346975	25.658227558040537	26.030177529245957	24.31735936924376
52-53	24.266342779320098	25.431964518117677	25.958088386392298	24.343604316169923
54-55	23.762650745005804	25.29610109012342	26.231985515384864	24.70926264948591
56-57	23.73613876321278	25.50240923745003	26.13908051592484	24.622371483412348
58-59	23.891583682145356	25.25567795457636	26.16606474555611	24.686673617722168
60-61	23.719789583019555	25.260439622990315	26.365264776715303	24.654506017274823
62-63	23.828476570035047	25.26296730366409	26.35482618345989	24.553729942840967
64-65	23.713652560405936	25.089031868145707	26.20823207967666	24.989083491771698
66-67	23.661865784301504	25.159620210291994	26.129844163333242	25.048669842073256
68-69	23.80083417603713	25.084530577054014	26.130272270546197	24.98436297636266
70-71	23.671961865704112	24.89550937041752	26.178201089321107	25.254327674557263
72-73	23.700032433717368	24.9673396495144	26.1373205317518	25.19530738501643
74-75	23.693842965529573	25.143041324728106	26.1071255001599	25.055990209582426
76-77	23.744368005487086	24.778635610773346	26.29201617723856	25.184980206501013
78-79	23.770494486827847	24.873053365310398	26.163032142688948	25.19342000517281
80-81	23.842342124511127	24.926202868063825	26.10088697604036	25.130568031384687
82-83	23.70591231047526	24.855792698131086	26.221499191754027	25.216795799639623
84-85	23.721701952756664	24.86286679668926	26.25485066206752	25.160580588486553
86-87	23.786864255102294	24.962157404540807	26.080620535436516	25.17035780492038
88-89	23.70794415887562	24.64191248017342	26.16141513618598	25.488728224764984
90-91	23.741796791163658	24.640635721605282	26.217672077199083	25.39989541003197
92-93	23.867880708959323	24.43012422203795	26.32365639392361	25.37833867507912
94-95	23.724215342087145	24.283279145741236	26.39232555579542	25.600179956376195
96-97	23.85522749842174	24.074193501254292	26.402306952447695	25.66827204787627
98-99	23.829707054580336	24.050901074054277	26.283547507719497	25.835844363645887
100-101	24.116934285239246	23.876883700542624	26.351503181696806	25.654678832521327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	64.0
2	77.5
3	118.0
4	116.5
5	140.0
6	204.5
7	362.5
8	567.0
9	642.0
10	598.5
11	548.0
12	545.0
13	607.0
14	694.0
15	774.5
16	845.5
17	904.5
18	992.5
19	1105.0
20	1214.5
21	1322.5
22	1415.5
23	1537.5
24	1707.5
25	1927.5
26	2241.5
27	2748.5
28	3485.0
29	4598.5
30	6537.0
31	9361.5
32	13366.0
33	17019.5
34	20387.0
35	24647.0
36	29657.0
37	34801.0
38	39938.0
39	44951.0
40	49318.5
41	53720.0
42	58665.0
43	64181.0
44	69331.0
45	75313.5
46	79619.0
47	82363.5
48	86229.5
49	88229.5
50	88116.5
51	87930.0
52	87945.5
53	87940.0
54	86978.0
55	82881.5
56	76352.5
57	68822.5
58	62554.0
59	55908.5
60	46717.0
61	38685.5
62	32306.5
63	25338.0
64	18623.0
65	13924.5
66	10880.0
67	8670.0
68	6837.5
69	5411.5
70	4273.5
71	3259.5
72	2371.5
73	1758.0
74	1419.5
75	1152.0
76	836.5
77	567.0
78	389.0
79	254.5
80	158.5
81	110.5
82	91.5
83	80.0
84	73.5
85	65.5
86	47.0
87	38.5
88	33.5
89	23.0
90	30.0
91	33.0
92	22.0
93	14.0
94	9.0
95	10.5
96	11.0
97	8.0
98	7.0
99	6.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05473013620215071
2	0.0747827756370985
3	0.07141554044376139
4	0.07221965631082697
5	0.06684213144982594
6	0.010302734546777681
7	0.013368426289965188
8	0.01779106355882585
9	0.005930354519608616
10-11	0.012162252489366823
12-13	0.022464987036144506
14-15	0.03794421747715683
16-17	0.026636338096547175
18-19	0.009599133163095302
20-21	0.019700838743106592
22-23	0.036185214017950884
24-25	0.035129811942427314
26-27	0.020253668401714175
28-29	0.010528892134389874
30-31	0.010528892134389874
32-33	0.007287300045281775
34-35	0.0043975086480148645
36-37	5.779582794533821E-4
38-39	0.0035933927809492887
40-41	9.297589712945713E-4
42-43	0.23482696180399373
44-45	0.20411978713042708
46-47	0.2101255275125731
48-49	0.21789027135392505
50-51	0.20839165267421295
52-53	0.21605588203218173
54-55	0.21148247303824627
56-57	0.21510099444004135
58-59	0.22379549725268788
60-61	0.22927353659707214
62-63	0.21359327718929338
64-65	0.2129901902889942
66-67	0.2106029713086433
68-69	0.20560237576032922
70-71	0.1979632750232063
72-73	0.20982398406242353
74-75	0.20846703853675033
76-77	0.2015315391833098
78-79	0.22020210447173857
80-81	0.23073099660612847
82-83	0.23118331178135285
84-85	0.22449909863637027
86-87	0.2460845839429118
88-89	0.23814393975563922
90-91	0.24470250979639285
92-93	0.23967678562723302
94-95	0.24311940668310747
96-97	0.25028106362416025
98-99	0.24394865117101888
100-101	0.2499292629323191
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1989763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.78441111725277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.616369602853	49.9866688080867
2	11.429816396375509	14.352285832495133
3	3.748760915410708	7.060912394803041
4	1.683402858492887	4.227658285743037
5	0.9121314846948361	2.863381906403537
6	0.5812735695869077	2.1896951258722472
7	0.3877607934718521	1.704173315074223
8	0.296629646769609	1.4898974153878883
9	0.2224242961659255	1.256830060765235
>10	1.075495635737207	11.33039339039822
>50	0.03404960813442066	1.4423384259909635
>100	0.01044035602090776	1.2927012786585508
>500	9.632241908267076E-4	0.400448361437873
>1k	4.8161209541334153E-4	0.4026153988833504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.025724169159845E-5
5	0.0	5.025724169159845E-5	0.0	0.0	5.025724169159845E-5
6	0.0	5.025724169159845E-5	0.0	0.0	5.025724169159845E-5
7	0.0	5.025724169159845E-5	0.0	0.0	5.025724169159845E-5
8	0.0	5.025724169159845E-5	0.0	0.0	5.025724169159845E-5
9	0.0	5.025724169159845E-5	0.0	0.0	1.5077172507479533E-4
10-11	0.0	5.025724169159845E-5	0.0	0.0	1.5077172507479533E-4
12-13	0.0	5.025724169159845E-5	0.0	0.0	2.7641482930379144E-4
14-15	0.0	5.025724169159845E-5	0.0	0.0	4.020579335327876E-4
16-17	0.0	5.025724169159845E-5	0.0	0.0	5.528296586075829E-4
18-19	0.0	1.5077172507479533E-4	0.0	0.0	6.533441419907798E-4
20-21	0.0	1.5077172507479533E-4	0.0	0.0	7.287300045281774E-4
22-23	0.0	1.5077172507479533E-4	0.0	1.256431042289961E-4	8.795017296029729E-4
24-25	0.0	1.5077172507479533E-4	0.0	3.7692931268698836E-4	9.548875921403704E-4
26-27	0.0	1.5077172507479533E-4	0.0	0.0012815596631357605	0.0011056593172151657
28-29	0.0	1.5077172507479533E-4	0.0	0.006508312799061999	0.0011810451797525635
30-31	2.5128620845799223E-5	1.5077172507479533E-4	0.0	0.02839534155575312	0.0012313024214441618
32-33	5.025724169159845E-5	1.5077172507479533E-4	0.0	0.08005978601471633	0.0013066882839815597
34-35	5.025724169159845E-5	2.010289667663938E-4	0.0	0.16682891379526105	0.001356945525673158
36-37	5.025724169159845E-5	2.7641482930379144E-4	0.0	0.2963418256345102	0.001356945525673158
38-39	5.025724169159845E-5	3.0154345014959065E-4	0.0	0.4868167716456684	0.0013820741465189572
40-41	5.025724169159845E-5	3.0154345014959065E-4	0.0	0.6978218008878444	0.0014072027673647566
42-43	5.025724169159845E-5	3.0154345014959065E-4	0.0	0.9231250153912802	0.0014825886299021543
44-45	5.025724169159845E-5	3.0154345014959065E-4	0.0	1.208259476128564	0.0015077172507479534
46-47	5.025724169159845E-5	3.0154345014959065E-4	0.0	1.5437516930408295	0.0015077172507479534
48-49	5.025724169159845E-5	3.0154345014959065E-4	0.0	1.8726350826706497	0.0017087462175143472
50-51	5.025724169159845E-5	3.0154345014959065E-4	0.0	2.1996086971161892	0.001809260700897544
52-53	5.025724169159845E-5	3.0154345014959065E-4	0.0	2.5308793057263603	0.0019600324259723394
54-55	5.025724169159845E-5	3.0154345014959065E-4	0.0	2.9233632347168985	0.0020605469093555365
56-57	5.025724169159845E-5	3.0154345014959065E-4	0.0	3.385227285862688	0.0020856755302013355
58-59	5.025724169159845E-5	3.5180069184118914E-4	0.0	3.8535493925658484	0.0021359327718929342
60-61	5.025724169159845E-5	3.5180069184118914E-4	0.0	4.327977754134538	0.002161061392738733
62-63	5.025724169159845E-5	3.5180069184118914E-4	0.0	4.800345568793871	0.002161061392738733
64-65	5.025724169159845E-5	3.5180069184118914E-4	0.0	5.330157410706702	0.002161061392738733
66-67	5.025724169159845E-5	3.5180069184118914E-4	0.0	5.956639057013323	0.0022615758761219303
68-69	5.025724169159845E-5	3.5180069184118914E-4	0.0	6.619230531475356	0.002362090359505127
70-71	5.025724169159845E-5	3.5180069184118914E-4	0.0	7.277952198327137	0.002462604842888324
72-73	5.025724169159845E-5	3.5180069184118914E-4	0.0	7.961199399124418	0.0027892769138837138
74-75	5.025724169159845E-5	3.5180069184118914E-4	0.0	8.687466798809709	0.0029149200181127098
76-77	5.025724169159845E-5	3.5180069184118914E-4	0.0	9.502161815251362	0.0029149200181127098
78-79	5.025724169159845E-5	3.5180069184118914E-4	0.0	10.380583014157967	0.0029149200181127098
80-81	5.025724169159845E-5	3.5180069184118914E-4	0.0	11.26792487346483	0.0029651772598043085
82-83	5.025724169159845E-5	4.020579335327876E-4	0.0	12.166574612152303	0.003040563122341706
84-85	5.025724169159845E-5	4.020579335327876E-4	0.0	13.11729085323227	0.003065691743187505
86-87	5.025724169159845E-5	4.020579335327876E-4	0.0	14.13469845403699	0.003065691743187505
88-89	5.025724169159845E-5	4.020579335327876E-4	0.0	15.232115583614732	0.0030908203640333046
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1670	0.0	44.618122	1
GTATCAA	2840	0.0	39.939964	1
TCAACGC	3310	0.0	33.266716	4
TATCAAC	3520	0.0	31.8214	2
CAACGCA	3475	0.0	31.686348	5
ATCAACG	3595	0.0	30.761461	3
GTACATG	4760	0.0	29.91179	1
AACGCAG	3755	0.0	29.30737	6
TACATGG	5030	0.0	27.552738	2
ACATGGG	5120	0.0	26.234116	3
ACGCAGA	4395	0.0	24.504295	7
GTGGTAT	1000	0.0	24.204622	1
CATGGGG	4375	0.0	24.083792	4
CGCAGAG	4575	0.0	23.437666	8
TAAGGTG	1010	0.0	20.67618	5
TAAGGTA	510	0.0	20.473988	4
GCAGAGT	5020	0.0	20.22739	9
TAAGGCG	575	0.0	19.809904	5
ATGGGGG	2445	0.0	19.605661	5
GTATATA	425	0.0	18.984015	1
>>END_MODULE