##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921707_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1989763
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26666995013979	33.0	31.0	34.0	28.0	34.0
2	31.405867935025427	34.0	31.0	34.0	28.0	34.0
3	31.471932586946284	34.0	31.0	34.0	28.0	34.0
4	34.948263185113	37.0	35.0	37.0	32.0	37.0
5	34.663476001915804	37.0	35.0	37.0	32.0	37.0
6	34.63561891541857	37.0	35.0	37.0	32.0	37.0
7	34.512516314757086	37.0	35.0	37.0	31.0	37.0
8	34.45053204828917	37.0	35.0	37.0	31.0	37.0
9	36.03022721801541	39.0	37.0	39.0	31.0	39.0
10-11	35.92039730359847	39.0	37.0	39.0	31.0	39.0
12-13	35.83650565419097	39.0	37.0	39.0	30.0	39.0
14-15	37.05792926092203	40.0	38.0	41.0	31.0	41.0
16-17	37.010578144231246	40.0	38.0	41.0	31.0	41.0
18-19	36.96361626987736	40.0	37.5	41.0	30.5	41.0
20-21	36.897546089659926	40.0	37.0	41.0	30.0	41.0
22-23	36.79796940640669	40.0	37.0	41.0	30.0	41.0
24-25	36.706620587477005	40.0	37.0	41.0	30.0	41.0
26-27	36.56605133375181	40.0	37.0	41.0	30.0	41.0
28-29	36.43201300858444	40.0	37.0	41.0	29.5	41.0
30-31	36.2606539070231	40.0	36.5	41.0	29.0	41.0
32-33	36.193984157912276	40.0	36.0	41.0	28.5	41.0
34-35	36.31741971279997	40.0	37.0	41.0	29.5	41.0
36-37	36.294301130335626	40.0	37.0	41.0	29.0	41.0
38-39	36.1678491358016	40.0	36.0	41.0	28.0	41.0
40-41	36.066646128207225	40.0	36.0	41.0	28.0	41.0
42-43	35.919387635612885	40.0	36.0	41.0	27.5	41.0
44-45	35.76384071871877	40.0	35.5	41.0	27.0	41.0
46-47	35.58212510736203	40.0	35.0	41.0	26.0	41.0
48-49	35.41707756149853	40.0	35.0	41.0	26.0	41.0
50-51	35.239387052628885	39.0	35.0	41.0	25.5	41.0
52-53	35.04645829679213	39.0	35.0	41.0	24.5	41.0
54-55	34.813150611404474	39.0	35.0	41.0	24.0	41.0
56-57	34.565237417722614	38.5	34.5	41.0	23.0	41.0
58-59	34.30887045341581	38.0	34.0	40.0	23.0	41.0
60-61	34.0181973933579	37.5	34.0	40.0	22.5	41.0
62-63	33.69988636837654	37.0	34.0	40.0	22.0	41.0
64-65	33.34597989810847	36.5	34.0	39.5	20.5	41.0
66-67	32.98316407531952	36.0	33.0	39.0	20.0	41.0
68-69	32.61690035446432	35.5	33.0	39.0	18.5	40.5
70-71	32.21615689908798	35.0	33.0	38.0	16.5	40.0
72-73	31.8560763266781	35.0	33.0	37.0	13.5	39.5
74-75	31.459279321205592	35.0	32.0	37.0	9.5	39.0
76-77	28.778643235400395	32.0	28.0	34.5	4.5	36.0
78-79	30.343068496097274	34.5	31.0	35.0	5.5	37.0
80-81	30.528367448786614	35.0	31.5	35.0	2.0	37.0
82-83	30.36501834640608	35.0	32.0	35.0	2.0	36.5
84-85	30.172577588386154	35.0	31.0	35.0	2.0	36.0
86-87	29.965315718505167	35.0	31.0	35.0	2.0	36.0
88-89	29.69477998133446	34.5	31.0	35.0	2.0	35.5
90-91	29.497501209943096	34.0	31.0	35.0	2.0	35.0
92-93	29.340561664881697	34.0	31.0	35.0	2.0	35.0
94-95	29.22984571529373	34.0	31.0	35.0	2.0	35.0
96-97	29.012988230256568	34.0	31.0	35.0	2.0	35.0
98-99	28.792754212436357	34.0	30.5	35.0	2.0	35.0
100-101	27.646315164167792	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41688.0
3	14464.0
4	8005.0
5	5874.0
6	5153.0
7	5069.0
8	5197.0
9	5648.0
10	6199.0
11	6594.0
12	6990.0
13	7519.0
14	7976.0
15	8827.0
16	9455.0
17	10475.0
18	9881.0
19	8705.0
20	8456.0
21	8249.0
22	8578.0
23	9562.0
24	11188.0
25	13053.0
26	15723.0
27	18487.0
28	22134.0
29	27021.0
30	32473.0
31	40571.0
32	51082.0
33	67149.0
34	94529.0
35	143055.0
36	253580.0
37	473797.0
38	464712.0
39	52644.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.35432671299494	19.250112263164993	12.998262186342757	26.397298837497306
2	15.993026298133293	20.87235602667858	36.38049996959434	26.75411770559379
3	18.347651349438674	23.7489746517837	28.943217824170876	28.960156174606748
4	13.62948942250618	16.22984757613026	34.09274701216144	36.04791598920212
5	14.696977237412865	36.5842194610795	33.981660636825616	14.73714266468202
6	34.843154441210054	34.54431962559706	16.032342109072196	14.580183824120684
7	29.283990103343964	31.332073216759987	20.63708089857938	18.74685578131667
8	26.77288702222325	34.17773875582167	19.801604512698244	19.247769709256833
9	26.609953044659086	14.874635823462393	19.157809246628872	39.35760188524965
10-11	25.96140091086107	24.81793463572471	27.69711543900713	21.52354901440709
12-13	26.22253683366202	22.4508683476768	27.226227738476478	24.100367080184707
14-15	23.493700685694698	24.278809304700648	24.370005744697213	27.85748426490744
16-17	23.81190624210019	27.106846393263922	25.432626900791703	23.648620463844185
18-19	23.821494777318282	26.354747329354744	26.729671722533837	23.094086170793137
20-21	24.3121410058796	25.659639524545913	26.54585148668609	23.482367982888398
22-23	24.236529677152504	25.509294322992236	26.24817629034212	24.005999709513144
24-25	24.071928363129675	25.625610156011476	26.225758666571174	24.07670281428767
26-27	23.77535391508551	25.79728427419608	26.3847324292641	24.042629381454308
28-29	23.946934865515253	26.010042881525962	26.226661935559097	23.81636031739969
30-31	24.190725840400816	25.747586333425904	25.953949641924094	24.107738184249182
32-33	23.878175405581867	25.84324387084711	25.8756632784616	24.40291744510942
34-35	24.257592827755506	25.81610442718824	25.949802431041864	23.97650031401439
36-37	24.386026413761993	25.590590458527927	25.997187993561788	24.02619513414829
38-39	24.114661925732342	25.666279266997943	26.069518347038905	24.149540460230806
40-41	24.27629938925589	25.532164583274696	26.117000317635185	24.074535709834226
42-43	24.265956505742874	25.509399898270807	25.779530115661668	24.445113480324647
44-45	24.009769616382478	25.60229549294897	25.992115148584816	24.395819742083734
46-47	24.15714200633411	25.55687317230934	25.86518544094635	24.420799380410195
48-49	23.860868000564935	25.700753967994682	25.87807541891439	24.56030261252599
50-51	23.94885097894649	25.75453626410409	25.85826968729952	24.438343069649907
52-53	24.185143154669767	25.556757278352503	25.843905925208166	24.41419364176956
54-55	23.685586038823153	25.467824368531964	26.039848438625334	24.806741154019548
56-57	23.680086488920743	25.542453102830244	25.997188406423543	24.780272001825477
58-59	23.723514891174368	25.390142183811044	25.997892902973508	24.888450022041077
60-61	23.59182670306548	25.39090899267224	26.12056892113616	24.896695383126122
62-63	23.6987596271467	25.419085470388364	26.05132350254089	24.830831399924048
64-65	23.480107783807945	25.26489059852995	25.936038118001605	25.31896349966051
66-67	23.450816556002323	25.363925100925478	25.844968007579215	25.34029033549298
68-69	23.53201901956185	25.33119937731123	25.830521956893985	25.30625964623293
70-71	23.393194540625707	25.217721693413857	25.780124349173445	25.608959416786988
72-73	23.346712831297168	25.26138535115903	25.688761774720692	25.703140042823108
74-75	23.28661383598224	25.327435292130495	25.686943005598096	25.69900786628917
76-77	23.244052858554333	25.050667154260108	25.885847222568525	25.819432764617034
78-79	23.199385738541007	25.08706270904293	25.810977785836926	25.902573766579135
80-81	23.189812827655953	25.08974903461797	25.75124034335088	25.969197794375198
82-83	22.999402031885097	25.078993875288337	25.835439878566476	26.086164214260094
84-85	22.945111028704677	24.971823284287538	25.86264243917462	26.220423247833164
86-87	22.924943724389315	25.143354448840995	25.725497729864998	26.20620409690469
88-89	22.770983680304845	24.860650645617778	25.78443788346747	26.583927790609906
90-91	22.59126382551342	24.751024193782126	25.891946453347042	26.76576552735741
92-93	22.64856298871596	24.591496879735363	25.9800718411359	26.77986829041278
94-95	22.442286524223828	24.41062641676342	26.08357584804757	27.063511210965185
96-97	22.422834250873663	24.273656141655998	26.122341782411624	27.181167825058715
98-99	22.252217683115756	24.287603615638282	26.015161235444435	27.44501746580153
100-101	22.10804318108717	24.215005829848828	26.05482570762303	27.62212528144098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	48.5
2	70.5
3	104.5
4	98.5
5	110.0
6	173.0
7	329.5
8	501.0
9	535.0
10	463.0
11	408.5
12	410.5
13	440.5
14	461.0
15	470.5
16	531.5
17	595.0
18	641.0
19	719.5
20	821.0
21	922.5
22	1035.5
23	1211.5
24	1383.5
25	1631.0
26	1998.5
27	2537.5
28	3291.5
29	4572.0
30	6653.0
31	9546.5
32	13701.0
33	17384.5
34	20496.0
35	24466.5
36	29532.0
37	34738.0
38	39732.0
39	44810.5
40	49310.5
41	53474.0
42	58400.5
43	64018.0
44	69485.5
45	75390.5
46	79446.0
47	82508.5
48	86417.0
49	88891.5
50	89323.0
51	88922.5
52	88971.5
53	89473.0
54	88280.5
55	83886.5
56	77289.5
57	69253.0
58	62770.5
59	56113.0
60	46797.0
61	38669.0
62	32109.5
63	25072.0
64	18395.0
65	13593.0
66	10470.5
67	8417.5
68	6769.0
69	5359.0
70	4078.5
71	2947.0
72	2119.5
73	1607.0
74	1244.5
75	954.5
76	721.5
77	478.0
78	299.5
79	168.0
80	95.0
81	59.5
82	35.0
83	23.0
84	15.0
85	10.5
86	8.5
87	8.0
88	4.5
89	1.0
90	1.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16881407484207916
2	1.005144833831969E-4
3	0.009800162129861697
4	0.005729325552842223
5	0.02462604842888324
6	0.0017590034592059457
7	0.0
8	0.0
9	0.0
10-11	0.04952851168707027
12-13	3.5180069184118914E-4
14-15	0.005126238652543041
16-17	0.0
18-19	0.0014323313882105557
20-21	1.005144833831969E-4
22-23	0.0
24-25	2.7641482930379144E-4
26-27	0.0027892769138837138
28-29	0.004774437960701853
30-31	0.015604873545241319
32-33	0.010755049722002068
34-35	0.010277605925931882
36-37	0.004045707956173675
38-39	2.5128620845799223E-5
40-41	0.002965177259804308
42-43	0.00839295936249694
44-45	0.006282155211449806
46-47	0.0029903058806501075
48-49	0.007840129703889358
50-51	0.0023872189803509263
52-53	0.001356945525673158
54-55	0.003719035885178285
56-57	0.007237042803590177
58-59	0.01487614354071314
60-61	0.006910370732594786
62-63	0.020178282539176775
64-65	0.03927603438198419
66-67	0.05865020105409539
68-69	0.0486741385783131
70-71	0.06684213144982594
72-73	0.03266720709953899
74-75	0.0258824794711732
76-77	0.226283230716422
78-79	0.08465832362949759
80-81	0.06540980006161538
82-83	0.02646043775062658
84-85	0.049025939270154285
86-87	0.06709341765828393
88-89	0.2920197028490328
90-91	0.29578899597590264
92-93	0.053750119989164534
94-95	0.0096996476464785
96-97	0.014851014919867341
98-99	8.795017296029729E-4
100-101	1.5077172507479535E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1989763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.63817294044047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.40483524094051	49.73773802129215
2	11.640058733005308	14.582240239097418
3	3.6653620215256657	6.887747405809415
4	1.695781375697056	4.248825883203609
5	0.945850310086885	2.962316764949578
6	0.5944305115057849	2.2340424708464304
7	0.4089745228817088	1.7932191824749026
8	0.2921872700239477	1.4641661400604171
9	0.2230229061372135	1.2572772627872164
>10	1.080750657593594	11.258494553321016
>50	0.0364255746982061	1.5083410068194494
>100	0.010711238638801614	1.2491123459129023
>500	0.0012072279489734072	0.4887479145227702
>1k	4.0240931632444385E-4	0.3277308089027717
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.025724169159845E-5
2	0.0	0.0	0.0	0.0	5.025724169159845E-5
3	0.0	0.0	0.0	0.0	5.025724169159845E-5
4	0.0	0.0	0.0	0.0	5.025724169159845E-5
5	0.0	5.025724169159845E-5	0.0	0.0	5.025724169159845E-5
6	0.0	5.025724169159845E-5	0.0	0.0	1.005144833831969E-4
7	0.0	1.005144833831969E-4	0.0	0.0	1.005144833831969E-4
8	0.0	1.005144833831969E-4	0.0	0.0	1.005144833831969E-4
9	0.0	1.005144833831969E-4	0.0	0.0	1.005144833831969E-4
10-11	0.0	1.005144833831969E-4	0.0	0.0	1.5077172507479533E-4
12-13	0.0	1.256431042289961E-4	0.0	0.0	2.7641482930379144E-4
14-15	0.0	1.5077172507479533E-4	0.0	0.0	6.030869002991814E-4
16-17	1.005144833831969E-4	1.5077172507479533E-4	0.0	2.5128620845799223E-5	7.538586253739767E-4
18-19	1.005144833831969E-4	1.5077172507479533E-4	0.0	5.025724169159845E-5	8.041158670655751E-4
20-21	1.005144833831969E-4	1.5077172507479533E-4	0.0	5.025724169159845E-5	8.543731087571736E-4
22-23	1.005144833831969E-4	1.5077172507479533E-4	0.0	1.5077172507479533E-4	9.800162129861697E-4
24-25	1.005144833831969E-4	1.5077172507479533E-4	0.0	4.020579335327876E-4	0.0011056593172151657
26-27	1.005144833831969E-4	1.5077172507479533E-4	0.0	0.0012564310422899612	0.001155916558906764
28-29	1.005144833831969E-4	1.5077172507479533E-4	0.0	0.006558570040753598	0.0012061738005983626
30-31	1.005144833831969E-4	1.5077172507479533E-4	0.0	0.02857124190167372	0.0012313024214441618
32-33	1.256431042289961E-4	1.5077172507479533E-4	0.0	0.0803613294648659	0.0012564310422899612
34-35	1.5077172507479533E-4	1.5077172507479533E-4	0.0	0.16758277242063502	0.0014574600090563549
36-37	1.5077172507479533E-4	1.7590034592059457E-4	0.0	0.2975479994351086	0.0014574600090563549
38-39	1.5077172507479533E-4	2.010289667663938E-4	0.0	0.4887516754507949	0.0015077172507479534
40-41	1.5077172507479533E-4	2.010289667663938E-4	0.0	0.6994049040011299	0.0015077172507479534
42-43	1.5077172507479533E-4	2.512862084579922E-4	0.0	0.924783504367103	0.0015077172507479534
44-45	1.5077172507479533E-4	2.512862084579922E-4	0.0	1.2094907785500082	0.001557974492439552
46-47	1.5077172507479533E-4	2.512862084579922E-4	0.0	1.5435255354532174	0.0016584889758227486
48-49	1.5077172507479533E-4	2.512862084579922E-4	0.0	1.871956609907813	0.0017590034592059457
50-51	1.5077172507479533E-4	2.512862084579922E-4	0.0	2.198201494348824	0.0018595179425891426
52-53	1.5077172507479533E-4	2.7641482930379144E-4	0.0	2.527788485362327	0.00193490380512654
54-55	1.5077172507479533E-4	3.0154345014959065E-4	0.0	2.9171815939888317	0.0020102896676639378
56-57	1.5077172507479533E-4	3.5180069184118914E-4	0.0	3.375678409941284	0.0020102896676639378
58-59	1.5077172507479533E-4	4.52315175224386E-4	0.0	3.8416132976640935	0.0020102896676639378
60-61	1.5077172507479533E-4	4.52315175224386E-4	0.0	4.312498523693526	0.0020102896676639378
62-63	1.5077172507479533E-4	4.52315175224386E-4	0.0	4.783936579381564	0.0020102896676639378
64-65	1.5077172507479533E-4	4.774437960701852E-4	0.0	5.313497135085937	0.002110804151047135
66-67	2.010289667663938E-4	5.025724169159844E-4	0.0	5.938646964487731	0.002110804151047135
68-69	2.010289667663938E-4	5.528296586075829E-4	0.0	6.59761991754797	0.002161061392738733
70-71	2.010289667663938E-4	5.528296586075829E-4	0.0	7.254557452319698	0.002161061392738733
72-73	2.010289667663938E-4	5.528296586075829E-4	0.0	7.9374277238042925	0.0025379907054257217
74-75	2.512862084579922E-4	5.528296586075829E-4	0.0	8.66472539694426	0.0025631193262715206
76-77	2.512862084579922E-4	5.528296586075829E-4	0.0	9.478415268552084	0.0025631193262715206
78-79	2.512862084579922E-4	5.528296586075829E-4	0.0	10.355102592620327	0.0026133765679631194
80-81	2.512862084579922E-4	5.77958279453382E-4	0.0	11.243625497106942	0.0026636338096547177
82-83	2.512862084579922E-4	6.030869002991813E-4	0.0	12.144838355120685	0.0029651772598043085
84-85	2.512862084579922E-4	6.282155211449806E-4	0.0	13.10125879313265	0.0029651772598043085
86-87	2.512862084579922E-4	7.036013836823783E-4	0.0	14.121631571197172	0.003065691743187505
88-89	2.512862084579922E-4	7.036013836823783E-4	0.0	15.222516450451636	0.0031662062265707023
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1595	0.0	52.744926	1
GTATCAA	2915	0.0	41.089428	1
TCAACGC	3690	0.0	32.02152	4
TATCAAC	3740	0.0	31.466543	2
CAACGCA	3755	0.0	31.366863	5
ATCAACG	3800	0.0	31.219456	3
AACGCAG	4105	0.0	28.574614	6
GTACATG	4715	0.0	28.326487	1
TACATGG	5035	0.0	26.389278	2
ACATGGG	5190	0.0	24.778265	3
TAAGGCG	615	0.0	23.939545	5
ACGCAGA	4840	0.0	23.744692	7
CGCAGAG	5055	0.0	21.983217	8
CATGGGG	4575	0.0	21.885693	4
TAAGGTA	495	0.0	21.090477	4
TCTATAC	440	0.0	20.491318	3
TAAGGTG	1050	0.0	19.901815	5
GCAGAGT	5465	0.0	19.292175	9
GTATTAA	1140	0.0	19.178785	1
GGTAAGG	855	0.0	18.870428	3
>>END_MODULE