FastQCFastQC Report
Mon 15 Aug 2016
SRR2921706_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921706_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5943281
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77100.042.415331
GGTATCA41550.042.1515271
TCAACGC84000.037.2957344
CAACGCA87350.035.861465
ATCAACG89500.034.8432463
TATCAAC93700.033.686972
AACGCAG97550.032.3386576
ACGCAGA115900.026.362847
GTACATG156950.025.8258421
CGCAGAG122450.024.6437268
TACATGG163900.023.8921322
ACATGGG163300.022.8751953
CATGGGG140550.019.6893884
GCAGAGT144550.019.5308699
AGAGTAC116850.018.29339210-11
TAAGGCG13350.016.3539835
GAGTACA118300.016.289181
CAGAGTA133850.015.93453810-11
AGTACAT117100.015.6057172
GTATTAA33250.015.5593991
Produced by FastQC (version 0.11.5)