##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921706_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5943281
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.359119819507104	31.0	31.0	34.0	26.0	34.0
2	30.41895781135033	31.0	31.0	34.0	26.0	34.0
3	30.480311632581397	33.0	31.0	34.0	26.0	34.0
4	33.926538388476	37.0	35.0	37.0	30.0	37.0
5	33.91998140421091	37.0	35.0	37.0	30.0	37.0
6	33.9596312205329	37.0	35.0	37.0	30.0	37.0
7	33.87688164163868	37.0	35.0	37.0	29.0	37.0
8	33.84614659815008	37.0	35.0	37.0	29.0	37.0
9	35.40435964579161	39.0	37.0	39.0	30.0	39.0
10-11	35.29919567996196	39.0	35.5	39.0	29.0	39.0
12-13	35.20057456479006	39.0	35.0	39.0	29.0	39.0
14-15	36.34335344399835	40.0	37.0	41.0	28.0	41.0
16-17	36.285456551692576	40.0	37.0	41.0	28.0	41.0
18-19	36.24806979511822	40.0	37.0	41.0	27.0	41.0
20-21	36.174117629639255	40.0	37.0	41.0	27.0	41.0
22-23	36.07723461165642	40.0	36.5	41.0	27.0	41.0
24-25	35.96970149989543	40.0	36.0	41.0	27.0	41.0
26-27	35.828211975842976	40.0	36.0	41.0	27.0	41.0
28-29	35.691964505800755	40.0	36.0	41.0	26.5	41.0
30-31	35.552364846959115	40.0	36.0	41.0	25.5	41.0
32-33	35.38216029159651	39.5	35.5	41.0	25.0	41.0
34-35	35.21573462536939	39.0	35.0	41.0	25.0	41.0
36-37	35.19015372148818	39.0	35.0	41.0	24.5	41.0
38-39	35.37106843846017	40.0	35.0	41.0	25.0	41.0
40-41	35.43929472626316	40.0	35.0	41.0	25.0	41.0
42-43	35.28822244817299	40.0	35.0	41.0	24.0	41.0
44-45	35.17541371508432	40.0	35.0	41.0	24.0	41.0
46-47	35.031272709468055	40.0	35.0	41.0	23.0	41.0
48-49	34.87356890915977	39.5	35.0	41.0	22.5	41.0
50-51	33.19404618425412	37.0	32.5	39.5	18.5	40.0
52-53	33.31153852560564	37.5	33.5	39.5	19.0	40.0
54-55	34.22803607973441	39.0	34.0	40.5	20.0	41.0
56-57	34.25693737179851	39.0	34.0	41.0	20.0	41.0
58-59	34.07597032682789	38.5	34.0	41.0	19.0	41.0
60-61	33.8314903838469	38.0	34.0	40.5	18.0	41.0
62-63	33.54709099233235	37.5	34.0	40.0	15.5	41.0
64-65	33.263793433290466	37.0	33.5	40.0	13.0	41.0
66-67	32.92814886255589	36.5	33.0	39.5	12.0	41.0
68-69	32.57191128940395	36.0	33.0	39.0	8.0	41.0
70-71	32.16278171939035	35.5	32.5	39.0	7.0	40.5
72-73	31.803458813406266	35.0	32.0	37.5	2.0	40.0
74-75	31.403141884087255	35.0	32.0	37.0	2.0	39.0
76-77	31.01961273579358	35.0	32.0	37.0	2.0	39.0
78-79	30.676186268157267	35.0	31.5	36.0	2.0	38.5
80-81	30.36020920094473	35.0	31.0	36.0	2.0	37.0
82-83	30.050722067491	35.0	31.0	35.0	2.0	37.0
84-85	29.790324906394297	35.0	31.0	35.0	2.0	36.5
86-87	29.560442371814492	35.0	31.0	35.0	2.0	36.0
88-89	29.34803309821629	35.0	31.0	35.0	2.0	36.0
90-91	29.134410353473108	34.5	30.5	35.0	2.0	35.5
92-93	28.953663388959733	34.0	30.0	35.0	2.0	35.0
94-95	28.73653382702248	34.0	30.0	35.0	2.0	35.0
96-97	28.51404165476948	34.0	30.0	35.0	2.0	35.0
98-99	28.25777874207866	34.0	30.0	35.0	2.0	35.0
100-101	27.19903273966013	33.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	210324.0
3	35333.0
4	23758.0
5	18040.0
6	16688.0
7	16517.0
8	17935.0
9	18764.0
10	20831.0
11	22080.0
12	22037.0
13	22228.0
14	23489.0
15	24531.0
16	24594.0
17	25391.0
18	24744.0
19	23479.0
20	24145.0
21	25268.0
22	27391.0
23	31403.0
24	37147.0
25	43837.0
26	52441.0
27	61657.0
28	72957.0
29	86626.0
30	103946.0
31	125907.0
32	157845.0
33	204348.0
34	282234.0
35	414067.0
36	691589.0
37	1261972.0
38	1404840.0
39	242876.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.560058617999026	17.997981534151887	13.33626816135269	25.105691686496396
2	15.742728082631066	19.71243532345722	39.29550493292027	25.249331660991448
3	19.044107259906713	23.894978112302017	28.998072293132672	28.0628423346586
4	13.3799883418603	16.121014992259898	36.76750631987686	33.73149034600294
5	14.683161367151767	36.72541347732439	34.235533534036776	14.355891621487066
6	33.307699737594994	35.13305855464886	17.55626462082527	14.002977086930871
7	29.080317932406132	31.227352835606304	21.264459982556225	18.427869249431346
8	26.500670442505513	34.27502682443157	20.26202118221362	18.962281550849294
9	26.690000910328287	15.92071629543165	19.3612524232653	38.02803037097476
10-11	25.48581686850875	25.14170342297717	28.031522669602797	21.34095703891129
12-13	26.282814916202618	22.54568614863577	27.548655276021982	23.622843659139633
14-15	23.49438413880899	24.02735526240708	24.882817385326668	27.59544321345726
16-17	23.806604577384046	26.972173426432196	25.63746562964802	23.58375636653574
18-19	23.77081490213005	26.503298370313534	26.672741338859435	23.05314538869699
20-21	24.38265234298316	25.77083312472279	26.476123272042084	23.370391260251964
22-23	24.177229443612394	25.58176486489307	26.216381485996138	24.0246242054984
24-25	24.00595458605629	25.78957848135333	26.21353430383874	23.990932628751636
26-27	23.921776550518008	25.91752566389285	26.16346753467172	23.997230250917422
28-29	23.989666736866532	26.056167913590723	26.041283747154946	23.912881602387795
30-31	24.227030690924853	25.82873641728471	25.948777052895117	23.995455838895317
32-33	24.009294689149304	25.849958146470442	25.89184112368208	24.248906040698174
34-35	24.30040156858258	25.94170062149321	25.86052033124991	23.897377478674294
36-37	24.3671697283662	25.7420594034517	25.858746587825276	24.03202428035682
38-39	24.146246763544738	25.887687903702446	25.933800686938284	24.032264645814536
40-41	24.37243057494043	25.703193578820322	25.996116238253187	23.928259607986057
42-43	24.215084666966703	25.660664719283993	25.88389763644761	24.240352977301686
44-45	24.154680336912445	25.75068209345586	25.965470941596617	24.129166628035073
46-47	24.3349913558612	25.74512813639785	25.70629483804567	24.213585669695277
48-49	24.110032703471525	25.77113639400286	25.802883081401255	24.31594782112436
50-51	24.17961226057089	25.825896144108295	25.865226237808493	24.129265357512324
52-53	24.3381269414138	25.69882467148806	25.905369309179754	24.05767907791838
54-55	24.04987906773968	25.56483453568764	26.061418398478057	24.32386799809462
56-57	24.024851518641633	25.71299164785247	26.02183590792061	24.24032092558529
58-59	24.120051485658674	25.56032130989659	26.035614398449358	24.284012805995374
60-61	24.0696320411795	25.5753519219764	26.15220450542413	24.202811531419975
62-63	24.04705517405028	25.632965090475967	26.155105391974015	24.164874343499736
64-65	23.954504770830372	25.500630777668025	26.084135945863885	24.460728505637718
66-67	23.95102857046041	25.641183852159838	25.967607787543663	24.44017978983609
68-69	24.088773559436152	25.54395096486568	26.03457034623195	24.332705129466216
70-71	23.965714345434233	25.37358920055316	26.067408552146738	24.593287901865875
72-73	23.976874765511276	25.44563920308352	26.01073858246969	24.566747448935512
74-75	24.028841221222965	25.555041195362598	25.97560089530142	24.440516688113014
76-77	24.024461701638444	25.325915526956894	26.11884909035618	24.530773681048483
78-79	24.001062190852934	25.371490713206363	26.0988301147967	24.528616981144005
80-81	24.09673618894529	25.451642444230448	25.967280109116892	24.484341257707378
82-83	24.070105932517762	25.49401676196951	26.050363135537776	24.38551416997495
84-85	24.037825853910956	25.485569938004527	26.065927073334528	24.41067713474999
86-87	24.041180123621317	25.50102638325644	26.019332009792905	24.438461483329334
88-89	24.066239326868573	25.310739245801212	26.021897714827873	24.60112371250234
90-91	23.960689869981994	25.405746600558427	26.09026402092052	24.543299508539057
92-93	24.036597925757793	25.293655648475767	26.225289802436464	24.444456623329977
94-95	23.970274681952606	25.18416642994239	26.231748305134268	24.613810582970743
96-97	24.03278790542918	25.12795548564901	26.25466027636209	24.584596332559716
98-99	23.954245985250303	25.01752985387235	26.21009955675356	24.81812460412379
100-101	24.159353957497718	24.869479251008134	26.300936484371324	24.67023030712282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	35.5
2	54.5
3	94.0
4	85.0
5	99.0
6	183.0
7	371.5
8	602.5
9	719.5
10	697.0
11	684.5
12	767.0
13	891.0
14	1073.5
15	1256.0
16	1435.0
17	1628.5
18	1757.5
19	1905.5
20	2103.5
21	2305.5
22	2517.5
23	2739.0
24	3105.0
25	3650.5
26	4453.0
27	5525.0
28	7050.5
29	9747.0
30	14252.5
31	21562.5
32	32781.5
33	45791.0
34	60107.5
35	77136.5
36	98364.5
37	119681.0
38	138998.0
39	156177.0
40	170219.0
41	184844.5
42	200013.5
43	214455.0
44	228140.5
45	243892.5
46	254652.0
47	260744.0
48	267152.5
49	269411.0
50	265435.5
51	260198.0
52	257743.0
53	252702.5
54	245511.0
55	233444.0
56	214626.5
57	192063.0
58	172874.0
59	152491.0
60	125551.5
61	102576.0
62	83940.5
63	64672.0
64	47757.0
65	35698.0
66	27171.5
67	21618.5
68	17019.0
69	13153.5
70	10205.0
71	7598.0
72	5481.0
73	4285.0
74	3441.5
75	2656.5
76	2029.0
77	1430.0
78	952.0
79	671.0
80	468.0
81	334.5
82	263.5
83	210.5
84	167.0
85	144.5
86	128.0
87	106.0
88	90.5
89	74.5
90	67.5
91	61.0
92	44.0
93	37.0
94	39.5
95	32.0
96	20.5
97	19.0
98	17.0
99	13.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05259721019416715
2	0.07241791192440673
3	0.06898546442613095
4	0.06869942713460797
5	0.06457712499207087
6	0.009405579174196878
7	0.012787549503380373
8	0.015765702479825536
9	0.00622551752138255
10-11	0.012854852395503425
12-13	0.02186502707847736
14-15	0.03937219189198693
16-17	0.02639114657375278
18-19	0.00964955215814295
20-21	0.019980546099031833
22-23	0.035922918670680384
24-25	0.0332812801548505
26-27	0.020636749297231614
28-29	0.012190236335788262
30-31	0.01194626335184219
32-33	0.008959697513881641
34-35	0.005653442938336585
36-37	6.814417827459277E-4
38-39	0.0040970635579909475
40-41	0.0012451035042765099
42-43	0.21752294734171243
44-45	0.18804428059181452
46-47	0.19376502642227414
48-49	0.20112628024823326
50-51	0.19242738144132845
52-53	0.1998138738518337
54-55	0.1960953890620349
56-57	0.19875385330089557
58-59	0.20710782478566975
60-61	0.21138155843548367
62-63	0.19742462118146525
64-65	0.19709651958236535
66-67	0.19479980838866615
68-69	0.18995400015580619
70-71	0.18220575470013953
72-73	0.19336962193105123
74-75	0.1922170599034439
76-77	0.1862439282275228
78-79	0.20432316762407834
80-81	0.21469622587254414
82-83	0.21390541689009826
84-85	0.20803323955236172
86-87	0.2267855078701478
88-89	0.21919710678327342
90-91	0.22563294584254048
92-93	0.22148540511545728
94-95	0.22523754135131754
96-97	0.23181639905634613
98-99	0.2254142114431406
100-101	0.23158925179543083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5943281.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.55794382676271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84556870587933	40.83420333137473
2	13.37060342471924	14.58945261151507
3	4.8135207263735955	7.878473801953459
4	2.2224764541477664	4.850149821667864
5	1.2575840384906878	3.4305599664704167
6	0.8010513766021836	2.6222229604207725
7	0.5192588675184484	1.983078728793395
8	0.36447517546707014	1.5908012919505516
9	0.27070144340951163	1.3292022729023747
>10	1.417755382618532	13.724521816557184
>50	0.08168476704224766	3.042710249434237
>100	0.03349341169711039	3.0696277578973823
>500	0.001145267907092728	0.43788917629364305
>1k	6.809581270207717E-4	0.617106212768964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6825723030763647E-5	0.0
5	0.0	0.0	0.0	1.6825723030763647E-5	0.0
6	0.0	0.0	0.0	1.6825723030763647E-5	0.0
7	0.0	0.0	0.0	1.6825723030763647E-5	0.0
8	0.0	0.0	0.0	1.6825723030763647E-5	0.0
9	0.0	0.0	0.0	3.3651446061527294E-5	0.0
10-11	0.0	8.412861515381824E-6	0.0	3.3651446061527294E-5	0.0
12-13	0.0	1.6825723030763647E-5	0.0	1.0095433818458188E-4	3.3651446061527294E-5
14-15	0.0	1.6825723030763647E-5	0.0	1.0095433818458188E-4	7.571575363843641E-5
16-17	8.412861515381824E-6	1.6825723030763647E-5	0.0	1.2619292273072734E-4	1.1778006121534552E-4
18-19	1.6825723030763647E-5	1.6825723030763647E-5	0.0	1.8508295333840013E-4	1.1778006121534552E-4
20-21	1.6825723030763647E-5	2.523858454614547E-5	0.0	1.9349581485378196E-4	1.5984436879225462E-4
22-23	1.6825723030763647E-5	3.3651446061527294E-5	0.0	2.1032153788454557E-4	1.9349581485378196E-4
24-25	1.6825723030763647E-5	3.3651446061527294E-5	0.0	3.7016590667680025E-4	2.0190867636916377E-4
26-27	1.6825723030763647E-5	3.3651446061527294E-5	0.0	5.804874445613458E-4	2.1032153788454557E-4
28-29	1.6825723030763647E-5	3.3651446061527294E-5	0.0	0.0018340038103532375	2.2714726091530924E-4
30-31	1.6825723030763647E-5	3.3651446061527294E-5	0.0	0.007487446748689823	2.3556012243069105E-4
32-33	1.6825723030763647E-5	3.3651446061527294E-5	0.0	0.02311854344426925	2.523858454614547E-4
34-35	1.6825723030763647E-5	3.3651446061527294E-5	0.0	0.04418434867878534	2.6921156849221836E-4
36-37	1.6825723030763647E-5	3.3651446061527294E-5	0.0	0.08123459079252689	2.6921156849221836E-4
38-39	3.3651446061527294E-5	3.3651446061527294E-5	0.0	0.1426064155472373	3.1968873758450925E-4
40-41	6.730289212305459E-5	3.3651446061527294E-5	0.0	0.21238268895581414	3.365144606152729E-4
42-43	6.730289212305459E-5	3.3651446061527294E-5	0.0	0.287711450964543	3.365144606152729E-4
44-45	6.730289212305459E-5	3.3651446061527294E-5	0.0	0.3818934356292425	3.365144606152729E-4
46-47	6.730289212305459E-5	3.3651446061527294E-5	0.0	0.4917654070201291	3.4492732213065475E-4
48-49	6.730289212305459E-5	3.3651446061527294E-5	0.0	0.6091921280518287	3.533401836460366E-4
50-51	6.730289212305459E-5	3.3651446061527294E-5	0.0	0.7325246778673262	3.533401836460366E-4
52-53	6.730289212305459E-5	3.3651446061527294E-5	0.0	0.8600047684099069	3.869916297075639E-4
54-55	6.730289212305459E-5	3.3651446061527294E-5	0.0	1.0083487555106347	4.0381735273832753E-4
56-57	6.730289212305459E-5	3.3651446061527294E-5	0.0	1.1792307986110702	4.0381735273832753E-4
58-59	6.730289212305459E-5	3.3651446061527294E-5	0.0	1.363270557121563	4.0381735273832753E-4
60-61	6.730289212305459E-5	3.3651446061527294E-5	0.0	1.5584994214475136	4.0381735273832753E-4
62-63	6.730289212305459E-5	3.3651446061527294E-5	0.0	1.761964477197023	4.374687987998548E-4
64-65	6.730289212305459E-5	3.3651446061527294E-5	0.0	1.9858980250134564	4.374687987998548E-4
66-67	6.730289212305459E-5	3.3651446061527294E-5	0.0	2.24431589218144	4.374687987998548E-4
68-69	6.730289212305459E-5	3.3651446061527294E-5	0.0	2.523328444339078	4.374687987998548E-4
70-71	6.730289212305459E-5	3.3651446061527294E-5	0.0	2.8194191053729414	4.4588166031523665E-4
72-73	6.730289212305459E-5	3.3651446061527294E-5	0.0	3.1285244631711002	5.300102754690549E-4
74-75	6.730289212305459E-5	3.3651446061527294E-5	0.0	3.457635605652837	5.552488600152004E-4
76-77	6.730289212305459E-5	3.3651446061527294E-5	0.0	3.821542343362194	5.552488600152004E-4
78-79	6.730289212305459E-5	3.3651446061527294E-5	0.0	4.229692319780943	5.552488600152004E-4
80-81	6.730289212305459E-5	3.3651446061527294E-5	0.0	4.655913122734733	5.72074583045964E-4
82-83	6.730289212305459E-5	3.3651446061527294E-5	0.0	5.098993300165347	6.309646136536367E-4
84-85	6.730289212305459E-5	3.3651446061527294E-5	0.0	5.5648302679950685	6.477903366844005E-4
86-87	7.571575363843641E-5	3.3651446061527294E-5	0.0	6.067271596278218	6.562031981997823E-4
88-89	8.412861515381823E-5	3.3651446061527294E-5	0.0	6.614452522100167	6.730289212305458E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7710	0.0	42.41533	1
GGTATCA	4155	0.0	42.151527	1
TCAACGC	8400	0.0	37.295734	4
CAACGCA	8735	0.0	35.86146	5
ATCAACG	8950	0.0	34.843246	3
TATCAAC	9370	0.0	33.68697	2
AACGCAG	9755	0.0	32.338657	6
ACGCAGA	11590	0.0	26.36284	7
GTACATG	15695	0.0	25.825842	1
CGCAGAG	12245	0.0	24.643726	8
TACATGG	16390	0.0	23.892132	2
ACATGGG	16330	0.0	22.875195	3
CATGGGG	14055	0.0	19.689388	4
GCAGAGT	14455	0.0	19.530869	9
AGAGTAC	11685	0.0	18.293392	10-11
TAAGGCG	1335	0.0	16.353983	5
GAGTACA	11830	0.0	16.28918	1
CAGAGTA	13385	0.0	15.934538	10-11
AGTACAT	11710	0.0	15.605717	2
GTATTAA	3325	0.0	15.559399	1
>>END_MODULE