FastQCFastQC Report
Mon 15 Aug 2016
SRR2921706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5943281
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74100.041.6315351
GGTATCA38850.040.3755871
TCAACGC86950.034.715344
CAACGCA89150.033.7783285
ATCAACG91350.032.9921073
TATCAAC92850.032.561352
AACGCAG99500.030.4027926
ACGCAGA118150.025.1611467
CGCAGAG124900.023.5352068
GTACATG150100.023.3706911
TACATGG157850.021.738952
ACATGGG162500.020.5619453
GCAGAGT149000.018.6769039
CATGGGG138400.018.0720924
AGAGTAC122400.018.04118310-11
GTATAGC22500.017.1119061
TAAGGCG15550.016.799745
TAATACT37850.016.050114
GAGTACA116700.015.5185111
GTATATA18700.015.5054431
Produced by FastQC (version 0.11.5)