##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921706_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5943281
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300684251678494	33.0	31.0	34.0	28.0	34.0
2	31.447034222342843	34.0	31.0	34.0	28.0	34.0
3	31.518896212378316	34.0	31.0	34.0	28.0	34.0
4	34.99199196538074	37.0	35.0	37.0	32.0	37.0
5	34.72575754032158	37.0	35.0	37.0	32.0	37.0
6	34.69800872615648	37.0	35.0	37.0	32.0	37.0
7	34.58587234896011	37.0	35.0	37.0	32.0	37.0
8	34.5289790269045	37.0	35.0	37.0	32.0	37.0
9	36.1152560345035	39.0	37.0	39.0	32.0	39.0
10-11	36.003651854926595	39.0	37.0	39.0	31.0	39.0
12-13	35.929491807639586	39.0	37.0	39.0	31.0	39.0
14-15	37.16357723957525	40.0	38.0	41.0	31.0	41.0
16-17	37.13129094855182	40.0	38.0	41.0	31.0	41.0
18-19	37.08988250765865	40.0	38.0	41.0	31.0	41.0
20-21	37.031652718422706	40.0	38.0	41.0	31.0	41.0
22-23	36.93939021224135	40.0	37.5	41.0	30.5	41.0
24-25	36.85758287383686	40.0	37.0	41.0	30.0	41.0
26-27	36.73080584613112	40.0	37.0	41.0	30.0	41.0
28-29	36.6103383804333	40.0	37.0	41.0	30.0	41.0
30-31	36.4593830411182	40.0	37.0	41.0	30.0	41.0
32-33	36.41188495041712	40.0	37.0	41.0	29.5	41.0
34-35	36.549440199781905	40.0	37.0	41.0	30.0	41.0
36-37	36.5484292262136	40.0	37.0	41.0	30.0	41.0
38-39	36.44773837885168	40.0	37.0	41.0	30.0	41.0
40-41	36.36784765855762	40.0	37.0	41.0	29.5	41.0
42-43	36.25073431661737	40.0	36.5	41.0	29.0	41.0
44-45	36.12505743881199	40.0	36.0	41.0	28.5	41.0
46-47	35.98025652833847	40.0	36.0	41.0	28.0	41.0
48-49	35.841893728396826	40.0	36.0	41.0	27.5	41.0
50-51	35.691238644109205	40.0	35.0	41.0	27.0	41.0
52-53	35.52241076940498	39.0	35.0	41.0	27.0	41.0
54-55	35.312677458797594	39.0	35.0	41.0	26.0	41.0
56-57	35.09252826847662	39.0	35.0	41.0	26.0	41.0
58-59	34.85777973479632	38.5	35.0	40.5	26.0	41.0
60-61	34.586329335597625	38.0	34.5	40.0	26.0	41.0
62-63	34.28713407627874	37.5	34.0	40.0	25.0	41.0
64-65	33.95609319162261	37.0	34.0	40.0	24.0	41.0
66-67	33.60839273795064	36.5	34.0	39.5	23.0	41.0
68-69	33.2513090833161	36.0	34.0	39.0	22.5	41.0
70-71	32.85478029728024	35.5	33.0	39.0	22.0	40.0
72-73	32.48978005583111	35.0	33.0	37.5	21.5	40.0
74-75	32.081309970031704	35.0	33.0	37.0	20.0	39.0
76-77	29.371437931337926	33.0	29.0	34.5	13.0	36.5
78-79	30.935576746245047	34.5	31.5	36.0	16.5	37.5
80-81	31.109031610654114	35.0	32.0	36.0	18.0	37.0
82-83	30.927814114796185	35.0	32.0	35.0	17.0	37.0
84-85	30.72406949965852	35.0	32.0	35.0	14.0	36.5
86-87	30.51315384549376	35.0	32.0	35.0	10.5	36.0
88-89	30.244141325304994	35.0	32.0	35.0	6.5	36.0
90-91	30.04638128333491	34.0	31.0	35.0	2.0	35.0
92-93	29.889177122872027	34.0	31.0	35.0	2.0	35.0
94-95	29.783239257911582	34.0	31.0	35.0	2.0	35.0
96-97	29.581864125219724	34.0	31.0	35.0	2.0	35.0
98-99	29.379488535036455	34.0	31.0	35.0	2.0	35.0
100-101	28.2581398220949	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121126.0
3	39966.0
4	21608.0
5	15172.0
6	13111.0
7	12868.0
8	13555.0
9	14454.0
10	16086.0
11	17285.0
12	18355.0
13	19521.0
14	21108.0
15	22220.0
16	23423.0
17	25061.0
18	23740.0
19	21941.0
20	21290.0
21	21056.0
22	22374.0
23	25265.0
24	29894.0
25	35950.0
26	42976.0
27	52650.0
28	63210.0
29	76359.0
30	93376.0
31	115968.0
32	146615.0
33	194580.0
34	274955.0
35	413153.0
36	728544.0
37	1416729.0
38	1526049.0
39	181685.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03532145727974	19.887817299088148	14.01978743088639	26.057073812745724
2	14.816706153900771	21.219998213105207	37.67889769791753	26.28439793507649
3	17.464351503539564	25.253602038273325	28.93605020094479	28.345996257242316
4	12.965559575084946	17.521225644795692	35.82517980611917	33.68803497400019
5	14.353862292172922	37.12752438235655	34.35883040085333	14.159782924617199
6	33.07761009997402	35.95714658773201	17.29769630350535	13.667547008788613
7	28.71986029265653	31.529217615657075	21.45520294261705	18.295719149069345
8	26.399458480929976	34.88386297063861	20.020540842675956	18.696137705755458
9	26.41005868643936	15.842124913831265	19.38012017267903	38.367696227050345
10-11	25.30088680744103	25.51674322277685	28.058472292759408	21.12389767702271
12-13	26.07582298211892	22.799239609749794	27.671865156923868	23.45307225120742
14-15	23.277882700398653	24.29639959858684	24.92089858933294	27.504819111681567
16-17	23.611791501343408	27.303708785072487	25.64454933601064	23.439950377573464
18-19	23.616407216510023	26.786495944299155	26.73206378237904	22.865033056811782
20-21	24.245025608400905	25.965443738874484	26.523680714529867	23.26584993819474
22-23	23.972945246909912	25.76883879459847	26.30868370517901	23.949532253312604
24-25	23.86768583984955	25.99136431941519	26.264068693739738	23.876881146995522
26-27	23.7916632137931	26.156331252074356	26.195258633200503	23.856746900932045
28-29	23.82001120990402	26.274419863909543	26.08282338814722	23.822745538039218
30-31	24.09013333337821	26.05553031741743	25.979961750407316	23.874374598797047
32-33	23.891399768181653	25.992691058300565	25.924808130604465	24.191101042913317
34-35	24.12775479619384	26.095814330266087	25.95466591593929	23.821764957600784
36-37	24.21818993155284	25.92843928746032	25.93506887833632	23.918301902650516
38-39	24.056983344467756	26.05346491722879	25.967233079441566	23.922318658861887
40-41	24.266962899870446	25.873541188465783	26.011256312083386	23.848239599580385
42-43	24.15262882679515	25.86798884517058	25.833080572987022	24.14630175504725
44-45	24.065014315534057	25.91009020090546	25.92716956272627	24.09772592083421
46-47	24.185485293068474	25.94329542685841	25.69587188257608	24.175347397497042
48-49	23.971916911819456	25.935621257765273	25.85554011650384	24.23692171391143
50-51	24.070168991501838	25.951297044081052	25.844433793454417	24.13410017096269
52-53	24.255993732611795	25.84836598733785	25.811886988127753	24.0837532919226
54-55	23.956614928591765	25.72244511705512	25.960380022789792	24.360559931563323
56-57	23.94321332879451	25.860692336596045	25.92888406363125	24.26721027097819
58-59	23.994230544474274	25.727818648868034	25.948986159213515	24.328964647444177
60-61	23.980818838226362	25.772631129045013	25.994049952800108	24.252500079928517
62-63	23.938738178060934	25.75676271755932	26.0280615617224	24.276437542657348
64-65	23.822940220079953	25.637068893107894	25.94407929786372	24.595911588948432
66-67	23.787480362989434	25.84595930048048	25.811647670403627	24.55491266612646
68-69	23.943879513589906	25.69147284081239	25.848991331180226	24.515656314417477
70-71	23.79852983121245	25.524912353249995	25.90241383409365	24.774143981443906
72-73	23.77735772108331	25.62578671213176	25.819238893962165	24.777616672822766
74-75	23.774924128589586	25.758638367371116	25.726753188349598	24.739684315689697
76-77	23.737673011219393	25.48704684968194	25.96708294977268	24.80819718932599
78-79	23.694689846359807	25.547524475288956	25.921397012236064	24.83638866611517
80-81	23.76586266345675	25.665716021608944	25.784147476626245	24.784273838308064
82-83	23.726341485088927	25.66841153193134	25.82245190877107	24.782795074208668
84-85	23.692764162973624	25.613760421532856	25.84247229692478	24.85100311856874
86-87	23.64178562174214	25.640998658593194	25.846402101801107	24.870813617863558
88-89	23.57759165118479	25.468559012132015	25.83337418428524	25.120475152397958
90-91	23.412808105231928	25.59611141656263	25.85189752894786	25.13918294925758
92-93	23.439010744220354	25.483844277278273	25.99932646085481	25.077818517646566
94-95	23.300404139429702	25.36740980734343	26.02122316751955	25.310962885707312
96-97	23.289642418502023	25.34352125175022	26.03758525399441	25.329251075753344
98-99	23.188079156190472	25.26669389088808	25.976904505070213	25.568322447851227
100-101	23.091116246517807	25.252737528941342	26.04057816315108	25.61556806138977
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	37.0
2	52.5
3	69.0
4	59.5
5	78.5
6	128.0
7	310.0
8	474.0
9	492.0
10	452.0
11	398.0
12	398.5
13	428.5
14	509.0
15	589.0
16	650.0
17	765.0
18	881.0
19	988.5
20	1179.0
21	1442.0
22	1709.5
23	2091.5
24	2611.5
25	3348.5
26	4410.0
27	5902.5
28	7947.5
29	11347.5
30	16891.5
31	24997.5
32	37280.5
33	51045.0
34	64806.0
35	81115.5
36	101813.0
37	122108.5
38	140403.5
39	156932.0
40	170898.5
41	185504.0
42	200299.0
43	214897.0
44	228311.0
45	243583.0
46	254223.5
47	260494.5
48	267635.0
49	270041.0
50	266644.0
51	260889.5
52	257115.0
53	253167.5
54	245651.0
55	232658.5
56	214187.5
57	190784.0
58	170651.5
59	150796.5
60	124183.5
61	100762.0
62	81966.0
63	62407.5
64	45571.5
65	33921.0
66	25726.5
67	20413.0
68	15933.0
69	12293.0
70	9439.0
71	6870.0
72	4975.5
73	3779.0
74	2869.5
75	2191.0
76	1663.5
77	1096.5
78	634.5
79	357.0
80	221.0
81	139.0
82	79.0
83	45.0
84	30.5
85	23.5
86	25.0
87	21.0
88	11.5
89	6.5
90	4.5
91	6.0
92	5.0
93	3.5
94	3.0
95	3.0
96	2.0
97	1.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15498173483636396
2	1.6825723030763646E-4
3	0.010162736710581243
4	0.005922654506828804
5	0.02325314922851536
6	0.0023051240552146197
7	0.0
8	0.0
9	0.0
10-11	0.046405344118846135
12-13	2.86037291522982E-4
14-15	0.00466072527952153
16-17	8.412861515381824E-6
18-19	0.0017414623336840375
20-21	2.776244300076002E-4
22-23	0.0
24-25	5.384231369844367E-4
26-27	0.0027510057155298562
28-29	0.005417882815905895
30-31	0.016750007277125212
32-33	0.011727528952442261
34-35	0.010095433818458188
36-37	0.003861503435560257
38-39	8.412861515381824E-6
40-41	0.003625943313129566
42-43	0.009481294927835315
44-45	0.00733601524141295
46-47	0.00392039346616793
48-49	0.009397166312681497
50-51	0.0028856114997759656
52-53	0.002279885470668474
54-55	0.004526119495275421
56-57	0.008042695608705023
58-59	0.016026501186802374
60-61	0.007924915547489678
62-63	0.022033284308784998
64-65	0.0410547641950633
66-67	0.060639905802872186
68-69	0.0509146378910908
70-71	0.06896863870310019
72-73	0.033979547660627184
74-75	0.027745617277729256
76-77	0.22045062314906533
78-79	0.0823366756510419
80-81	0.0669747905239547
82-83	0.027880223061975365
84-85	0.0514194095820137
86-87	0.06720193778487
88-89	0.29052134671068053
90-91	0.2965870198632708
92-93	0.05077161924532931
94-95	0.008917633206304733
96-97	0.013755028577649282
98-99	7.403318133536005E-4
100-101	1.766700918230183E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5943281.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.600894598624635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94269535364519	39.63394619988948
2	13.783442321902825	14.776096782050708
3	5.012769908608097	8.060668545553797
4	2.304220004802468	4.940330144378378
5	1.348376282153585	3.6137087489499833
6	0.8183323943819485	2.6318009050744173
7	0.5261886671456356	1.9742928300664802
8	0.3896803383648684	1.6709771795081356
9	0.28725596482508914	1.3857459024074248
>10	1.4643338167206372	14.013036738039816
>50	0.08657632196324308	3.176824731461668
>100	0.03423846577998679	3.0733500239172686
>500	0.0012286117345919477	0.469187243916296
>1k	6.615479718266417E-4	0.5800340247861714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6825723030763647E-5
2	0.0	0.0	0.0	3.3651446061527294E-5	1.6825723030763647E-5
3	0.0	0.0	0.0	3.3651446061527294E-5	1.6825723030763647E-5
4	0.0	0.0	0.0	3.3651446061527294E-5	3.3651446061527294E-5
5	0.0	0.0	0.0	3.3651446061527294E-5	3.3651446061527294E-5
6	0.0	0.0	0.0	3.3651446061527294E-5	3.3651446061527294E-5
7	0.0	0.0	0.0	3.3651446061527294E-5	3.3651446061527294E-5
8	0.0	0.0	0.0	3.3651446061527294E-5	3.3651446061527294E-5
9	0.0	0.0	0.0	3.3651446061527294E-5	5.047716909229094E-5
10-11	1.6825723030763647E-5	0.0	0.0	4.2064307576909115E-5	5.047716909229094E-5
12-13	1.6825723030763647E-5	0.0	0.0	6.730289212305459E-5	9.254147666920006E-5
14-15	1.6825723030763647E-5	0.0	0.0	7.571575363843641E-5	1.1778006121534554E-4
16-17	1.6825723030763647E-5	0.0	0.0	1.093671996999637E-4	1.6825723030763646E-4
18-19	1.6825723030763647E-5	0.0	0.0	1.6825723030763646E-4	1.766700918230183E-4
20-21	1.6825723030763647E-5	0.0	0.0	1.766700918230183E-4	1.9349581485378196E-4
22-23	1.6825723030763647E-5	0.0	0.0	2.1032153788454557E-4	2.1032153788454557E-4
24-25	1.6825723030763647E-5	0.0	0.0	3.617530451614184E-4	2.2714726091530924E-4
26-27	2.523858454614547E-5	0.0	0.0	5.468359984998185E-4	2.3556012243069105E-4
28-29	3.3651446061527294E-5	1.6825723030763647E-5	0.0	0.0018424166718686193	2.3556012243069105E-4
30-31	3.3651446061527294E-5	1.6825723030763647E-5	0.0	0.007579988225359022	2.3556012243069105E-4
32-33	3.3651446061527294E-5	1.6825723030763647E-5	0.0	0.023480296489430667	2.523858454614547E-4
34-35	3.3651446061527294E-5	1.6825723030763647E-5	0.0	0.04459657889303904	2.6921156849221836E-4
36-37	3.3651446061527294E-5	1.6825723030763647E-5	0.0	0.08182349109860362	2.6921156849221836E-4
38-39	4.2064307576909115E-5	1.6825723030763647E-5	0.0	0.1431532515457371	3.1127587606912747E-4
40-41	5.047716909229094E-5	1.6825723030763647E-5	0.0	0.21362779246009064	3.196887375845093E-4
42-43	5.047716909229094E-5	1.6825723030763647E-5	0.0	0.2894108489906501	3.2810159909989114E-4
44-45	5.047716909229094E-5	1.6825723030763647E-5	0.0	0.3838956966699034	3.4492732213065475E-4
46-47	5.047716909229094E-5	1.6825723030763647E-5	0.0	0.4943481555053514	3.954044912229457E-4
48-49	5.047716909229094E-5	1.6825723030763647E-5	0.0	0.6110429575852125	4.542945218306185E-4
50-51	5.047716909229094E-5	1.6825723030763647E-5	0.0	0.7340053414940333	4.542945218306185E-4
52-53	7.571575363843641E-5	1.6825723030763647E-5	0.0	0.8610900275453912	4.7953310637676393E-4
54-55	9.254147666920006E-5	1.6825723030763647E-5	0.0	1.0084328841257886	5.047716909229094E-4
56-57	1.0095433818458188E-4	1.6825723030763647E-5	0.0	1.1789784127656087	5.047716909229094E-4
58-59	1.0095433818458188E-4	1.6825723030763647E-5	0.0	1.3615206819263634	5.131845524382912E-4
60-61	1.0095433818458188E-4	1.6825723030763647E-5	0.0	1.5562700131459373	5.21597413953673E-4
62-63	1.0095433818458188E-4	1.6825723030763647E-5	0.0	1.7598612618181775	5.889003060767276E-4
64-65	1.0095433818458188E-4	1.6825723030763647E-5	0.0	1.9838368739421879	5.889003060767276E-4
66-67	1.0095433818458188E-4	1.6825723030763647E-5	0.0	2.2416490150810637	5.889003060767276E-4
68-69	1.0095433818458188E-4	1.6825723030763647E-5	0.0	2.5203839428086945	5.889003060767276E-4
70-71	1.0095433818458188E-4	1.6825723030763647E-5	0.0	2.8166007967652886	6.309646136536367E-4
72-73	1.0095433818458188E-4	1.6825723030763647E-5	0.0	3.124906932719486	6.982675057766914E-4
74-75	1.0095433818458188E-4	1.6825723030763647E-5	0.0	3.454455544000023	7.066803672920732E-4
76-77	1.0095433818458188E-4	1.6825723030763647E-5	0.0	3.818976420600002	7.066803672920732E-4
78-79	1.0095433818458188E-4	1.6825723030763647E-5	0.0	4.227008616957535	7.066803672920732E-4
80-81	1.0095433818458188E-4	1.6825723030763647E-5	0.0	4.654760560707125	7.235060903228368E-4
82-83	1.0095433818458188E-4	1.6825723030763647E-5	0.0	5.099826173455369	7.908089824458914E-4
84-85	1.0095433818458188E-4	1.6825723030763647E-5	0.0	5.567059676296645	7.908089824458914E-4
86-87	1.0095433818458188E-4	1.6825723030763647E-5	0.0	6.0721174045110775	7.908089824458914E-4
88-89	1.1778006121534552E-4	1.6825723030763647E-5	0.0	6.622385850509172	8.076347054766551E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7410	0.0	41.631535	1
GGTATCA	3885	0.0	40.375587	1
TCAACGC	8695	0.0	34.71534	4
CAACGCA	8915	0.0	33.778328	5
ATCAACG	9135	0.0	32.992107	3
TATCAAC	9285	0.0	32.56135	2
AACGCAG	9950	0.0	30.402792	6
ACGCAGA	11815	0.0	25.161146	7
CGCAGAG	12490	0.0	23.535206	8
GTACATG	15010	0.0	23.370691	1
TACATGG	15785	0.0	21.73895	2
ACATGGG	16250	0.0	20.561945	3
GCAGAGT	14900	0.0	18.676903	9
CATGGGG	13840	0.0	18.072092	4
AGAGTAC	12240	0.0	18.041183	10-11
GTATAGC	2250	0.0	17.111906	1
TAAGGCG	1555	0.0	16.79974	5
TAATACT	3785	0.0	16.05011	4
GAGTACA	11670	0.0	15.518511	1
GTATATA	1870	0.0	15.505443	1
>>END_MODULE