FastQCFastQC Report
Mon 15 Aug 2016
SRR2921705_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921705_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413032
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.048.920421
GTATCAA40400.045.699591
TCAACGC48850.036.1458474
CAACGCA50400.035.1269455
ATCAACG51400.034.3504873
TATCAAC52300.034.3045852
AACGCAG53600.033.453846
GTACATG92300.028.2302471
ACGCAGA64050.027.4789897
TACATGG95500.026.9872422
CGCAGAG66500.026.1828788
ACATGGG99050.025.4923693
GTATAGG6400.024.4724831
CATGGGG81000.023.2640634
GCAGAGT77300.021.4817929
ATGGGGG46550.020.6984775
GTATTAG6350.019.4331721
AGAGTAC65750.019.37510310-11
GTATTAC5450.019.1588841
TACACTG33450.018.5881965
Produced by FastQC (version 0.11.5)