##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921705_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2413032
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.558936640707625	31.0	31.0	34.0	27.0	34.0
2	30.614206110818255	33.0	31.0	34.0	27.0	34.0
3	30.67914018545962	33.0	31.0	34.0	27.0	34.0
4	34.13270565827556	37.0	35.0	37.0	30.0	37.0
5	34.13138325558882	37.0	35.0	37.0	30.0	37.0
6	34.17877176929274	37.0	35.0	37.0	30.0	37.0
7	34.10583199891257	37.0	35.0	37.0	30.0	37.0
8	34.07140394325479	37.0	35.0	37.0	30.0	37.0
9	35.642360731229424	39.0	37.0	39.0	30.0	39.0
10-11	35.53927838503592	39.0	37.0	39.0	30.0	39.0
12-13	35.43550624276843	39.0	37.0	39.0	30.0	39.0
14-15	36.59301907309973	40.0	37.0	41.0	30.0	41.0
16-17	36.53759730496736	40.0	37.0	41.0	29.5	41.0
18-19	36.500335884480606	40.0	37.0	41.0	29.0	41.0
20-21	36.42989877465363	40.0	37.0	41.0	29.0	41.0
22-23	36.33402478707286	40.0	37.0	41.0	28.5	41.0
24-25	36.22673818664651	40.0	36.5	41.0	28.0	41.0
26-27	36.08237022965298	40.0	36.0	41.0	27.0	41.0
28-29	35.949149037393624	40.0	36.0	41.0	27.0	41.0
30-31	35.79871464613814	40.0	36.0	41.0	27.0	41.0
32-33	35.63013586226789	40.0	35.5	41.0	26.5	41.0
34-35	35.46483594084123	39.0	35.0	41.0	25.5	41.0
36-37	35.44135448680333	39.0	35.0	41.0	25.5	41.0
38-39	35.631998042297	40.0	35.5	41.0	26.5	41.0
40-41	35.6925745286428	40.0	36.0	41.0	26.0	41.0
42-43	35.53924170918579	40.0	35.0	41.0	25.0	41.0
44-45	35.41905909246127	40.0	35.0	41.0	25.0	41.0
46-47	35.26305121523461	40.0	35.0	41.0	24.0	41.0
48-49	35.10524767180874	40.0	35.0	41.0	24.0	41.0
50-51	33.415615499504355	37.0	32.5	39.5	20.5	40.0
52-53	33.54092962712471	37.5	33.5	39.5	21.5	40.0
54-55	34.45941330243445	39.0	34.5	40.5	22.0	41.0
56-57	34.48285020671089	39.0	34.5	41.0	22.0	41.0
58-59	34.293838415735884	38.5	34.0	41.0	22.0	41.0
60-61	34.042936231264235	38.0	34.0	40.5	21.0	41.0
62-63	33.75097097759168	37.5	34.0	40.0	20.0	41.0
64-65	33.462568668795114	37.0	34.0	40.0	19.5	41.0
66-67	33.12564959768457	36.5	33.5	39.5	18.0	41.0
68-69	32.76817858196659	36.0	33.0	39.0	17.0	41.0
70-71	32.35654686717789	35.0	33.0	39.0	13.0	40.5
72-73	31.99780110665752	35.0	33.0	37.5	9.5	40.0
74-75	31.60584795394342	35.0	32.0	37.0	7.0	39.0
76-77	31.235248641543087	35.0	32.0	36.5	4.0	39.0
78-79	30.904251373375903	35.0	32.0	36.0	2.0	38.5
80-81	30.603031580186254	35.0	32.0	36.0	2.0	37.0
82-83	30.30195310298413	35.0	31.5	35.0	2.0	37.0
84-85	30.056211024138925	35.0	31.0	35.0	2.0	36.5
86-87	29.841435795298196	35.0	31.0	35.0	2.0	36.0
88-89	29.644237830248418	35.0	31.0	35.0	2.0	36.0
90-91	29.443860255479414	35.0	31.0	35.0	2.0	35.5
92-93	29.275369327882927	35.0	31.0	35.0	2.0	35.0
94-95	29.068694903341523	34.0	31.0	35.0	2.0	35.0
96-97	28.8572128343097	34.0	30.0	35.0	2.0	35.0
98-99	28.623755300385575	34.0	30.0	35.0	2.0	35.0
100-101	27.58773381372481	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75271.0
3	13119.0
4	9160.0
5	6867.0
6	6408.0
7	6460.0
8	6895.0
9	7360.0
10	8025.0
11	8443.0
12	8451.0
13	8740.0
14	9331.0
15	9872.0
16	9893.0
17	10373.0
18	10042.0
19	9431.0
20	9452.0
21	9871.0
22	10753.0
23	12655.0
24	14587.0
25	17108.0
26	20436.0
27	24186.0
28	28772.0
29	34351.0
30	41064.0
31	50138.0
32	63051.0
33	82357.0
34	117849.0
35	170107.0
36	282458.0
37	526823.0
38	575211.0
39	97658.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70753313441351	17.43564269400902	13.129371412269892	24.72745275930757
2	15.786989669489182	19.024704224896492	40.10605476842096	25.082251337193362
3	19.693884491098622	23.803224136901406	28.303678661243342	28.199212710756633
4	13.491761345257114	15.27485406309252	36.2601168822336	34.97326770941677
5	14.695749247749884	37.21187244757472	33.573785203149	14.518593101526395
6	33.96794384536254	34.49016605837449	17.227779237605304	14.314110858657658
7	29.962933658169828	31.289123683373944	20.275699309611923	18.4722433488443
8	27.135486352550682	34.779318017153194	19.326066520435113	18.759129109861014
9	27.36684747471064	16.089871323468465	18.787652225232005	37.755628976588895
10-11	26.183893020236926	25.076946414790292	27.160654498370718	21.578506066602067
12-13	26.806610336313717	22.297522336320352	27.22576155095898	23.670105776406952
14-15	23.872969913457737	23.787399849547597	24.402103016987358	27.937527220007308
16-17	24.105248976630914	27.04090367376548	25.08786983781543	23.765977511788176
18-19	24.158648142395645	26.453410842223253	26.38858984701789	22.999351168363212
20-21	24.789572957402267	25.50713782538654	26.30836989066892	23.394919326542272
22-23	24.53789065504037	25.222247580290936	26.057384825828418	24.182476938840278
24-25	24.283422954261198	25.537217731387614	26.0104792436107	24.168880070740492
26-27	24.060409987088164	25.764606644932776	26.04166191712798	24.133321450851085
28-29	24.18767401064554	26.009373131668355	25.801000726350352	24.00195213133575
30-31	24.633171283857504	25.648427586804303	25.699759257160064	24.018641872178133
32-33	24.257213284441768	25.64024083827136	25.770295991520303	24.332249885766565
34-35	24.54149157519845	25.761307270425633	25.82486135491009	23.87233979946583
36-37	24.48265394398081	25.805564620987255	25.787495920555735	23.9242855144762
38-39	24.47813727883336	25.77841664722291	25.696214125240424	24.047231948703303
40-41	24.561460218946642	25.60598638711517	25.760005768733464	24.072547625204724
42-43	24.327174227294027	25.564783183738033	25.735320995736448	24.372721593231493
44-45	24.24039826910655	25.662170975248124	25.865586875739815	24.23184387990551
46-47	24.39901652435921	25.55354765726858	25.61476052334924	24.432675295022964
48-49	24.236671498166384	25.623346526511643	25.745116931983837	24.394865043338136
50-51	24.335615983255956	25.656452188935308	25.794843896645375	24.21308793116336
52-53	24.47827191139609	25.569021523236913	25.79160695719129	24.16109960817571
54-55	23.94470430374343	25.320638873188432	26.21146737421857	24.52318944884957
56-57	24.120095885336287	25.497118622387532	25.868296374065775	24.514489118210406
58-59	24.293206416662755	25.525830170177045	25.898825793744905	24.28213761941529
60-61	24.428322894269616	25.421264454827085	26.026507932255427	24.123904718647868
62-63	24.318318206075407	25.39921633120908	26.043012494263685	24.23945296845183
64-65	24.043684756101612	25.23925531420066	26.100819473330983	24.616240456366743
66-67	24.124547956856354	25.449883635747494	25.9055398022223	24.52002860517385
68-69	24.180461981076913	25.39982326051135	25.957325054483604	24.46238970392813
70-71	24.121232857799225	25.22415945524482	26.010303227476765	24.644304459479184
72-73	24.0119843038759	25.52949917005228	25.82862918882733	24.62988733724449
74-75	24.023052921596104	25.509001258085206	25.94747271051436	24.52047310980432
76-77	23.947568833968806	25.058488007155592	26.15692890831522	24.83701425056038
78-79	23.91679043356625	25.151344490628603	26.160231618538788	24.77163345726636
80-81	24.06410281971658	25.403950028993023	25.769415769876836	24.762531381413563
82-83	24.093626836375677	25.478075018686447	25.92607207454558	24.502226070392297
84-85	24.0332890921404	25.43624232156404	25.937231777816866	24.593236808478693
86-87	23.78578895909872	25.545609028437994	26.000067714946663	24.66853429751663
88-89	23.81533146999611	25.20201326822938	26.0701536702807	24.91250159149381
90-91	23.91998884983608	25.129806560857816	26.166389608911324	24.78381498039478
92-93	24.01645920953672	25.116910776437102	26.144562472777938	24.72206754124824
94-95	23.865730677463624	25.03456786174434	26.140220166694256	24.95948129409778
96-97	23.689544125769697	25.047106719907834	26.2105092386503	25.05283991567217
98-99	23.571853041799784	24.854982019577506	26.302679688426583	25.270485250196128
100-101	23.782752124654426	24.825215716325268	26.36661191750776	25.025420241512546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	28.0
2	38.0
3	62.5
4	67.0
5	67.5
6	106.0
7	234.5
8	380.5
9	429.0
10	408.0
11	390.0
12	419.5
13	477.5
14	560.5
15	664.5
16	732.0
17	794.5
18	882.0
19	947.0
20	1018.5
21	1119.5
22	1190.0
23	1330.0
24	1531.0
25	1724.5
26	2011.5
27	2496.0
28	3170.0
29	4155.5
30	5768.5
31	8459.5
32	12773.5
33	17746.0
34	22998.5
35	29124.0
36	37049.0
37	44650.0
38	51810.5
39	59319.5
40	65294.0
41	70567.5
42	75753.0
43	82425.0
44	89356.5
45	96166.5
46	100254.5
47	103306.0
48	107666.0
49	109791.0
50	109652.0
51	108306.5
52	107020.0
53	105273.5
54	103088.0
55	98903.0
56	91312.5
57	82211.0
58	74008.0
59	64915.0
60	53913.0
61	44386.0
62	36433.0
63	28267.0
64	20934.0
65	15607.5
66	11873.5
67	9177.0
68	7213.5
69	5609.0
70	4315.5
71	3252.0
72	2333.0
73	1760.0
74	1403.5
75	1101.0
76	822.0
77	563.0
78	381.5
79	261.0
80	194.0
81	143.5
82	106.0
83	96.5
84	86.5
85	65.0
86	53.5
87	45.5
88	39.5
89	31.0
90	24.0
91	21.5
92	17.0
93	13.0
94	14.0
95	13.5
96	11.5
97	10.5
98	9.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05553179568277586
2	0.07563099038885518
3	0.07127961833908543
4	0.07111385178480849
5	0.06630662171077714
6	0.0110234758594167
7	0.013717182366417021
8	0.017405488199079
9	0.006630662171077715
10-11	0.013012674510740015
12-13	0.0222748807309642
14-15	0.04034343514715097
16-17	0.027227156539987866
18-19	0.009676622605916538
20-21	0.020617215188194767
22-23	0.03785693683299683
24-25	0.03460376820531182
26-27	0.021425327140294867
28-29	0.01179014617294756
30-31	0.012080237642932212
32-33	0.008682023280254882
34-35	0.0052423672790083186
36-37	8.081119521000965E-4
38-39	0.0036883058326619787
40-41	9.945993256616572E-4
42-43	0.23406237463904334
44-45	0.2035406078328012
46-47	0.20892802084680187
48-49	0.2164082366085489
50-51	0.2077054925090094
52-53	0.21518570827075645
54-55	0.21155956489594835
56-57	0.21367308846297933
58-59	0.22225150764681115
60-61	0.2274524332872502
62-63	0.2124712809444715
64-65	0.21317578880014854
66-67	0.21035775737744047
68-69	0.20588206041196305
70-71	0.19715859549313894
72-73	0.2092388331360711
74-75	0.20751900513544785
76-77	0.2008261805065163
78-79	0.21881185164556458
80-81	0.23024974389067365
82-83	0.22952451521571202
84-85	0.22363980253888052
86-87	0.24378043888352913
88-89	0.2363209439410667
90-91	0.24419485526922147
92-93	0.23818581767668226
94-95	0.24079664090654412
96-97	0.2485462273189912
98-99	0.24162547367792886
100-101	0.24838046076471426
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2413032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.740158319889716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3836798786624	49.0116096372406
2	11.563658989803956	14.278842735754244
3	3.7666049267996633	6.9765235352726345
4	1.7319807587292197	4.277310650037789
5	0.9305817139834366	2.8727131175465854
6	0.585548681085719	2.1691120964540938
7	0.3924095780948111	1.6959200634470335
8	0.29164172968627045	1.440480525081345
9	0.22290334647477222	1.23858791112471
>10	1.0602672123810795	11.369590667279715
>50	0.05179280616543676	2.1493396305056796
>100	0.017652967250527746	1.935145791343659
>500	0.0011429465793091204	0.48171469441271736
>1k	1.3446430344698693E-4	0.10310894449922807
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.1441638569235716E-5	0.0
5	0.0	0.0	0.0	4.1441638569235716E-5	0.0
6	0.0	0.0	0.0	4.1441638569235716E-5	0.0
7	0.0	0.0	0.0	4.1441638569235716E-5	0.0
8	0.0	0.0	0.0	4.1441638569235716E-5	0.0
9	0.0	0.0	0.0	4.1441638569235716E-5	0.0
10-11	0.0	0.0	0.0	4.1441638569235716E-5	0.0
12-13	0.0	0.0	0.0	4.1441638569235716E-5	0.0
14-15	0.0	0.0	0.0	4.1441638569235716E-5	0.0
16-17	0.0	0.0	0.0	8.288327713847143E-5	0.0
18-19	0.0	0.0	0.0	1.45045734992325E-4	0.0
20-21	0.0	2.0720819284617858E-5	0.0	1.8648737356156072E-4	6.216245785385358E-5
22-23	0.0	4.1441638569235716E-5	0.0	3.3153310855388573E-4	1.036040964230893E-4
24-25	0.0	4.1441638569235716E-5	0.0	4.972996628308286E-4	1.2432491570770715E-4
26-27	0.0	4.1441638569235716E-5	0.0	8.288327713847144E-4	1.2432491570770715E-4
28-29	0.0	4.1441638569235716E-5	0.0	0.0018234320970463716	1.2432491570770715E-4
30-31	0.0	4.1441638569235716E-5	0.0	0.006568499713223861	1.2432491570770715E-4
32-33	0.0	4.1441638569235716E-5	0.0	0.0185658540790176	2.0720819284617857E-4
34-35	0.0	4.1441638569235716E-5	0.0	0.03493530131386571	2.0720819284617857E-4
36-37	0.0	4.1441638569235716E-5	0.0	0.0640480524087538	2.0720819284617857E-4
38-39	0.0	4.1441638569235716E-5	0.0	0.1144203640896598	2.486498314154143E-4
40-41	0.0	4.1441638569235716E-5	0.0	0.17364046560509766	2.486498314154143E-4
42-43	0.0	4.1441638569235716E-5	0.0	0.23909753372520548	2.486498314154143E-4
44-45	0.0	4.1441638569235716E-5	0.0	0.3170078142353686	2.6937065070003216E-4
46-47	0.0	4.1441638569235716E-5	0.0	0.40979564299188737	2.9009146998465E-4
48-49	0.0	4.1441638569235716E-5	0.0	0.5099808042330147	2.9009146998465E-4
50-51	0.0	4.1441638569235716E-5	0.0	0.6195317757907894	2.9009146998465E-4
52-53	0.0	4.1441638569235716E-5	0.0	0.732874657277649	3.108122892692679E-4
54-55	0.0	4.1441638569235716E-5	0.0	0.8570752480696485	3.3153310855388573E-4
56-57	0.0	4.1441638569235716E-5	0.0	0.9985777229643038	3.3153310855388573E-4
58-59	0.0	4.1441638569235716E-5	0.0	1.1625208451442002	3.3153310855388573E-4
60-61	0.0	4.1441638569235716E-5	0.0	1.3469154159580148	3.3153310855388573E-4
62-63	0.0	4.1441638569235716E-5	0.0	1.5364694707736988	3.7297474712312144E-4
64-65	0.0	4.1441638569235716E-5	0.0	1.7372749304609305	3.7297474712312144E-4
66-67	0.0	4.1441638569235716E-5	0.0	1.9639192517960806	3.936955664077393E-4
68-69	0.0	4.1441638569235716E-5	0.0	2.2102690722709024	4.35137204976975E-4
70-71	0.0	8.288327713847143E-5	0.0	2.483369470442166	4.7657884354621076E-4
72-73	0.0	8.288327713847143E-5	0.0	2.7684879437985073	5.801829399693E-4
74-75	0.0	1.2432491570770715E-4	0.0	3.0642154766285734	6.423453978231537E-4
76-77	0.0	1.2432491570770715E-4	0.0	3.3862584499501045	6.630662171077715E-4
78-79	0.0	1.2432491570770715E-4	0.0	3.750157478226563	6.630662171077715E-4
80-81	0.0	1.2432491570770715E-4	0.0	4.133181822702724	7.045078556770072E-4
82-83	0.0	1.2432491570770715E-4	0.0	4.540760338031157	7.459494942462429E-4
84-85	0.0	1.2432491570770715E-4	0.0	4.96901823100564	7.459494942462429E-4
86-87	0.0	1.2432491570770715E-4	0.0	5.409066270153069	7.459494942462429E-4
88-89	0.0	1.2432491570770715E-4	0.0	5.888214495290573	7.459494942462429E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2115	0.0	48.92042	1
GTATCAA	4040	0.0	45.69959	1
TCAACGC	4885	0.0	36.145847	4
CAACGCA	5040	0.0	35.126945	5
ATCAACG	5140	0.0	34.350487	3
TATCAAC	5230	0.0	34.304585	2
AACGCAG	5360	0.0	33.45384	6
GTACATG	9230	0.0	28.230247	1
ACGCAGA	6405	0.0	27.478989	7
TACATGG	9550	0.0	26.987242	2
CGCAGAG	6650	0.0	26.182878	8
ACATGGG	9905	0.0	25.492369	3
GTATAGG	640	0.0	24.472483	1
CATGGGG	8100	0.0	23.264063	4
GCAGAGT	7730	0.0	21.481792	9
ATGGGGG	4655	0.0	20.698477	5
GTATTAG	635	0.0	19.433172	1
AGAGTAC	6575	0.0	19.375103	10-11
GTATTAC	545	0.0	19.158884	1
TACACTG	3345	0.0	18.588196	5
>>END_MODULE