FastQCFastQC Report
Mon 15 Aug 2016
SRR2921705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413032
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.049.774711
GTATCAA39200.045.2397961
TCAACGC48800.035.789124
ATCAACG49700.035.0455363
CAACGCA50200.034.626345
TATCAAC51750.034.0240942
AACGCAG54700.031.7731276
GTACATG88200.028.2462371
TACATGG92050.026.6041242
ACGCAGA65100.026.4043667
ACATGGG95650.025.0074023
CGCAGAG68200.024.7864198
CATGGGG76200.022.2349574
TATAGGG3750.021.5149672
CTATACT9250.021.0360984
TACCGTA1605.0050978E-520.774297
GCAGAGT77850.020.3721089
ATGGGGG46450.019.937845
AGAGTAC66600.018.85817310-11
GTACCGT3701.4370016E-1017.96776
Produced by FastQC (version 0.11.5)