##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921705_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2413032
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.526795334666097	33.0	31.0	34.0	30.0	34.0
2	31.65680852968382	34.0	31.0	34.0	30.0	34.0
3	31.729722191831687	34.0	31.0	34.0	29.0	34.0
4	35.20134710190333	37.0	35.0	37.0	33.0	37.0
5	34.96159396145596	37.0	35.0	37.0	32.0	37.0
6	34.948178888634715	37.0	35.0	37.0	32.0	37.0
7	34.84802356537335	37.0	35.0	37.0	32.0	37.0
8	34.794444499699964	37.0	35.0	37.0	32.0	37.0
9	36.39618745213491	39.0	37.0	39.0	32.0	39.0
10-11	36.29500396182065	39.0	37.0	39.0	32.0	39.0
12-13	36.21928532236622	39.0	37.0	39.0	32.0	39.0
14-15	37.474532662641856	40.0	38.0	41.0	32.0	41.0
16-17	37.44661964698355	40.0	38.0	41.0	32.0	41.0
18-19	37.4096155790723	40.0	38.0	41.0	32.0	41.0
20-21	37.3547132818794	40.0	38.0	41.0	32.0	41.0
22-23	37.27062260260121	40.0	38.0	41.0	31.0	41.0
24-25	37.186745554969846	40.0	38.0	41.0	31.0	41.0
26-27	37.06325858919401	40.0	37.0	41.0	31.0	41.0
28-29	36.947019558795745	40.0	37.0	41.0	30.5	41.0
30-31	36.7948489700924	40.0	37.0	41.0	30.0	41.0
32-33	36.74634899164205	40.0	37.0	41.0	30.0	41.0
34-35	36.887298842286384	40.0	37.0	41.0	30.0	41.0
36-37	36.8920107565917	40.0	37.0	41.0	30.0	41.0
38-39	36.79118801574119	40.0	37.0	41.0	30.0	41.0
40-41	36.714834697592075	40.0	37.0	41.0	30.0	41.0
42-43	36.59732941792733	40.0	37.0	41.0	30.0	41.0
44-45	36.47460559992574	40.0	36.5	41.0	30.0	41.0
46-47	36.314094259835755	40.0	36.0	41.0	29.5	41.0
48-49	36.173334419104265	40.0	36.0	41.0	29.0	41.0
50-51	36.02153846281359	40.0	35.5	41.0	28.0	41.0
52-53	35.85602097278445	39.5	35.0	41.0	28.0	41.0
54-55	35.64428756021471	39.0	35.0	41.0	28.0	41.0
56-57	35.42070494713704	39.0	35.0	41.0	28.0	41.0
58-59	35.179560196466525	39.0	35.0	41.0	27.0	41.0
60-61	34.898912654287216	38.0	35.0	40.0	26.5	41.0
62-63	34.592988199079	37.5	34.5	40.0	26.0	41.0
64-65	34.25972842465413	37.0	34.0	40.0	26.0	41.0
66-67	33.907127423092604	36.5	34.0	39.0	26.0	41.0
68-69	33.537907495632055	36.0	34.0	39.0	25.5	41.0
70-71	33.13890988598576	35.5	34.0	39.0	24.5	40.0
72-73	32.773406030255714	35.0	33.0	37.5	24.0	40.0
74-75	32.373973283404446	35.0	33.0	37.0	23.5	39.0
76-77	29.68100153665596	33.0	29.0	34.5	19.0	36.5
78-79	31.247828458138972	34.5	31.5	36.0	20.0	37.0
80-81	31.423062769163444	35.0	32.0	35.5	21.0	37.0
82-83	31.2479666660036	35.0	32.5	35.0	20.0	37.0
84-85	31.055363542630186	35.0	32.0	35.0	20.0	36.0
86-87	30.854936859519476	35.0	32.0	35.0	19.5	36.0
88-89	30.595100479396876	35.0	32.0	35.0	17.5	36.0
90-91	30.404955052398808	35.0	32.0	35.0	12.5	35.0
92-93	30.253423493762206	34.0	32.0	35.0	10.0	35.0
94-95	30.155527775843836	34.0	32.0	35.0	4.5	35.0
96-97	29.96865126529611	34.0	32.0	35.0	2.0	35.0
98-99	29.785463267789236	34.0	31.5	35.0	2.0	35.0
100-101	28.675477159026485	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39149.0
3	13889.0
4	7742.0
5	5441.0
6	4703.0
7	4773.0
8	4947.0
9	5316.0
10	5966.0
11	6352.0
12	6779.0
13	7215.0
14	7908.0
15	8618.0
16	9212.0
17	10025.0
18	9633.0
19	8547.0
20	8060.0
21	8359.0
22	8649.0
23	9802.0
24	11672.0
25	13938.0
26	16900.0
27	20471.0
28	24623.0
29	29902.0
30	36597.0
31	45725.0
32	58360.0
33	77658.0
34	113370.0
35	168513.0
36	295578.0
37	589493.0
38	632765.0
39	76381.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58008654414548	18.862746498050598	13.78284426703153	25.77432269077239
2	15.024359435530792	20.237560442730047	38.48546307792533	26.25261704381383
3	18.403221160521305	24.666952642060348	28.156971479135194	28.772854718283153
4	13.186477991591847	16.42830855536252	35.18738478575123	35.19782866729441
5	14.468707239638753	37.45497791228502	33.65917393562582	14.417140912450407
6	34.013927113694166	35.02681941209064	16.901720727512036	14.057532746703158
7	29.798788328869374	31.30668441474643	20.299241907791487	18.595285348592704
8	27.14667687788641	35.099534527515594	19.053787931531783	18.700000663066216
9	27.137054714220177	16.023388032220083	18.614778355336796	38.22477889822294
10-11	26.12171357528885	25.303664078111492	27.092281829453885	21.482340517145765
12-13	26.7096749328488	22.35248751100538	27.28894795722132	23.6488895989245
14-15	23.715044728242514	23.925910883354298	24.45044458857684	27.90859979982635
16-17	24.110771036349917	27.194071026424645	24.999238509733505	23.695919427491933
18-19	24.125409060383145	26.589230540661113	26.34544681836859	22.93991358058715
20-21	24.675490173326082	25.56578384632726	26.31438808731285	23.444337893033804
22-23	24.399847246088584	25.378243093801306	26.066982548715174	24.15492711139494
24-25	24.179783010501364	25.67251825513257	25.97025718808796	24.177441546278107
26-27	24.020092359706368	25.836784493051475	26.061342991192966	24.081780156049195
28-29	24.115797204037616	26.081330079721234	25.81969557065582	23.983177145585326
30-31	24.500838196822997	25.72135805086546	25.76342850582116	24.01437524649038
32-33	24.14259949306671	25.774807806963317	25.75358715089275	24.329005549077223
34-35	24.403654705360623	25.869598554364675	25.843466872238665	23.88327986803604
36-37	24.400749793197654	25.899196496212905	25.849257252172876	23.850796458416564
38-39	24.45720755348771	25.84181471352834	25.716557309044102	23.984420423939852
40-41	24.513235772236428	25.663694992373443	25.742934114452044	24.08013512093809
42-43	24.263649183375108	25.63888218283321	25.734848429902264	24.36262020388942
44-45	24.150914541591558	25.71800741480445	25.849738206550647	24.28133983705334
46-47	24.3252013517109	25.712242565299952	25.58138571088737	24.381170372101774
48-49	24.080817366213765	25.78172953424267	25.724431506165185	24.413021593378378
50-51	24.261096707457373	25.75496235341704	25.729288577386853	24.25465236173874
52-53	24.41470303684089	25.69044683047073	25.69504694290411	24.19980318978427
54-55	23.88032268538962	25.438194964355702	26.1197705619103	24.56171178834438
56-57	24.062284162945435	25.60535915182267	25.776174138035003	24.556182547196887
58-59	24.177520801599883	25.648274218450474	25.8789310619955	24.295273917954137
60-61	24.351449244579204	25.593999321552158	25.898699904117954	24.155851529750684
62-63	24.210573001660904	25.552374032192617	25.969382489250957	24.26767047689552
64-65	23.912450895260427	25.366371988133814	26.017453520605905	24.703723595999858
66-67	24.051377211988015	25.66204658781087	25.789618817577725	24.49695738262339
68-69	24.075387052101732	25.627451468185853	25.78809778507517	24.509063694637245
70-71	24.029137889707144	25.349988927419442	25.850204158479727	24.770669024393683
72-73	23.953680175820825	25.66093966637074	25.644523311120814	24.740856846687617
74-75	23.8792383565085	25.642304297172696	25.751407785271734	24.72704956104707
76-77	23.791220419545507	25.24226475567289	25.972293788605604	24.994221036176
78-79	23.778729821775872	25.30901680787639	25.97680253410166	24.93545083624608
80-81	23.926833480999758	25.59573708838027	25.58377310143242	24.893656329187547
82-83	23.9199314038845	25.596262448538926	25.691396655131037	24.792409492445536
84-85	23.812328485686038	25.555453971191916	25.706482968966803	24.925734574155236
86-87	23.622598928161615	25.720803642409233	25.69494698244277	24.961650446986383
88-89	23.630925251512547	25.434695274263863	25.757623936707812	25.176755537515778
90-91	23.66348116340842	25.298251229377016	25.811388832310065	25.2268787749045
92-93	23.686270639816694	25.38370923348545	25.777390594770043	25.15262953192781
94-95	23.513624577019847	25.278448336065644	25.8483071779336	25.359619908980907
96-97	23.253087553172396	25.282954037247514	25.900485395646566	25.563473013933518
98-99	23.167424433622557	25.10423698855911	25.872861748247317	25.85547682957102
100-101	23.219487390733715	25.16496914456407	25.87762175889453	25.737921705807686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	22.0
2	35.5
3	50.5
4	44.0
5	54.0
6	89.0
7	217.0
8	337.5
9	358.5
10	322.0
11	275.0
12	267.0
13	278.5
14	320.0
15	381.5
16	416.5
17	444.0
18	511.0
19	575.5
20	639.5
21	710.5
22	830.0
23	1009.5
24	1207.5
25	1522.5
26	1944.5
27	2499.0
28	3237.5
29	4314.0
30	6174.0
31	9158.5
32	13423.0
33	18324.0
34	23536.5
35	29374.5
36	36987.5
37	44814.5
38	52031.0
39	59004.5
40	64518.0
41	69524.5
42	75375.0
43	82330.5
44	89524.5
45	96710.5
46	100280.0
47	103545.5
48	108098.0
49	110362.0
50	110643.0
51	109735.0
52	108587.5
53	106759.0
54	104662.0
55	100055.5
56	92179.0
57	82823.5
58	74272.0
59	64922.0
60	53650.5
61	43839.5
62	35958.5
63	27774.0
64	19990.0
65	14690.0
66	11280.5
67	8749.0
68	6845.0
69	5326.0
70	3997.0
71	2875.5
72	2030.5
73	1540.0
74	1200.0
75	929.5
76	664.5
77	392.5
78	241.5
79	148.0
80	93.5
81	53.0
82	29.5
83	20.5
84	13.0
85	8.0
86	5.5
87	4.5
88	3.0
89	4.5
90	4.5
91	2.0
92	1.5
93	1.0
94	1.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1694134184710356
2	1.6576655427694286E-4
3	0.009821668340908864
4	0.005636062845416057
5	0.02507219133438761
6	0.0016991071813386646
7	4.1441638569235716E-5
8	0.0
9	1.2432491570770715E-4
10-11	0.04962636218665978
12-13	3.108122892692679E-4
14-15	0.005263088098292935
16-17	2.0720819284617858E-5
18-19	0.0013468532535001608
20-21	3.3153310855388573E-4
22-23	2.0720819284617858E-5
24-25	4.972996628308286E-4
26-27	0.003108122892692679
28-29	0.005138763182585228
30-31	0.017094675909809735
32-33	0.011955912727224504
34-35	0.0104432929194474
36-37	0.004144163856923571
38-39	4.1441638569235716E-5
40-41	0.0034189351819619465
42-43	0.008474815087408704
44-45	0.00648561643608539
46-47	0.003729747471231214
48-49	0.008661302460970265
50-51	0.002652264868431086
52-53	0.0019684778320386963
54-55	0.0038747932062235396
56-57	0.007770307231731697
58-59	0.014794664969217152
60-61	0.007107241014623925
62-63	0.020347844537494737
64-65	0.04013622695430479
66-67	0.06038046739537644
68-69	0.050227265945913695
70-71	0.06920753641062365
72-73	0.0332983565903809
74-75	0.026667694419303185
76-77	0.23180380533702
78-79	0.08615716658544105
80-81	0.06748770841000036
82-83	0.026957785889287832
84-85	0.05153267756084461
86-87	0.06883456166350052
88-89	0.2999338591448435
90-91	0.30426451037532865
92-93	0.05428854652569879
94-95	0.010008155714470425
96-97	0.014960431523494095
98-99	8.702744099539501E-4
100-101	2.072081928461786E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2413032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.82915581128533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61394002334785	47.820196066192665
2	11.95955752415335	14.549795761415082
3	3.894263370527374	7.106542600079726
4	1.8169154839701633	4.420857402814319
5	0.9945764492477545	3.024962289876329
6	0.5997858161324492	2.1890678917751814
7	0.4159790084333597	1.7712556342751768
8	0.29242169626210723	1.423021193962246
9	0.21063903871375414	1.153169541527248
>10	1.1296198749656476	11.835129221872567
>50	0.05148936819677129	2.0930025636935152
>100	0.019651182605910738	2.080845574461346
>500	0.0010245559795724786	0.4338918579690888
>1k	1.366074639425119E-4	0.09826240008562304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.288327713847143E-5	0.0
3	0.0	0.0	0.0	8.288327713847143E-5	0.0
4	0.0	0.0	0.0	1.2432491570770715E-4	0.0
5	0.0	0.0	0.0	1.2432491570770715E-4	0.0
6	0.0	0.0	0.0	1.2432491570770715E-4	4.1441638569235716E-5
7	0.0	0.0	0.0	1.2432491570770715E-4	4.1441638569235716E-5
8	0.0	0.0	0.0	1.2432491570770715E-4	4.1441638569235716E-5
9	0.0	0.0	0.0	1.2432491570770715E-4	4.1441638569235716E-5
10-11	0.0	0.0	0.0	1.2432491570770715E-4	8.288327713847143E-5
12-13	0.0	0.0	0.0	1.2432491570770715E-4	2.0720819284617857E-4
14-15	0.0	0.0	0.0	1.2432491570770715E-4	2.0720819284617857E-4
16-17	0.0	0.0	0.0	2.2792901213079645E-4	2.0720819284617857E-4
18-19	0.0	0.0	0.0	3.522539278385036E-4	2.0720819284617857E-4
20-21	0.0	0.0	0.0	3.936955664077393E-4	2.486498314154143E-4
22-23	0.0	0.0	0.0	4.558580242615929E-4	2.9009146998465E-4
24-25	0.0	0.0	0.0	6.216245785385357E-4	3.7297474712312144E-4
26-27	0.0	0.0	0.0	9.117160485231858E-4	4.1441638569235715E-4
28-29	0.0	0.0	0.0	0.0019477570127540787	4.1441638569235715E-4
30-31	0.0	0.0	0.0	0.006837870363923893	4.1441638569235715E-4
32-33	0.0	0.0	0.0	0.01931180357326384	4.1441638569235715E-4
34-35	0.0	0.0	0.0	0.036095667193804304	4.558580242615929E-4
36-37	0.0	0.0	0.0	0.06535346402368472	4.558580242615929E-4
38-39	0.0	0.0	0.0	0.11576721734315998	4.972996628308286E-4
40-41	0.0	0.0	0.0	0.1760026390035441	4.972996628308286E-4
42-43	0.0	0.0	0.0	0.24152186958150576	5.180204821154464E-4
44-45	0.0	0.0	0.0	0.32003305385092284	5.594621206846822E-4
46-47	0.0	0.0	0.0	0.41311097407742625	5.801829399693E-4
48-49	0.0	0.0	0.0	0.5134204602342614	6.216245785385357E-4
50-51	0.0	0.0	0.0	0.6232200816234512	6.216245785385357E-4
52-53	0.0	0.0	0.0	0.7364179173753187	6.216245785385357E-4
54-55	0.0	0.0	0.0	0.8613644576615644	6.423453978231537E-4
56-57	0.0	0.0	0.0	1.0036750445083198	6.630662171077715E-4
58-59	0.0	0.0	0.0	1.1673695168568008	6.837870363923893E-4
60-61	0.0	0.0	0.0	1.353235265839823	7.045078556770072E-4
62-63	0.0	0.0	0.0	1.5435352701497536	7.459494942462429E-4
64-65	0.0	0.0	0.0	1.743636221981308	7.666703135308607E-4
66-67	0.0	0.0	0.0	1.9706742388828662	7.873911328154786E-4
68-69	0.0	0.0	0.0	2.2176871255747956	7.873911328154786E-4
70-71	4.1441638569235716E-5	0.0	0.0	2.491699239794582	7.873911328154786E-4
72-73	4.1441638569235716E-5	0.0	0.0	2.7767762715123547	8.909952292385679E-4
74-75	4.1441638569235716E-5	0.0	0.0	3.072876779089544	9.117160485231858E-4
76-77	4.1441638569235716E-5	0.0	0.0	3.3959557933753057	9.324368678078037E-4
78-79	4.1441638569235716E-5	0.0	0.0	3.7600413090253255	9.531576870924215E-4
80-81	6.216245785385358E-5	0.0	0.0	4.145117014610664	9.738785063770393E-4
82-83	8.288327713847143E-5	0.0	0.0	4.554373916301151	0.0010360409642308928
84-85	8.288327713847143E-5	0.0	0.0	4.985056145131933	0.0010360409642308928
86-87	8.288327713847143E-5	0.0	0.0	5.429517718786986	0.0010567617835155107
88-89	8.288327713847143E-5	0.0	0.0	5.912147041564306	0.0010774826028001287
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2025	0.0	49.77471	1
GTATCAA	3920	0.0	45.239796	1
TCAACGC	4880	0.0	35.78912	4
ATCAACG	4970	0.0	35.045536	3
CAACGCA	5020	0.0	34.62634	5
TATCAAC	5175	0.0	34.024094	2
AACGCAG	5470	0.0	31.773127	6
GTACATG	8820	0.0	28.246237	1
TACATGG	9205	0.0	26.604124	2
ACGCAGA	6510	0.0	26.404366	7
ACATGGG	9565	0.0	25.007402	3
CGCAGAG	6820	0.0	24.786419	8
CATGGGG	7620	0.0	22.234957	4
TATAGGG	375	0.0	21.514967	2
CTATACT	925	0.0	21.036098	4
TACCGTA	160	5.0050978E-5	20.77429	7
GCAGAGT	7785	0.0	20.372108	9
ATGGGGG	4645	0.0	19.93784	5
AGAGTAC	6660	0.0	18.858173	10-11
GTACCGT	370	1.4370016E-10	17.9677	6
>>END_MODULE