FastQCFastQC Report
Mon 15 Aug 2016
SRR2921704_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921704_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2978522
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37600.044.0587461
GGTATCA19550.042.732711
TCAACGC43750.036.5652284
CAACGCA45400.034.917365
ATCAACG46350.034.206283
TATCAAC48450.033.5074582
AACGCAG51100.031.2821886
GTACATG85600.027.5044171
ACGCAGA60900.025.5502857
TACATGG90600.025.4634462
ACATGGG92750.024.5149353
CGCAGAG64450.023.9245288
CATGGGG79450.021.8079784
GTATAGC10700.021.7373621
TAGCCCT22600.019.1139034
GCAGAGT76450.018.928349
GTATATA8800.018.339641
GTGTAGC20600.018.203481
ATGGGGG45450.017.8571835
AGAGTAC63200.017.64187210-11
Produced by FastQC (version 0.11.5)