##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921704_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2978522
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.424500809461875	31.0	31.0	34.0	26.0	34.0
2	30.478106926858356	31.0	31.0	34.0	26.0	34.0
3	30.541098235970725	33.0	31.0	34.0	26.0	34.0
4	33.99170729643763	37.0	35.0	37.0	30.0	37.0
5	33.98790071048661	37.0	35.0	37.0	30.0	37.0
6	34.029242355772425	37.0	35.0	37.0	30.0	37.0
7	33.95196275199579	37.0	35.0	37.0	30.0	37.0
8	33.91987200363133	37.0	35.0	37.0	30.0	37.0
9	35.47804347256794	39.0	37.0	39.0	30.0	39.0
10-11	35.37763696222489	39.0	36.0	39.0	30.0	39.0
12-13	35.26998239395243	39.0	35.0	39.0	29.0	39.0
14-15	36.41615740961457	40.0	37.0	41.0	29.0	41.0
16-17	36.357345522376534	40.0	37.0	41.0	28.0	41.0
18-19	36.32017205177601	40.0	37.0	41.0	28.0	41.0
20-21	36.24177578678284	40.0	37.0	41.0	27.0	41.0
22-23	36.142634165535796	40.0	36.5	41.0	27.0	41.0
24-25	36.03782211445811	40.0	36.0	41.0	27.0	41.0
26-27	35.89363315093862	40.0	36.0	41.0	27.0	41.0
28-29	35.75484149521138	40.0	36.0	41.0	27.0	41.0
30-31	35.612061116218044	40.0	36.0	41.0	26.0	41.0
32-33	35.44387703028549	39.5	35.0	41.0	25.0	41.0
34-35	35.279978291246465	39.0	35.0	41.0	25.0	41.0
36-37	35.25497159329359	39.0	35.0	41.0	25.0	41.0
38-39	35.446369038066535	40.0	35.0	41.0	25.0	41.0
40-41	35.51568412118493	40.0	35.0	41.0	25.0	41.0
42-43	35.366006529412914	40.0	35.0	41.0	24.5	41.0
44-45	35.25266877330434	40.0	35.0	41.0	24.0	41.0
46-47	35.09849499181138	40.0	35.0	41.0	23.5	41.0
48-49	34.94000917233447	39.5	35.0	41.0	23.0	41.0
50-51	33.24963992208216	37.0	32.5	39.5	19.0	40.0
52-53	33.36986079001599	37.5	33.5	39.5	20.0	40.0
54-55	34.28687046797036	39.0	34.0	40.5	21.5	41.0
56-57	34.308630253528435	39.0	34.0	41.0	21.0	41.0
58-59	34.124037358125946	38.5	34.0	41.0	20.0	41.0
60-61	33.87251714105184	38.0	34.0	40.0	19.5	41.0
62-63	33.57891430716309	37.0	34.0	40.0	18.0	41.0
64-65	33.28896815266095	37.0	33.5	40.0	17.5	41.0
66-67	32.94750836152964	36.0	33.0	39.5	13.5	41.0
68-69	32.58354529528404	36.0	33.0	39.0	12.0	41.0
70-71	32.173799287028935	35.0	32.5	39.0	8.0	40.5
72-73	31.809386165353153	35.0	32.0	37.5	7.0	40.0
74-75	31.41024440981131	35.0	32.0	37.0	2.0	39.0
76-77	31.030848017909555	35.0	32.0	36.5	2.0	39.0
78-79	30.693916479381386	35.0	32.0	36.0	2.0	38.0
80-81	30.387195058488743	35.0	31.0	36.0	2.0	37.0
82-83	30.089018647503693	35.0	31.0	35.0	2.0	37.0
84-85	29.83817947290636	35.0	31.0	35.0	2.0	36.0
86-87	29.62181209338054	35.0	31.0	35.0	2.0	36.0
88-89	29.420111048365598	35.0	31.0	35.0	2.0	36.0
90-91	29.21645114590391	34.5	31.0	35.0	2.0	35.0
92-93	29.045108110666966	34.0	30.0	35.0	2.0	35.0
94-95	28.83273264390862	34.0	30.0	35.0	2.0	35.0
96-97	28.614618928448404	34.0	30.0	35.0	2.0	35.0
98-99	28.368363067319965	34.0	30.0	35.0	2.0	35.0
100-101	27.31619390422498	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100023.0
3	17198.0
4	11694.0
5	9005.0
6	8058.0
7	8323.0
8	8819.0
9	9628.0
10	10352.0
11	11129.0
12	10794.0
13	11038.0
14	11390.0
15	12240.0
16	11927.0
17	12545.0
18	12214.0
19	11878.0
20	11927.0
21	12533.0
22	13860.0
23	15867.0
24	18678.0
25	21947.0
26	26224.0
27	31008.0
28	36841.0
29	43725.0
30	52514.0
31	64110.0
32	80013.0
33	103262.0
34	144829.0
35	210578.0
36	352238.0
37	645837.0
38	697318.0
39	106951.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.59547208220729	17.358496270405276	12.778779017523467	25.267252629863968
2	16.166164736731226	19.308448853378778	39.08850912529119	25.436877284598808
3	19.69450378283649	23.345298820228077	28.402150562200934	28.558046834734498
4	13.53763098009583	15.507284017026446	36.21758613263197	34.73749887024575
5	14.90934979057203	36.70477593878058	33.8325201137869	14.553354156860493
6	34.0820385950209	34.428589272475264	17.289524438857192	14.19984769364665
7	29.979181357241192	30.874618045062284	20.44088512810181	18.70531546959471
8	27.07675796708564	34.085825550349554	19.70673992459967	19.13067655796514
9	27.092000126245587	15.802287328233819	18.740485414270182	38.36522713125042
10-11	26.02381266976941	24.86729465782194	27.53951695372971	21.569375718678945
12-13	26.808571013599682	22.102976079037486	27.16522364038884	23.923229266973994
14-15	23.865380229662993	23.596329449414053	24.5161166808125	28.02217364011045
16-17	24.270929114945993	26.58174412817435	25.180374477699857	23.966952279179797
18-19	24.147769721124902	26.180094452549596	26.25780878814995	23.41432703817555
20-21	24.87994984422023	25.374405373948676	26.107567626015726	23.638077155815367
22-23	24.552894078505112	25.264236547442337	25.813272895784696	24.36959647826786
24-25	24.377723974197117	25.37456796765874	25.886222555387967	24.361485502756175
26-27	24.277217116524913	25.572281646438366	25.862152049316922	24.2883491877198
28-29	24.39456559167261	25.79356158636264	25.613400705768623	24.19847211619613
30-31	24.700080938734867	25.561647832727285	25.51893716242999	24.21933406610786
32-33	24.36554418837033	25.541163114985554	25.537117129761295	24.55617556688282
34-35	24.673327370328142	25.5940509622061	25.57598735413133	24.156634313334436
36-37	24.675352144949642	25.514349269841347	25.534124347636467	24.276174237572548
38-39	24.545653859235205	25.62267471707134	25.574158751625454	24.257512672068
40-41	24.774239100911437	25.37165731682873	25.61710105446296	24.237002527796868
42-43	24.5440091054308	25.322763055360692	25.54164878095896	24.59157905824955
44-45	24.46643515637038	25.458994619101418	25.641441868195237	24.43312835633297
46-47	24.600147838559216	25.38232282840667	25.431310753354207	24.5862185796799
48-49	24.404474994670984	25.441324200567543	25.57196292720252	24.582237877558956
50-51	24.46520740172268	25.502140314316502	25.600113988601812	24.432538295359002
52-53	24.65644991912807	25.40354369456353	25.58377689239811	24.356229493910295
54-55	24.264874360345225	25.19658586102605	25.896795240823778	24.641744537804946
56-57	24.33529334474874	25.344813344116172	25.71390742247704	24.60598588865805
58-59	24.449086682008932	25.315967341769003	25.73264143953035	24.502304536691717
60-61	24.500026332336294	25.234505018539476	25.835000066461806	24.43046858266242
62-63	24.399655994557286	25.272892815252522	25.884222518763167	24.443228671427022
64-65	24.244105040488577	25.07467010399812	25.869452062583925	24.81177279292938
66-67	24.2655384957538	25.31897616067149	25.717041317050267	24.698444026524445
68-69	24.3650859336835	25.268765652903554	25.73630047115156	24.629847942261392
70-71	24.276717415047024	25.0282796724621	25.82032916950024	24.874673742990634
72-73	24.273631780535794	25.11491549648339	25.753822778132175	24.857629944848636
74-75	24.190294604408887	25.18984948303004	25.808931564017907	24.81092434854316
76-77	24.120553481023144	24.904459273720587	25.95208926318878	25.022897982067494
78-79	24.098928467297213	24.971949427707756	25.96768796154737	24.961434143447658
80-81	24.176087481066105	25.10007494473373	25.80747313751231	24.916364436687854
82-83	24.144037214229673	25.142757916192938	25.8988444252102	24.814360444367193
84-85	24.156028533466184	25.064133334746646	25.895464893697746	24.88437323808942
86-87	23.96209824467517	25.18686245257442	25.92397939822002	24.927059904530388
88-89	24.020939264924714	24.945214397940603	25.95986915735933	25.07397717977536
90-91	23.996804583843662	24.90460703348321	26.041059784977506	25.057528597695626
92-93	24.00719284874942	24.850952508923328	26.138341851815266	25.003512790511984
94-95	23.935882788794434	24.76499684385027	26.15282765268453	25.14629271467077
96-97	23.848106855804378	24.71432605722668	26.18834604472566	25.249221042243292
98-99	23.71649695323583	24.543422910387605	26.24883674358884	25.491243392787727
100-101	23.854540387328175	24.532837602815782	26.345545847163	25.267076162693048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	18.0
2	38.0
3	54.5
4	56.0
5	63.0
6	93.0
7	198.5
8	318.0
9	387.0
10	362.0
11	329.5
12	377.5
13	450.0
14	531.5
15	639.0
16	786.0
17	863.0
18	907.5
19	994.0
20	1083.5
21	1176.5
22	1251.5
23	1350.5
24	1493.0
25	1669.5
26	1957.0
27	2307.5
28	2734.5
29	3437.0
30	4484.5
31	6301.0
32	9694.5
33	13992.5
34	19729.5
35	27631.5
36	37641.5
37	48249.5
38	58668.0
39	69524.5
40	78518.0
41	86712.0
42	96287.5
43	105911.0
44	114897.0
45	123796.0
46	129612.0
47	134304.5
48	139664.5
49	142183.5
50	141673.5
51	139688.0
52	137921.5
53	135367.0
54	131828.5
55	125162.5
56	114533.5
57	102684.5
58	92426.5
59	80871.0
60	66466.5
61	54474.0
62	44676.5
63	34386.0
64	25138.5
65	18655.0
66	14087.0
67	10959.5
68	8404.0
69	6392.5
70	4975.0
71	3658.5
72	2598.0
73	1949.0
74	1494.0
75	1113.0
76	823.0
77	573.5
78	398.0
79	271.5
80	198.0
81	155.5
82	129.5
83	111.5
84	94.0
85	71.0
86	49.5
87	47.0
88	46.0
89	43.0
90	37.0
91	26.0
92	21.0
93	23.5
94	21.0
95	16.0
96	12.0
97	8.0
98	9.5
99	10.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.055631618635014285
2	0.07866317589730745
3	0.07332495781464766
4	0.07423144767774084
5	0.06986686685544038
6	0.010542141370787256
7	0.014168100823159942
8	0.01849910794682732
9	0.0067147397266160876
10-11	0.013479840001181794
12-13	0.02331693370067436
14-15	0.04038915945559576
16-17	0.027799022468190597
18-19	0.010307125480355693
20-21	0.020144219179848263
22-23	0.03772005041426587
24-25	0.03441304109890744
26-27	0.021470380275854937
28-29	0.011767581370894691
30-31	0.01153256548046313
32-33	0.008762735343233994
34-35	0.005304644384026709
36-37	8.561293151435511E-4
38-39	0.004062417534602732
40-41	0.001309374246690137
42-43	0.23865863673325224
44-45	0.20667968878524315
46-47	0.21356229700502466
48-49	0.2210995923481512
50-51	0.21134643289524133
52-53	0.21953841536171298
54-55	0.21524098193667868
56-57	0.21874943344383557
58-59	0.22746180823911993
60-61	0.23133957043124073
62-63	0.21742327234782888
64-65	0.21651678248473571
66-67	0.21472058960786594
68-69	0.20916414248409113
70-71	0.20078750467513753
72-73	0.21279010193646378
74-75	0.21148072768977366
76-77	0.204749201113841
78-79	0.223516898649733
80-81	0.23498231673292996
82-83	0.23402546632188717
84-85	0.22774718467750113
86-87	0.24910005700814028
88-89	0.24060591125397093
90-91	0.2481432065970975
92-93	0.24298964385691965
94-95	0.24638058741886076
96-97	0.2539850301592535
98-99	0.24633022687091116
100-101	0.2535821457756565
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2978522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.24487929201919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52179549392864	53.188797066700474
2	10.76690778754408	14.049711978932297
3	3.1660184858313345	6.196994818331003
4	1.4647042839150803	3.822578168101713
5	0.7955159662522141	2.5951671596499857
6	0.5217110670749616	2.0423385357969837
7	0.33288566699643507	1.5203359612857992
8	0.24905441663726505	1.299962028051409
9	0.19445737311162922	1.1418613052500248
>10	0.93225401088131	10.556753071193237
>50	0.042025799400325754	1.8455099092584217
>100	0.011793536068512968	1.3033743751586107
>500	7.215042952194205E-4	0.30292415421330954
>1k	1.5460806306459582E-4	0.1336914680766548
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3573698633080436E-5
2	0.0	0.0	0.0	0.0	3.3573698633080436E-5
3	0.0	0.0	0.0	0.0	3.3573698633080436E-5
4	0.0	0.0	0.0	0.0	6.714739726616087E-5
5	0.0	0.0	0.0	0.0	6.714739726616087E-5
6	0.0	0.0	0.0	0.0	6.714739726616087E-5
7	0.0	0.0	0.0	6.714739726616087E-5	6.714739726616087E-5
8	0.0	0.0	0.0	6.714739726616087E-5	1.0072109589924131E-4
9	0.0	0.0	0.0	1.0072109589924131E-4	1.0072109589924131E-4
10-11	0.0	0.0	0.0	1.0072109589924131E-4	1.0072109589924131E-4
12-13	0.0	0.0	0.0	1.3429479453232174E-4	2.0144219179848261E-4
14-15	0.0	0.0	0.0	1.6786849316540218E-4	3.021632876977239E-4
16-17	0.0	0.0	0.0	1.6786849316540218E-4	3.693106849638848E-4
18-19	0.0	1.6786849316540218E-5	0.0	2.3501589043156305E-4	4.1967123291350547E-4
20-21	0.0	3.3573698633080436E-5	0.0	2.5180273974810324E-4	4.5324493154658585E-4
22-23	0.0	3.3573698633080436E-5	0.0	2.8537643838118373E-4	5.036054794962065E-4
24-25	0.0	3.3573698633080436E-5	0.0	3.3573698633080436E-4	5.37179178129287E-4
26-27	0.0	3.3573698633080436E-5	0.0	5.875397260789076E-4	5.707528767623674E-4
28-29	0.0	3.3573698633080436E-5	0.0	0.0015779638357547804	5.707528767623674E-4
30-31	0.0	3.3573698633080436E-5	0.0	0.007587655891076178	5.707528767623674E-4
32-33	0.0	3.3573698633080436E-5	0.0	0.02393804712538635	6.043265753954477E-4
34-35	0.0	6.714739726616087E-5	0.0	0.0476242915110246	6.714739726616087E-4
36-37	0.0	6.714739726616087E-5	0.0	0.08421962302108227	6.714739726616087E-4
38-39	0.0	6.714739726616087E-5	0.0	0.14950368001310715	7.218345206112293E-4
40-41	0.0	6.714739726616087E-5	0.0	0.22373512769084802	7.386213699277696E-4
42-43	0.0	6.714739726616087E-5	0.0	0.30503383893085223	7.386213699277696E-4
44-45	0.0	6.714739726616087E-5	0.0	0.4025486466106344	7.7219506856085E-4
46-47	0.0	6.714739726616087E-5	0.0	0.5175553512782514	8.057687671939305E-4
48-49	0.0	6.714739726616087E-5	0.0	0.6408043989602897	8.729161644600912E-4
50-51	0.0	6.714739726616087E-5	0.0	0.7732358532184755	9.064898630931717E-4
52-53	0.0	6.714739726616087E-5	0.0	0.9105019200798248	9.400635617262522E-4
54-55	0.0	1.0072109589924131E-4	0.0	1.0653270313262753	9.736372603593326E-4
56-57	0.0	1.0072109589924131E-4	0.0	1.2457520877804495	9.904241096758727E-4
58-59	0.0	1.0072109589924131E-4	0.0	1.442963993551164	0.001007210958992413
60-61	0.0	1.0072109589924131E-4	0.0	1.65545193219993	0.001007210958992413
62-63	0.0	1.0072109589924131E-4	0.0	1.869517834684451	0.0010407846576254935
64-65	0.0	1.0072109589924131E-4	0.0	2.1023514347048637	0.0010407846576254935
66-67	0.0	1.0072109589924131E-4	0.0	2.380039496099072	0.0010407846576254935
68-69	0.0	1.0072109589924131E-4	0.0	2.6765624024264385	0.0010407846576254935
70-71	0.0	1.3429479453232174E-4	0.0	2.9990545646464923	0.0010407846576254935
72-73	0.0	1.3429479453232174E-4	0.0	3.3306619860454276	0.0011750794521578152
74-75	0.0	1.3429479453232174E-4	0.0	3.6766557373086384	0.0011918663014743554
76-77	0.0	1.3429479453232174E-4	0.0	4.064666972411149	0.0012086531507908957
78-79	0.0	1.3429479453232174E-4	0.0	4.492295171900695	0.0012086531507908957
80-81	0.0	1.3429479453232174E-4	0.0	4.942182733583972	0.001242226849423976
82-83	0.0	1.3429479453232174E-4	0.0	5.415420802666557	0.0013429479453232174
84-85	0.0	1.3429479453232174E-4	0.0	5.91200937914845	0.0013429479453232174
86-87	0.0	1.5108164384886196E-4	0.0	6.44359853645533	0.0013429479453232174
88-89	0.0	2.0144219179848261E-4	0.0	7.00861031075144	0.0013429479453232174
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3760	0.0	44.058746	1
GGTATCA	1955	0.0	42.73271	1
TCAACGC	4375	0.0	36.565228	4
CAACGCA	4540	0.0	34.91736	5
ATCAACG	4635	0.0	34.20628	3
TATCAAC	4845	0.0	33.507458	2
AACGCAG	5110	0.0	31.282188	6
GTACATG	8560	0.0	27.504417	1
ACGCAGA	6090	0.0	25.550285	7
TACATGG	9060	0.0	25.463446	2
ACATGGG	9275	0.0	24.514935	3
CGCAGAG	6445	0.0	23.924528	8
CATGGGG	7945	0.0	21.807978	4
GTATAGC	1070	0.0	21.737362	1
TAGCCCT	2260	0.0	19.113903	4
GCAGAGT	7645	0.0	18.92834	9
GTATATA	880	0.0	18.33964	1
GTGTAGC	2060	0.0	18.20348	1
ATGGGGG	4545	0.0	17.857183	5
AGAGTAC	6320	0.0	17.641872	10-11
>>END_MODULE