FastQCFastQC Report
Mon 15 Aug 2016
SRR2921704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2978522
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.048.578611
GTATCAA36450.042.6435851
TCAACGC44500.034.2384034
CAACGCA45200.033.6285675
ATCAACG46050.033.2932363
TATCAAC46550.032.731142
AACGCAG49500.030.7975656
GTACATG85750.027.3839651
TACATGG90300.025.4409562
ACGCAGA63600.023.446317
ACATGGG94050.023.3166563
CGCAGAG65850.022.4288278
CATGGGG81000.022.0328854
ATGGGGG45550.018.6664375
GCAGAGT77400.018.2845619
AGAGTAC64500.017.67098610-11
GTATAAA10050.017.5000461
GTATAGC10400.017.368161
CGTACAC4507.2759576E-1216.8768183
GAGTACA64850.016.0523131
Produced by FastQC (version 0.11.5)