##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2978522
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409604159378375	33.0	31.0	34.0	29.0	34.0
2	31.5375595681348	34.0	31.0	34.0	29.0	34.0
3	31.613003026333196	34.0	31.0	34.0	28.0	34.0
4	35.086595297936356	37.0	35.0	37.0	32.0	37.0
5	34.835091699843076	37.0	35.0	37.0	32.0	37.0
6	34.81047009221352	37.0	35.0	37.0	32.0	37.0
7	34.701499267086156	37.0	35.0	37.0	32.0	37.0
8	34.6428278857769	37.0	35.0	37.0	32.0	37.0
9	36.24063411316082	39.0	37.0	39.0	32.0	39.0
10-11	36.131952021841705	39.0	37.0	39.0	32.0	39.0
12-13	36.05666417773648	39.0	37.0	39.0	32.0	39.0
14-15	37.30458915529246	40.0	38.0	41.0	32.0	41.0
16-17	37.27263756990884	40.0	38.0	41.0	31.0	41.0
18-19	37.22786016017341	40.0	38.0	41.0	31.0	41.0
20-21	37.168537449110666	40.0	38.0	41.0	31.0	41.0
22-23	37.08390386238544	40.0	38.0	41.0	31.0	41.0
24-25	37.002578795792004	40.0	37.0	41.0	31.0	41.0
26-27	36.87394922716703	40.0	37.0	41.0	30.0	41.0
28-29	36.7573242702253	40.0	37.0	41.0	30.0	41.0
30-31	36.61126944840427	40.0	37.0	41.0	30.0	41.0
32-33	36.56681518551819	40.0	37.0	41.0	30.0	41.0
34-35	36.707756900905885	40.0	37.0	41.0	30.0	41.0
36-37	36.70806510745933	40.0	37.0	41.0	30.0	41.0
38-39	36.60714542313268	40.0	37.0	41.0	30.0	41.0
40-41	36.536095754874395	40.0	37.0	41.0	30.0	41.0
42-43	36.42348369426178	40.0	37.0	41.0	30.0	41.0
44-45	36.30106912085927	40.0	36.0	41.0	29.0	41.0
46-47	36.150674227015955	40.0	36.0	41.0	28.5	41.0
48-49	36.008758202893915	40.0	36.0	41.0	28.0	41.0
50-51	35.85432069999818	40.0	35.0	41.0	28.0	41.0
52-53	35.68277538322698	39.0	35.0	41.0	28.0	41.0
54-55	35.46915013553702	39.0	35.0	41.0	27.0	41.0
56-57	35.24454628839404	39.0	35.0	41.0	27.0	41.0
58-59	35.0059856868608	38.5	35.0	40.5	26.0	41.0
60-61	34.72525803066085	38.0	34.5	40.0	26.0	41.0
62-63	34.421336824102696	37.5	34.0	40.0	26.0	41.0
64-65	34.084056790582714	37.0	34.0	40.0	26.0	41.0
66-67	33.729233156579	36.5	34.0	39.0	25.0	41.0
68-69	33.361199279374134	36.0	34.0	39.0	24.0	41.0
70-71	32.95918378309779	35.5	33.0	38.5	23.0	40.0
72-73	32.590509991196974	35.0	33.0	37.5	22.5	39.5
74-75	32.18422392045451	35.0	33.0	37.0	21.5	39.0
76-77	29.48123398114904	33.0	29.0	34.5	17.0	36.5
78-79	31.048389436102873	34.5	31.5	36.0	19.0	37.0
80-81	31.22382174783332	35.0	32.0	35.5	20.0	37.0
82-83	31.048282168135742	35.0	32.0	35.0	19.0	37.0
84-85	30.851608616622606	35.0	32.0	35.0	18.0	36.0
86-87	30.647331797448533	35.0	32.0	35.0	17.5	36.0
88-89	30.38546550940366	35.0	32.0	35.0	10.5	36.0
90-91	30.19349193996217	34.5	31.5	35.0	6.0	35.0
92-93	30.041667142294063	34.0	31.0	35.0	4.0	35.0
94-95	29.936835450602683	34.0	31.0	35.0	2.0	35.0
96-97	29.74226747359932	34.0	31.0	35.0	2.0	35.0
98-99	29.54471529839296	34.0	31.0	35.0	2.0	35.0
100-101	28.431537017352902	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54494.0
3	18293.0
4	10014.0
5	7200.0
6	6071.0
7	6180.0
8	6429.0
9	6955.0
10	7736.0
11	8393.0
12	8797.0
13	9452.0
14	9992.0
15	10779.0
16	11247.0
17	12032.0
18	11538.0
19	10661.0
20	10561.0
21	10290.0
22	11080.0
23	12631.0
24	15066.0
25	17918.0
26	21661.0
27	26241.0
28	31433.0
29	38508.0
30	46561.0
31	58103.0
32	74112.0
33	97717.0
34	140821.0
35	210649.0
36	370585.0
37	723660.0
38	762313.0
39	82345.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45364121593922	18.858566301806878	13.364885684104536	26.32290679814937
2	15.320298443622486	20.64254080635376	37.46627098903851	26.57088976098524
3	18.308969121353886	24.391722939339004	28.322846598641398	28.97646134066571
4	13.276422150033994	16.702979023444385	35.1783972534898	34.84220157303182
5	14.6447042893666	37.04258806706654	33.922624362817814	14.390083280749048
6	34.08843449938609	35.017003793580095	16.979116339925458	13.915445367108362
7	29.72595132753762	31.117111104097937	20.49560150974208	18.661336058622364
8	27.03790672017867	34.53125409179452	19.534755828561952	18.896083359464864
9	26.867849424495866	15.700285947479268	18.696707994812186	38.73515663321268
10-11	25.901367696615623	25.12615150407662	27.508837827379196	21.46364297192856
12-13	26.673146411170006	22.28298521493531	27.23075696341714	23.813111410477543
14-15	23.75654342591974	23.799066671009086	24.471900914963246	27.97248898810793
16-17	24.21053126349243	26.82216213276249	25.109819568228808	23.85748703551627
18-19	24.096516603144163	26.42751048188467	26.255929214711436	23.22004370025973
20-21	24.773779507203265	25.52133449274973	26.074530553867536	23.630355446179465
22-23	24.459178075569024	25.33919507729001	25.853863090485817	24.347763756655148
24-25	24.298493722430557	25.551321696497382	25.83933565250848	24.310848928563576
26-27	24.159603522727828	25.767917028784122	25.855345625825414	24.21713382266264
28-29	24.244337028933526	26.00956935549966	25.648783735621077	24.097309879945737
30-31	24.60491114677262	25.674286207425716	25.556539437025037	24.164263208776624
32-33	24.27714571317381	25.65888859526976	25.541937149762656	24.522028541793773
34-35	24.551702347483104	25.727458531312923	25.633408781312	24.08743033989197
36-37	24.582937565954236	25.662261939416283	25.599543511916334	24.15525698271315
38-39	24.51529651283422	25.75544179294294	25.545068997308064	24.184192696914778
40-41	24.699131839085958	25.54405897752921	25.56289445167702	24.193914731707807
42-43	24.480303562956372	25.496461151912765	25.486488782427408	24.53674650270345
44-45	24.43152658449578	25.567491313433095	25.599724407049578	24.401257695021545
46-47	24.49375021194116	25.545062299789052	25.40623034822814	24.55495714004165
48-49	24.329954783857165	25.566603615108935	25.60697966809709	24.496461932936803
50-51	24.4189064327721	25.596521127195615	25.532997855250205	24.45157458478208
52-53	24.58854933370668	25.51876299645907	25.506676195095075	24.38601147473917
54-55	24.165850510661286	25.351859207297355	25.770742813322485	24.71154746871887
56-57	24.23209233946603	25.481709776962912	25.647947139809112	24.638250743761944
58-59	24.359264732029605	25.418735488585035	25.67991367486741	24.54208610451795
60-61	24.401628316373383	25.403108695557286	25.711423094653945	24.483839893415386
62-63	24.33063257515655	25.42132918942834	25.693302337998585	24.554735897416524
64-65	24.130977189982826	25.242029895543443	25.731270613497315	24.895722300976416
66-67	24.17764934303828	25.516667727686627	25.52528466549568	24.780398263779414
68-69	24.226395839848546	25.453500781153686	25.53680592622053	24.783297452777237
70-71	24.143072548218548	25.226629190229428	25.617514567699484	25.012783693852537
72-73	24.153795491600047	25.370694375944275	25.487000528801723	24.988509603653956
74-75	24.10065322469875	25.445538469598404	25.46145685462787	24.992351451074978
76-77	24.005368057901798	25.145373684401807	25.67886481997219	25.170393437724204
78-79	23.988215448616064	25.22698786597803	25.639010326183747	25.14578635922216
80-81	24.123878942504863	25.36960943716694	25.40544027328866	25.10107134703954
82-83	24.067369876088136	25.478551933534852	25.449871896956054	25.004206293420957
84-85	24.073811564082675	25.374316542485133	25.46746528924315	25.08440660418904
86-87	23.897882056187107	25.524967252014854	25.42854589538988	25.148604796408154
88-89	23.895074459756373	25.313017198562154	25.452508228876415	25.339400112805055
90-91	23.829561687141833	25.287528951574178	25.49154536582869	25.3913639954553
92-93	23.83846802169633	25.289049816437114	25.54063627742736	25.331845884439197
94-95	23.72605198672228	25.235526431542045	25.54797795463199	25.490443627103687
96-97	23.595055510084155	25.235652374656343	25.54991710214275	25.619375013116752
98-99	23.49975205565503	25.070228515043357	25.5124155151048	25.917603914196818
100-101	23.473934087657398	25.131101338535476	25.555759049849147	25.839205523957986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	18.0
2	30.5
3	47.5
4	42.0
5	46.5
6	77.5
7	163.0
8	261.5
9	287.0
10	257.0
11	216.5
12	217.0
13	240.0
14	265.5
15	325.0
16	372.5
17	415.0
18	483.5
19	533.0
20	592.5
21	671.5
22	774.0
23	940.0
24	1093.0
25	1334.0
26	1729.5
27	2230.0
28	2829.5
29	3562.0
30	4754.5
31	6830.5
32	10353.0
33	14941.0
34	20770.5
35	28524.0
36	38431.0
37	48877.0
38	59306.5
39	69788.5
40	78634.5
41	86529.5
42	96002.5
43	106038.5
44	114871.0
45	123790.5
46	129819.5
47	134503.5
48	140386.5
49	143154.5
50	141858.0
51	140139.5
52	138493.5
53	136269.5
54	133433.0
55	126357.0
56	115176.0
57	103428.5
58	92896.0
59	81148.0
60	66760.5
61	53812.5
62	43500.0
63	33484.0
64	24351.0
65	17703.0
66	13352.5
67	10470.5
68	7954.5
69	5895.0
70	4533.5
71	3340.5
72	2317.0
73	1666.0
74	1256.0
75	904.5
76	618.0
77	396.0
78	236.5
79	129.5
80	90.5
81	64.5
82	39.0
83	23.0
84	13.5
85	8.5
86	7.0
87	5.5
88	3.5
89	5.5
90	4.0
91	0.5
92	1.5
93	1.5
94	0.0
95	0.0
96	1.0
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16958075179568927
2	1.0072109589924131E-4
3	0.010575715069420337
4	0.006614018630716845
5	0.025616732057040372
6	0.00231658520568255
7	0.0
8	0.0
9	1.0072109589924131E-4
10-11	0.05190493808674235
12-13	3.1895013701426417E-4
14-15	0.005069628493595146
16-17	0.0
18-19	0.0016786849316540219
20-21	4.0288438359696523E-4
22-23	0.0
24-25	6.882608219781488E-4
26-27	0.00307199342492686
28-29	0.00538857863060941
30-31	0.017491896987834904
32-33	0.012187252603808197
34-35	0.010441420274888015
36-37	0.004398154520933536
38-39	0.0
40-41	0.003340583013991503
42-43	0.009837093699492566
44-45	0.007268705754061914
46-47	0.003390943561941124
48-49	0.009115259178881337
50-51	0.0028873380824449174
52-53	0.0022326509590998487
54-55	0.004331007123667375
56-57	0.008410211507586649
58-59	0.01606501479592899
60-61	0.007755524384241581
62-63	0.021973985755351144
64-65	0.04215177863383249
66-67	0.061322360553321416
68-69	0.05086415342911686
70-71	0.06963185096500882
72-73	0.03476556493455479
74-75	0.027966890961356
76-77	0.23065130960926256
78-79	0.08702302685694448
80-81	0.06833926356763523
82-83	0.028235480550420643
84-85	0.05247569096350472
86-87	0.06724811836206011
88-89	0.2953478268752086
90-91	0.29907450742348046
92-93	0.054154375895158736
94-95	0.010072109589924131
96-97	0.015427114521900459
98-99	0.0010407846576254935
100-101	1.8465534248194237E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2978522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.62892082150431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.97967809380823	52.33629203675639
2	11.026619967727399	14.252770976461449
3	3.3200973458795136	6.43722925459601
4	1.529949963285724	3.955160601522259
5	0.8017618786164582	2.5908502485401814
6	0.5225664527260342	2.0263743538323142
7	0.35702597614394777	1.615194248039924
8	0.26180912433781245	1.3536352933740685
9	0.18217844789742565	1.0596596836092542
>10	0.9617445044110619	10.737109113540368
>50	0.043875028466392164	1.9127048453947102
>100	0.01185987473593065	1.3156172977093594
>500	6.770903460344206E-4	0.28110505601064634
>1k	1.5625161802954618E-4	0.1262969906129592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3573698633080436E-5
2	0.0	3.3573698633080436E-5	0.0	3.3573698633080436E-5	3.3573698633080436E-5
3	0.0	3.3573698633080436E-5	0.0	3.3573698633080436E-5	3.3573698633080436E-5
4	0.0	3.3573698633080436E-5	0.0	3.3573698633080436E-5	3.3573698633080436E-5
5	0.0	1.0072109589924131E-4	0.0	3.3573698633080436E-5	3.3573698633080436E-5
6	3.3573698633080436E-5	1.0072109589924131E-4	0.0	3.3573698633080436E-5	3.3573698633080436E-5
7	3.3573698633080436E-5	1.0072109589924131E-4	0.0	3.3573698633080436E-5	3.3573698633080436E-5
8	3.3573698633080436E-5	1.0072109589924131E-4	0.0	3.3573698633080436E-5	3.3573698633080436E-5
9	3.3573698633080436E-5	1.0072109589924131E-4	0.0	1.0072109589924131E-4	3.3573698633080436E-5
10-11	3.3573698633080436E-5	1.0072109589924131E-4	0.0	1.0072109589924131E-4	3.3573698633080436E-5
12-13	3.3573698633080436E-5	1.0072109589924131E-4	0.0	1.3429479453232174E-4	6.714739726616087E-5
14-15	3.3573698633080436E-5	1.0072109589924131E-4	0.0	1.6786849316540218E-4	1.1750794521578152E-4
16-17	3.3573698633080436E-5	1.0072109589924131E-4	0.0	2.1822904111502283E-4	2.3501589043156305E-4
18-19	3.3573698633080436E-5	1.0072109589924131E-4	0.0	2.685895890646435E-4	2.3501589043156305E-4
20-21	3.3573698633080436E-5	1.0072109589924131E-4	0.0	3.189501370142641E-4	2.8537643838118373E-4
22-23	3.3573698633080436E-5	1.0072109589924131E-4	0.0	3.693106849638848E-4	3.525238356473446E-4
24-25	3.3573698633080436E-5	1.0072109589924131E-4	0.0	4.1967123291350547E-4	3.693106849638848E-4
26-27	3.3573698633080436E-5	1.0072109589924131E-4	0.0	6.88260821978149E-4	4.1967123291350547E-4
28-29	3.3573698633080436E-5	1.0072109589924131E-4	0.0	0.0016451112330209414	4.5324493154658585E-4
30-31	3.3573698633080436E-5	1.0072109589924131E-4	0.0	0.007923392877406982	5.036054794962065E-4
32-33	3.3573698633080436E-5	1.0072109589924131E-4	0.0	0.02481096328984644	5.37179178129287E-4
34-35	3.3573698633080436E-5	1.0072109589924131E-4	0.0	0.04874901041523279	6.043265753954478E-4
36-37	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.08579758685683705	6.043265753954478E-4
38-39	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.15187062576673935	6.546871233450685E-4
40-41	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.22650495782807717	6.714739726616087E-4
42-43	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.3085758641366423	6.714739726616087E-4
44-45	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.40630890085753946	6.714739726616087E-4
46-47	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.5219367189498685	6.714739726616087E-4
48-49	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.6453032745771226	7.050476712946891E-4
50-51	6.714739726616087E-5	1.0072109589924131E-4	0.0	0.7784565633559195	7.050476712946891E-4
52-53	6.714739726616087E-5	1.1750794521578152E-4	0.0	0.9159576461077004	8.393424658270108E-4
54-55	6.714739726616087E-5	1.3429479453232174E-4	0.0	1.0710849206418485	9.736372603593326E-4
56-57	6.714739726616087E-5	1.3429479453232174E-4	0.0	1.2517282061371378	9.736372603593326E-4
58-59	6.714739726616087E-5	1.3429479453232174E-4	0.0	1.4488226039626366	9.736372603593326E-4
60-61	6.714739726616087E-5	1.3429479453232174E-4	0.0	1.6615287716525176	9.736372603593326E-4
62-63	6.714739726616087E-5	1.3429479453232174E-4	0.0	1.8763332955069663	0.001007210958992413
64-65	6.714739726616087E-5	1.3429479453232174E-4	0.0	2.109871943198674	0.0010239978083089532
66-67	6.714739726616087E-5	1.3429479453232174E-4	0.0	2.3868549569215873	0.0010407846576254935
68-69	6.714739726616087E-5	1.3429479453232174E-4	0.0	2.684032550372299	0.0010407846576254935
70-71	6.714739726616087E-5	1.3429479453232174E-4	0.0	3.0070786786197985	0.0010407846576254935
72-73	6.714739726616087E-5	1.3429479453232174E-4	0.0	3.3402472770051723	0.0011415057535247347
74-75	6.714739726616087E-5	1.3429479453232174E-4	0.0	3.689229087446727	0.0011415057535247347
76-77	6.714739726616087E-5	1.3429479453232174E-4	0.0	4.078516123097295	0.0011415057535247347
78-79	6.714739726616087E-5	1.3429479453232174E-4	0.0	4.509669560943314	0.0011415057535247347
80-81	6.714739726616087E-5	1.6786849316540218E-4	0.0	4.964106358791374	0.0011415057535247347
82-83	6.714739726616087E-5	1.6786849316540218E-4	0.0	5.441238976915397	0.0012254400001074357
84-85	6.714739726616087E-5	1.6786849316540218E-4	0.0	5.9409331205208495	0.001242226849423976
86-87	6.714739726616087E-5	1.6786849316540218E-4	0.0	6.476702203307546	0.001242226849423976
88-89	6.714739726616087E-5	1.6786849316540218E-4	0.0	7.047975472398727	0.001242226849423976
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1820	0.0	48.57861	1
GTATCAA	3645	0.0	42.643585	1
TCAACGC	4450	0.0	34.238403	4
CAACGCA	4520	0.0	33.628567	5
ATCAACG	4605	0.0	33.293236	3
TATCAAC	4655	0.0	32.73114	2
AACGCAG	4950	0.0	30.797565	6
GTACATG	8575	0.0	27.383965	1
TACATGG	9030	0.0	25.440956	2
ACGCAGA	6360	0.0	23.44631	7
ACATGGG	9405	0.0	23.316656	3
CGCAGAG	6585	0.0	22.428827	8
CATGGGG	8100	0.0	22.032885	4
ATGGGGG	4555	0.0	18.666437	5
GCAGAGT	7740	0.0	18.284561	9
AGAGTAC	6450	0.0	17.670986	10-11
GTATAAA	1005	0.0	17.500046	1
GTATAGC	1040	0.0	17.36816	1
CGTACAC	450	7.2759576E-12	16.876818	3
GAGTACA	6485	0.0	16.052313	1
>>END_MODULE