FastQCFastQC Report
Mon 15 Aug 2016
SRR2921703_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921703_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974337
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.048.720631
GTATCAA44950.045.5072861
TCAACGC54500.036.5788424
CAACGCA56150.035.5015645
TATCAAC57050.035.192862
ATCAACG58000.034.3703423
AACGCAG61000.032.6591156
GTACATG98100.027.5765021
ACGCAGA72350.027.1459587
TACATGG100600.026.5160122
CGCAGAG76800.025.1423458
ACATGGG105000.024.5455683
CATGGGG91550.021.6199594
GCAGAGT85800.020.8465399
GTACATA13650.019.8187161
GAGTACA71000.019.2516271
TAAGGTG18450.018.7790855
GTATAGT6100.018.6730271
AGAGTAC72350.018.45964810-11
TAAGGTA8100.018.1657734
Produced by FastQC (version 0.11.5)