##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921703_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2974337
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.555701321000278	31.0	31.0	34.0	27.0	34.0
2	30.616830574343123	33.0	31.0	34.0	27.0	34.0
3	30.675714957652747	33.0	31.0	34.0	27.0	34.0
4	34.13562350197708	37.0	35.0	37.0	30.0	37.0
5	34.12814553293725	37.0	35.0	37.0	30.0	37.0
6	34.17257694740038	37.0	35.0	37.0	30.0	37.0
7	34.09625405594591	37.0	35.0	37.0	30.0	37.0
8	34.06061922371271	37.0	35.0	37.0	30.0	37.0
9	35.635568867952756	39.0	37.0	39.0	30.0	39.0
10-11	35.53449037550217	39.0	37.0	39.0	30.0	39.0
12-13	35.429779476905274	39.0	37.0	39.0	30.0	39.0
14-15	36.58213023608286	40.0	37.0	41.0	30.0	41.0
16-17	36.524906559007945	40.0	37.0	41.0	29.5	41.0
18-19	36.484962194936216	40.0	37.0	41.0	29.0	41.0
20-21	36.40342688135205	40.0	37.0	41.0	29.0	41.0
22-23	36.304285795456266	40.0	37.0	41.0	28.0	41.0
24-25	36.18949332237739	40.0	36.5	41.0	27.5	41.0
26-27	36.04519998910681	40.0	36.0	41.0	27.0	41.0
28-29	35.905833299992565	40.0	36.0	41.0	27.0	41.0
30-31	35.75823721387321	40.0	36.0	41.0	27.0	41.0
32-33	35.58599008787505	40.0	35.5	41.0	26.0	41.0
34-35	35.41422760769879	39.0	35.0	41.0	25.0	41.0
36-37	35.38393850461464	39.0	35.0	41.0	25.5	41.0
38-39	35.571736491191146	40.0	35.5	41.0	26.0	41.0
40-41	35.634104676100925	40.0	35.5	41.0	26.0	41.0
42-43	35.46805506571717	40.0	35.0	41.0	25.0	41.0
44-45	35.34293356805231	40.0	35.0	41.0	24.5	41.0
46-47	35.17430489551117	40.0	35.0	41.0	24.0	41.0
48-49	35.00360349213959	39.0	35.0	41.0	23.0	41.0
50-51	33.281373798597805	37.0	32.5	39.5	20.5	40.0
52-53	33.39778411121537	37.5	33.5	39.5	21.0	40.0
54-55	34.303925547105116	39.0	34.0	40.5	22.0	41.0
56-57	34.30829206643362	39.0	34.0	41.0	22.0	41.0
58-59	34.1007172354713	38.0	34.0	41.0	21.0	41.0
60-61	33.83154834169766	37.5	34.0	40.0	20.0	41.0
62-63	33.52127885979296	37.0	34.0	40.0	19.5	41.0
64-65	33.214196810919546	36.5	33.5	40.0	18.0	41.0
66-67	32.8624480010167	36.0	33.0	39.0	16.0	41.0
68-69	32.4951365632072	35.5	33.0	39.0	13.0	41.0
70-71	32.08014138949285	35.0	32.5	38.5	9.5	40.0
72-73	31.72158618878762	35.0	32.0	37.0	7.5	39.5
74-75	31.33172165763328	35.0	32.0	37.0	4.5	39.0
76-77	30.966350147948937	35.0	32.0	36.0	2.0	39.0
78-79	30.645871500102373	35.0	32.0	36.0	2.0	37.5
80-81	30.358157128798787	35.0	31.0	35.5	2.0	37.0
82-83	30.07304232842479	35.0	31.0	35.0	2.0	36.5
84-85	29.836782449332407	35.0	31.0	35.0	2.0	36.0
86-87	29.631141494726386	35.0	31.0	35.0	2.0	36.0
88-89	29.437136410568137	35.0	31.0	35.0	2.0	35.5
90-91	29.233394198438173	34.5	31.0	35.0	2.0	35.0
92-93	29.067067047210855	34.0	30.5	35.0	2.0	35.0
94-95	28.856100536018616	34.0	30.0	35.0	2.0	35.0
96-97	28.639341137201335	34.0	30.0	35.0	2.0	35.0
98-99	28.39103874241554	34.0	30.0	35.0	2.0	35.0
100-101	27.32999001121931	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92239.0
3	16339.0
4	11439.0
5	8915.0
6	7984.0
7	8346.0
8	8794.0
9	9415.0
10	10356.0
11	11020.0
12	10904.0
13	11178.0
14	11725.0
15	12426.0
16	12462.0
17	13170.0
18	12593.0
19	11882.0
20	12080.0
21	12718.0
22	13678.0
23	15925.0
24	18540.0
25	21881.0
26	25954.0
27	30865.0
28	36809.0
29	43580.0
30	52276.0
31	64124.0
32	80322.0
33	105134.0
34	146895.0
35	216954.0
36	368624.0
37	668920.0
38	662528.0
39	95335.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49743978568338	17.17321526723309	13.051677241440554	25.277667705642966
2	16.17578729169772	19.1994570993778	38.797140014595335	25.82761559432915
3	19.540973921480823	23.161579292485634	28.420482099173938	28.87696468685961
4	13.888260863714052	15.365779805401914	35.43922510665213	35.3067342242319
5	15.179118421729912	36.41084921069338	33.39263408018856	15.01739828738815
6	34.40342510063738	34.01459426962076	16.898405000060524	14.683575629681336
7	30.145956856865304	30.89684094714087	20.224811287034363	18.732390908959466
8	27.38130625662436	33.746375096170915	19.51778691214686	19.354531735057865
9	27.34801800032816	15.725977819679319	18.636842303461002	38.289161876531516
10-11	26.280086692243042	24.628152584669273	27.12135316833383	21.97040755475386
12-13	27.011579965588478	21.978462282933442	26.812199628511625	24.197758122966455
14-15	24.09082103246568	23.375337007001136	24.134628579269965	28.399213381263216
16-17	24.476873297615523	26.410716486639956	24.914324839717963	24.198085376026558
18-19	24.467888368946422	25.863495096076157	26.0941908450001	23.57442568997733
20-21	25.040075621613493	25.04478347730965	25.938183164506267	23.97695773657059
22-23	24.92441554156712	24.813765174697256	25.72977532426191	24.532043959473715
24-25	24.653202436482736	25.136523715651304	25.62813521090192	24.58213863696404
26-27	24.428820633966517	25.24466265095692	25.708220715513725	24.61829599956284
28-29	24.623027544977028	25.50362887843791	25.43262999663247	24.440713579952593
30-31	24.844199626227052	25.204791664284915	25.316728525035998	24.634280184452038
32-33	24.53123190102816	25.275205242866033	25.32328703975339	24.870275816352418
34-35	24.877672370750307	25.278034150531997	25.35926648813036	24.48502699058734
36-37	25.019277538217604	25.162705448461857	25.32099391574501	24.497023097575525
38-39	24.859518025704176	25.271289159360844	25.270667148584923	24.598525666350056
40-41	24.968530389355738	25.11977616230788	25.37176839711421	24.53992505122217
42-43	24.77801631109627	25.113700195405624	25.27033807296154	24.83794542053656
44-45	24.65017647608709	25.148789848182563	25.41563887206894	24.785394803661408
46-47	24.904521759841717	25.194228503983616	25.194447481114484	24.706802255060182
48-49	24.647870003918264	25.158440389319814	25.238827135142383	24.95486247161954
50-51	24.687180067652584	25.263382541644475	25.367967468007187	24.681469922695754
52-53	25.006565465566894	25.09633420451741	25.30828208844975	24.588818241465948
54-55	24.527666874305368	24.95041769742044	25.556691261015995	24.965224167258196
56-57	24.557985139945544	25.090559142059938	25.508957202045934	24.842498515948584
58-59	24.731200004582274	25.01063441061106	25.415171601903598	24.84299398290307
60-61	24.65867432206588	24.964115316189552	25.62447773464877	24.7527326270958
62-63	24.656015208892338	25.038865316371	25.639603761593264	24.66551571314339
64-65	24.4846475713044	24.83470113804047	25.57462958096663	25.106021709688502
66-67	24.45234074629317	25.109189908492546	25.496932205316945	24.94153713989733
68-69	24.5887242159404	24.947578328479274	25.496849983484793	24.966847472095534
70-71	24.52077305539925	24.802427044576717	25.51622108364448	25.16057881637955
72-73	24.45645024281759	24.90585354539285	25.484974045611743	25.15272216617782
74-75	24.439905154403704	25.0555559579417	25.518599476115693	24.985939411538897
76-77	24.423216915040438	24.66929441277757	25.696857450556276	25.21063122162571
78-79	24.34171447862587	24.786316003330132	25.675350592021502	25.196618926022495
80-81	24.494403981487565	24.857273307758273	25.537168918383884	25.111153792370278
82-83	24.385829805565773	24.908394854106515	25.64096645808655	25.064808882241163
84-85	24.344218852817214	24.944330242871423	25.66723686963624	25.04421403467512
86-87	24.315709888593023	25.02362787707	25.570527506994	25.090134727342978
88-89	24.194419629994933	24.795801698420362	25.688565872338447	25.321212799246258
90-91	24.153525306242322	24.77537006123751	25.761306984392462	25.309797648127702
92-93	24.24526510476525	24.67316514437751	25.904009599949855	25.177560150907386
94-95	24.07484759891306	24.54828973283663	25.973923781848622	25.40293888640169
96-97	24.168337423021356	24.474617478521136	25.944562905535452	25.41248219292206
98-99	23.960005493010314	24.39914302299229	25.918229970100164	25.72262151389723
100-101	24.123366715326156	24.310848695503886	26.113698738397595	25.452085850772367
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	27.0
2	49.0
3	77.0
4	72.0
5	74.5
6	127.5
7	284.5
8	452.0
9	496.0
10	463.0
11	441.5
12	478.0
13	547.5
14	633.0
15	719.5
16	815.0
17	938.0
18	1075.5
19	1164.0
20	1242.5
21	1357.5
22	1448.0
23	1603.0
24	1748.5
25	1919.5
26	2251.5
27	2674.0
28	3201.5
29	3946.0
30	5161.5
31	7248.0
32	10957.5
33	15170.0
34	19805.0
35	26452.5
36	35390.0
37	44897.5
38	53966.0
39	62211.5
40	69678.5
41	77554.0
42	85995.0
43	95626.5
44	105561.0
45	115540.5
46	121852.5
47	127127.5
48	133746.5
49	137217.5
50	136934.5
51	136017.5
52	136470.0
53	136613.0
54	136840.0
55	132818.0
56	123352.5
57	111927.5
58	103080.5
59	91927.5
60	75391.5
61	61715.5
62	51039.5
63	39664.5
64	28964.5
65	21397.5
66	16122.0
67	12452.0
68	9600.5
69	7408.5
70	5786.0
71	4405.0
72	3228.5
73	2408.5
74	1905.0
75	1463.0
76	1064.0
77	723.5
78	474.5
79	338.0
80	258.5
81	190.0
82	153.5
83	121.0
84	100.5
85	90.5
86	74.5
87	55.0
88	46.0
89	34.5
90	27.5
91	24.5
92	20.0
93	22.0
94	21.0
95	14.5
96	11.5
97	11.5
98	10.0
99	5.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05174262365024542
2	0.07241950054751697
3	0.06872119736263914
4	0.06915826955721562
5	0.0644849591690518
6	0.009850934846992792
7	0.012641472704673343
8	0.01563373619061996
9	0.005681938529494136
10-11	0.012389315669340765
12-13	0.020962654870648485
14-15	0.03715113653900012
16-17	0.02523251400228017
18-19	0.00975007203285976
20-21	0.019634627817896894
22-23	0.03387309507967658
24-25	0.03202394348723766
26-27	0.02015575235758423
28-29	0.011649655032365196
30-31	0.011397497997032616
32-33	0.008018593723576044
34-35	0.0051103825827402884
36-37	6.89229229909052E-4
38-39	0.003765545060966528
40-41	0.001311216583729416
42-43	0.22404993112750843
44-45	0.19515273487839474
46-47	0.20140622935464272
48-49	0.207979122742312
50-51	0.19957388823122596
52-53	0.20692006319391515
54-55	0.20307046578783775
56-57	0.2054575523889862
58-59	0.21441753237780387
60-61	0.21843523447410296
62-63	0.2041967672123233
64-65	0.20431444049547848
66-67	0.2017088177970418
68-69	0.19703550740887804
70-71	0.1890673450923685
72-73	0.20026311746113504
74-75	0.19948983588611516
76-77	0.19365660313542143
78-79	0.21134121654674637
80-81	0.22196543296875906
82-83	0.22097361529645093
84-85	0.21477055222726948
86-87	0.2344556114522329
88-89	0.22714305742758809
90-91	0.23388405550547903
92-93	0.2293452288694926
94-95	0.23267370173588264
96-97	0.2393138369996406
98-99	0.23297629017828175
100-101	0.23933064746866275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2974337.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.0182592569629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38239296084657	42.788086915479205
2	12.54218185210256	14.051823892781126
3	4.389153204059953	7.376181663106795
4	2.087405337060687	4.677312533833343
5	1.1646315549231372	3.2620316191262058
6	0.7179711951899135	2.413169793070805
7	0.53082648083243	2.0815182796612617
8	0.3595596701998978	1.611352545888476
9	0.2831470609387361	1.427526492076187
>10	1.4445413929846336	14.326127850970169
>50	0.07149025030362156	2.7051840389908492
>100	0.024774696528031458	2.3011780609936574
>500	0.001503393773423333	0.6069386945612636
>1k	4.2095025645560454E-4	0.37156761946082606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3620938044344E-5
2	0.0	0.0	0.0	0.0	6.7241876088688E-5
3	0.0	0.0	0.0	0.0	6.7241876088688E-5
4	0.0	0.0	0.0	0.0	6.7241876088688E-5
5	0.0	3.3620938044344E-5	0.0	0.0	6.7241876088688E-5
6	0.0	3.3620938044344E-5	0.0	0.0	6.7241876088688E-5
7	0.0	3.3620938044344E-5	0.0	3.3620938044344E-5	6.7241876088688E-5
8	0.0	3.3620938044344E-5	0.0	3.3620938044344E-5	1.34483752177376E-4
9	0.0	3.3620938044344E-5	0.0	3.3620938044344E-5	1.34483752177376E-4
10-11	0.0	3.3620938044344E-5	0.0	5.0431407066516E-5	1.34483752177376E-4
12-13	0.0	3.3620938044344E-5	0.0	1.00862814133032E-4	1.84915159243892E-4
14-15	0.0	5.0431407066516E-5	0.0	1.00862814133032E-4	2.5215703533258E-4
16-17	1.6810469022172E-5	1.00862814133032E-4	0.0	1.1767328315520399E-4	3.02588442399096E-4
18-19	3.3620938044344E-5	1.00862814133032E-4	0.0	1.34483752177376E-4	3.02588442399096E-4
20-21	3.3620938044344E-5	1.00862814133032E-4	0.0	1.51294221199548E-4	3.3620938044344E-4
22-23	3.3620938044344E-5	1.00862814133032E-4	0.0	2.68967504354752E-4	3.3620938044344E-4
24-25	3.3620938044344E-5	1.00862814133032E-4	0.0	3.1939891142126803E-4	3.3620938044344E-4
26-27	3.3620938044344E-5	1.00862814133032E-4	0.0	5.715559467538479E-4	3.3620938044344E-4
28-29	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.0014457003359067918	3.3620938044344E-4
30-31	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.006942723706157037	3.3620938044344E-4
32-33	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.022979911153309125	3.3620938044344E-4
34-35	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.04269859131631688	3.3620938044344E-4
36-37	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.07734496797101337	3.3620938044344E-4
38-39	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.1369548911236353	3.3620938044344E-4
40-41	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.20584419317649616	3.3620938044344E-4
42-43	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.28194518643986877	3.69830318487784E-4
44-45	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.375108805760746	3.86640787509956E-4
46-47	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.4861083327141478	4.202617255543E-4
48-49	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.6001337440915404	4.37072194576472E-4
50-51	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.7255566534659657	4.37072194576472E-4
52-53	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	0.8560227035470426	4.53882663598644E-4
54-55	3.3620938044344E-5	1.00862814133032E-4	3.3620938044344E-5	1.0049802695525087	5.211245396873319E-4
56-57	5.0431407066516E-5	1.00862814133032E-4	3.3620938044344E-5	1.1782121528259912	5.37935008709504E-4
58-59	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	1.3627406712823733	5.71555946753848E-4
60-61	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	1.5610537743369362	6.05176884798192E-4
62-63	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	1.7657716660889469	6.05176884798192E-4
64-65	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	1.9915026441186727	6.387978228425361E-4
66-67	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	2.252367502404738	6.387978228425361E-4
68-69	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	2.544432591195954	6.387978228425361E-4
70-71	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	2.8443313585515027	6.89229229909052E-4
72-73	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	3.1692272933430203	7.73281575019912E-4
74-75	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	3.512278534678485	7.73281575019912E-4
76-77	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	3.894548600242676	7.90092044042084E-4
78-79	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	4.323417285936328	8.06902513064256E-4
80-81	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	4.76218733788404	8.405234511086E-4
82-83	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	5.226240335241098	9.07765327197288E-4
84-85	6.7241876088688E-5	1.00862814133032E-4	3.3620938044344E-5	5.718568541493449	9.07765327197288E-4
86-87	6.7241876088688E-5	1.1767328315520399E-4	3.3620938044344E-5	6.240970676826466	9.41386265241632E-4
88-89	6.7241876088688E-5	1.34483752177376E-4	3.3620938044344E-5	6.797800652716891	9.41386265241632E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2260	0.0	48.72063	1
GTATCAA	4495	0.0	45.507286	1
TCAACGC	5450	0.0	36.578842	4
CAACGCA	5615	0.0	35.501564	5
TATCAAC	5705	0.0	35.19286	2
ATCAACG	5800	0.0	34.370342	3
AACGCAG	6100	0.0	32.659115	6
GTACATG	9810	0.0	27.576502	1
ACGCAGA	7235	0.0	27.145958	7
TACATGG	10060	0.0	26.516012	2
CGCAGAG	7680	0.0	25.142345	8
ACATGGG	10500	0.0	24.545568	3
CATGGGG	9155	0.0	21.619959	4
GCAGAGT	8580	0.0	20.846539	9
GTACATA	1365	0.0	19.818716	1
GAGTACA	7100	0.0	19.251627	1
TAAGGTG	1845	0.0	18.779085	5
GTATAGT	610	0.0	18.673027	1
AGAGTAC	7235	0.0	18.459648	10-11
TAAGGTA	810	0.0	18.165773	4
>>END_MODULE