FastQCFastQC Report
Mon 15 Aug 2016
SRR2921703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974337
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42600.045.758731
GGTATCA22100.045.393111
TCAACGC54650.035.0006684
TATCAAC55750.034.3963662
CAACGCA56000.034.183925
ATCAACG57750.033.2867773
AACGCAG61250.031.4026326
GTACATG91750.027.2570881
TACATGG96350.025.419912
ACGCAGA74200.025.1535027
CGCAGAG76850.023.7917658
ACATGGG102950.022.637263
GCAGAGT87000.020.252189
CATGGGG87500.020.233094
TAAGGTG18400.018.3292795
AGAGTAC74250.018.32505610-11
GTATATG8700.016.9411221
TAAGGTA7650.016.7518884
CAGAGTA81900.016.23645610-11
GTATTAG7100.016.071361
Produced by FastQC (version 0.11.5)