##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2974337
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.361764991660326	33.0	31.0	34.0	28.0	34.0
2	31.49685358451312	34.0	31.0	34.0	28.0	34.0
3	31.568733469005025	34.0	31.0	34.0	28.0	34.0
4	35.051093067127226	37.0	35.0	37.0	32.0	37.0
5	34.79046153815119	37.0	35.0	37.0	32.0	37.0
6	34.76264256538516	37.0	35.0	37.0	32.0	37.0
7	34.650785368302245	37.0	35.0	37.0	32.0	37.0
8	34.59170934564577	37.0	35.0	37.0	32.0	37.0
9	36.17783963283246	39.0	37.0	39.0	32.0	39.0
10-11	36.06625711881337	39.0	37.0	39.0	32.0	39.0
12-13	35.988444147384776	39.0	37.0	39.0	31.0	39.0
14-15	37.225310043885415	40.0	38.0	41.0	31.0	41.0
16-17	37.19399684702843	40.0	38.0	41.0	31.0	41.0
18-19	37.148343479572084	40.0	38.0	41.0	31.0	41.0
20-21	37.08445882225182	40.0	38.0	41.0	31.0	41.0
22-23	36.993539736754784	40.0	37.5	41.0	31.0	41.0
24-25	36.90678510874861	40.0	37.0	41.0	30.0	41.0
26-27	36.77650077983766	40.0	37.0	41.0	30.0	41.0
28-29	36.65173986673332	40.0	37.0	41.0	30.0	41.0
30-31	36.4949834198344	40.0	37.0	41.0	30.0	41.0
32-33	36.45074515766034	40.0	36.5	41.0	30.0	41.0
34-35	36.58479587215571	40.0	37.0	41.0	30.0	41.0
36-37	36.578512959358676	40.0	37.0	41.0	30.0	41.0
38-39	36.473220418533614	40.0	37.0	41.0	30.0	41.0
40-41	36.384001880082856	40.0	37.0	41.0	30.0	41.0
42-43	36.26031297058807	40.0	36.0	41.0	29.0	41.0
44-45	36.12443680726159	40.0	36.0	41.0	28.5	41.0
46-47	35.96156336689488	40.0	36.0	41.0	28.0	41.0
48-49	35.80682686595365	40.0	35.0	41.0	28.0	41.0
50-51	35.63753064968765	39.5	35.0	41.0	27.0	41.0
52-53	35.45531441124526	39.0	35.0	41.0	27.0	41.0
54-55	35.22863885296118	39.0	35.0	41.0	26.0	41.0
56-57	34.98771591786674	39.0	35.0	41.0	26.0	41.0
58-59	34.72860455960438	38.0	35.0	40.0	26.0	41.0
60-61	34.43490599753828	38.0	34.0	40.0	26.0	41.0
62-63	34.11629986783609	37.0	34.0	40.0	25.0	41.0
64-65	33.76651973196043	36.5	34.0	39.5	24.0	41.0
66-67	33.404422061118154	36.0	34.0	39.0	23.0	41.0
68-69	33.034171144695435	35.5	33.0	39.0	22.5	40.5
70-71	32.633157238066836	35.0	33.0	38.0	22.0	40.0
72-73	32.27441981187741	35.0	33.0	37.0	21.5	39.5
74-75	31.881079380043353	35.0	33.0	37.0	20.0	39.0
76-77	29.21601267778332	33.0	28.5	34.5	13.5	36.0
78-79	30.787036909401994	34.5	31.5	35.0	17.5	37.0
80-81	30.983202138829597	35.0	32.0	35.0	18.0	37.0
82-83	30.82608846946395	35.0	32.0	35.0	17.5	36.5
84-85	30.654681866916896	35.0	32.0	35.0	16.0	36.0
86-87	30.464283805096734	35.0	32.0	35.0	11.0	36.0
88-89	30.213941459895096	35.0	32.0	35.0	7.0	35.5
90-91	30.031133997257207	34.0	31.0	35.0	2.0	35.0
92-93	29.884895524616077	34.0	31.0	35.0	2.0	35.0
94-95	29.793656535893547	34.0	31.0	35.0	2.0	35.0
96-97	29.596068972681977	34.0	31.0	35.0	2.0	35.0
98-99	29.400010489732672	34.0	31.0	35.0	2.0	35.0
100-101	28.29034470539149	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56254.0
3	19288.0
4	10456.0
5	7589.0
6	6524.0
7	6544.0
8	6890.0
9	7485.0
10	8173.0
11	8596.0
12	9355.0
13	10027.0
14	10529.0
15	11211.0
16	12156.0
17	12932.0
18	12196.0
19	11248.0
20	10633.0
21	10637.0
22	11299.0
23	12684.0
24	15288.0
25	18141.0
26	22180.0
27	26614.0
28	31962.0
29	39184.0
30	47353.0
31	59354.0
32	75887.0
33	99821.0
34	144305.0
35	218401.0
36	388455.0
37	741660.0
38	704398.0
39	68628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.057121795374776	18.944790986486936	13.751790286368365	26.246296931769926
2	15.240758665741863	20.671634289034124	37.20882068447753	26.87878636074648
3	18.082846762574203	24.492131087458326	28.367526382418557	29.057495767548915
4	13.50797754391911	16.66809564109004	34.54826419955073	35.27566261544012
5	14.873808818400988	36.88537518037761	33.505087901189896	14.735728100031512
6	34.27949139857323	34.74962293319311	16.693922617754787	14.276963050478871
7	29.847492062937054	31.141965419520385	20.428922479194522	18.581620038348042
8	27.297915468220314	34.21364156112774	19.385328562298085	19.10311440835386
9	27.11065595817016	15.728451661143867	18.62842664715755	38.532465733528426
10-11	26.136020966200363	24.958328785420534	27.188619616946053	21.717030631433055
12-13	26.78285079617077	22.216781221858163	27.01589483751049	23.984473144460576
14-15	23.923104247735154	23.61621587726617	24.17691967459578	28.283760200402895
16-17	24.310375724068926	26.76757879150883	24.92856391189028	23.993481572531962
18-19	24.353614970153394	26.076714404085816	26.164600880579457	23.405069745181333
20-21	24.91182463537568	25.227946408744025	25.97920841628623	23.881020539594065
22-23	24.78112345391989	24.991825908063866	25.79608931269209	24.43096132532415
24-25	24.560677115772453	25.27093994378214	25.6903465210469	24.478036419398506
26-27	24.35356707515421	25.419309546948497	25.787837631790982	24.439285746106307
28-29	24.534240065012472	25.60748719360863	25.55570853794728	24.302564203431615
30-31	24.76702907895405	25.382338630249013	25.33610256537864	24.514529725418303
32-33	24.470893110707692	25.438455209294087	25.319374005765262	24.771277674232966
34-35	24.734441793565072	25.430963104208697	25.44400921903031	24.390585883195914
36-37	24.874749136835707	25.348535470176998	25.377265585625324	24.399449807361968
38-39	24.736273115029185	25.41284417549931	25.342458729871353	24.508423979600156
40-41	24.877868041672464	25.251307728153932	25.4237718383373	24.44705239183631
42-43	24.74258192439745	25.260511497273246	25.224231040939753	24.77267553738955
44-45	24.604685654128673	25.229895695214772	25.425146694627877	24.740271956028682
46-47	24.820928803228796	25.31266853067477	25.20512830858076	24.661274357515676
48-49	24.521961252542386	25.37647789450112	25.256844219672715	24.844716633283785
50-51	24.618774473553305	25.42432033013965	25.261220745419905	24.695684450887136
52-53	24.910801762043445	25.217784239111797	25.261240221983584	24.610173776861178
54-55	24.407632415879316	25.132816826255315	25.48375046945255	24.97580028841282
56-57	24.466951909417215	25.21459613493271	25.434479628119668	24.883972327530408
58-59	24.600013450577535	25.13045378885956	25.383896296047208	24.885636464515695
60-61	24.527085413787656	25.134510828449518	25.531150495442333	24.80725326232049
62-63	24.60268686063308	25.175812503877754	25.452539073041656	24.768961562447508
64-65	24.312372650701857	25.037990368373713	25.451158748278747	25.198478232645684
66-67	24.341172946212115	25.292032209789866	25.341820701213713	25.024974142784313
68-69	24.49597391335353	25.10986046442788	25.316782279163984	25.077383343054606
70-71	24.327492718716893	24.969981634042053	25.350943210870064	25.35158243637099
72-73	24.305111212643173	25.126663304561493	25.247554472073535	25.320671010721796
74-75	24.256147881075137	25.17425028783183	25.29298144340159	25.27662038769144
76-77	24.164779357527507	24.874740070271585	25.52537732984817	25.435103242352735
78-79	24.095631276389266	24.955128990272733	25.50263270176308	25.446607031574924
80-81	24.31267570000767	25.050178374617648	25.271212180342058	25.365933745032628
82-83	24.14462984461451	25.054817407587265	25.401999927358528	25.398552820439697
84-85	24.00899080349924	25.096024290777546	25.41464480389045	25.48034010183276
86-87	23.950998661991093	25.141883783311865	25.33461156864638	25.572505986050658
88-89	23.882763601835137	24.955676060621794	25.40564244254073	25.75591789500234
90-91	23.73889596850692	25.00548815586835	25.441488839738092	25.814127035886635
92-93	23.78145120843213	24.877212504360337	25.550950125116934	25.790386162090595
94-95	23.558335202259254	24.78990975021175	25.62413439589197	26.02762065163703
96-97	23.55419073686759	24.712855672945654	25.60929272278526	26.123660867401494
98-99	23.33798762471775	24.65995543176986	25.56781122113454	26.434245722377852
100-101	23.305786513265172	24.603339239425353	25.678724284124492	26.412149963184987
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	28.0
2	37.5
3	55.0
4	52.5
5	55.0
6	94.5
7	243.5
8	407.5
9	434.0
10	367.5
11	329.0
12	340.5
13	339.5
14	358.5
15	400.5
16	448.0
17	517.0
18	609.0
19	677.0
20	720.0
21	814.5
22	968.5
23	1181.5
24	1388.0
25	1629.0
26	2026.5
27	2527.5
28	3158.0
29	4069.0
30	5582.0
31	8086.5
32	12420.0
33	17198.5
34	22226.5
35	28941.5
36	37266.5
37	45795.5
38	54416.0
39	62547.5
40	69963.0
41	78074.0
42	86343.0
43	95873.0
44	105513.5
45	115072.0
46	121866.0
47	127609.0
48	134264.0
49	137724.5
50	137760.5
51	137089.5
52	137677.0
53	138044.0
54	137564.0
55	132968.0
56	123482.5
57	111690.5
58	102284.0
59	91120.5
60	74505.5
61	60422.5
62	49872.0
63	38501.5
64	27799.5
65	20416.0
66	15276.0
67	11783.5
68	9083.5
69	6985.0
70	5400.5
71	3926.0
72	2773.5
73	2096.0
74	1589.5
75	1148.0
76	791.0
77	476.5
78	284.5
79	178.5
80	102.0
81	53.0
82	35.5
83	21.0
84	14.0
85	13.5
86	9.0
87	8.0
88	6.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16272534013462495
2	3.3620938044344E-4
3	0.009817313908948447
4	0.0059172850958045435
5	0.024005349763661615
6	0.002118119096793672
7	0.0
8	0.0
9	3.3620938044344004E-5
10-11	0.04577490714737436
12-13	2.1853609728823598E-4
14-15	0.004320290538698204
16-17	0.0
18-19	0.0015297526810176519
20-21	2.8577797337692396E-4
22-23	1.6810469022172002E-5
24-25	5.21124539687332E-4
26-27	0.00243751800821494
28-29	0.004656499919141644
30-31	0.01573459900475299
32-33	0.010624216422012705
34-35	0.009128084679039396
36-37	0.003563819432700464
38-39	1.6810469022172002E-5
40-41	0.00319398911421268
42-43	0.009548346404593696
44-45	0.0072789330866004755
46-47	0.003395714742478744
48-49	0.008674202015440752
50-51	0.002706485512569692
52-53	0.002118119096793672
54-55	0.0043034800696760325
56-57	0.008808685767618127
58-59	0.01637339682759553
60-61	0.007631952936066088
62-63	0.021635073631535363
64-65	0.04084943972387796
66-67	0.0592569033031563
68-69	0.0495908836154074
70-71	0.06690566670824456
72-73	0.03367136945141052
74-75	0.0273170121610295
76-77	0.22552925240145955
78-79	0.08354803104019484
80-81	0.06525824074407172
82-83	0.027871757638761173
84-85	0.05115425723446939
86-87	0.06757808546913144
88-89	0.2915271537825068
90-91	0.29788151107288785
92-93	0.05103658395131419
94-95	0.008674202015440752
96-97	0.013347512403604569
98-99	7.06039698931224E-4
100-101	2.35346566310408E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2974337.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.462953284648854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75700677581015	42.01707327791585
2	12.88848504239731	14.296668876327551
3	4.5560286972671795	7.580724204001475
4	2.12198721203032	4.7076671044583955
5	1.1810939853445386	3.275348026697194
6	0.7224584833229287	2.4041808666382716
7	0.5161564116535582	2.0039291262979297
8	0.38623859320077575	1.7137546441138494
9	0.272910535948614	1.3622781875585812
>10	1.495945965319332	14.612916907234224
>50	0.07542504104715235	2.8276009730725202
>100	0.024316894792026898	2.2464048799378973
>500	0.0015205952080754466	0.5866798267273186
>1k	4.2576665813898103E-4	0.36477309901881305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3620938044344E-5
2	0.0	0.0	0.0	0.0	3.3620938044344E-5
3	0.0	0.0	0.0	0.0	3.3620938044344E-5
4	0.0	0.0	0.0	3.3620938044344E-5	3.3620938044344E-5
5	0.0	0.0	0.0	3.3620938044344E-5	3.3620938044344E-5
6	0.0	0.0	0.0	3.3620938044344E-5	3.3620938044344E-5
7	0.0	0.0	0.0	3.3620938044344E-5	3.3620938044344E-5
8	0.0	0.0	0.0	3.3620938044344E-5	6.7241876088688E-5
9	0.0	0.0	0.0	6.7241876088688E-5	6.7241876088688E-5
10-11	0.0	0.0	0.0	8.405234511085999E-5	6.7241876088688E-5
12-13	0.0	0.0	0.0	1.00862814133032E-4	6.7241876088688E-5
14-15	0.0	0.0	0.0	1.00862814133032E-4	6.7241876088688E-5
16-17	0.0	0.0	0.0	1.1767328315520399E-4	6.7241876088688E-5
18-19	0.0	0.0	0.0	1.34483752177376E-4	6.7241876088688E-5
20-21	0.0	0.0	0.0	1.34483752177376E-4	8.405234511085999E-5
22-23	0.0	0.0	0.0	2.01725628266064E-4	1.00862814133032E-4
24-25	0.0	0.0	0.0	3.02588442399096E-4	1.00862814133032E-4
26-27	0.0	0.0	0.0	5.379350087095041E-4	1.00862814133032E-4
28-29	0.0	0.0	0.0	0.001445700335906792	1.00862814133032E-4
30-31	0.0	0.0	0.0	0.007043586520290068	1.1767328315520399E-4
32-33	0.0	0.0	0.0	0.023416983347885598	1.34483752177376E-4
34-35	0.0	0.0	0.0	0.0432869577320929	1.51294221199548E-4
36-37	0.0	0.0	0.0	0.07798376579385591	1.6810469022172E-4
38-39	0.0	0.0	0.0	0.13774498316767736	1.6810469022172E-4
40-41	0.0	0.0	0.0	0.2076597238308907	1.6810469022172E-4
42-43	0.0	0.0	0.0	0.28330683443066473	1.6810469022172E-4
44-45	0.0	0.0	0.0	0.37605019202598766	1.84915159243892E-4
46-47	0.0	0.0	0.0	0.4870497189793893	2.18536097288236E-4
48-49	0.0	0.0	0.0	0.6010246989497154	2.35346566310408E-4
50-51	0.0	0.0	0.0	0.7261282094127195	2.35346566310408E-4
52-53	0.0	0.0	0.0	0.8575692666970824	2.6896750435475203E-4
54-55	0.0	0.0	0.0	1.005988897693839	3.02588442399096E-4
56-57	0.0	0.0	0.0	1.1792039704982993	3.02588442399096E-4
58-59	0.0	0.0	0.0	1.3635980052025038	3.1939891142126803E-4
60-61	0.0	3.3620938044344E-5	0.0	1.5615580884076015	3.69830318487784E-4
62-63	0.0	3.3620938044344E-5	0.0	1.7666121895400555	4.03451256532128E-4
64-65	0.0	3.3620938044344E-5	0.0	1.9925448931980472	4.03451256532128E-4
66-67	0.0	3.3620938044344E-5	0.0	2.2524851756878927	4.202617255543E-4
68-69	0.0	3.3620938044344E-5	0.0	2.5429028385149364	4.53882663598644E-4
70-71	0.0	3.3620938044344E-5	0.0	2.8416584939769773	4.70693132620816E-4
72-73	0.0	3.3620938044344E-5	0.0	3.16682339627285	5.0431407066516E-4
74-75	0.0	3.3620938044344E-5	0.0	3.510731971528445	5.0431407066516E-4
76-77	0.0	3.3620938044344E-5	0.0	3.8943132536763656	5.0431407066516E-4
78-79	0.0	3.3620938044344E-5	0.0	4.324543587360814	5.0431407066516E-4
80-81	0.0	3.3620938044344E-5	0.0	4.765196411839009	5.54745477731676E-4
82-83	0.0	3.3620938044344E-5	0.0	5.22997225936402	6.387978228425361E-4
84-85	0.0	3.3620938044344E-5	0.0	5.724115996270765	6.387978228425361E-4
86-87	0.0	3.3620938044344E-5	0.0	6.247560380683157	6.55608291864708E-4
88-89	0.0	3.3620938044344E-5	0.0	6.807147273493219	7.06039698931224E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4260	0.0	45.75873	1
GGTATCA	2210	0.0	45.39311	1
TCAACGC	5465	0.0	35.000668	4
TATCAAC	5575	0.0	34.396366	2
CAACGCA	5600	0.0	34.18392	5
ATCAACG	5775	0.0	33.286777	3
AACGCAG	6125	0.0	31.402632	6
GTACATG	9175	0.0	27.257088	1
TACATGG	9635	0.0	25.41991	2
ACGCAGA	7420	0.0	25.153502	7
CGCAGAG	7685	0.0	23.791765	8
ACATGGG	10295	0.0	22.63726	3
GCAGAGT	8700	0.0	20.25218	9
CATGGGG	8750	0.0	20.23309	4
TAAGGTG	1840	0.0	18.329279	5
AGAGTAC	7425	0.0	18.325056	10-11
GTATATG	870	0.0	16.941122	1
TAAGGTA	765	0.0	16.751888	4
CAGAGTA	8190	0.0	16.236456	10-11
GTATTAG	710	0.0	16.07136	1
>>END_MODULE