FastQCFastQC Report
Mon 15 Aug 2016
SRR2921702_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921702_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805427
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA36690.1307822302986319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.051.096211
GTATCAA44750.047.19661
TCAACGC55750.037.2078484
TATCAAC57400.036.136352
CAACGCA58000.035.6775055
ATCAACG58750.035.2251933
AACGCAG64850.032.2547386
ACGCAGA74700.027.1184657
GTACATG95100.026.3510041
CGCAGAG77350.025.2098548
TACATGG100000.024.7768022
TATACCG2503.8744474E-1022.7812335
ACATGGG106200.022.6599123
TAAGGTA7250.022.2607044
CATGGGG91250.020.5476534
GTAAGTA10900.020.466622
GCAGAGT89350.020.3380159
GAGTACA76600.019.0218751
AGAGTAC73650.018.00583810-11
GTGGTAT13450.017.9966491
Produced by FastQC (version 0.11.5)