##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921702_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2805427
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.610799354251597	31.0	31.0	34.0	27.0	34.0
2	30.666307838343325	33.0	31.0	34.0	27.0	34.0
3	30.72369375499701	33.0	31.0	34.0	27.0	34.0
4	34.191070735399634	37.0	35.0	37.0	30.0	37.0
5	34.18806548878299	37.0	35.0	37.0	30.0	37.0
6	34.23767754427401	37.0	35.0	37.0	30.0	37.0
7	34.16086285617127	37.0	35.0	37.0	30.0	37.0
8	34.12863175552242	37.0	35.0	37.0	30.0	37.0
9	35.70162474375559	39.0	37.0	39.0	30.0	39.0
10-11	35.60362825338175	39.0	37.0	39.0	30.0	39.0
12-13	35.49661121105628	39.0	37.0	39.0	30.0	39.0
14-15	36.649531069601885	40.0	37.0	41.0	30.0	41.0
16-17	36.59036627935782	40.0	37.0	41.0	30.0	41.0
18-19	36.55671881677905	40.0	37.0	41.0	29.5	41.0
20-21	36.477323238138084	40.0	37.0	41.0	29.0	41.0
22-23	36.38244641546545	40.0	37.0	41.0	29.0	41.0
24-25	36.27031464372446	40.0	36.0	41.0	28.0	41.0
26-27	36.117692066127546	40.0	36.0	41.0	27.5	41.0
28-29	35.97597478030974	40.0	36.0	41.0	27.0	41.0
30-31	35.8241123365534	40.0	36.0	41.0	27.0	41.0
32-33	35.647259935831514	39.5	35.5	41.0	26.5	41.0
34-35	35.480750523895296	39.0	35.0	41.0	25.5	41.0
36-37	35.45103633065483	39.0	35.0	41.0	25.5	41.0
38-39	35.63302395678091	40.0	35.0	41.0	26.5	41.0
40-41	35.693231547283176	40.0	35.0	41.0	26.5	41.0
42-43	35.53278217540503	40.0	35.0	41.0	26.0	41.0
44-45	35.40033674018251	40.0	35.0	41.0	25.0	41.0
46-47	35.23215681605688	40.0	35.0	41.0	24.0	41.0
48-49	35.05602908220389	39.0	35.0	41.0	24.0	41.0
50-51	33.33753382283695	37.0	32.5	39.5	20.5	40.0
52-53	33.45723110956015	37.5	33.5	39.5	21.5	40.0
54-55	34.36775489078846	39.0	34.0	40.5	22.0	41.0
56-57	34.37984039506286	39.0	34.0	41.0	22.0	41.0
58-59	34.17667880860917	38.0	34.0	41.0	22.0	41.0
60-61	33.91078648633523	38.0	34.0	40.0	21.0	41.0
62-63	33.597802936950416	37.0	34.0	40.0	20.0	41.0
64-65	33.29643027603285	36.5	33.5	40.0	19.5	41.0
66-67	32.9425757647588	36.0	33.0	39.0	18.0	41.0
68-69	32.57178550716165	35.5	33.0	39.0	16.0	41.0
70-71	32.153638465730886	35.0	33.0	38.5	12.5	40.0
72-73	31.786033819450658	35.0	32.0	37.0	9.5	39.5
74-75	31.390947438660852	35.0	32.0	37.0	7.0	39.0
76-77	31.016193256855374	35.0	32.0	36.0	3.5	39.0
78-79	30.692327228617962	35.0	32.0	36.0	2.0	37.0
80-81	30.398737340162477	35.0	31.0	35.0	2.0	37.0
82-83	30.109776693530076	35.0	31.0	35.0	2.0	36.5
84-85	29.87622240749804	35.0	31.0	35.0	2.0	36.0
86-87	29.671387991917094	35.0	31.0	35.0	2.0	36.0
88-89	29.477391676917634	35.0	31.0	35.0	2.0	35.5
90-91	29.274947271841327	34.5	31.0	35.0	2.0	35.0
92-93	29.104236538680208	34.0	31.0	35.0	2.0	35.0
94-95	28.893300199933915	34.0	30.0	35.0	2.0	35.0
96-97	28.67732737298101	34.0	30.0	35.0	2.0	35.0
98-99	28.429922788937297	34.0	30.0	35.0	2.0	35.0
100-101	27.37226062200157	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80544.0
3	14650.0
4	10422.0
5	8188.0
6	7428.0
7	7806.0
8	8378.0
9	8894.0
10	9683.0
11	10396.0
12	10474.0
13	10578.0
14	11037.0
15	11931.0
16	11924.0
17	12622.0
18	12369.0
19	11521.0
20	11614.0
21	12206.0
22	13293.0
23	15031.0
24	17938.0
25	20914.0
26	25108.0
27	29880.0
28	34978.0
29	41903.0
30	49858.0
31	61479.0
32	76523.0
33	100010.0
34	140779.0
35	206487.0
36	346299.0
37	626110.0
38	633635.0
39	82532.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71619380494677	17.14959787435134	13.147279633361272	24.98692868734062
2	16.37527160545355	19.549670934230008	38.3865949353587	25.688462524957735
3	19.754892902980938	23.394293818886432	28.373058480829695	28.47775479730294
4	14.135078668126344	15.701913311851962	35.402565279723355	34.76044274029834
5	15.241931255290256	36.64208670367037	33.05453926748072	15.06144277355865
6	34.10690330904096	33.86677209244624	17.298680668617138	14.72764392989566
7	30.007461518634727	31.12701016270959	20.13626094852194	18.729267370133748
8	27.538385957306634	32.95324093418308	19.881801056139686	19.626572052370612
9	27.530269986247234	15.80277579142566	18.567801703517755	38.09915251880935
10-11	26.23455409247144	24.666251822814502	27.136527535929122	21.96266654878494
12-13	27.112883383743753	22.05018841736367	26.766157642379046	24.070770556513533
14-15	24.269277201555667	23.430467068866196	24.28817654986691	28.01207917971123
16-17	24.425227316971597	26.406794515155774	25.04364143568914	24.12433673218349
18-19	24.377742945032193	25.914130825755905	26.2378745386612	23.470251690550707
20-21	25.04186475997954	25.108017676430062	26.054248600200008	23.795868963390397
22-23	24.880517219676797	24.76369991359941	25.92254827182774	24.433234594896053
24-25	24.69735857775825	24.997397016338894	25.783783340476678	24.521461065426173
26-27	24.34609105685376	25.233763645497127	25.827518135466864	24.592627162182254
28-29	24.534814578210256	25.61564255050946	25.42135482357605	24.428188047704232
30-31	24.985513053580934	25.184043486328438	25.313913546752065	24.516529913338562
32-33	24.605594004989666	25.240040859839024	25.266384797231	24.887980337940313
34-35	24.929868991943955	25.297585575514507	25.327368640375465	24.445176792166073
36-37	25.02864553025465	25.222948576349673	25.370894786736276	24.377511106659398
38-39	24.841837127531246	25.34953032364038	25.309855931695886	24.498776617132492
40-41	25.021681423342223	25.1190294992117	25.392021164918905	24.467267912527173
42-43	24.831666026253544	25.113197708660795	25.233241674763384	24.821894590322277
44-45	24.68495091597149	25.125636576213335	25.45067283273	24.738739675085178
46-47	24.878338535426174	25.090202450320565	25.22900430032134	24.802454713931922
48-49	24.59064060621572	25.186611515662545	25.337318856045915	24.885429022075822
50-51	24.663413010291734	25.287921443629045	25.43918646208445	24.609479083994767
52-53	25.002964798200832	24.981353919748987	25.344077333367625	24.67160394868256
54-55	24.31870961358525	24.864449167745843	25.745040667482144	25.07180055118677
56-57	24.46693844017485	24.975473408316624	25.58895521988203	24.968632931626487
58-59	24.693510036166494	24.924855569797334	25.47249717426935	24.909137219766826
60-61	24.655470011408866	24.904600145866258	25.731139873521848	24.708789969203035
62-63	24.628889701711547	24.94324132403096	25.677335678453705	24.750533295803788
64-65	24.35783406541554	24.756219662524217	25.6809936171862	25.204952654874052
66-67	24.414125042974312	24.952694768519137	25.578257899460198	25.05492228904635
68-69	24.558979134986377	24.869003217214196	25.518571531895777	25.053446115903643
70-71	24.37017226413346	24.68709727015571	25.64746146605851	25.295268999652325
72-73	24.30310959760859	24.876602277326	25.617327937015816	25.202960188049595
74-75	24.376365317053587	24.934796957977166	25.64101144544661	25.04782627952264
76-77	24.229934656793247	24.465353064216206	25.84848754878954	25.456224730201004
78-79	24.20552277855017	24.70818621784476	25.835611212164945	25.250679791440128
80-81	24.40036790784655	24.784674055190823	25.59449645873514	25.22046157822749
82-83	24.292212059595787	24.814935422773935	25.758867031987716	25.13398548564257
84-85	24.175460262745986	24.830655139504657	25.81556409794289	25.178320499806468
86-87	24.03142544496304	24.932186962638735	25.75376850755935	25.282619084838874
88-89	23.971018707646444	24.649265124375248	25.883692538771356	25.496023629206952
90-91	23.95487135950646	24.675899847447603	25.94807501526149	25.42115377778445
92-93	24.11004964871462	24.453153136415345	25.994867917840953	25.44192929702908
94-95	23.86243311569602	24.448297725125272	26.058719733797297	25.630549425381403
96-97	23.896609537204185	24.311734337279653	26.147180903294416	25.644475222221747
98-99	23.627486955797814	24.157205012375154	26.17949851237319	26.035809519453846
100-101	23.84777817024145	24.148932996088522	26.38086616315781	25.62242267051221
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	51.0
2	70.5
3	87.5
4	85.0
5	103.5
6	170.0
7	373.0
8	578.0
9	605.0
10	515.0
11	444.5
12	470.0
13	548.5
14	645.0
15	753.5
16	852.0
17	933.5
18	1047.5
19	1196.5
20	1324.5
21	1416.0
22	1488.0
23	1531.0
24	1656.0
25	1873.5
26	2073.5
27	2392.0
28	2740.5
29	3112.5
30	3740.5
31	4766.5
32	6685.5
33	9035.0
34	12180.0
35	17775.5
36	26205.0
37	35786.0
38	46217.5
39	56897.5
40	66099.5
41	74992.0
42	85157.5
43	95953.5
44	105869.5
45	115302.5
46	122713.0
47	128795.0
48	134642.0
49	138635.5
50	138620.5
51	137144.5
52	135356.0
53	132735.0
54	130944.0
55	125225.5
56	114214.0
57	101665.0
58	93221.0
59	82397.5
60	66313.5
61	54365.0
62	45104.5
63	34342.0
64	24675.0
65	18177.5
66	13539.0
67	10359.5
68	7911.5
69	6029.0
70	4517.0
71	3190.0
72	2249.0
73	1649.5
74	1236.0
75	953.5
76	721.0
77	464.0
78	322.5
79	229.5
80	156.5
81	131.5
82	123.0
83	96.0
84	64.0
85	53.0
86	55.5
87	54.5
88	44.5
89	30.0
90	23.5
91	21.0
92	19.5
93	15.5
94	17.0
95	15.0
96	6.0
97	3.0
98	3.0
99	4.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05621247674596416
2	0.07799169253022802
3	0.07417765637815563
4	0.07371426880827767
5	0.06858136034193725
6	0.010479688118778354
7	0.013117432747314401
8	0.01764437285304519
9	0.006166619199145086
10-11	0.012671867776277906
12-13	0.021975264371519917
14-15	0.03912060445700423
16-17	0.026840833855238434
18-19	0.0098380745604858
20-21	0.0200326010978008
22-23	0.037356167171699706
24-25	0.03396987339182235
26-27	0.020103891493166638
28-29	0.011014366084022148
30-31	0.011246059868961125
32-33	0.008109282472864202
34-35	0.005043795472133119
36-37	5.525005640852534E-4
38-39	0.003243712989145681
40-41	0.001069355930487587
42-43	0.2300006380490385
44-45	0.19909625165794725
46-47	0.2050489996709948
48-49	0.21249884598672503
50-51	0.20335585278105614
52-53	0.2106987635037376
54-55	0.20786497028794548
56-57	0.2108235216956278
58-59	0.21925361094763826
60-61	0.2241191804313568
62-63	0.2087026324334941
64-65	0.20952247198020124
66-67	0.20636787198526288
68-69	0.2011993183212395
70-71	0.19232366409819254
72-73	0.20460343469995831
74-75	0.20356972396715364
76-77	0.19670802341319166
78-79	0.21526134880715128
80-81	0.22657869907147823
82-83	0.22586579511781985
84-85	0.2204120798723332
86-87	0.24023080978403644
88-89	0.23233539849726975
90-91	0.2394109702373293
92-93	0.23393943239300116
94-95	0.23730790357403705
96-97	0.24491815327934036
98-99	0.23789604933580522
100-101	0.2447577498897672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2805427.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.63107604511447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51467959723614	46.0340015012335
2	11.725583176973696	13.749671178449194
3	3.855882571437535	6.782236328009569
4	1.7986896153249652	4.21836430470703
5	0.996292941289799	2.920686360198648
6	0.6558263780224908	2.307108374533719
7	0.4437802475990557	1.8213519411009977
8	0.32192537629163304	1.5099864974565462
9	0.25848893491323516	1.3639935959746683
>10	1.345667536461702	13.918595415560088
>50	0.06308891757618458	2.477421771339409
>100	0.018265797216413398	1.8157663315758354
>500	0.001219273104832115	0.4960503339495119
>1k	6.096365523478836E-4	0.5847660659112551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA	3669	0.1307822302986319	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	3.564519768291957E-5	1.0693559304875871E-4
7	0.0	0.0	0.0	3.564519768291957E-5	1.0693559304875871E-4
8	0.0	0.0	0.0	3.564519768291957E-5	1.0693559304875871E-4
9	0.0	0.0	0.0	3.564519768291957E-5	1.4258079073167828E-4
10-11	0.0	0.0	0.0	3.564519768291957E-5	1.4258079073167828E-4
12-13	0.0	0.0	0.0	3.564519768291957E-5	1.4258079073167828E-4
14-15	0.0	0.0	0.0	3.564519768291957E-5	1.7822598841459784E-4
16-17	0.0	0.0	0.0	3.564519768291957E-5	2.49516383780437E-4
18-19	0.0	0.0	0.0	3.564519768291957E-5	2.8516158146335655E-4
20-21	0.0	0.0	0.0	3.564519768291957E-5	3.0298418030481634E-4
22-23	0.0	0.0	0.0	1.247581918902185E-4	3.564519768291957E-4
24-25	0.0	0.0	0.0	4.812101687194142E-4	4.633875698779544E-4
26-27	0.0	0.0	0.0	7.841943490242305E-4	4.633875698779544E-4
28-29	0.0	0.0	0.0	0.0019604858725605764	4.812101687194142E-4
30-31	0.0	0.0	0.0	0.008519202246217777	5.168553664023337E-4
32-33	0.0	0.0	0.0	0.02719728583206763	5.703231629267131E-4
34-35	0.0	0.0	0.0	0.053610377315111035	5.703231629267131E-4
36-37	0.0	0.0	0.0	0.0930161433535786	5.703231629267131E-4
38-39	0.0	0.0	0.0	0.16188266527697923	5.881457617681728E-4
40-41	0.0	0.0	0.0	0.24539936344805977	6.059683606096327E-4
42-43	0.0	0.0	0.0	0.3398413147089552	6.772587559754718E-4
44-45	0.0	1.7822598841459784E-5	0.0	0.4562050625448461	7.48549151341311E-4
46-47	0.0	3.564519768291957E-5	0.0	0.5944193165603668	7.663717501827708E-4
48-49	0.0	3.564519768291957E-5	0.0	0.7365188971233256	8.554847443900697E-4
50-51	0.0	3.564519768291957E-5	0.0	0.8924131691895744	8.554847443900697E-4
52-53	0.0	3.564519768291957E-5	0.0	1.0544384152572852	9.624203374388284E-4
54-55	0.0	3.564519768291957E-5	0.0	1.2379577155277968	0.0011050011281705067
56-57	0.0	3.564519768291957E-5	0.0	1.4579776982256178	0.0011406463258534262
58-59	0.0	3.564519768291957E-5	0.0	1.6790848594527676	0.0011762915235363457
60-61	0.0	3.564519768291957E-5	0.0	1.9189413946611336	0.0012119367212192653
62-63	0.0	3.564519768291957E-5	0.0	2.168475601040412	0.0012832271165851045
64-65	0.0	3.564519768291957E-5	0.0	2.4422485418440756	0.0012832271165851045
66-67	0.0	3.564519768291957E-5	0.0	2.770309118718826	0.001318872314268024
68-69	0.0	3.564519768291957E-5	0.0	3.1243193995067418	0.0013545175119509436
70-71	0.0	5.3467796524379353E-5	0.0	3.5035308350564813	0.0013545175119509436
72-73	0.0	7.129039536583914E-5	0.0	3.9082642321471917	0.0015327435003655416
74-75	0.0	7.129039536583914E-5	0.0	4.332174745591313	0.0015327435003655416
76-77	0.0	7.129039536583914E-5	0.0	4.811281847647434	0.0015327435003655416
78-79	0.0	7.129039536583914E-5	0.0	5.336923755278608	0.0015327435003655416
80-81	0.0	7.129039536583914E-5	0.0	5.879835761187156	0.001568388698048461
82-83	0.0	7.129039536583914E-5	0.0	6.452190700381796	0.0016040338957313807
84-85	0.0	7.129039536583914E-5	0.0	7.0639157604172205	0.0016040338957313807
86-87	0.0	7.129039536583914E-5	0.0	7.71454755372355	0.0016040338957313807
88-89	0.0	7.129039536583914E-5	0.0	8.415938108530359	0.0016040338957313807
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2220	0.0	51.09621	1
GTATCAA	4475	0.0	47.1966	1
TCAACGC	5575	0.0	37.207848	4
TATCAAC	5740	0.0	36.13635	2
CAACGCA	5800	0.0	35.677505	5
ATCAACG	5875	0.0	35.225193	3
AACGCAG	6485	0.0	32.254738	6
ACGCAGA	7470	0.0	27.118465	7
GTACATG	9510	0.0	26.351004	1
CGCAGAG	7735	0.0	25.209854	8
TACATGG	10000	0.0	24.776802	2
TATACCG	250	3.8744474E-10	22.781233	5
ACATGGG	10620	0.0	22.659912	3
TAAGGTA	725	0.0	22.260704	4
CATGGGG	9125	0.0	20.547653	4
GTAAGTA	1090	0.0	20.46662	2
GCAGAGT	8935	0.0	20.338015	9
GAGTACA	7660	0.0	19.021875	1
AGAGTAC	7365	0.0	18.005838	10-11
GTGGTAT	1345	0.0	17.996649	1
>>END_MODULE