FastQCFastQC Report
Mon 15 Aug 2016
SRR2921702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805427
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA35060.12497206307631602No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44500.047.435381
GGTATCA20750.046.9694141
TATCAAC57700.036.5262032
TCAACGC57350.036.4167444
ATCAACG59800.035.164133
CAACGCA59750.035.1416855
AACGCAG63850.032.951896
GTACATG89650.027.1518251
ACGCAGA78050.026.5912197
TACATGG96050.025.5005572
CGCAGAG81550.025.1587778
ACATGGG100100.023.4255733
CATGGGG84550.021.3891374
GCAGAGT91600.020.99939
TATACCG1908.250878E-620.0020545
GTGGTAT11550.019.3461821
ATGGGGG49700.019.2122365
TAAGGTA6900.018.573624
AGAGTAC76750.018.47188610-11
TAAGGCG5450.017.4329835
Produced by FastQC (version 0.11.5)