##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921702_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2805427
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422538886237284	33.0	31.0	34.0	29.0	34.0
2	31.5580687004153	34.0	31.0	34.0	29.0	34.0
3	31.62744922608929	34.0	31.0	34.0	28.0	34.0
4	35.112662350508494	37.0	35.0	37.0	32.0	37.0
5	34.86166740392817	37.0	35.0	37.0	32.0	37.0
6	34.8312043050844	37.0	35.0	37.0	32.0	37.0
7	34.72610515262026	37.0	35.0	37.0	32.0	37.0
8	34.66174525303991	37.0	35.0	37.0	32.0	37.0
9	36.25049520090881	39.0	37.0	39.0	32.0	39.0
10-11	36.14906803848398	39.0	37.0	39.0	32.0	39.0
12-13	36.07207993649452	39.0	37.0	39.0	31.5	39.0
14-15	37.30452850849443	40.0	38.0	41.0	32.0	41.0
16-17	37.27253819115593	40.0	38.0	41.0	31.0	41.0
18-19	37.230458144161304	40.0	38.0	41.0	31.0	41.0
20-21	37.168506434136404	40.0	38.0	41.0	31.0	41.0
22-23	37.08162393817412	40.0	37.5	41.0	31.0	41.0
24-25	36.993084475197534	40.0	37.0	41.0	30.5	41.0
26-27	36.86230955216443	40.0	37.0	41.0	30.0	41.0
28-29	36.735428332300216	40.0	37.0	41.0	30.0	41.0
30-31	36.573133073860056	40.0	37.0	41.0	30.0	41.0
32-33	36.5290258844732	40.0	36.5	41.0	30.0	41.0
34-35	36.6631514560885	40.0	37.0	41.0	30.0	41.0
36-37	36.658231884130295	40.0	37.0	41.0	30.0	41.0
38-39	36.552475790672865	40.0	37.0	41.0	30.0	41.0
40-41	36.46570236901548	40.0	37.0	41.0	30.0	41.0
42-43	36.34004698749958	40.0	36.0	41.0	29.5	41.0
44-45	36.20341627139113	40.0	36.0	41.0	29.0	41.0
46-47	36.03733959215478	40.0	36.0	41.0	28.0	41.0
48-49	35.885048336670316	40.0	35.0	41.0	28.0	41.0
50-51	35.71884208714039	39.5	35.0	41.0	28.0	41.0
52-53	35.534542691718585	39.0	35.0	41.0	27.0	41.0
54-55	35.3081363371779	39.0	35.0	41.0	27.0	41.0
56-57	35.073042713283925	39.0	35.0	41.0	26.0	41.0
58-59	34.816522939288745	38.0	35.0	40.0	26.0	41.0
60-61	34.524890863315996	38.0	34.0	40.0	26.0	41.0
62-63	34.20539155002073	37.0	34.0	40.0	26.0	41.0
64-65	33.857411902002795	37.0	34.0	39.5	25.0	41.0
66-67	33.49517399668571	36.0	34.0	39.0	24.0	41.0
68-69	33.120374189027196	35.5	33.5	39.0	23.0	40.5
70-71	32.71374179402993	35.0	33.0	38.0	22.0	40.0
72-73	32.34648130213333	35.0	33.0	37.0	22.0	39.0
74-75	31.947608510219652	35.0	33.0	37.0	20.5	39.0
76-77	29.263521381950056	33.0	29.0	34.5	15.0	36.0
78-79	30.840639588911063	34.5	31.5	35.0	18.0	37.0
80-81	31.030190591307488	35.0	32.0	35.0	19.0	37.0
82-83	30.86725264995311	35.0	32.0	35.0	18.0	36.5
84-85	30.690220419208913	35.0	32.0	35.0	17.5	36.0
86-87	30.50215296994005	35.0	32.0	35.0	13.5	36.0
88-89	30.255120699986133	35.0	32.0	35.0	7.0	35.0
90-91	30.074965949924913	34.0	31.0	35.0	4.5	35.0
92-93	29.925680475735064	34.0	31.0	35.0	2.0	35.0
94-95	29.82922867000282	34.0	31.0	35.0	2.0	35.0
96-97	29.634915469195953	34.0	31.0	35.0	2.0	35.0
98-99	29.441084191461762	34.0	31.0	35.0	2.0	35.0
100-101	28.329624153471112	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48326.0
3	16750.0
4	9394.0
5	6755.0
6	5977.0
7	6111.0
8	6271.0
9	6924.0
10	7678.0
11	8258.0
12	8831.0
13	9210.0
14	9994.0
15	10893.0
16	11559.0
17	12232.0
18	12023.0
19	10783.0
20	10301.0
21	10148.0
22	10828.0
23	12311.0
24	14604.0
25	17455.0
26	20965.0
27	25603.0
28	30708.0
29	37403.0
30	45588.0
31	56801.0
32	72051.0
33	95456.0
34	137691.0
35	205833.0
36	360196.0
37	693229.0
38	679233.0
39	61054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42663731354688	18.798195397234686	13.887308012816163	25.887859276402274
2	15.356153953638248	20.941172360890505	37.05981572786353	26.642857957607713
3	18.33346107707582	24.5969566093127	28.379430999663114	28.69015131394837
4	13.71961261565707	16.953732894731214	34.61160327896435	34.71505121064737
5	15.01435974778186	37.0496111013062	33.173245766503904	14.76278338440804
6	33.995977722772274	34.583421259752704	17.095034676278686	14.325566341196334
7	29.75846457598077	31.390693823079342	20.31330702955379	18.537534571386104
8	27.47100530507477	33.53304149421817	19.71446770848074	19.281485492226317
9	27.26882574381725	15.869562815214938	18.61210432493877	38.249507116029044
10-11	26.043970622534374	25.041404338147554	27.205788476256167	21.708836563061908
12-13	26.983504605995645	22.2556555267403	26.92458293670519	23.836256930558868
14-15	24.12892649009184	23.699630180639176	24.324931286033504	27.84651204323548
16-17	24.38831036712428	26.689743375872325	25.055553060390828	23.866393196612563
18-19	24.32615381544371	26.164170517589724	26.294847628919776	23.214828038046786
20-21	24.964189783720016	25.26582079047499	26.105179822803372	23.664809603001626
22-23	24.747569621308983	24.894606061751027	26.026002458805735	24.331821858134255
24-25	24.55733774575713	25.221304393080228	25.847219588424085	24.37413827273855
26-27	24.27343523591811	25.41029508166335	25.9230280331323	24.39324164928624
28-29	24.475748827125354	25.792013437484297	25.470762884510567	24.261474850879786
30-31	24.90586399066804	25.368148267512396	25.353335348793433	24.37265239302613
32-33	24.533255095416628	25.385037141026817	25.3265547736121	24.755152989944452
34-35	24.807982331041295	25.473954916064066	25.39884294771875	24.31921980517588
36-37	24.917032578837762	25.367462797443995	25.466774176759998	24.24873044695824
38-39	24.827900499264548	25.471296432111124	25.312728741957773	24.38807432666655
40-41	24.953494883768844	25.26483008366376	25.403583491677377	24.378091540890015
42-43	24.817921049718723	25.262296890688106	25.173353990425895	24.746428069167276
44-45	24.67397099029368	25.252318568133454	25.356926615912723	24.716783825660148
46-47	24.76705957336229	25.213103195309216	25.233760294239787	24.78607693708871
48-49	24.556653240396304	25.345973919122255	25.308169087589928	24.78920375289151
50-51	24.65310094444375	25.38208176861752	25.37202956488573	24.592787722053004
52-53	24.971853093913975	25.09376212518097	25.268231058963515	24.66615372194154
54-55	24.31950673684766	25.002227911416817	25.614796611293265	25.063468740442257
56-57	24.439307141405752	25.102488601485106	25.491820518396256	24.966383738712885
58-59	24.614061969693946	25.062816757600043	25.394636832153054	24.92848444055296
60-61	24.58750057036606	25.075323967922614	25.59171914034427	24.745456321367055
62-63	24.539317902957233	25.10145425640826	25.543423134797035	24.815804705837472
64-65	24.248948924318984	24.875261654548243	25.525109921655194	25.35067949947758
66-67	24.345469768586508	25.139318002066872	25.379550048640258	25.13566218070636
68-69	24.435903530628085	25.06763950821312	25.388250687985586	25.108206273173206
70-71	24.304703332419606	24.888430974696753	25.42441529591139	25.38245039697225
72-73	24.270343416046526	25.12310703983284	25.3211480244039	25.28540151971674
74-75	24.268572879221466	25.188739858820377	25.292745557919087	25.24994170403907
76-77	24.104629261781795	24.707830903473447	25.572691442567947	25.614848392176814
78-79	24.04474507894926	24.945679405658403	25.556356869671067	25.45321864572127
80-81	24.326646179615594	25.056325717360377	25.27469097766607	25.34233712535796
82-83	24.184939713608216	25.111470949452062	25.389972276442606	25.313617060497112
84-85	24.098045617649625	25.071358377848917	25.438033538393007	25.392562466108448
86-87	23.942497442953563	25.231338019691265	25.304156995071388	25.52200754228378
88-89	23.82736934831191	24.94772359447166	25.427175722726446	25.79773133448999
90-91	23.77553962595896	24.951543554314032	25.499986682633207	25.7729301370938
92-93	23.910232976987615	24.797507065987855	25.481601683354583	25.810658273669944
94-95	23.625604826563208	24.8193051120745	25.544918743892914	26.01017131746938
96-97	23.596440942142138	24.704225864274377	25.608000963988747	26.091332229594737
98-99	23.26611134607409	24.592888766743787	25.642879204849635	26.498120682332488
100-101	23.32340612100862	24.684467922806565	25.727074733907934	26.26505122227688
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	48.0
2	50.5
3	72.0
4	65.0
5	81.0
6	160.0
7	362.0
8	520.5
9	511.5
10	427.0
11	345.0
12	329.0
13	348.5
14	394.5
15	434.5
16	494.0
17	529.5
18	582.0
19	679.5
20	750.5
21	854.0
22	984.0
23	1133.5
24	1296.5
25	1507.0
26	1748.5
27	2124.5
28	2644.0
29	3189.0
30	3899.5
31	4991.5
32	7206.0
33	9898.5
34	13387.5
35	19314.0
36	27779.0
37	37344.5
38	47891.5
39	58188.5
40	66933.0
41	75646.5
42	85671.5
43	96246.5
44	106137.5
45	116009.0
46	123535.0
47	129600.5
48	135718.5
49	139270.0
50	138943.0
51	137569.5
52	135944.5
53	133857.0
54	132383.5
55	125646.0
56	114163.0
57	101661.5
58	92036.0
59	81032.5
60	65070.0
61	53162.5
62	44107.5
63	33267.0
64	23621.0
65	17233.5
66	12681.0
67	9780.0
68	7402.5
69	5335.5
70	3995.0
71	2849.5
72	1954.5
73	1433.5
74	1025.5
75	721.5
76	496.5
77	271.0
78	150.0
79	98.5
80	53.0
81	28.5
82	19.5
83	15.5
84	17.0
85	11.5
86	6.0
87	3.5
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1635758121669179
2	3.9209717451211526E-4
3	0.011834205630729297
4	0.005382424850120855
5	0.025379380750238732
6	0.001817905081828898
7	0.0
8	0.0
9	0.0
10-11	0.04894085641864857
12-13	2.6733898262189677E-4
14-15	0.00493685987908436
16-17	3.564519768291957E-5
18-19	0.0013901627096338633
20-21	4.455649710364946E-4
22-23	0.0
24-25	5.346779652437935E-4
26-27	0.0024238734424385307
28-29	0.00499032767560874
30-31	0.01579082257353337
32-33	0.010978720886339229
34-35	0.01003412314774186
36-37	0.0038853265474382333
38-39	1.7822598841459784E-5
40-41	0.003225890390304221
42-43	0.00914299320566887
44-45	0.006897345751644937
46-47	0.003421938977560278
48-49	0.008198395467071502
50-51	0.002566454233170209
52-53	0.0020139536690849556
54-55	0.0038853265474382333
56-57	0.008447911850851938
58-59	0.01579082257353337
60-61	0.00752113671109603
62-63	0.021084134429446927
64-65	0.04124149371913794
66-67	0.05986610950846342
68-69	0.05017061573870929
70-71	0.0674941818126082
72-73	0.03411245418255403
74-75	0.02755373780889683
76-77	0.22681039285641721
78-79	0.08528113545638508
80-81	0.0665674066728523
82-83	0.02753591521005537
84-85	0.05156077844834316
86-87	0.06770805299870572
88-89	0.29499965602384237
90-91	0.29683538370451273
92-93	0.05353908691974519
94-95	0.010123236141949158
96-97	0.014792757038411623
98-99	7.307265524998511E-4
100-101	2.49516383780437E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2805427.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.864317076870634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89408090561682	45.07287795934026
2	12.006042805213596	13.894429354387217
3	3.940699636341829	6.840776797859771
4	1.8830181218630484	4.358382306599074
5	1.0441872962221765	3.021059239812013
6	0.6769315708280197	2.350210983224198
7	0.4741543918882872	1.9205634052930236
8	0.34327211555487946	1.5890565230492575
9	0.25692351732011964	1.338003348364464
>10	1.3964755947087666	14.252342985679725
>50	0.06360898814870732	2.4806787422155376
>100	0.018690686558128975	1.8214426033610873
>500	0.0014203388361711644	0.5679831999790066
>1k	4.94030899456137E-4	0.49219255083541413
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA	3506	0.12497206307631602	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	3.564519768291957E-5
10-11	0.0	0.0	0.0	0.0	3.564519768291957E-5
12-13	0.0	0.0	0.0	3.564519768291957E-5	3.564519768291957E-5
14-15	0.0	0.0	0.0	3.564519768291957E-5	7.129039536583914E-5
16-17	0.0	0.0	0.0	5.3467796524379353E-5	1.7822598841459784E-4
18-19	3.564519768291957E-5	0.0	0.0	7.129039536583914E-5	1.7822598841459784E-4
20-21	3.564519768291957E-5	0.0	0.0	1.0693559304875871E-4	1.7822598841459784E-4
22-23	3.564519768291957E-5	0.0	0.0	1.9604858725605763E-4	2.49516383780437E-4
24-25	3.564519768291957E-5	0.0	0.0	5.168553664023337E-4	3.208067791462761E-4
26-27	3.564519768291957E-5	0.0	0.0	7.841943490242305E-4	3.208067791462761E-4
28-29	3.564519768291957E-5	0.0	0.0	0.002085244064450795	3.208067791462761E-4
30-31	3.564519768291957E-5	0.0	0.0	0.008768718629998215	3.208067791462761E-4
32-33	3.564519768291957E-5	0.0	0.0	0.027945834983408944	3.208067791462761E-4
34-35	3.564519768291957E-5	0.0	0.0	0.05494707222822052	4.2774237219503483E-4
36-37	3.564519768291957E-5	0.0	0.0	0.09445977385973686	4.455649710364946E-4
38-39	3.564519768291957E-5	0.0	0.0	0.16403919973679587	4.633875698779544E-4
40-41	3.564519768291957E-5	0.0	0.0	0.24841138265226648	4.633875698779544E-4
42-43	3.564519768291957E-5	0.0	0.0	0.3432810762853569	4.633875698779544E-4
44-45	3.564519768291957E-5	0.0	0.0	0.46014385688880877	4.812101687194142E-4
46-47	3.564519768291957E-5	0.0	0.0	0.5981798849159148	5.703231629267131E-4
48-49	3.564519768291957E-5	0.0	0.0	0.7394774485310079	6.772587559754718E-4
50-51	3.564519768291957E-5	1.7822598841459784E-5	0.0	0.8948548652308543	6.772587559754718E-4
52-53	3.564519768291957E-5	3.564519768291957E-5	0.0	1.056755353106675	7.48549151341311E-4
54-55	3.564519768291957E-5	3.564519768291957E-5	0.0	1.2399360239991988	8.198395467071501E-4
56-57	3.564519768291957E-5	3.564519768291957E-5	0.0	1.4593678609352516	8.376621455486099E-4
58-59	3.564519768291957E-5	3.564519768291957E-5	0.0	1.680991877528804	8.554847443900697E-4
60-61	3.564519768291957E-5	3.564519768291957E-5	0.0	1.92170389748156	8.554847443900697E-4
62-63	3.564519768291957E-5	3.564519768291957E-5	0.0	2.171024232674741	8.554847443900697E-4
64-65	3.564519768291957E-5	3.564519768291957E-5	0.0	2.4452070932517582	8.554847443900697E-4
66-67	3.564519768291957E-5	3.564519768291957E-5	0.0	2.773837993289435	8.733073432315294E-4
68-69	3.564519768291957E-5	3.564519768291957E-5	0.0	3.1292740819846676	8.911299420729892E-4
70-71	3.564519768291957E-5	3.564519768291957E-5	0.0	3.5089845503019683	8.911299420729892E-4
72-73	3.564519768291957E-5	3.564519768291957E-5	0.0	3.9155893202710317	9.624203374388284E-4
74-75	3.564519768291957E-5	3.564519768291957E-5	0.0	4.342101933146006	9.624203374388284E-4
76-77	3.564519768291957E-5	3.564519768291957E-5	0.0	4.82343686005731	9.624203374388284E-4
78-79	3.564519768291957E-5	3.564519768291957E-5	0.0	5.353694820788422	9.624203374388284E-4
80-81	3.564519768291957E-5	5.3467796524379353E-5	0.0	5.900474330645567	0.0010158881339632077
82-83	3.564519768291957E-5	7.129039536583914E-5	0.0	6.476964112771425	0.0012119367212192653
84-85	3.564519768291957E-5	7.129039536583914E-5	0.0	7.092788370540385	0.0012119367212192653
86-87	3.564519768291957E-5	7.129039536583914E-5	0.0	7.746432183050922	0.0012119367212192653
88-89	3.564519768291957E-5	7.129039536583914E-5	0.0	8.45270612994029	0.001247581918902185
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4450	0.0	47.43538	1
GGTATCA	2075	0.0	46.969414	1
TATCAAC	5770	0.0	36.526203	2
TCAACGC	5735	0.0	36.416744	4
ATCAACG	5980	0.0	35.16413	3
CAACGCA	5975	0.0	35.141685	5
AACGCAG	6385	0.0	32.95189	6
GTACATG	8965	0.0	27.151825	1
ACGCAGA	7805	0.0	26.591219	7
TACATGG	9605	0.0	25.500557	2
CGCAGAG	8155	0.0	25.158777	8
ACATGGG	10010	0.0	23.425573	3
CATGGGG	8455	0.0	21.389137	4
GCAGAGT	9160	0.0	20.9993	9
TATACCG	190	8.250878E-6	20.002054	5
GTGGTAT	1155	0.0	19.346182	1
ATGGGGG	4970	0.0	19.212236	5
TAAGGTA	690	0.0	18.57362	4
AGAGTAC	7675	0.0	18.471886	10-11
TAAGGCG	545	0.0	17.432983	5
>>END_MODULE