FastQCFastQC Report
Mon 15 Aug 2016
SRR2921701_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921701_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3505354
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.043.6112981
GTATCAA38500.042.651781
TCAACGC46950.034.2687534
CAACGCA48350.033.077335
ATCAACG49000.032.8369373
TATCAAC51750.031.3671262
AACGCAG52650.030.7228056
GTACATG91850.028.1087511
TACATGG95900.026.2310852
ACGCAGA63000.024.8511457
ACATGGG99050.024.7260193
CGCAGAG67750.022.9003688
CATGGGG85750.022.2498024
GTGTAGC22650.019.0675541
GCAGAGT81750.018.0497199
GTATATG11450.017.8231641
TATACCG2956.4157575E-817.6956965
AGAGTAC65850.017.29256810-11
GAGTACA68100.017.2832871
GTATAGC12050.016.9357031
Produced by FastQC (version 0.11.5)