##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921701_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3505354
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59449944285228	31.0	31.0	34.0	27.0	34.0
2	30.650532870574555	33.0	31.0	34.0	27.0	34.0
3	30.71622295494264	33.0	31.0	34.0	27.0	34.0
4	34.174843681979056	37.0	35.0	37.0	30.0	37.0
5	34.16938717173786	37.0	35.0	37.0	30.0	37.0
6	34.217075365284074	37.0	35.0	37.0	30.0	37.0
7	34.13947863753561	37.0	35.0	37.0	30.0	37.0
8	34.10807210912222	37.0	35.0	37.0	30.0	37.0
9	35.68570877577557	39.0	37.0	39.0	30.0	39.0
10-11	35.59067486479255	39.0	37.0	39.0	30.0	39.0
12-13	35.48674299371761	39.0	37.0	39.0	30.0	39.0
14-15	36.6450625243556	40.0	37.0	41.0	30.0	41.0
16-17	36.58540164559699	40.0	37.0	41.0	30.0	41.0
18-19	36.54294430747936	40.0	37.0	41.0	29.0	41.0
20-21	36.46796158105572	40.0	37.0	41.0	29.0	41.0
22-23	36.366649559502406	40.0	37.0	41.0	29.0	41.0
24-25	36.25463690685734	40.0	36.5	41.0	28.0	41.0
26-27	36.11381375461651	40.0	36.0	41.0	27.0	41.0
28-29	35.974396166549795	40.0	36.0	41.0	27.0	41.0
30-31	35.83503634725622	40.0	36.0	41.0	27.0	41.0
32-33	35.66403122195362	40.0	36.0	41.0	26.5	41.0
34-35	35.49650177984877	39.0	35.0	41.0	25.5	41.0
36-37	35.47307304768648	39.0	35.0	41.0	25.5	41.0
38-39	35.666881433373064	40.0	35.5	41.0	26.5	41.0
40-41	35.73299201735402	40.0	36.0	41.0	26.5	41.0
42-43	35.57655931469404	40.0	35.0	41.0	26.0	41.0
44-45	35.4609042339233	40.0	35.0	41.0	25.0	41.0
46-47	35.30865256404917	40.0	35.0	41.0	24.5	41.0
48-49	35.14232328603616	39.5	35.0	41.0	24.0	41.0
50-51	33.42196950721667	37.0	32.5	39.5	22.0	40.0
52-53	33.53917920986012	37.5	33.5	39.5	21.5	40.0
54-55	34.456126685065186	39.0	34.0	40.5	23.0	41.0
56-57	34.46895991103894	39.0	34.0	41.0	23.0	41.0
58-59	34.26690585316062	38.0	34.0	41.0	22.0	41.0
60-61	34.00251829059205	38.0	34.0	40.0	22.0	41.0
62-63	33.69701134321954	37.0	34.0	40.0	20.5	41.0
64-65	33.39166857327391	37.0	34.0	40.0	20.0	41.0
66-67	33.034947112331594	36.0	33.0	39.0	19.0	41.0
68-69	32.659451513313634	36.0	33.0	39.0	18.0	41.0
70-71	32.24061079137799	35.0	33.0	38.5	14.0	40.0
72-73	31.873059040541982	35.0	32.5	37.0	12.0	39.5
74-75	31.47381990520786	35.0	32.0	37.0	7.5	39.0
76-77	31.099962086568148	35.0	32.0	36.0	6.5	39.0
78-79	30.767730876824423	35.0	32.0	36.0	2.0	37.5
80-81	30.466627621632508	35.0	31.5	35.5	2.0	37.0
82-83	30.1779396603025	35.0	31.0	35.0	2.0	36.5
84-85	29.940357664304376	35.0	31.0	35.0	2.0	36.0
86-87	29.731898975110646	35.0	31.0	35.0	2.0	36.0
88-89	29.53169280477806	35.0	31.0	35.0	2.0	35.5
90-91	29.331918830451933	34.5	31.0	35.0	2.0	35.0
92-93	29.158684258422973	34.0	31.0	35.0	2.0	35.0
94-95	28.94907775363059	34.0	30.0	35.0	2.0	35.0
96-97	28.73107409408579	34.0	30.0	35.0	2.0	35.0
98-99	28.477470891670286	34.0	30.0	35.0	2.0	35.0
100-101	27.40606426626241	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101682.0
3	18296.0
4	13190.0
5	10153.0
6	9369.0
7	9657.0
8	10291.0
9	11032.0
10	11935.0
11	12685.0
12	12956.0
13	12845.0
14	13663.0
15	14230.0
16	14247.0
17	14925.0
18	14386.0
19	13901.0
20	14243.0
21	15076.0
22	16048.0
23	18674.0
24	21830.0
25	26402.0
26	31215.0
27	37116.0
28	44021.0
29	51753.0
30	62665.0
31	76498.0
32	95082.0
33	123289.0
34	171109.0
35	253254.0
36	430292.0
37	788240.0
38	803290.0
39	105803.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91249933280224	17.079092405534073	12.565638701637438	25.442769560026246
2	16.54916810792015	19.263958651774697	37.907659665096276	26.279213575208882
3	19.887914319058506	22.70946907323624	28.35292115919124	29.04969544851401
4	13.88965651121363	15.349226921847164	35.444776869318964	35.31633969762024
5	15.296069581283072	36.07153959836267	33.63035400117214	15.002036819182127
6	34.7003843092277	33.89781425909917	16.962273786801106	14.439527644872024
7	30.36069302546404	30.40591589288486	20.250257927269075	18.983133154382024
8	27.538689403672876	33.212355386414686	19.701401318374295	19.547553891538136
9	27.027457281477503	15.55024401210332	18.61173574684593	38.81056295957325
10-11	26.18959442972851	24.555492722742954	27.31156908686487	21.943343760663666
12-13	27.021681056596968	21.78687665749569	26.909911560281625	24.281530725625718
14-15	24.083231495534452	23.33214800033562	24.15196844236399	28.432652061765936
16-17	24.531369470804663	26.262756690793097	24.917699574518963	24.288174263883278
18-19	24.440958626052446	25.835011771056482	25.920304319934218	23.803725282956854
20-21	25.003923376877584	25.159503103605115	25.7962029701247	24.040370549392602
22-23	24.866714468666505	24.890858045897055	25.580947692483313	24.661479792953127
24-25	24.69897440046368	25.01532827332655	25.624249906289563	24.661447419920208
26-27	24.518687395406367	25.22316874653138	25.569495824814855	24.6886480332474
28-29	24.675581268949927	25.336216294560764	25.403349709169376	24.584852727319934
30-31	24.889966725749563	25.19429237830647	25.236660877256178	24.679080018687788
32-33	24.61833111892012	25.263967849972623	25.244852666154454	24.872848364952794
34-35	24.954752731953587	25.20493931274364	25.26532130695564	24.57498664834713
36-37	24.959301316322307	25.05708085776122	25.321049959711406	24.662567866205066
38-39	24.76495829516417	25.242744482600138	25.31346739399908	24.678829828236616
40-41	24.99501828151609	25.030735455699936	25.40722071744449	24.567025545339487
42-43	24.85447514832712	24.997733881319434	25.26480704821529	24.882983922138163
44-45	24.76835166895853	25.070789077207156	25.366208679810686	24.794650574023628
46-47	24.912936809647935	25.044982410119516	25.213848693294576	24.828232086937977
48-49	24.685683474052446	25.070645455304387	25.27870790005455	24.964963170588618
50-51	24.70098753407785	25.197920616776777	25.347046281270757	24.75404556787462
52-53	24.985544387984767	24.991905572011248	25.30940727630825	24.713142763695735
54-55	24.57573951750738	24.92039956665917	25.57078245635752	24.93307845947593
56-57	24.611268461692624	25.038087671544613	25.51912588615124	24.831517980611522
58-59	24.70896824272444	24.913170456668716	25.447392840656658	24.930468459950188
60-61	24.580809507579485	24.960970292817162	25.621458733161468	24.836761466441885
62-63	24.592490393502604	25.01686014981019	25.543219875507912	24.8474295811793
64-65	24.51872847545045	24.838878119715126	25.49115603939569	25.151237365438732
66-67	24.481597013818124	24.97350969995956	25.495539781047828	25.049353505174487
68-69	24.579230117681497	24.847813328736645	25.51561944799154	25.05733710559032
70-71	24.46710808018847	24.717175395559988	25.589602619211092	25.22611390504045
72-73	24.44631438236851	24.760233348051113	25.613940849387934	25.17951142019244
74-75	24.410561017611986	24.899444608344254	25.499818185560503	25.190176188483253
76-77	24.39035092295451	24.64357366363439	25.64148593739794	25.32458947601316
78-79	24.330659100418753	24.71052969024672	25.669448114778014	25.28936309455651
80-81	24.43841365009396	24.76329878795712	25.61893163629792	25.179355925651
82-83	24.3837855751368	24.699659535507728	25.707728375272104	25.20882651408337
84-85	24.279632951871932	24.740108601344886	25.723076148891032	25.25718229789215
86-87	24.247146315411683	24.77731751503877	25.719852035935766	25.25568413361378
88-89	24.191820428782442	24.58046322870346	25.763949326881026	25.46376701563307
90-91	24.13719341743844	24.616958575160538	25.836210951966287	25.40963705543473
92-93	24.184544940982285	24.472252101361796	25.955898532968007	25.387304424687912
94-95	24.072401942595768	24.37951967010474	25.997110270835254	25.550968116464233
96-97	24.083799280509492	24.307885768084283	26.061940588124433	25.54637436328179
98-99	23.99094957407405	24.17441458774921	26.029654672259504	25.80498116591723
100-101	24.110414490471932	24.11807898576992	26.200948481293125	25.57055804246502
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	21.5
2	49.5
3	80.0
4	70.0
5	78.5
6	120.5
7	218.0
8	353.5
9	422.0
10	393.5
11	380.0
12	443.5
13	501.0
14	577.5
15	714.0
16	839.0
17	927.0
18	1067.5
19	1220.5
20	1271.0
21	1356.0
22	1455.0
23	1492.0
24	1639.5
25	1861.5
26	2056.5
27	2369.5
28	2810.5
29	3397.5
30	4197.5
31	5383.0
32	7787.5
33	10942.0
34	15186.0
35	22142.5
36	32124.5
37	43997.5
38	56679.5
39	69610.0
40	82329.0
41	95425.0
42	108391.5
43	120702.0
44	131975.5
45	142487.5
46	152089.5
47	160543.5
48	167655.5
49	172312.5
50	172346.5
51	170877.5
52	169687.5
53	167296.5
54	163953.5
55	155706.0
56	143222.0
57	128813.0
58	116634.0
59	102647.5
60	84450.0
61	69713.0
62	57468.5
63	44455.0
64	32156.0
65	23481.5
66	17757.0
67	13611.5
68	10425.0
69	7935.5
70	5945.0
71	4345.5
72	3183.0
73	2383.0
74	1819.0
75	1349.5
76	954.0
77	616.0
78	400.0
79	276.5
80	208.0
81	177.5
82	150.5
83	118.0
84	99.5
85	79.5
86	63.0
87	65.5
88	57.5
89	42.5
90	38.0
91	31.5
92	26.5
93	25.5
94	21.0
95	17.0
96	15.0
97	11.5
98	13.0
99	11.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05406016054298653
2	0.07462869655960568
3	0.07126241743344609
4	0.07057775049253227
5	0.06684061010671105
6	0.010384115270526172
7	0.013978616710323693
8	0.01663170110636472
9	0.005705557840948446
10-11	0.013179838612590911
12-13	0.02339278714788863
14-15	0.040395349513915
16-17	0.02760063605558811
18-19	0.01022721242990009
20-21	0.02054000822741441
22-23	0.037556834488043145
24-25	0.034475833253930985
26-27	0.02051148043820966
28-29	0.011211421157463696
30-31	0.011168629473656584
32-33	0.008187475501761021
34-35	0.004849724164806179
36-37	5.562918894924736E-4
38-39	0.003565973650592779
40-41	0.0010697920951778337
42-43	0.23349995464081516
44-45	0.20281831735111489
46-47	0.20882341697871315
48-49	0.21698236469126939
50-51	0.20818154172160647
52-53	0.21616932269893424
54-55	0.21151929305856126
56-57	0.21404400240318094
58-59	0.22367213125978144
60-61	0.22873581384362324
62-63	0.2128458352565818
64-65	0.21277451578356993
66-67	0.2104067092795763
68-69	0.20491510985766345
70-71	0.1967276343559024
72-73	0.20875209750570128
74-75	0.20773936098893295
76-77	0.2011351777880351
78-79	0.22010615760918867
80-81	0.2315885927640974
82-83	0.23030484224988404
84-85	0.22489882619558538
86-87	0.24416934780338875
88-89	0.2371372477644198
90-91	0.24359879201929394
92-93	0.2395050542684134
94-95	0.24210108308604494
96-97	0.24856262734091905
98-99	0.24254326381871844
100-101	0.24851983565711194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3505354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.49398447188248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.67976946040648	45.61985013584952
2	12.767953423030393	15.192328453749775
3	4.419834202237846	7.888606421887006
4	2.0457784333250277	4.8684604138060505
5	1.105784167842871	3.289375305544863
6	0.7040174571702653	2.5130882198893167
7	0.45459240164045545	1.8931859298963116
8	0.33414571243761365	1.5903727861687595
9	0.2388462642795261	1.2788924344391936
>10	1.1871274986230123	12.148534438689614
>50	0.0493032071420023	1.9580999568325936
>100	0.011742272152825141	1.1373589443964365
>500	8.651736896635575E-4	0.3684296465408063
>1k	2.403260220513668E-4	0.25341691230978414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.852778920474223E-5
3	0.0	0.0	0.0	0.0	2.852778920474223E-5
4	0.0	0.0	0.0	0.0	2.852778920474223E-5
5	0.0	0.0	0.0	0.0	2.852778920474223E-5
6	0.0	0.0	0.0	0.0	8.55833676142267E-5
7	0.0	0.0	0.0	0.0	8.55833676142267E-5
8	0.0	0.0	0.0	0.0	8.55833676142267E-5
9	0.0	0.0	0.0	0.0	2.2822231363793784E-4
10-11	0.0	0.0	0.0	2.852778920474223E-5	2.2822231363793784E-4
12-13	0.0	0.0	0.0	5.705557840948446E-5	2.2822231363793784E-4
14-15	0.0	0.0	0.0	7.131947301185558E-5	3.1380568125216455E-4
16-17	0.0	0.0	0.0	8.55833676142267E-5	3.70861259661649E-4
18-19	0.0	2.852778920474223E-5	0.0	8.55833676142267E-5	4.1365294346876235E-4
20-21	0.0	2.852778920474223E-5	0.0	8.55833676142267E-5	4.8497241648061797E-4
22-23	0.0	4.279168380711335E-5	0.0	1.4263894602371116E-4	5.562918894924736E-4
24-25	0.0	5.705557840948446E-5	0.0	3.2806957585453567E-4	6.418752571067002E-4
26-27	0.0	5.705557840948446E-5	0.0	6.418752571067002E-4	6.846669409138136E-4
28-29	0.0	5.705557840948446E-5	0.0	0.0021681119795604097	6.846669409138136E-4
30-31	0.0	5.705557840948446E-5	0.0	0.008857878548072464	6.846669409138136E-4
32-33	0.0	5.705557840948446E-5	0.0	0.025646482495063266	7.41722519323298E-4
34-35	0.0	5.705557840948446E-5	0.0	0.051435603936150244	7.845142031304114E-4
36-37	0.0	5.705557840948446E-5	0.0	0.0939277459566138	8.130419923351535E-4
38-39	0.0	8.55833676142267E-5	0.0	0.16402052403266545	8.273058869375247E-4
40-41	0.0	8.55833676142267E-5	0.0	0.24743577966733174	8.273058869375247E-4
42-43	0.0	8.55833676142267E-5	0.0	0.3372840517676674	8.70097570744638E-4
44-45	0.0	8.55833676142267E-5	0.0	0.4510243473269747	8.843614653470091E-4
46-47	0.0	8.55833676142267E-5	0.0	0.5839781089156759	8.843614653470091E-4
48-49	0.0	8.55833676142267E-5	0.0	0.7244917346436337	8.843614653470091E-4
50-51	1.4263894602371115E-5	8.55833676142267E-5	0.0	0.8761597259506457	9.128892545517514E-4
52-53	2.852778920474223E-5	8.55833676142267E-5	0.0	1.0340753030934964	9.556809383588648E-4
54-55	2.852778920474223E-5	1.5690284062608228E-4	0.0	1.2147417921271288	0.0010127365167683492
56-57	2.852778920474223E-5	1.9969452443319562E-4	0.0	1.4260899184504616	0.0010270004113707204
58-59	2.852778920474223E-5	1.9969452443319562E-4	0.0	1.6526148286307176	0.0010270004113707204
60-61	2.852778920474223E-5	1.9969452443319562E-4	0.0	1.8898804514465586	0.0010412643059730916
62-63	2.852778920474223E-5	1.9969452443319562E-4	0.0	2.136717147540591	0.0010840559897802048
64-65	2.852778920474223E-5	1.9969452443319562E-4	0.0	2.4068753113095	0.0010840559897802048
66-67	2.852778920474223E-5	1.9969452443319562E-4	0.0	2.7265719810324436	0.001112583778984947
68-69	2.852778920474223E-5	1.9969452443319562E-4	0.0	3.0728137586104	0.001112583778984947
70-71	2.852778920474223E-5	2.1395841903556673E-4	0.0	3.434303639518291	0.0011268476735873181
72-73	4.279168380711335E-5	2.2822231363793784E-4	0.0	3.8148072919311433	0.0011981671465991738
74-75	5.705557840948446E-5	2.2822231363793784E-4	0.0	4.215037910579074	0.0012266949358039159
76-77	5.705557840948446E-5	2.2822231363793784E-4	0.0	4.663951201504898	0.0012980144088157715
78-79	5.705557840948446E-5	2.852778920474223E-4	0.0	5.15370202267731	0.0013122783034181427
80-81	5.705557840948446E-5	2.852778920474223E-4	0.0	5.666004061216071	0.001355069987225256
82-83	5.705557840948446E-5	2.852778920474223E-4	0.0	6.199216969241908	0.0014406533548394827
84-85	5.705557840948446E-5	2.852778920474223E-4	0.0	6.764209834441829	0.001454917249441854
86-87	5.705557840948446E-5	3.1380568125216455E-4	0.0	7.361524684810721	0.0014977089332489672
88-89	5.705557840948446E-5	3.1380568125216455E-4	0.0	8.007878804822566	0.0015405006170560805
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2220	0.0	43.611298	1
GTATCAA	3850	0.0	42.65178	1
TCAACGC	4695	0.0	34.268753	4
CAACGCA	4835	0.0	33.07733	5
ATCAACG	4900	0.0	32.836937	3
TATCAAC	5175	0.0	31.367126	2
AACGCAG	5265	0.0	30.722805	6
GTACATG	9185	0.0	28.108751	1
TACATGG	9590	0.0	26.231085	2
ACGCAGA	6300	0.0	24.851145	7
ACATGGG	9905	0.0	24.726019	3
CGCAGAG	6775	0.0	22.900368	8
CATGGGG	8575	0.0	22.249802	4
GTGTAGC	2265	0.0	19.067554	1
GCAGAGT	8175	0.0	18.049719	9
GTATATG	1145	0.0	17.823164	1
TATACCG	295	6.4157575E-8	17.695696	5
AGAGTAC	6585	0.0	17.292568	10-11
GAGTACA	6810	0.0	17.283287	1
GTATAGC	1205	0.0	16.935703	1
>>END_MODULE