FastQCFastQC Report
Mon 15 Aug 2016
SRR2921701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3505354
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39050.041.5167541
GGTATCA23650.040.6078761
TCAACGC47850.033.426724
CAACGCA50250.031.8565855
ATCAACG50650.031.5779443
TATCAAC51550.031.1186982
AACGCAG54500.029.5432956
GTACATG88900.026.7932871
TACATGG92000.025.6390512
ACGCAGA66450.023.5864357
ACATGGG96750.023.1048583
CGCAGAG70950.021.6218818
GTATAGT7400.020.55931
CATGGGG82800.020.177074
GTATACG2704.8339825E-717.608661
TAAGGTG16900.017.4265065
GTATAGA9350.017.2885021
GAGTACA69350.017.276041
AGAGTAC65850.017.09393710-11
AGTACAT65250.017.0930962
Produced by FastQC (version 0.11.5)