##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3505354
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462123654272865	33.0	31.0	34.0	29.0	34.0
2	31.602240458452982	34.0	31.0	34.0	29.0	34.0
3	31.6743039362073	34.0	31.0	34.0	28.0	34.0
4	35.15200005477335	37.0	35.0	37.0	33.0	37.0
5	34.9049884833315	37.0	35.0	37.0	32.0	37.0
6	34.87752107205149	37.0	35.0	37.0	32.0	37.0
7	34.77087135849903	37.0	35.0	37.0	32.0	37.0
8	34.711032323696834	37.0	35.0	37.0	32.0	37.0
9	36.31400851383341	39.0	37.0	39.0	32.0	39.0
10-11	36.20804988597443	39.0	37.0	39.0	32.0	39.0
12-13	36.134247639468086	39.0	37.0	39.0	32.0	39.0
14-15	37.38909151543611	40.0	38.0	41.0	32.0	41.0
16-17	37.357422531362026	40.0	38.0	41.0	32.0	41.0
18-19	37.30560294338318	40.0	38.0	41.0	31.0	41.0
20-21	37.2461956766706	40.0	38.0	41.0	31.0	41.0
22-23	37.15337709115827	40.0	38.0	41.0	31.0	41.0
24-25	37.07014854990395	40.0	37.5	41.0	31.0	41.0
26-27	36.944557382792155	40.0	37.0	41.0	30.0	41.0
28-29	36.82521565582249	40.0	37.0	41.0	30.0	41.0
30-31	36.67566428383553	40.0	37.0	41.0	30.0	41.0
32-33	36.6358143856512	40.0	37.0	41.0	30.0	41.0
34-35	36.77998712826151	40.0	37.0	41.0	30.0	41.0
36-37	36.78078447996978	40.0	37.0	41.0	30.0	41.0
38-39	36.679838184674075	40.0	37.0	41.0	30.0	41.0
40-41	36.602515181063026	40.0	37.0	41.0	30.0	41.0
42-43	36.486347598559234	40.0	37.0	41.0	30.0	41.0
44-45	36.360973813201184	40.0	36.0	41.0	29.5	41.0
46-47	36.20760428190705	40.0	36.0	41.0	29.0	41.0
48-49	36.06548397109108	40.0	36.0	41.0	28.5	41.0
50-51	35.90320135997676	40.0	35.0	41.0	28.0	41.0
52-53	35.7217596282715	39.0	35.0	41.0	28.0	41.0
54-55	35.49761764432351	39.0	35.0	41.0	27.5	41.0
56-57	35.26092386104228	39.0	35.0	41.0	27.0	41.0
58-59	35.00750380703347	38.5	35.0	40.5	26.5	41.0
60-61	34.72068170575639	38.0	34.5	40.0	26.0	41.0
62-63	34.40418057634122	37.0	34.0	40.0	26.0	41.0
64-65	34.057375945482256	37.0	34.0	40.0	26.0	41.0
66-67	33.68739562395125	36.0	34.0	39.0	25.5	41.0
68-69	33.30862546264942	36.0	34.0	39.0	25.0	40.5
70-71	32.89874817208191	35.0	33.0	38.5	24.0	40.0
72-73	32.5299210864295	35.0	33.0	37.0	23.0	39.5
74-75	32.12432125257534	35.0	33.0	37.0	22.5	39.0
76-77	29.429008596564	33.0	29.0	34.5	17.5	36.0
78-79	31.00178641016	34.5	31.5	35.5	19.5	37.0
80-81	31.186823927055585	35.0	32.0	35.0	20.0	37.0
82-83	31.026861766315186	35.0	32.0	35.0	20.0	36.5
84-85	30.84708249152582	35.0	32.0	35.0	19.0	36.0
86-87	30.65569040958488	35.0	32.0	35.0	18.0	36.0
88-89	30.402570610557447	35.0	32.0	35.0	13.0	35.5
90-91	30.21959137365299	34.5	32.0	35.0	9.0	35.0
92-93	30.073809378453646	34.0	31.0	35.0	6.5	35.0
94-95	29.981617833748032	34.0	31.0	35.0	2.0	35.0
96-97	29.783732684345146	34.0	31.0	35.0	2.0	35.0
98-99	29.586970531364308	34.0	31.0	35.0	2.0	35.0
100-101	28.472274840144532	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58033.0
3	20311.0
4	11449.0
5	8203.0
6	7161.0
7	7365.0
8	7660.0
9	8287.0
10	9219.0
11	10036.0
12	10336.0
13	11181.0
14	11747.0
15	12404.0
16	13333.0
17	14074.0
18	13948.0
19	12831.0
20	12316.0
21	12180.0
22	13041.0
23	15056.0
24	17583.0
25	21279.0
26	25583.0
27	31351.0
28	37853.0
29	45861.0
30	56517.0
31	69676.0
32	88722.0
33	116892.0
34	166706.0
35	251736.0
36	451068.0
37	875915.0
38	868976.0
39	79465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79720172012801	18.580531486292212	13.101845488521668	26.52042130505811
2	15.65483741856379	20.649658194001113	36.429418908406035	27.266085479029062
3	18.496983004453384	23.89758905693247	28.173366044912708	29.432061893701444
4	13.57255629852497	16.573308904544565	34.495945113966464	35.358189682964
5	15.044470259042933	36.44306982183076	33.65883126069881	14.853628658427493
6	34.624738644050105	34.54266239158845	16.684644323401546	14.1479546409599
7	30.13222059740614	30.708538994920342	20.32810380920158	18.831136598471936
8	27.540214198052464	33.67037965352429	19.46091607295583	19.328490075467414
9	26.881593128682578	15.500488681029076	18.534675813056257	39.08324237723208
10-11	26.152484926326302	24.81298654964501	27.277576795461844	21.756951728566843
12-13	26.9111412872931	21.9701797828456	26.93644553738011	24.182233392481187
14-15	23.97227299240024	23.545004678083785	24.093179954378876	28.3895423751371
16-17	24.479228699758814	26.523031129385384	24.83709560248346	24.160644568372348
18-19	24.428996397595355	26.094603953016826	25.82750822148477	23.648891427903052
20-21	25.00480694619415	25.320068444067118	25.6955522767523	23.979572332986436
22-23	24.78119539156322	25.02716202517093	25.546539029515852	24.64510355375
24-25	24.64400279631344	25.204848788109647	25.589734146247878	24.56141426932903
26-27	24.424859019459554	25.42752043993175	25.53806824065814	24.60955229995056
28-29	24.607330813821957	25.529980369037	25.3603455688543	24.50234324828675
30-31	24.848974223079466	25.378978581409832	25.195472186933145	24.576575008577564
32-33	24.612966283918546	25.387411751833366	25.169178131730195	24.830443832517894
34-35	24.8370825065371	25.402703855497656	25.232846266542463	24.527367371422784
36-37	24.887282203026693	25.242253397367463	25.301821800323747	24.568642599282096
38-39	24.794057889730965	25.356312087167233	25.29969868949042	24.549931333611383
40-41	24.962720088416223	25.18725541967324	25.33158224018391	24.51844225172663
42-43	24.791934674434934	25.171874469512233	25.203043969108386	24.833146886944448
44-45	24.73072954156478	25.199401218617684	25.28292192076788	24.786947319049652
46-47	24.807059846537978	25.21291030094442	25.153927043916063	24.82610280860154
48-49	24.64379201978862	25.23391283136165	25.249875536548032	24.872419612301698
50-51	24.64421418156495	25.36798174405445	25.245737203050606	24.74206687132999
52-53	24.922659663148742	25.14097280507211	25.201567004803604	24.734800526975544
54-55	24.536846051273447	25.10601374070454	25.4260662568762	24.93107395114581
56-57	24.552153780161746	25.190926373609283	25.373661068936627	24.88325877729234
58-59	24.601936282128886	25.094773339321296	25.308936191824404	24.994354186725413
60-61	24.508901595445725	25.14287743980061	25.478473950258795	24.86974701449487
62-63	24.550440720892748	25.212128406131484	25.320785630538616	24.916645242437152
64-65	24.3922747130201	25.009164663568733	25.326463938172587	25.272096685238576
66-67	24.36623461293855	25.158237965237028	25.30318833728964	25.172339084534784
68-69	24.5137049700264	25.08149514368576	25.275582168311693	25.12921771797614
70-71	24.372055792268164	24.939033676819182	25.322152688934224	25.366757841978433
72-73	24.34958323529609	25.05863328686998	25.270436538144065	25.321346939689864
74-75	24.363238737445013	25.08372608582814	25.171172459115006	25.381862717611845
76-77	24.236134968825205	24.843418337564458	25.383078043282865	25.537368650327473
78-79	24.20514242265994	24.925628655217043	25.36576082906454	25.503468093058473
80-81	24.293170909741775	25.018330604022843	25.23839776604283	25.45010072019255
82-83	24.23784861654851	24.96901400251769	25.32355345293232	25.469583928001484
84-85	24.073772262846223	24.90811433810213	25.351837099572744	25.666276299478895
86-87	24.054367769070442	24.990814808555488	25.309677266487025	25.64514015588704
88-89	23.961367267183796	24.832979996798297	25.309716079942994	25.895936656074912
90-91	23.843899747942032	24.879829668444625	25.38477540115674	25.8914951824566
92-93	23.898571770070323	24.735672209992256	25.436964146183954	25.928791873753465
94-95	23.711910084918784	24.683837014364638	25.469829352159035	26.134423548557546
96-97	23.697080021227908	24.59417316723826	25.520254963165012	26.188491848368816
98-99	23.517968667090404	24.495082337609876	25.464279478872708	26.522669516427012
100-101	23.4873499666866	24.518246626973966	25.565889135353665	26.428514270985765
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	19.5
2	28.0
3	46.5
4	49.0
5	49.0
6	102.5
7	220.5
8	325.0
9	342.5
10	280.5
11	244.0
12	247.0
13	273.0
14	311.5
15	349.0
16	385.5
17	420.0
18	457.5
19	502.5
20	583.5
21	684.5
22	771.5
23	921.0
24	1155.0
25	1445.5
26	1749.0
27	2185.5
28	2748.0
29	3363.0
30	4279.0
31	5680.0
32	8307.5
33	11604.0
34	16060.0
35	23279.0
36	33370.0
37	45155.5
38	57511.5
39	69932.5
40	82085.5
41	94509.0
42	107651.0
43	120187.0
44	131412.5
45	142619.5
46	152404.0
47	160557.5
48	168003.5
49	172923.5
50	173241.0
51	172184.5
52	171023.5
53	169724.5
54	166912.0
55	157911.5
56	144749.5
57	129624.0
58	116645.0
59	102104.0
60	84137.0
61	69446.5
62	56819.5
63	43606.5
64	31326.5
65	22730.5
66	16954.5
67	12980.0
68	9899.0
69	7332.5
70	5415.0
71	3801.0
72	2653.5
73	1984.5
74	1504.5
75	1071.5
76	716.5
77	429.5
78	229.5
79	135.5
80	91.5
81	49.0
82	31.5
83	20.0
84	13.5
85	11.0
86	11.5
87	7.5
88	3.5
89	2.5
90	2.0
91	1.5
92	1.0
93	2.0
94	2.5
95	1.5
96	1.5
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1668019834801278
2	8.55833676142267E-5
3	0.01075497653018782
4	0.005705557840948446
5	0.025018871132558934
6	0.0021966397687651516
7	0.0
8	0.0
9	0.0
10-11	0.04932454753499932
12-13	3.9938904886639123E-4
14-15	0.00512073816225123
16-17	1.4263894602371115E-5
18-19	0.0016118200900679359
20-21	2.852778920474223E-4
22-23	1.4263894602371115E-5
24-25	6.989308355161847E-4
26-27	0.002367806503993605
28-29	0.005049418689239375
30-31	0.016303631530510187
32-33	0.01125421284127081
34-35	0.01024147632450246
36-37	0.003993890488663912
38-39	0.0
40-41	0.003280695758545357
42-43	0.009271531491541226
44-45	0.006704030463114424
46-47	0.003480390282978552
48-49	0.00881508686426535
50-51	0.0024248620824030894
52-53	0.0019398896659224717
54-55	0.00422211280230185
56-57	0.007773822558292258
58-59	0.015533381221982143
60-61	0.0071890028795950425
62-63	0.021538480849580387
64-65	0.04012433551646995
66-67	0.0595089682810923
68-69	0.050522714681598496
70-71	0.06833831903996002
72-73	0.03304944379369387
74-75	0.026259829962965227
76-77	0.22986266151721052
78-79	0.08549778424661247
80-81	0.06654106832006126
82-83	0.02705860806069801
84-85	0.051777937406607154
86-87	0.06940811113513784
88-89	0.29493454869322755
90-91	0.3001979258015025
92-93	0.05396031328076993
94-95	0.010212948535297719
96-97	0.01523383943533235
98-99	8.558336761422669E-4
100-101	2.1395841903556673E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3505354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.61009697322345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8218269950869	44.43924632869014
2	13.238903125155307	15.51866791968927
3	4.605987862364415	8.098721858120054
4	2.080669400689711	4.877929413745707
5	1.15988042710197	3.3990352154895147
6	0.7284380819178194	2.5616295972115366
7	0.4650287961977468	1.9078767988343908
8	0.3301534748198384	1.548026174018993
9	0.26197749214119703	1.3819073597277698
>10	1.2437637397806918	12.53631174534775
>50	0.049926800176957505	1.9640552138998415
>100	0.012272511043741764	1.1484882978346607
>500	8.784701451207434E-4	0.35068517819910544
>1k	2.9282337871662956E-4	0.2674188991912792
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.55833676142267E-5
2	0.0	0.0	0.0	0.0	8.55833676142267E-5
3	0.0	0.0	0.0	0.0	8.55833676142267E-5
4	0.0	0.0	0.0	0.0	1.1411115681896892E-4
5	0.0	0.0	0.0	0.0	1.1411115681896892E-4
6	0.0	0.0	0.0	0.0	1.4263894602371116E-4
7	0.0	0.0	0.0	0.0	1.711667352284534E-4
8	0.0	0.0	0.0	0.0	1.711667352284534E-4
9	0.0	0.0	0.0	0.0	1.711667352284534E-4
10-11	0.0	0.0	0.0	0.0	1.9969452443319562E-4
12-13	0.0	0.0	0.0	4.279168380711335E-5	2.567501028426801E-4
14-15	0.0	0.0	0.0	7.131947301185558E-5	2.567501028426801E-4
16-17	0.0	0.0	0.0	8.55833676142267E-5	2.567501028426801E-4
18-19	0.0	0.0	0.0	8.55833676142267E-5	2.567501028426801E-4
20-21	0.0	0.0	0.0	9.984726221659781E-5	2.852778920474223E-4
22-23	0.0	0.0	0.0	1.711667352284534E-4	3.1380568125216455E-4
24-25	0.0	0.0	0.0	3.70861259661649E-4	3.70861259661649E-4
26-27	0.0	0.0	0.0	6.846669409138136E-4	4.1365294346876235E-4
28-29	0.0	0.0	0.0	0.002210903663367523	4.421807326735046E-4
30-31	0.0	0.0	0.0	0.00894346191568669	5.135002056853602E-4
32-33	0.0	0.0	0.0	0.026088663227736772	5.135002056853602E-4
34-35	0.0	0.0	0.0	0.05244834045291859	5.420279948901024E-4
36-37	0.0	0.0	0.0	0.0949119546841774	5.705557840948447E-4
38-39	0.0	0.0	0.0	0.16556102464972156	6.561391517090713E-4
40-41	0.0	0.0	0.0	0.2497322666983135	7.41722519323298E-4
42-43	0.0	0.0	0.0	0.3406788586830317	7.702503085280402E-4
44-45	0.0	0.0	0.0	0.45568864086195005	7.702503085280402E-4
46-47	0.0	0.0	0.0	0.5890132637103129	7.845142031304114E-4
48-49	0.0	0.0	0.0	0.7294127782814517	7.987780977327825E-4
50-51	0.0	0.0	0.0	0.8818224921077871	7.987780977327825E-4
52-53	0.0	0.0	0.0	1.0395669025154093	8.273058869375247E-4
54-55	0.0	0.0	0.0	1.2197912108163682	8.558336761422669E-4
56-57	0.0	0.0	0.0	1.4313390316641343	8.558336761422669E-4
58-59	0.0	0.0	0.0	1.6580351085796186	8.558336761422669E-4
60-61	0.0	0.0	0.0	1.895771439917338	8.558336761422669E-4
62-63	0.0	0.0	0.0	2.1425082887491533	9.128892545517514E-4
64-65	0.0	2.852778920474223E-5	0.0	2.4146633977623946	9.414170437564936E-4
66-67	0.0	2.852778920474223E-5	0.0	2.7346168175881806	9.414170437564936E-4
68-69	0.0	4.279168380711335E-5	0.0	3.0818285399990986	9.414170437564936E-4
70-71	0.0	7.131947301185558E-5	0.0	3.4439602961640965	9.699448329612358E-4
72-73	1.4263894602371115E-5	8.55833676142267E-5	0.0	3.8245495319445624	0.0010127365167683492
74-75	2.852778920474223E-5	8.55833676142267E-5	0.0	4.227205012674897	0.0010270004113707204
76-77	4.279168380711335E-5	8.55833676142267E-5	0.0	4.677116776222887	0.0010270004113707204
78-79	5.705557840948446E-5	8.55833676142267E-5	0.0	5.169363778950713	0.0010270004113707204
80-81	5.705557840948446E-5	8.55833676142267E-5	0.0	5.685930721975584	0.0010412643059730916
82-83	5.705557840948446E-5	8.55833676142267E-5	0.0	6.223807923536397	0.0011268476735873181
84-85	5.705557840948446E-5	8.55833676142267E-5	0.0	6.79249513743833	0.0011411115681896893
86-87	5.705557840948446E-5	8.55833676142267E-5	0.0	7.393989308925717	0.0011411115681896893
88-89	5.705557840948446E-5	8.55833676142267E-5	0.0	8.045934875621692	0.0011411115681896893
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3905	0.0	41.516754	1
GGTATCA	2365	0.0	40.607876	1
TCAACGC	4785	0.0	33.42672	4
CAACGCA	5025	0.0	31.856585	5
ATCAACG	5065	0.0	31.577944	3
TATCAAC	5155	0.0	31.118698	2
AACGCAG	5450	0.0	29.543295	6
GTACATG	8890	0.0	26.793287	1
TACATGG	9200	0.0	25.639051	2
ACGCAGA	6645	0.0	23.586435	7
ACATGGG	9675	0.0	23.104858	3
CGCAGAG	7095	0.0	21.621881	8
GTATAGT	740	0.0	20.5593	1
CATGGGG	8280	0.0	20.17707	4
GTATACG	270	4.8339825E-7	17.60866	1
TAAGGTG	1690	0.0	17.426506	5
GTATAGA	935	0.0	17.288502	1
GAGTACA	6935	0.0	17.27604	1
AGAGTAC	6585	0.0	17.093937	10-11
AGTACAT	6525	0.0	17.093096	2
>>END_MODULE