FastQCFastQC Report
Mon 15 Aug 2016
SRR2921700_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921700_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336315
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.048.1171721
GTATCAA40300.046.0477941
TCAACGC48850.036.2421534
TATCAAC52100.034.2557032
ATCAACG51550.034.252363
CAACGCA51650.034.181435
AACGCAG55350.032.2215846
GTACATG99950.028.06351
ACGCAGA65850.026.8716817
TACATGG105750.026.3026812
CGCAGAG70550.024.881278
ACATGGG108700.024.496813
CATGGGG98050.021.0093144
ATGGGGG58350.019.7643745
GCAGAGT83100.019.6963569
GAGTACA75550.019.097491
AGTACAT73650.018.6899452
GCGTAAA1851.5320309E-417.9582271
GTATATA8050.017.687341
GTATAGC11050.017.6099761
Produced by FastQC (version 0.11.5)