##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921700_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3336315
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.605152391186085	31.0	31.0	34.0	27.0	34.0
2	30.66145013285616	31.0	31.0	34.0	27.0	34.0
3	30.72722389822304	33.0	31.0	34.0	27.0	34.0
4	34.195367343910874	37.0	35.0	37.0	30.0	37.0
5	34.194168116619686	37.0	35.0	37.0	30.0	37.0
6	34.2375686948025	37.0	35.0	37.0	30.0	37.0
7	34.16233449179709	37.0	35.0	37.0	30.0	37.0
8	34.13058029592529	37.0	35.0	37.0	30.0	37.0
9	35.702149527247876	39.0	37.0	39.0	30.0	39.0
10-11	35.6048514603687	39.0	37.0	39.0	30.0	39.0
12-13	35.49964376864894	39.0	35.0	39.0	30.0	39.0
14-15	36.65128682393599	40.0	37.0	41.0	30.0	41.0
16-17	36.589198861618286	40.0	37.0	41.0	30.0	41.0
18-19	36.5519048710928	40.0	37.0	41.0	29.5	41.0
20-21	36.4735570532159	40.0	37.0	41.0	29.0	41.0
22-23	36.37487527406735	40.0	37.0	41.0	29.0	41.0
24-25	36.26411789654154	40.0	36.0	41.0	28.0	41.0
26-27	36.12099531968654	40.0	36.0	41.0	27.5	41.0
28-29	35.980014477050275	40.0	36.0	41.0	27.0	41.0
30-31	35.835827851986394	40.0	36.0	41.0	27.0	41.0
32-33	35.66227709314019	39.5	35.5	41.0	26.5	41.0
34-35	35.49662381999302	39.0	35.0	41.0	25.5	41.0
36-37	35.47110344796579	39.0	35.0	41.0	25.5	41.0
38-39	35.65989287582258	40.0	35.5	41.0	26.5	41.0
40-41	35.72797832338973	40.0	35.5	41.0	26.5	41.0
42-43	35.57118107852526	40.0	35.0	41.0	26.0	41.0
44-45	35.45928067343761	40.0	35.0	41.0	25.5	41.0
46-47	35.30382667703739	40.0	35.0	41.0	24.5	41.0
48-49	35.14375650980198	39.0	35.0	41.0	24.0	41.0
50-51	33.43094372084171	37.0	32.5	39.5	22.0	40.0
52-53	33.55685689151055	37.5	33.5	39.5	22.5	40.0
54-55	34.476824580412824	39.0	34.0	40.5	23.0	41.0
56-57	34.49230573252226	39.0	34.0	41.0	23.0	41.0
58-59	34.29549428036621	38.0	34.0	41.0	22.0	41.0
60-61	34.03225969370398	38.0	34.0	40.0	22.0	41.0
62-63	33.7288348072649	37.0	34.0	40.0	21.0	41.0
64-65	33.432018409532674	37.0	34.0	40.0	20.0	41.0
66-67	33.08309197422905	36.0	33.0	39.0	19.5	41.0
68-69	32.71484107465872	36.0	33.0	39.0	18.0	41.0
70-71	32.29514374392106	35.0	33.0	38.5	15.5	40.5
72-73	31.927333000630938	35.0	32.5	37.0	12.5	39.5
74-75	31.525257507159843	35.0	32.0	37.0	9.0	39.0
76-77	31.148959555677447	35.0	32.0	36.5	7.0	39.0
78-79	30.809324209494605	35.0	32.0	36.0	2.0	37.5
80-81	30.50188516372105	35.0	31.5	35.5	2.0	37.0
82-83	30.200235289533513	35.0	31.0	35.0	2.0	37.0
84-85	29.950397669284825	35.0	31.0	35.0	2.0	36.0
86-87	29.727543412417592	35.0	31.0	35.0	2.0	36.0
88-89	29.52229870380944	35.0	31.0	35.0	2.0	36.0
90-91	29.308121984884522	34.5	31.0	35.0	2.0	35.0
92-93	29.12412661873954	34.0	30.5	35.0	2.0	35.0
94-95	28.898029562556292	34.0	30.0	35.0	2.0	35.0
96-97	28.663162051544894	34.0	30.0	35.0	2.0	35.0
98-99	28.401593224860363	34.0	30.0	35.0	2.0	35.0
100-101	27.32448974392406	33.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94040.0
3	16937.0
4	12020.0
5	9508.0
6	8762.0
7	8925.0
8	9696.0
9	10290.0
10	11407.0
11	12171.0
12	12283.0
13	12384.0
14	13095.0
15	13639.0
16	13589.0
17	14159.0
18	13730.0
19	13204.0
20	13752.0
21	14660.0
22	15905.0
23	18282.0
24	21632.0
25	25461.0
26	30619.0
27	36344.0
28	42957.0
29	50959.0
30	60566.0
31	73993.0
32	92416.0
33	120499.0
34	168898.0
35	242709.0
36	402000.0
37	734613.0
38	759960.0
39	110244.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86448763637683	17.36596390555596	12.666644473894854	25.102903984172354
2	16.386213574293624	19.705456761890137	38.02821548684193	25.88011417697431
3	19.48209520747147	23.605908593532536	28.68615201136462	28.225844187631377
4	13.973655439058518	15.675778631300627	35.83805128835792	34.51251464128294
5	15.032472528148205	36.71826658816352	33.54989683714906	14.699364046539207
6	33.99336263621629	34.2320943008061	17.361733328577085	14.41280973440053
7	29.700801535802313	31.186211833519895	20.609194788968075	18.50379184170972
8	27.353689147879194	33.443255651231276	20.09683756489289	19.106217635996643
9	27.447609044409116	15.504341632914398	18.854748938272316	38.193300384404175
10-11	26.058818327480278	24.780903903485303	27.48694866249084	21.67332910654358
12-13	26.777209305044707	22.148461173742046	27.08423554707918	23.990093974134066
14-15	24.037873221291296	23.562306510541255	24.4328394705977	27.96698079756975
16-17	24.34689678909535	26.533481602118375	25.166577521508763	23.953044087277505
18-19	24.335521246458498	26.157429924254632	26.218266629036542	23.28878220025033
20-21	24.90818820324089	25.31715167278845	26.045979947404206	23.728680176566453
22-23	24.75183978137613	25.102944699663542	25.730492830227377	24.414722688732947
24-25	24.573668443803992	25.256248690084583	25.7283429566204	24.441739909491027
26-27	24.367970780542127	25.440705986999884	25.761639148509307	24.42968408394868
28-29	24.488875206202053	25.75405576920598	25.406731022591323	24.350338002000644
30-31	24.92784212545016	25.394640660004924	25.32926128652278	24.348255928022137
32-33	24.524877586715967	25.456777282012926	25.329680294121616	24.688664837149492
34-35	24.834790579364892	25.53153797744672	25.397790773151463	24.235880670036924
36-37	24.88884346246095	25.436038756869166	25.387122087255108	24.287995693414775
38-39	24.72416731636358	25.477021411672247	25.425045658630808	24.37376561333337
40-41	24.92814947486959	25.265952225339312	25.440473655207104	24.365424644584
42-43	24.64309945199931	25.289294365730818	25.33254729878748	24.735058883482395
44-45	24.548696123107533	25.284010781010497	25.526998032853065	24.6402950630289
46-47	24.7311865102264	25.22361947995498	25.293025102876825	24.752168906941794
48-49	24.432422169606202	25.316318227815813	25.493217605461066	24.758041997116916
50-51	24.535478015362084	25.348835497795836	25.577264680318546	24.53842180652353
52-53	24.70270751743034	25.201633465770414	25.494641307601757	24.60101770919749
54-55	24.198744329958846	25.034771245756854	25.83182132235874	24.934663101925562
56-57	24.297554823130188	25.135284486072162	25.740033588493937	24.827127102303713
58-59	24.475780896401186	25.070208083314444	25.642147653063386	24.811863367220983
60-61	24.518291500361066	25.063053288948783	25.796182506240733	24.622472704449414
62-63	24.49104750083137	25.020419983255465	25.81697206269919	24.67156045321398
64-65	24.247547499915886	24.85245646541987	25.80286032885851	25.097135705805734
66-67	24.26537812856673	25.061652498745474	25.660837134217328	25.012132238470468
68-69	24.332839707909923	24.976570314588585	25.70156888778082	24.989021089720676
70-71	24.17636993347404	24.816069192524605	25.810940004691567	25.196620869309783
72-73	24.16111344080918	24.988630205623558	25.69900955122806	25.151246802339205
74-75	24.134154433620132	24.96438150583849	25.787098880784757	25.11436517975662
76-77	24.04161974650648	24.63268610795807	25.92949976225784	25.39619438327761
78-79	24.001071912307477	24.728202784899075	26.016044736716797	25.254680566076647
80-81	24.186793120362115	24.81965250272104	25.765230892419467	25.228323484497377
82-83	24.068020425056297	24.854901878305967	25.879564878397606	25.197512818240135
84-85	24.034143605277016	24.829463738755724	25.932417617578764	25.2039750383885
86-87	23.91165069929198	24.937787790630413	25.88649956742193	25.264061942655687
88-89	23.868841690946812	24.616469353080166	26.049104362733882	25.46558459323914
90-91	23.828309527501414	24.593975640040917	26.10727769704269	25.470437135414976
92-93	24.00229452389756	24.454212964931436	26.138837025550615	25.40465548562039
94-95	23.842951831259537	24.352157147332385	26.265068930001732	25.539822091406343
96-97	23.738941290985853	24.32137639925219	26.316279002674914	25.623403307087038
98-99	23.625590515807538	24.18460663781687	26.29452213482076	25.895280711554836
100-101	23.78006882146583	24.099954456603875	26.4053879144866	25.714588807443693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	48.0
2	71.0
3	104.5
4	99.5
5	106.5
6	168.0
7	313.5
8	487.5
9	514.5
10	438.0
11	436.0
12	499.5
13	551.0
14	632.5
15	752.5
16	851.5
17	936.5
18	1062.0
19	1181.0
20	1244.0
21	1358.0
22	1472.5
23	1538.5
24	1608.5
25	1699.5
26	1962.5
27	2314.5
28	2744.0
29	3364.5
30	4371.5
31	6263.5
32	9833.0
33	14651.5
34	20808.0
35	29182.5
36	40450.5
37	52106.0
38	63046.5
39	73704.5
40	83596.0
41	92873.0
42	102758.5
43	114242.0
44	124587.0
45	135119.5
46	143207.0
47	149351.0
48	155919.0
49	159952.0
50	160464.0
51	158806.0
52	156794.5
53	154722.5
54	151937.0
55	144980.0
56	133517.0
57	119928.5
58	107833.0
59	94067.0
60	76628.5
61	62349.5
62	51546.5
63	39705.5
64	28623.5
65	21149.5
66	16213.5
67	12850.0
68	10074.5
69	7637.5
70	5756.0
71	4162.0
72	2961.5
73	2270.0
74	1741.0
75	1322.0
76	975.5
77	678.5
78	469.0
79	313.5
80	217.5
81	168.5
82	133.5
83	100.0
84	82.5
85	79.0
86	68.5
87	59.0
88	61.0
89	48.0
90	36.0
91	34.5
92	30.5
93	22.5
94	16.5
95	17.5
96	18.0
97	13.0
98	6.5
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05682916631073504
2	0.07622181958238355
3	0.07370407170785731
4	0.07253511733754156
5	0.06791924623424346
6	0.010550562521824227
7	0.014057425632771486
8	0.01876321630301695
9	0.007283484922736613
10-11	0.013487935042104836
12-13	0.02306436892199927
14-15	0.04035889896487592
16-17	0.027905038942665782
18-19	0.010056004903613717
20-21	0.02059158083094672
22-23	0.038125896385683006
24-25	0.03524847024336731
26-27	0.021865441362701063
28-29	0.011629597325192614
30-31	0.012004262187473306
32-33	0.008392492915087455
34-35	0.00511042872150861
36-37	8.692224804912007E-4
38-39	0.004316139213473548
40-41	0.0014536996656490768
42-43	0.24628969386883431
44-45	0.21364889106694063
46-47	0.21982336799732638
48-49	0.2273915982153963
50-51	0.2180399632528703
52-53	0.2267321880577823
54-55	0.22225119630490528
56-57	0.22469401120697535
58-59	0.234285431681361
60-61	0.23917106148550124
62-63	0.22383977532097538
64-65	0.22380980213199292
66-67	0.22103728215111582
68-69	0.21595682661858967
70-71	0.20795398516027414
72-73	0.21937377016258958
74-75	0.21838465492616854
76-77	0.21215023161781787
78-79	0.23032897073567696
80-81	0.2424081658956064
82-83	0.24128417130876428
84-85	0.23510969437837856
86-87	0.257529639737255
88-89	0.2494218921175009
90-91	0.25654052450083403
92-93	0.2515200153462728
94-95	0.2542475755436762
96-97	0.26178583257276367
98-99	0.25489199910679894
100-101	0.26135122133251804
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3336315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.15492619803471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.38017277314198	47.751084347555995
2	11.443413321022627	13.767553675594469
3	3.685469716949821	6.650974764846252
4	1.685862980125987	4.056518525979104
5	0.9519041973495709	2.863086336958145
6	0.6152361318538772	2.2205690463620598
7	0.41108100948467263	1.7309983450874862
8	0.2903778099089927	1.3974124579697926
9	0.22660879553085955	1.2268471833885957
>10	1.2267074445527444	12.997948819655738
>50	0.061653721415986074	2.4911137486379014
>100	0.019964147747356165	2.043796019617453
>500	0.0012483475138462688	0.5238875429097494
>1k	2.9960340174512237E-4	0.27820918543722367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9973188982455193E-5
2	0.0	0.0	0.0	0.0	2.9973188982455193E-5
3	0.0	0.0	0.0	0.0	2.9973188982455193E-5
4	0.0	0.0	0.0	0.0	2.9973188982455193E-5
5	0.0	0.0	0.0	0.0	2.9973188982455193E-5
6	0.0	0.0	0.0	0.0	2.9973188982455193E-5
7	0.0	0.0	0.0	0.0	2.9973188982455193E-5
8	0.0	0.0	0.0	0.0	2.9973188982455193E-5
9	0.0	0.0	0.0	1.1989275592982077E-4	2.9973188982455193E-5
10-11	0.0	0.0	0.0	1.1989275592982077E-4	2.9973188982455193E-5
12-13	0.0	0.0	0.0	1.4986594491227598E-4	2.9973188982455193E-5
14-15	0.0	0.0	0.0	1.4986594491227598E-4	7.493297245613799E-5
16-17	0.0	0.0	0.0	1.6485253940350358E-4	1.3487935042104837E-4
18-19	0.0	0.0	0.0	1.7983913389473116E-4	1.4986594491227598E-4
20-21	0.0	0.0	0.0	2.5477210635086914E-4	2.0981232287718636E-4
22-23	0.0	0.0	0.0	4.1962464575437267E-4	2.5477210635086914E-4
24-25	0.0	0.0	0.0	7.343431300701523E-4	2.6975870084209675E-4
26-27	0.0	0.0	0.0	0.001153967775824525	2.9973188982455195E-4
28-29	0.0	0.0	0.0	0.003386970355017437	2.9973188982455195E-4
30-31	0.0	0.0	0.0	0.012573752778139954	3.147184843157795E-4
32-33	0.0	0.0	0.0	0.03767629855094618	3.596782677894623E-4
34-35	0.0	0.0	0.0	0.07509782499554149	3.896514567719175E-4
36-37	0.0	0.0	0.0	0.13535892144476766	4.196246457543727E-4
38-39	0.0	0.0	0.0	0.23990540461557136	4.196246457543727E-4
40-41	0.0	0.0	0.0	0.3609521283212167	4.196246457543727E-4
42-43	0.0	0.0	0.0	0.48962702862289686	4.196246457543727E-4
44-45	0.0	0.0	0.0	0.6430298098351025	4.346112402456003E-4
46-47	0.0	0.0	0.0	0.8202462896938688	4.795710237192831E-4
48-49	0.0	0.0	0.0	1.01180494048074	5.095442127017383E-4
50-51	0.0	0.0	0.0	1.2113364595369442	5.54503996175421E-4
52-53	0.0	0.0	0.0	1.4166827772557449	6.29436968631559E-4
54-55	0.0	0.0	0.0	1.6528864930319829	7.193565355789246E-4
56-57	0.0	0.0	0.0	1.9229599123583954	7.343431300701523E-4
58-59	0.0	0.0	0.0	2.2061466018646323	7.493297245613798E-4
60-61	0.0	0.0	0.0	2.5069425398980614	7.493297245613798E-4
62-63	0.0	0.0	0.0	2.8202522843316653	7.79302913543835E-4
64-65	0.0	0.0	0.0	3.156896156388111	7.79302913543835E-4
66-67	0.0	0.0	0.0	3.545963135974871	7.79302913543835E-4
68-69	0.0	0.0	0.0	3.962260757752191	7.79302913543835E-4
70-71	0.0	0.0	0.0	4.4047999064836505	7.942895080350627E-4
72-73	0.0	0.0	0.0	4.858459108327601	9.141822639648834E-4
74-75	0.0	0.0	0.0	5.334493295746954	9.29168858456111E-4
76-77	0.0	2.9973188982455193E-5	0.0	5.862860671129674	9.441554529473386E-4
78-79	0.0	2.9973188982455193E-5	0.0	6.442766944967726	9.741286419297938E-4
80-81	0.0	2.9973188982455193E-5	0.0	7.042020912293953	9.891152364210215E-4
82-83	0.0	5.9946377964910385E-5	0.0	7.669060025806916	9.891152364210215E-4
84-85	0.0	5.9946377964910385E-5	0.0	8.322535492002405	9.891152364210215E-4
86-87	0.0	5.9946377964910385E-5	0.0	9.015470661493294	0.001004101830912249
88-89	0.0	8.991956694736558E-5	0.0	9.772968080052394	0.0010190884254034766
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2170	0.0	48.117172	1
GTATCAA	4030	0.0	46.047794	1
TCAACGC	4885	0.0	36.242153	4
TATCAAC	5210	0.0	34.255703	2
ATCAACG	5155	0.0	34.25236	3
CAACGCA	5165	0.0	34.18143	5
AACGCAG	5535	0.0	32.221584	6
GTACATG	9995	0.0	28.0635	1
ACGCAGA	6585	0.0	26.871681	7
TACATGG	10575	0.0	26.302681	2
CGCAGAG	7055	0.0	24.88127	8
ACATGGG	10870	0.0	24.49681	3
CATGGGG	9805	0.0	21.009314	4
ATGGGGG	5835	0.0	19.764374	5
GCAGAGT	8310	0.0	19.696356	9
GAGTACA	7555	0.0	19.09749	1
AGTACAT	7365	0.0	18.689945	2
GCGTAAA	185	1.5320309E-4	17.958227	1
GTATATA	805	0.0	17.68734	1
GTATAGC	1105	0.0	17.609976	1
>>END_MODULE