FastQCFastQC Report
Mon 15 Aug 2016
SRR2921700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336315
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24600.044.8282851
GTATCAA44550.044.0657961
TCAACGC55950.034.5199624
TATCAAC57050.033.7716982
ATCAACG57850.033.1416053
CAACGCA58550.033.0973745
AACGCAG62300.031.2529436
GTACATG100750.026.562111
ACGCAGA76300.025.0197877
TACATGG106700.024.5503222
CGCAGAG80950.022.9959478
ACATGGG110000.022.6928273
CATGGGG93850.020.7818184
GCAGAGT92000.019.201599
TAGCCCG3105.5970304E-918.3856875
AGAGTAC75850.017.9057510-11
ATGGGGG57100.017.8007355
ATATGTA12850.017.385771
GTATTAA14000.017.3157521
TAAGGCG8000.017.2174285
Produced by FastQC (version 0.11.5)