##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921700_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3336315
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.528411136238635	33.0	31.0	34.0	30.0	34.0
2	31.671822654635427	34.0	31.0	34.0	30.0	34.0
3	31.743047943614435	34.0	31.0	34.0	30.0	34.0
4	35.22673398644912	37.0	35.0	37.0	33.0	37.0
5	34.99399786890626	37.0	35.0	37.0	32.0	37.0
6	34.96813580252464	37.0	35.0	37.0	32.0	37.0
7	34.86729190738884	37.0	35.0	37.0	32.0	37.0
8	34.81001314324337	37.0	35.0	37.0	32.0	37.0
9	36.4195353256512	39.0	37.0	39.0	32.0	39.0
10-11	36.32172621589987	39.0	37.0	39.0	32.0	39.0
12-13	36.247423130010205	39.0	37.0	39.0	32.0	39.0
14-15	37.50278450925647	40.0	38.0	41.0	32.0	41.0
16-17	37.46826918321561	40.0	38.0	41.0	32.0	41.0
18-19	37.42497111333911	40.0	38.0	41.0	32.0	41.0
20-21	37.36805412558466	40.0	38.0	41.0	32.0	41.0
22-23	37.28329159566768	40.0	38.0	41.0	31.0	41.0
24-25	37.198930706483054	40.0	37.5	41.0	31.0	41.0
26-27	37.07163097609188	40.0	37.0	41.0	31.0	41.0
28-29	36.95368377985892	40.0	37.0	41.0	30.5	41.0
30-31	36.80287412909153	40.0	37.0	41.0	30.0	41.0
32-33	36.761635367164075	40.0	37.0	41.0	30.0	41.0
34-35	36.89464424072667	40.0	37.0	41.0	30.0	41.0
36-37	36.89528851442385	40.0	37.0	41.0	30.0	41.0
38-39	36.79256904698747	40.0	37.0	41.0	30.0	41.0
40-41	36.71587140302999	40.0	37.0	41.0	30.0	41.0
42-43	36.59917498797326	40.0	37.0	41.0	30.0	41.0
44-45	36.47624549840168	40.0	36.5	41.0	30.0	41.0
46-47	36.326899138720414	40.0	36.0	41.0	29.5	41.0
48-49	36.18592309179439	40.0	36.0	41.0	29.0	41.0
50-51	36.027864425271595	40.0	35.0	41.0	28.5	41.0
52-53	35.85193499414774	39.0	35.0	41.0	28.0	41.0
54-55	35.63342385236406	39.0	35.0	41.0	28.0	41.0
56-57	35.398220192038224	39.0	35.0	41.0	28.0	41.0
58-59	35.15031209583028	38.5	35.0	40.5	27.0	41.0
60-61	34.86510476378879	38.0	35.0	40.0	26.5	41.0
62-63	34.55104089392039	37.5	34.0	40.0	26.0	41.0
64-65	34.20730041977451	37.0	34.0	40.0	26.0	41.0
66-67	33.84863824309156	36.0	34.0	39.0	26.0	41.0
68-69	33.47638382466883	36.0	34.0	39.0	26.0	40.5
70-71	33.0688631019553	35.0	33.0	38.5	25.0	40.0
72-73	32.69772428562651	35.0	33.0	37.0	24.0	39.5
74-75	32.28990847686744	35.0	33.0	37.0	23.5	39.0
76-77	29.580348078643652	33.0	29.0	34.5	19.0	36.5
78-79	31.155972382703673	34.5	31.5	35.5	20.0	37.0
80-81	31.34172118040413	35.0	32.0	35.0	21.0	37.0
82-83	31.174178847021338	35.0	32.0	35.0	20.0	37.0
84-85	30.985335617290332	35.0	32.0	35.0	20.0	36.0
86-87	30.78763755820419	35.0	32.0	35.0	19.5	36.0
88-89	30.530268125162042	35.0	32.0	35.0	17.5	35.5
90-91	30.3384022491881	34.5	32.0	35.0	13.5	35.0
92-93	30.18495016208002	34.0	31.5	35.0	10.0	35.0
94-95	30.087816198410522	34.0	31.5	35.0	4.5	35.0
96-97	29.882986768335723	34.0	31.0	35.0	2.0	35.0
98-99	29.681024423653042	34.0	31.0	35.0	2.0	35.0
100-101	28.564370720390613	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50490.0
3	17429.0
4	9947.0
5	7318.0
6	6400.0
7	6356.0
8	6768.0
9	7570.0
10	8286.0
11	8949.0
12	9611.0
13	10177.0
14	10872.0
15	11684.0
16	12598.0
17	13060.0
18	13035.0
19	11928.0
20	11831.0
21	11814.0
22	12470.0
23	14319.0
24	16955.0
25	20230.0
26	24527.0
27	29601.0
28	36263.0
29	43698.0
30	53385.0
31	66880.0
32	85169.0
33	112925.0
34	162291.0
35	241659.0
36	420563.0
37	818994.0
38	843389.0
39	86873.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47662408712002	19.016308580906642	13.341064150367796	26.16600318160554
2	15.584603598224628	21.08561789185184	36.73622251156367	26.593555998359864
3	18.235497957999293	24.653711213377683	28.595773789967698	28.515017038655326
4	13.613489962860935	16.82078828335996	34.996867609281466	34.568854144497635
5	14.805230937953743	37.03473850556879	33.662702899117754	14.497327657359712
6	33.95470336277774	34.80641322690582	17.11635588673443	14.122527523582008
7	29.4649336168797	31.36181685482336	20.745972727395344	18.427276800901595
8	27.259026800526932	33.87791620395556	19.98252563082323	18.88053136469428
9	27.230118996628917	15.477324819343988	18.81532098457189	38.47723519945521
10-11	25.970590312457496	25.053248810982726	27.52133963437391	21.454821242185865
12-13	26.656696541573517	22.300104561924204	27.207621136045752	23.83557776045653
14-15	23.89935900385341	23.760111062635193	24.447583139234002	27.89294679427739
16-17	24.300047252739514	26.720382625798617	25.166722142052254	23.812847979409614
18-19	24.25784255570546	26.301557465815694	26.250287373289737	23.190312605189114
20-21	24.86135491448403	25.40343640489865	26.06956225394759	23.66564642666973
22-23	24.634409520683747	25.190202363985414	25.82702772370115	24.348360391629686
24-25	24.508533578156104	25.38212344187003	25.750946185449653	24.358396794524218
26-27	24.271517710029052	25.61488056682164	25.782045952046882	24.33155577110242
28-29	24.41746960947222	25.876412777922575	25.508574573377075	24.19754303922813
30-31	24.811274302232423	25.554335598456408	25.371227116123844	24.263162983187325
32-33	24.467695565494914	25.562648601357985	25.34245292082653	24.62720291232057
34-35	24.724613100403694	25.67402980206796	25.46133132370667	24.140025773821673
36-37	24.778532804182056	25.603807970840727	25.45678264831423	24.16087657666299
38-39	24.748944493833612	25.601142458413683	25.371457842397326	24.27845520535537
40-41	24.893793678127327	25.387050217388374	25.409575879107788	24.309580225376514
42-43	24.658187149008864	25.39985392683662	25.30111290484306	24.640846019311454
44-45	24.510973843766422	25.369858956754193	25.514640134841336	24.604527064638052
46-47	24.63636315949994	25.35042567237952	25.347817909188393	24.66539325893215
48-49	24.33940585345616	25.4282658455665	25.507806926353982	24.724521374623354
50-51	24.469036144343576	25.422435357615004	25.548086524856494	24.560441973184926
52-53	24.71421756647575	25.29078058188638	25.435989156041295	24.55901269559658
54-55	24.169796503175796	25.127421564248824	25.75465279047531	24.948129142100072
56-57	24.271885694724507	25.202597443862484	25.617609333675855	24.90790752773715
58-59	24.40011355603667	25.198018585058207	25.560360669321103	24.84150718958402
60-61	24.459332266206243	25.176645503977745	25.68084806628178	24.68317416353423
62-63	24.371274350925546	25.177356897675708	25.7061410523788	24.745227699019946
64-65	24.096072862305498	24.964137371857074	25.693054284504584	25.246735481332845
66-67	24.167665137594042	25.244589137336114	25.515503416795006	25.072242308274838
68-69	24.244883307610614	25.1516336064845	25.464849537448337	25.13863354845655
70-71	24.147139785946045	24.972866452857666	25.53350395061747	25.34648981057882
72-73	24.12230933803228	25.234366415003766	25.354748441292625	25.288575805671325
74-75	24.063496869978174	25.22911519919411	25.386905475739336	25.320482455088385
76-77	24.019073735261635	24.906871925744507	25.51112091244106	25.562933426552792
78-79	23.928854931236355	25.053085499003693	25.58205047127443	25.436009098485524
80-81	24.11038136603599	25.206978558452043	25.265286179744038	25.41735389576793
82-83	23.97605397225385	25.28818356322709	25.331101728644168	25.404660735874895
84-85	23.928917262166628	25.237082906369686	25.3392842844689	25.49471554699479
86-87	23.779367313470555	25.411331728133906	25.22947578066007	25.579825177735472
88-89	23.71263429138237	25.14553666273298	25.32485989714693	25.816969148737723
90-91	23.627461738854624	25.150842338976986	25.373576998065335	25.84811892410306
92-93	23.747729715593646	25.076760804994265	25.365179895297	25.810329584115088
94-95	23.521713174968145	25.055339785307563	25.420209914233638	26.002737125490654
96-97	23.37538143218549	25.091919948030196	25.44351655968029	26.089182060104026
98-99	23.23137967447045	24.930618978523132	25.433903018535275	26.404098328471143
100-101	23.20549445509993	25.057915798566622	25.40241326567773	26.334176480655717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	41.5
2	74.5
3	110.0
4	98.0
5	91.5
6	145.5
7	280.0
8	421.5
9	443.0
10	357.5
11	278.0
12	285.0
13	310.5
14	340.0
15	395.0
16	432.0
17	477.5
18	510.5
19	563.0
20	639.5
21	733.0
22	842.5
23	977.0
24	1162.0
25	1408.0
26	1747.5
27	2183.0
28	2709.5
29	3430.0
30	4675.0
31	6817.5
32	10687.0
33	15703.0
34	21861.5
35	30221.5
36	41249.0
37	52480.0
38	63048.0
39	73285.0
40	82669.5
41	92680.5
42	103413.0
43	114444.0
44	124752.0
45	135959.0
46	144155.5
47	150317.0
48	157123.5
49	160866.5
50	161111.0
51	159855.5
52	157968.5
53	155888.0
54	153851.0
55	146876.5
56	134546.5
57	120107.5
58	107914.0
59	94050.5
60	76142.0
61	61543.5
62	50474.5
63	38555.5
64	27520.5
65	20235.5
66	15408.5
67	12186.0
68	9528.5
69	7084.5
70	5119.5
71	3640.5
72	2583.5
73	1897.5
74	1410.0
75	1054.0
76	739.5
77	431.0
78	258.5
79	152.5
80	86.0
81	53.5
82	47.0
83	28.0
84	13.5
85	9.0
86	6.5
87	5.5
88	4.5
89	3.5
90	3.5
91	2.0
92	1.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1774712519651172
2	8.991956694736558E-5
3	0.011120053112490877
4	0.006144503741403315
5	0.02553715701305183
6	0.0025477210635086915
7	0.0
8	0.0
9	5.9946377964910385E-5
10-11	0.051958523101086077
12-13	4.346112402456003E-4
14-15	0.005380187422350707
16-17	1.4986594491227596E-5
18-19	0.001858337716912222
20-21	2.5477210635086914E-4
22-23	0.0
24-25	7.193565355789246E-4
26-27	0.002937372520280609
28-29	0.006039597579964721
30-31	0.017609248527192426
32-33	0.012543779589157497
34-35	0.011150026301473333
36-37	0.004645844292280555
38-39	2.9973188982455193E-5
40-41	0.0035967826778946234
42-43	0.009456541123964613
44-45	0.007373404489683978
46-47	0.003671715650350761
48-49	0.009081876261683924
50-51	0.0028324663588420156
52-53	0.002382868524105188
54-55	0.0043461124024560035
56-57	0.007972868269333081
58-59	0.015496138703929336
60-61	0.007643163190526075
62-63	0.02213520006354316
64-65	0.04097334933901625
66-67	0.06144503741403315
68-69	0.05138903251041942
70-71	0.07021219519140129
72-73	0.03295552128620949
74-75	0.026226540359648297
76-77	0.23884135640669424
78-79	0.0903092184041375
80-81	0.06815903174610312
82-83	0.026900937111753537
84-85	0.051613831427787846
86-87	0.07066179302613812
88-89	0.2973190481114643
90-91	0.3045276000617448
92-93	0.057878227925120984
94-95	0.009996058525648808
96-97	0.015945736538666162
98-99	8.392492915087454E-4
100-101	1.7983913389473116E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3336315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.163156107834425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6211678610646	46.5147642754442
2	11.803056087394683	13.96612099696114
3	3.8174533260257486	6.775577611860993
4	1.7639125254065462	4.174345284047678
5	1.017894130307064	3.0110914666302597
6	0.6347459312581089	2.2532143571901737
7	0.4320914604566306	1.789472616950046
8	0.3099149387859972	1.466843672283672
9	0.2375182286774229	1.2647095237528816
>10	1.2742642854044555	13.31098154409484
>50	0.0664212373568929	2.6599086642852643
>100	0.01983339855288541	1.9763275712930573
>500	0.0014218970794473824	0.5554582180110414
>1k	3.046922295072591E-4	0.28118419719467513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9973188982455193E-5	0.0
5	0.0	0.0	0.0	2.9973188982455193E-5	0.0
6	0.0	0.0	0.0	2.9973188982455193E-5	0.0
7	0.0	0.0	0.0	2.9973188982455193E-5	2.9973188982455193E-5
8	0.0	0.0	0.0	2.9973188982455193E-5	2.9973188982455193E-5
9	0.0	0.0	0.0	5.9946377964910385E-5	2.9973188982455193E-5
10-11	0.0	0.0	0.0	7.493297245613799E-5	2.9973188982455193E-5
12-13	0.0	0.0	0.0	1.1989275592982077E-4	2.9973188982455193E-5
14-15	0.0	0.0	0.0	1.1989275592982077E-4	5.9946377964910385E-5
16-17	0.0	0.0	0.0	1.1989275592982077E-4	8.991956694736558E-5
18-19	0.0	0.0	0.0	1.1989275592982077E-4	1.0490616143859317E-4
20-21	0.0	0.0	0.0	1.7983913389473118E-4	1.4986594491227598E-4
22-23	0.0	0.0	0.0	3.147184843157795E-4	2.3978551185964154E-4
24-25	0.0	0.0	0.0	6.144503741403315E-4	2.9973188982455195E-4
26-27	0.0	0.0	0.0	0.001004101830912249	2.9973188982455195E-4
28-29	0.0	2.9973188982455193E-5	0.0	0.0032520910045963883	2.9973188982455195E-4
30-31	0.0	2.9973188982455193E-5	0.0	0.012693645534069774	2.9973188982455195E-4
32-33	0.0	2.9973188982455193E-5	0.0	0.03835069530305142	2.9973188982455195E-4
34-35	0.0	2.9973188982455193E-5	0.0	0.07674635038957653	3.297050788070071E-4
36-37	0.0	2.9973188982455193E-5	0.0	0.13805650845318862	3.446916732982347E-4
38-39	0.0	2.9973188982455193E-5	0.0	0.2433972811320274	3.596782677894623E-4
40-41	0.0	2.9973188982455193E-5	0.0	0.3648036831054622	3.596782677894623E-4
42-43	0.0	5.9946377964910385E-5	0.0	0.4940630605923002	3.596782677894623E-4
44-45	0.0	5.9946377964910385E-5	0.0	0.6483050910960146	4.1962464575437267E-4
46-47	0.0	5.9946377964910385E-5	0.0	0.8255065843602897	5.095442127017383E-4
48-49	0.0	5.9946377964910385E-5	0.0	1.0173799536314767	5.395174016841935E-4
50-51	0.0	5.9946377964910385E-5	0.0	1.2179455477075756	5.54503996175421E-4
52-53	0.0	5.9946377964910385E-5	0.0	1.4244158600132182	6.29436968631559E-4
54-55	0.0	8.991956694736558E-5	0.0	1.6617885301597721	7.04369941087697E-4
56-57	0.0	8.991956694736558E-5	0.0	1.9324464266713424	7.493297245613798E-4
58-59	0.0	8.991956694736558E-5	0.0	2.216022767634351	7.79302913543835E-4
60-61	0.0	8.991956694736558E-5	0.0	2.5177478745262363	8.092761025262902E-4
62-63	0.0	8.991956694736558E-5	0.0	2.8321666269521915	8.692224804912007E-4
64-65	0.0	8.991956694736558E-5	0.0	3.171118434560286	8.692224804912007E-4
66-67	0.0	8.991956694736558E-5	0.0	3.562358470348274	8.991956694736559E-4
68-69	0.0	8.991956694736558E-5	0.0	3.9811588534056286	8.991956694736559E-4
70-71	0.0	8.991956694736558E-5	0.0	4.4265754282794045	9.29168858456111E-4
72-73	0.0	8.991956694736558E-5	0.0	4.883756479828794	9.741286419297938E-4
74-75	0.0	8.991956694736558E-5	0.0	5.36313267781969	9.891152364210215E-4
76-77	0.0	8.991956694736558E-5	0.0	5.894047774265919	9.891152364210215E-4
78-79	0.0	8.991956694736558E-5	0.0	6.479004530447515	9.891152364210215E-4
80-81	0.0	8.991956694736558E-5	0.0	7.083039221416443	0.0010340750198947041
82-83	0.0	8.991956694736558E-5	0.0	7.7145293534933	0.001109007992350842
84-85	0.0	8.991956694736558E-5	0.0	8.373399993705629	0.001109007992350842
86-87	0.0	8.991956694736558E-5	0.0	9.073139077095538	0.0011389811813332972
88-89	0.0	8.991956694736558E-5	0.0	9.837470382742637	0.0011689543703157525
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2460	0.0	44.828285	1
GTATCAA	4455	0.0	44.065796	1
TCAACGC	5595	0.0	34.519962	4
TATCAAC	5705	0.0	33.771698	2
ATCAACG	5785	0.0	33.141605	3
CAACGCA	5855	0.0	33.097374	5
AACGCAG	6230	0.0	31.252943	6
GTACATG	10075	0.0	26.56211	1
ACGCAGA	7630	0.0	25.019787	7
TACATGG	10670	0.0	24.550322	2
CGCAGAG	8095	0.0	22.995947	8
ACATGGG	11000	0.0	22.692827	3
CATGGGG	9385	0.0	20.781818	4
GCAGAGT	9200	0.0	19.20159	9
TAGCCCG	310	5.5970304E-9	18.385687	5
AGAGTAC	7585	0.0	17.90575	10-11
ATGGGGG	5710	0.0	17.800735	5
ATATGTA	1285	0.0	17.38577	1
GTATTAA	1400	0.0	17.315752	1
TAAGGCG	800	0.0	17.217428	5
>>END_MODULE