FastQCFastQC Report
Mon 15 Aug 2016
SRR2921699_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921699_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499879
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.047.8558541
GTATCAA34750.043.9806481
TCAACGC40100.037.0476384
CAACGCA41250.036.0119135
ATCAACG42600.034.6506463
TATCAAC43750.033.6340372
AACGCAG45950.032.520036
ACGCAGA54200.027.1378357
GTACATG75700.025.832231
CGCAGAG57600.025.3727268
TACATGG78600.024.2168242
ACATGGG80450.023.127073
CATGGGG66150.020.9514264
GCAGAGT66550.020.607419
AGAGTAC55400.018.41648310-11
GTATATA8000.018.3921411
GAGTACA58650.017.2374171
CAGAGTA59200.017.19426310-11
AGTACAT55750.016.773232
TATACTG11300.016.3799295
Produced by FastQC (version 0.11.5)