##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921699_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2499879
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.4668405950848	31.0	31.0	34.0	26.0	34.0
2	30.522755701375946	31.0	31.0	34.0	26.0	34.0
3	30.587641641855466	33.0	31.0	34.0	26.0	34.0
4	34.0387434751842	37.0	35.0	37.0	30.0	37.0
5	34.03885108039229	37.0	35.0	37.0	30.0	37.0
6	34.08167715317421	37.0	35.0	37.0	30.0	37.0
7	34.00395579146031	37.0	35.0	37.0	30.0	37.0
8	33.971536622372525	37.0	35.0	37.0	30.0	37.0
9	35.53909849236703	39.0	37.0	39.0	30.0	39.0
10-11	35.437101155695935	39.0	37.0	39.0	30.0	39.0
12-13	35.33570164795976	39.0	35.0	39.0	29.0	39.0
14-15	36.484548052125724	40.0	37.0	41.0	29.0	41.0
16-17	36.427231077984175	40.0	37.0	41.0	29.0	41.0
18-19	36.392766609903916	40.0	37.0	41.0	28.5	41.0
20-21	36.31833000717235	40.0	37.0	41.0	28.0	41.0
22-23	36.22468187460273	40.0	37.0	41.0	27.5	41.0
24-25	36.11706546596855	40.0	36.0	41.0	27.0	41.0
26-27	35.97651966355171	40.0	36.0	41.0	27.0	41.0
28-29	35.84173193982589	40.0	36.0	41.0	27.0	41.0
30-31	35.69740115421587	40.0	36.0	41.0	26.5	41.0
32-33	35.529611833212726	39.5	35.5	41.0	25.5	41.0
34-35	35.366469737135276	39.0	35.0	41.0	25.0	41.0
36-37	35.34055668294346	39.0	35.0	41.0	25.0	41.0
38-39	35.52769654051256	40.0	35.5	41.0	25.5	41.0
40-41	35.59797294189039	40.0	36.0	41.0	25.5	41.0
42-43	35.44790327851868	40.0	35.0	41.0	25.0	41.0
44-45	35.33921821816176	40.0	35.0	41.0	24.5	41.0
46-47	35.18867993210871	40.0	35.0	41.0	24.0	41.0
48-49	35.03100870082112	40.0	35.0	41.0	23.0	41.0
50-51	33.34226356555658	37.0	32.5	39.5	20.0	40.0
52-53	33.46439167655714	37.5	33.5	39.5	21.0	40.0
54-55	34.38615308980955	39.0	34.0	40.5	22.0	41.0
56-57	34.41600973487117	39.0	34.0	41.0	22.0	41.0
58-59	34.232013429449985	38.5	34.0	41.0	21.5	41.0
60-61	33.98246595135204	38.0	34.0	40.5	20.0	41.0
62-63	33.69059982503153	37.5	34.0	40.0	19.5	41.0
64-65	33.4063864691051	37.0	34.0	40.0	18.0	41.0
66-67	33.06738046121432	36.5	33.0	39.5	17.0	41.0
68-69	32.70879970590576	36.0	33.0	39.0	13.5	41.0
70-71	32.30515676958765	35.5	33.0	39.0	11.0	40.5
72-73	31.943562268413793	35.0	32.5	37.5	7.5	40.0
74-75	31.54258206097175	35.0	32.0	37.0	6.0	39.0
76-77	31.165920430548837	35.0	32.0	37.0	2.0	39.0
78-79	30.82722783782735	35.0	32.0	36.0	2.0	38.5
80-81	30.51469371117562	35.0	31.5	36.0	2.0	37.0
82-83	30.209166123640387	35.0	31.0	35.0	2.0	37.0
84-85	29.955487645602048	35.0	31.0	35.0	2.0	36.5
86-87	29.733857918723267	35.0	31.0	35.0	2.0	36.0
88-89	29.530815691479468	35.0	31.0	35.0	2.0	36.0
90-91	29.320796926571248	35.0	31.0	35.0	2.0	35.5
92-93	29.14890180684745	34.0	31.0	35.0	2.0	35.0
94-95	28.936549929016564	34.0	30.5	35.0	2.0	35.0
96-97	28.72192834133172	34.0	30.0	35.0	2.0	35.0
98-99	28.47756811429673	34.0	30.0	35.0	2.0	35.0
100-101	27.42372030806291	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80789.0
3	14000.0
4	9663.0
5	7104.0
6	6615.0
7	6905.0
8	7267.0
9	7847.0
10	8585.0
11	9133.0
12	9062.0
13	9127.0
14	9748.0
15	10291.0
16	10058.0
17	10398.0
18	10290.0
19	10032.0
20	9918.0
21	10408.0
22	11679.0
23	13044.0
24	15467.0
25	18275.0
26	21713.0
27	25874.0
28	30498.0
29	36331.0
30	43607.0
31	52942.0
32	66948.0
33	86622.0
34	120031.0
35	175424.0
36	293182.0
37	539494.0
38	592703.0
39	98796.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23128722952499	17.651282498726246	13.108555021150512	25.008875250598255
2	15.966094217690207	19.411316579269418	39.38128432522105	25.241304877819324
3	19.59472956284514	23.523899306869062	28.606392496652433	28.274978633633364
4	13.396336235021533	15.803779373488341	36.64017171546536	34.15971267602477
5	14.802281326204989	36.77725846971175	33.91555346337917	14.504906740704094
6	33.54109774822314	34.80556691281016	17.537023538721392	14.116311800245319
7	29.629869675238297	31.043018173452957	20.6714875826407	18.655624568668046
8	26.836166329395706	34.12706033349417	19.892985484145864	19.143787852964262
9	26.84712687821036	15.964468020418288	19.032491639063576	38.15591346230778
10-11	25.749825517301943	25.082420445591502	27.63923959349982	21.528514443606735
12-13	26.604622317360448	22.35068994663479	27.274773310393464	23.7699144256113
14-15	23.708349974268245	23.863580883747694	24.72793572103737	27.70013342094669
16-17	24.000865880891762	26.825782283772853	25.384534743070652	23.78881709226473
18-19	23.889869483952737	26.43119983229424	26.500110416960045	23.17882026679298
20-21	24.591404582382253	25.608006865771372	26.404572379673958	23.39601617217242
22-23	24.345111171181593	25.449409774196134	26.040285859891675	24.1651931947306
24-25	24.139549605824065	25.57371696243034	26.136139456769097	24.150593974976502
26-27	24.007499492872	25.84210254668278	26.049394867264496	24.10100309318072
28-29	24.12340316663236	25.961060499588832	25.877286945027706	24.0382493887511
30-31	24.463078126040795	25.70735945075913	25.80661551038958	24.022946912810493
32-33	24.12471481246932	25.720104765787177	25.749548640601105	24.405631781142397
34-35	24.446746002409835	25.821700197939318	25.808218882162898	23.923334917487946
36-37	24.503127274501917	25.61528933314479	25.78725892904824	24.094324463305053
38-39	24.304117092185134	25.78111061239936	25.829554991771257	24.08521730364425
40-41	24.54515161746093	25.600997502244137	25.866634077966467	23.987216802328465
42-43	24.326055485710977	25.49961818815696	25.78335581034627	24.3909705157858
44-45	24.262454888912536	25.62630433096502	25.89844892213386	24.212791857988584
46-47	24.398260044499022	25.58307008786563	25.677110902621457	24.341558965013892
48-49	24.20661789495923	25.59606635293382	25.78698798426445	24.410327767842503
50-51	24.243347409813985	25.732782032344502	25.8551812739382	24.16868928390331
52-53	24.484316941183167	25.53801212023057	25.8217756053601	24.155895333226166
54-55	24.07490809661736	25.4133973595403	26.101342357613145	24.410352186229193
56-57	24.12465384621552	25.573007781745527	25.960992657611214	24.341345714427735
58-59	24.245499719061314	25.48295294522568	25.929508143814147	24.342039191898863
60-61	24.31759956058231	25.53481863997017	26.03872568869502	24.1088561107525
62-63	24.18896296937686	25.48833793530304	26.071401083306878	24.251298012013226
64-65	24.039196208737508	25.356753727484165	26.044485279674557	24.559564784103763
66-67	24.029005468940724	25.508421479231615	25.930708044028922	24.531865007798743
68-69	24.14064499500654	25.43359320432578	26.00615248699927	24.41960931366841
70-71	23.99737311575966	25.279652549067706	26.044050211405263	24.67892412376737
72-73	23.981364287296227	25.369001341859505	25.967576906387514	24.682057464456758
74-75	23.977818588716925	25.483329368263096	26.001948529713875	24.5369035133061
76-77	23.982256386556493	25.18302059486478	26.097196667000016	24.73752635157871
78-79	23.883005389460536	25.210395823774444	26.16302762321523	24.74357116354979
80-81	24.017914151999655	25.352173664632883	25.993382428855092	24.636529754512367
82-83	24.00895778989328	25.299421604704143	26.11581457671616	24.57580602868642
84-85	24.003912905773543	25.297797787764953	26.072100099827207	24.6261892066343
86-87	23.83921077841478	25.383080192108505	26.115817674158986	24.66189135531773
88-89	23.77851991356867	25.153117297773903	26.196820934056614	24.871541854600817
90-91	23.818531811407787	25.194355875306684	26.226088588385384	24.76102372490015
92-93	23.829182958713982	25.0831869279489	26.326765590795098	24.76086452254202
94-95	23.737332119951567	25.055411167402774	26.331266454184515	24.87599025846114
96-97	23.705207361915953	24.908814172195495	26.39884603886278	24.987132427025774
98-99	23.496134475347255	24.813861354377185	26.429261700830846	25.260742469444715
100-101	23.71272370588641	24.725866552030016	26.558161525394635	25.003248216688934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	16.5
2	24.5
3	44.5
4	46.5
5	49.0
6	82.0
7	176.5
8	285.0
9	317.0
10	285.0
11	289.0
12	340.0
13	410.5
14	464.0
15	550.0
16	647.5
17	723.5
18	792.0
19	831.0
20	901.5
21	979.5
22	1050.0
23	1117.5
24	1236.5
25	1416.5
26	1671.5
27	1988.0
28	2390.0
29	3120.0
30	4418.0
31	6623.5
32	10501.5
33	15736.5
34	22082.5
35	29963.5
36	39509.5
37	49220.0
38	57897.5
39	65058.0
40	70692.5
41	76314.5
42	82568.5
43	89080.5
44	94790.0
45	100546.0
46	105443.0
47	110157.0
48	114667.0
49	116169.5
50	114656.0
51	112649.5
52	110870.5
53	108586.5
54	106225.5
55	101147.0
56	93021.0
57	83671.0
58	75318.5
59	65648.5
60	54127.0
61	44602.5
62	36432.0
63	28119.5
64	20392.0
65	14969.0
66	11267.5
67	8648.5
68	6706.5
69	4987.0
70	3729.5
71	2730.0
72	1917.5
73	1442.0
74	1105.5
75	827.0
76	625.0
77	414.5
78	256.5
79	191.5
80	156.5
81	127.0
82	102.0
83	81.0
84	61.0
85	55.0
86	46.5
87	40.5
88	35.0
89	30.0
90	31.0
91	25.5
92	21.5
93	21.5
94	16.5
95	11.5
96	8.5
97	5.5
98	6.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05432262921525402
2	0.07592367470585576
3	0.07176347335211025
4	0.07172347141601654
5	0.06732325844570877
6	0.010120489831707855
7	0.014160685377172254
8	0.01620078411795131
9	0.006360307838899403
10-11	0.013900672792563162
12-13	0.023661145199427653
14-15	0.04084197675167479
16-17	0.027601335904657787
18-19	0.010520509192644923
20-21	0.020961014513102436
22-23	0.038501863490192925
24-25	0.03472168052933762
26-27	0.02040098740779054
28-29	0.011340548882565917
30-31	0.011340548882565917
32-33	0.008320402707491042
34-35	0.004920238139525953
36-37	7.400358177335783E-4
38-39	0.004200203289839229
40-41	0.0014000677632797426
42-43	0.23399132518013874
44-45	0.2026298072826725
46-47	0.20871010156891592
48-49	0.21601045490601745
50-51	0.20697001734883966
52-53	0.21489040069539367
54-55	0.21103021386235093
56-57	0.212930305826802
58-59	0.22251076952124485
60-61	0.2267709757152246
62-63	0.21181025161617822
64-65	0.21205026323274045
66-67	0.20985015674758659
68-69	0.20462990408735782
70-71	0.19632950234791366
72-73	0.20827008027188518
74-75	0.20711002412516763
76-77	0.20038969886142488
78-79	0.2189505972089049
80-81	0.23047115480389252
82-83	0.22949110736959671
84-85	0.2227707821058539
86-87	0.24321177144973816
88-89	0.23505137648662197
90-91	0.24259174144028572
92-93	0.237911514917322
94-95	0.24091166012435
96-97	0.24831201830168578
98-99	0.2415716920698962
100-101	0.2481320095892641
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2499879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.66195544472727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.99429394027659	53.18243719983585
2	11.152492608769435	14.645889455493377
3	3.401265254559001	6.700011828016561
4	1.4680753192823777	3.8558678481689297
5	0.7870732241366347	2.5840383487498775
6	0.5008301734010364	1.973129311874033
7	0.332764324234678	1.5294969362044546
8	0.24141431248982403	1.2681388664341033
9	0.1783892303518232	1.0542047125662544
>10	0.8968628305094432	10.245789419904064
>50	0.03674322063228294	1.60736085394144
>100	0.009063537456656182	0.9885329768066586
>500	6.100199169039809E-4	0.2673677965318361
>1k	1.2200398337877003E-4	0.09773444547249174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.200058082811208E-4
7	0.0	0.0	0.0	0.0	1.200058082811208E-4
8	0.0	0.0	0.0	0.0	1.6000774437482774E-4
9	0.0	0.0	0.0	4.0001936093706936E-5	1.6000774437482774E-4
10-11	0.0	0.0	0.0	4.0001936093706936E-5	1.6000774437482774E-4
12-13	0.0	0.0	0.0	4.0001936093706936E-5	2.2001064851538812E-4
14-15	0.0	0.0	0.0	4.0001936093706936E-5	2.8001355265594853E-4
16-17	0.0	0.0	0.0	8.000387218741387E-5	3.6001742484336244E-4
18-19	0.0	0.0	0.0	8.000387218741387E-5	3.6001742484336244E-4
20-21	0.0	0.0	0.0	1.0000484023426734E-4	5.000242011713367E-4
22-23	2.0000968046853468E-5	0.0	0.0	1.4000677632797426E-4	6.200300094524574E-4
24-25	4.0001936093706936E-5	0.0	0.0	2.2001064851538812E-4	7.200348496867249E-4
26-27	4.0001936093706936E-5	2.0000968046853468E-5	0.0	4.400212970307763E-4	7.200348496867249E-4
28-29	4.0001936093706936E-5	4.0001936093706936E-5	0.0	0.0015600755076545705	7.200348496867249E-4
30-31	4.0001936093706936E-5	4.0001936093706936E-5	0.0	0.006760327199836472	7.400358177335783E-4
32-33	4.0001936093706936E-5	4.0001936093706936E-5	0.0	0.020200977727322003	8.000387218741387E-4
34-35	4.0001936093706936E-5	4.0001936093706936E-5	0.0	0.03942190802034819	8.800425940615526E-4
36-37	4.0001936093706936E-5	4.0001936093706936E-5	0.0	0.07048341139711162	8.800425940615526E-4
38-39	4.0001936093706936E-5	4.0001936093706936E-5	0.0	0.12480604061236564	9.40045498202113E-4
40-41	4.0001936093706936E-5	4.0001936093706936E-5	0.0	0.18974918386049883	9.8004743429582E-4
42-43	4.0001936093706936E-5	8.000387218741387E-5	0.0	0.2602125942895636	0.0010400503384363803
44-45	4.0001936093706936E-5	8.000387218741387E-5	0.0	0.3489368885454056	0.0011000532425769407
46-47	4.0001936093706936E-5	8.000387218741387E-5	0.0	0.45118183720092053	0.001160056146717501
48-49	4.0001936093706936E-5	8.000387218741387E-5	0.0	0.5577869968906495	0.0013600658271860357
50-51	4.0001936093706936E-5	8.000387218741387E-5	0.0	0.6788128545421599	0.0014000677632797426
52-53	4.0001936093706936E-5	8.000387218741387E-5	0.0	0.8035188903142911	0.0015200735715608636
54-55	6.000290414056041E-5	8.000387218741387E-5	0.0	0.9425856211440634	0.0016800813159356914
56-57	8.000387218741387E-5	8.000387218741387E-5	0.0	1.1050134826525604	0.0017200832520293983
58-59	8.000387218741387E-5	8.000387218741387E-5	0.0	1.2819820479311197	0.0017400842200762517
60-61	8.000387218741387E-5	8.000387218741387E-5	0.0	1.4801116374032501	0.0017600851881231052
62-63	8.000387218741387E-5	8.000387218741387E-5	0.0	1.6793612810860046	0.001840089060310519
64-65	8.000387218741387E-5	8.000387218741387E-5	0.0	1.8932916353151494	0.0018600900283573724
66-67	8.000387218741387E-5	8.000387218741387E-5	0.0	2.145123823993081	0.001880090996404226
68-69	8.000387218741387E-5	8.000387218741387E-5	0.0	2.418157038800678	0.001880090996404226
70-71	8.000387218741387E-5	8.000387218741387E-5	0.0	2.7093511325948176	0.001880090996404226
72-73	8.000387218741387E-5	8.000387218741387E-5	0.0	3.0194461411932334	0.0021001016449196143
74-75	8.000387218741387E-5	8.000387218741387E-5	0.0	3.350822179793502	0.002180105517107028
76-77	8.000387218741387E-5	8.000387218741387E-5	0.0	3.7114396336782702	0.002260109389294442
78-79	1.200058082811208E-4	8.000387218741387E-5	0.0	4.11335908657979	0.0023201122934350025
80-81	1.200058082811208E-4	8.000387218741387E-5	0.0	4.54121979503808	0.0023401132614818557
82-83	1.200058082811208E-4	8.000387218741387E-5	0.0	4.991141571252049	0.002440118101716123
84-85	1.200058082811208E-4	8.000387218741387E-5	0.0	5.4667045885020835	0.002440118101716123
86-87	1.200058082811208E-4	8.000387218741387E-5	0.0	5.974029143010522	0.002560123909997244
88-89	1.200058082811208E-4	8.000387218741387E-5	0.0	6.51847549421392	0.0026401277821846577
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1815	0.0	47.855854	1
GTATCAA	3475	0.0	43.980648	1
TCAACGC	4010	0.0	37.047638	4
CAACGCA	4125	0.0	36.011913	5
ATCAACG	4260	0.0	34.650646	3
TATCAAC	4375	0.0	33.634037	2
AACGCAG	4595	0.0	32.52003	6
ACGCAGA	5420	0.0	27.137835	7
GTACATG	7570	0.0	25.83223	1
CGCAGAG	5760	0.0	25.372726	8
TACATGG	7860	0.0	24.216824	2
ACATGGG	8045	0.0	23.12707	3
CATGGGG	6615	0.0	20.951426	4
GCAGAGT	6655	0.0	20.60741	9
AGAGTAC	5540	0.0	18.416483	10-11
GTATATA	800	0.0	18.392141	1
GAGTACA	5865	0.0	17.237417	1
CAGAGTA	5920	0.0	17.194263	10-11
AGTACAT	5575	0.0	16.77323	2
TATACTG	1130	0.0	16.379929	5
>>END_MODULE