FastQCFastQC Report
Mon 15 Aug 2016
SRR2921699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499879
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.045.34691
GTATCAA31700.045.286721
TCAACGC39200.035.9589164
CAACGCA41050.034.251525
TATCAAC41850.033.6799282
ATCAACG41900.033.526473
AACGCAG44450.032.056496
GTACATG75850.026.5725631
ACGCAGA55850.025.0869567
TACATGG78600.024.7554872
CGCAGAG59700.023.5486768
ACATGGG81650.023.5401363
CATGGGG65700.020.9492684
TCTATAC7100.019.3842853
GCAGAGT70250.019.134429
TATACTG9300.018.8981865
TAGATCG1350.00940243917.5929035
AGAGTAC58250.017.08296810-11
ATGGGGG35050.017.0759095
GTATATG9200.017.0509781
Produced by FastQC (version 0.11.5)