##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921699_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2499879
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43049483595006	33.0	31.0	34.0	29.0	34.0
2	31.56735866015915	34.0	31.0	34.0	29.0	34.0
3	31.64213147916359	34.0	31.0	34.0	28.0	34.0
4	35.11871734591954	37.0	35.0	37.0	33.0	37.0
5	34.87130377110252	37.0	35.0	37.0	32.0	37.0
6	34.851207598447765	37.0	35.0	37.0	32.0	37.0
7	34.745250470122755	37.0	35.0	37.0	32.0	37.0
8	34.689758984334844	37.0	35.0	37.0	32.0	37.0
9	36.288061942198006	39.0	37.0	39.0	32.0	39.0
10-11	36.181453582353384	39.0	37.0	39.0	32.0	39.0
12-13	36.10791402303872	39.0	37.0	39.0	32.0	39.0
14-15	37.36144389388446	40.0	38.0	41.0	32.0	41.0
16-17	37.32877991294779	40.0	38.0	41.0	32.0	41.0
18-19	37.293744817249156	40.0	38.0	41.0	31.0	41.0
20-21	37.23606062533427	40.0	38.0	41.0	31.0	41.0
22-23	37.14671550103025	40.0	38.0	41.0	31.0	41.0
24-25	37.070682421029176	40.0	37.5	41.0	31.0	41.0
26-27	36.947796873368674	40.0	37.0	41.0	30.5	41.0
28-29	36.828321090740786	40.0	37.0	41.0	30.0	41.0
30-31	36.68435592282667	40.0	37.0	41.0	30.0	41.0
32-33	36.63953395344335	40.0	37.0	41.0	30.0	41.0
34-35	36.77949852772875	40.0	37.0	41.0	30.0	41.0
36-37	36.782760485607504	40.0	37.0	41.0	30.0	41.0
38-39	36.6799481094885	40.0	37.0	41.0	30.0	41.0
40-41	36.60691417464605	40.0	37.0	41.0	30.0	41.0
42-43	36.49777109212086	40.0	37.0	41.0	30.0	41.0
44-45	36.372710639194935	40.0	36.5	41.0	29.5	41.0
46-47	36.230194541415806	40.0	36.0	41.0	29.0	41.0
48-49	36.095848239054774	40.0	36.0	41.0	28.5	41.0
50-51	35.942985640505	40.0	35.5	41.0	28.0	41.0
52-53	35.77517911866934	39.5	35.0	41.0	28.0	41.0
54-55	35.56794688862941	39.0	35.0	41.0	28.0	41.0
56-57	35.34377463869251	39.0	35.0	41.0	27.0	41.0
58-59	35.10603753221656	39.0	35.0	40.5	26.5	41.0
60-61	34.83464779695338	38.0	35.0	40.0	26.0	41.0
62-63	34.53510229895127	37.5	34.0	40.0	26.0	41.0
64-65	34.19968666483458	37.0	34.0	40.0	26.0	41.0
66-67	33.84896028967802	36.5	34.0	39.5	25.5	41.0
68-69	33.48831983468	36.0	34.0	39.0	25.0	41.0
70-71	33.08782685081958	35.5	33.5	39.0	24.0	40.0
72-73	32.72356062033403	35.0	33.0	37.5	23.0	40.0
74-75	32.315435267066924	35.0	33.0	37.0	22.5	39.0
76-77	29.606474153349023	33.0	29.0	34.5	18.5	36.5
78-79	31.17578730810571	34.5	31.5	36.0	20.0	37.5
80-81	31.352270249880092	35.0	32.0	36.0	20.0	37.0
82-83	31.17237994318925	35.0	32.0	35.0	20.0	37.0
84-85	30.97235106179139	35.0	32.0	35.0	19.5	36.5
86-87	30.766043476504265	35.0	32.0	35.0	18.0	36.0
88-89	30.49690485019475	35.0	32.0	35.0	14.5	36.0
90-91	30.30352249048854	34.5	32.0	35.0	9.0	35.0
92-93	30.14694311204662	34.0	31.5	35.0	7.0	35.0
94-95	30.045686611231986	34.0	31.5	35.0	2.0	35.0
96-97	29.84993693694775	34.0	31.0	35.0	2.0	35.0
98-99	29.65470708782305	34.0	31.0	35.0	2.0	35.0
100-101	28.53551211878655	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43741.0
3	14833.0
4	7993.0
5	5677.0
6	5019.0
7	5058.0
8	5252.0
9	5755.0
10	6287.0
11	6708.0
12	7349.0
13	7892.0
14	8406.0
15	8796.0
16	9424.0
17	9962.0
18	9610.0
19	8810.0
20	8628.0
21	8488.0
22	8957.0
23	10544.0
24	12146.0
25	15217.0
26	17863.0
27	21763.0
28	26117.0
29	32048.0
30	38972.0
31	48266.0
32	61777.0
33	81869.0
34	116611.0
35	175122.0
36	308942.0
37	604354.0
38	648467.0
39	77153.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81279553533041	19.439287762107607	13.857569250581905	25.890347451980077
2	14.995331772190482	20.870778493990507	37.89720938381793	26.23668035000108
3	18.097412523598617	24.850087033333505	28.509847708668968	28.54265273439891
4	13.061073450961016	17.14345150108313	35.70479598511018	34.090679062845666
5	14.460152055867187	37.19510902031959	34.07952823548853	14.265210688324684
6	33.37397627588237	35.61121825220146	17.288251669420866	13.7265538024953
7	29.206053572992936	31.326876220809087	20.879850584768302	18.587219621429675
8	26.722693378359512	34.73628123601182	19.748035804932957	18.792989580695707
9	26.633859732354935	15.959618829398876	19.09825199469734	38.308269443548845
10-11	25.563401377669177	25.42234467103211	27.721935188355427	21.29231876294329
12-13	26.349187999075152	22.614179754659634	27.47834490858339	23.55828733768182
14-15	23.508692062252575	24.16493716176645	24.709011133904482	27.617359642076494
16-17	23.870255320357504	27.17913547015676	25.385088638290092	23.56552057119565
18-19	23.792757907537712	26.712916478320526	26.502942200069608	22.991383414072157
20-21	24.44255235059659	25.776818530199098	26.462832556494803	23.31779656270951
22-23	24.17783504278308	25.596103970652972	26.130829958335976	24.095231028227975
24-25	24.030396865748244	25.823075104515723	26.154573270615227	23.9919547591208
26-27	23.893990279655423	26.014572716270067	26.14148243755472	23.949954566519796
28-29	23.965580523632887	26.16812298042102	25.991085099595058	23.87521139635103
30-31	24.355849341871398	25.92169910983253	25.78931122894482	23.933140319351256
32-33	24.06666876035048	25.94278282184438	25.758371951464675	24.232176466340466
34-35	24.28952916654998	26.016218530090697	25.83407011493793	23.860182188421394
36-37	24.398125109009943	25.819894774403423	25.856498112627875	23.925482003958763
38-39	24.248938498411025	26.015684362260004	25.78799329647421	23.947383842854762
40-41	24.427002097375343	25.81699830105797	25.81483812046687	23.941161481099822
42-43	24.28961258942352	25.71828860523711	25.72933027473754	24.26276853060183
44-45	24.219118326848864	25.78157175858371	25.832518065736537	24.166791848830886
46-47	24.282842320418805	25.80753557860957	25.643901276971608	24.265720824000017
48-49	24.11233856791609	25.83819466503018	25.776306198687983	24.273160568365753
50-51	24.185634574085395	25.88700081546051	25.764511726770127	24.162852883683968
52-53	24.367319826635622	25.698554876818203	25.728557018971156	24.20556827757502
54-55	23.992112072733107	25.62700280965905	25.997837000571455	24.38304811703639
56-57	24.055427759886385	25.725501570219834	25.87300222031084	24.34606844958294
58-59	24.144564160863606	25.591830112885866	25.884912070411687	24.378693655838845
60-61	24.183198979227505	25.706816744813477	25.970670993094902	24.139313282864116
62-63	24.128927197124813	25.644600976066467	25.916415181344505	24.31005664546421
64-65	23.947975808751067	25.496375814753865	25.9210933083884	24.63455506810667
66-67	23.96992741825573	25.73488840959583	25.75720308819141	24.53798108395703
68-69	24.009018663077452	25.62010312589488	25.77436973116708	24.596508479860592
70-71	23.9169689902652	25.47664252481135	25.81707430815832	24.789314176765135
72-73	23.920367215801708	25.590301161935013	25.725494103490927	24.763837518772352
74-75	23.860839760617147	25.666834655335467	25.696264349476717	24.77606123457067
76-77	23.805737799072986	25.419076561121457	25.89011983868617	24.88506580111938
78-79	23.738939705040117	25.431256667305245	25.925505544135184	24.904298083519453
80-81	23.898885811321858	25.56225308328785	25.70964005675319	24.829221048637102
82-83	23.896547991862093	25.552238674583165	25.77273201066675	24.778481322887995
84-85	23.775245319145018	25.520995508652504	25.774699409505068	24.929059762697403
86-87	23.64676156671952	25.63812553114581	25.76255825841417	24.9525546437205
88-89	23.612516459629074	25.44433977328441	25.799009172456795	25.14413459462973
90-91	23.515542404333218	25.484868849992477	25.868599227644317	25.13098951802999
92-93	23.599566223290942	25.32963007507843	25.925262570389158	25.145541131241465
94-95	23.404122959478308	25.322761929584566	25.978077212426687	25.295037898510444
96-97	23.384165881847434	25.25735002884561	25.94586387593748	25.41262021336948
98-99	23.147102531946853	25.16991985535161	25.94180462466823	25.741172988033306
100-101	23.146191197274582	25.224716370648352	26.001015651391963	25.628076780685106
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	16.5
2	20.5
3	34.0
4	39.0
5	36.0
6	73.0
7	182.5
8	244.0
9	248.5
10	216.0
11	180.5
12	186.0
13	194.0
14	225.5
15	266.5
16	290.0
17	329.5
18	367.0
19	394.0
20	457.5
21	545.0
22	658.0
23	806.0
24	963.0
25	1135.5
26	1461.5
27	1927.0
28	2527.5
29	3444.0
30	5032.5
31	7613.5
32	11954.0
33	17808.5
34	24260.0
35	32112.0
36	41715.5
37	50771.0
38	58718.0
39	65672.5
40	70729.0
41	76101.5
42	82651.0
43	88982.5
44	95072.0
45	100815.5
46	105396.5
47	109868.5
48	114343.0
49	116434.5
50	114723.5
51	112805.0
52	111426.0
53	109146.0
54	106335.5
55	100934.5
56	92995.5
57	83475.5
58	74702.5
59	65035.5
60	53401.5
61	43807.5
62	35657.0
63	27230.0
64	19661.5
65	14178.0
66	10542.0
67	8112.0
68	6189.0
69	4582.5
70	3431.0
71	2447.0
72	1700.0
73	1244.5
74	888.5
75	593.5
76	407.0
77	258.5
78	155.5
79	95.5
80	62.0
81	37.5
82	20.0
83	14.5
84	11.5
85	5.5
86	5.5
87	6.5
88	7.5
89	5.0
90	1.5
91	2.5
92	2.5
93	2.5
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16784812384919431
2	4.0001936093706936E-5
3	0.010480507256551218
4	0.007360356241242076
5	0.02664128943840882
6	0.0024001161656224163
7	0.0
8	0.0
9	4.0001936093706936E-5
10-11	0.04954239785205604
12-13	2.0000968046853467E-4
14-15	0.005080245883900781
16-17	0.0
18-19	0.00196009486859164
20-21	1.200058082811208E-4
22-23	2.0000968046853468E-5
24-25	6.40030977499311E-4
26-27	0.002820136494606339
28-29	0.005260254596322462
30-31	0.016220785085998165
32-33	0.011900575987877814
34-35	0.010760520809207166
36-37	0.004280207162026642
38-39	2.0000968046853468E-5
40-41	0.00352017037624621
42-43	0.010280497576082683
44-45	0.007540364953663758
46-47	0.0039001887691364266
48-49	0.008840427876709233
50-51	0.002580124878044097
52-53	0.002300111325388149
54-55	0.004380212002260909
56-57	0.009160443365458888
58-59	0.016300788958185576
60-61	0.008140393995069361
62-63	0.022461087116616443
64-65	0.04166201644159577
66-67	0.06094294963876252
68-69	0.05130248304017914
70-71	0.06912334556992558
72-73	0.034321661168400554
74-75	0.028101360105829122
76-77	0.2293911025293624
78-79	0.08598416163342305
80-81	0.0677432787746927
82-83	0.028421375594578774
84-85	0.0518825111135379
86-87	0.06968337267523748
88-89	0.29669436000702437
90-91	0.30017452844717685
92-93	0.05434263018330088
94-95	0.009700469502723932
96-97	0.01460070667420303
98-99	9.600464662489664E-4
100-101	2.2001064851538812E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2499879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.15683970076118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80710590484806	52.65135646124618
2	11.194632340315517	14.588137298137902
3	3.41671731882481	6.6786750793644805
4	1.5148421620853934	3.9480931170780984
5	0.8123263303406085	2.6464308245355306
6	0.4976148110510676	1.9453805085827385
7	0.3445367471987066	1.5714247925773461
8	0.24315014942690644	1.2674316247540058
9	0.18832077909498887	1.1043348134233182
>10	0.9339315488382823	10.643713844793856
>50	0.03619473259467924	1.5592677022505714
>100	0.009950023379915405	1.0537353091280528
>500	5.540334553874018E-4	0.2423851935748388
>1k	1.231185456397727E-4	0.09963343055312368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0001936093706936E-5
2	0.0	0.0	0.0	0.0	4.0001936093706936E-5
3	0.0	0.0	0.0	0.0	4.0001936093706936E-5
4	0.0	0.0	0.0	0.0	4.0001936093706936E-5
5	0.0	0.0	0.0	0.0	4.0001936093706936E-5
6	0.0	0.0	0.0	0.0	4.0001936093706936E-5
7	0.0	0.0	0.0	0.0	4.0001936093706936E-5
8	0.0	0.0	0.0	0.0	4.0001936093706936E-5
9	0.0	0.0	0.0	0.0	8.000387218741387E-5
10-11	0.0	0.0	0.0	6.000290414056041E-5	8.000387218741387E-5
12-13	0.0	0.0	0.0	8.000387218741387E-5	3.00014520702802E-4
14-15	0.0	2.0000968046853468E-5	0.0	8.000387218741387E-5	4.0001936093706934E-4
16-17	0.0	4.0001936093706936E-5	0.0	8.000387218741387E-5	5.200251692181902E-4
18-19	0.0	4.0001936093706936E-5	0.0	8.000387218741387E-5	5.200251692181902E-4
20-21	0.0	4.0001936093706936E-5	0.0	1.0000484023426734E-4	6.000290414056041E-4
22-23	0.0	4.0001936093706936E-5	0.0	1.8000871242168122E-4	6.200300094524575E-4
24-25	0.0	4.0001936093706936E-5	0.0	2.600125846090951E-4	7.200348496867249E-4
26-27	0.0	4.0001936093706936E-5	0.0	5.200251692181902E-4	7.600367857804318E-4
28-29	0.0	4.0001936093706936E-5	0.0	0.0016000774437482774	7.600367857804318E-4
30-31	0.0	4.0001936093706936E-5	0.0	0.006780328167883326	8.200396899209921E-4
32-33	0.0	4.0001936093706936E-5	0.0	0.02048099127997795	8.800425940615526E-4
34-35	0.0	4.0001936093706936E-5	0.0	0.03986192931737896	9.600464662489664E-4
36-37	0.0	4.0001936093706936E-5	0.0	0.07100343656632982	9.600464662489664E-4
38-39	0.0	4.0001936093706936E-5	0.0	0.12506605319697472	0.0010000484023426734
40-41	0.0	4.0001936093706936E-5	0.0	0.18986918966877997	0.0010400503384363803
42-43	0.0	4.0001936093706936E-5	0.0	0.26067261655464125	0.0010400503384363803
44-45	0.0	4.0001936093706936E-5	0.0	0.34921690209806155	0.0010400503384363803
46-47	0.0	4.0001936093706936E-5	0.0	0.4519618749547478	0.0010400503384363803
48-49	0.0	4.0001936093706936E-5	0.0	0.5586470385166642	0.0010800522745300872
50-51	0.0	4.0001936093706936E-5	0.0	0.6789528613184879	0.0010800522745300872
52-53	0.0	4.0001936093706936E-5	0.0	0.8032388767616352	0.0010800522745300872
54-55	0.0	4.0001936093706936E-5	0.0	0.9422056027511732	0.0011000532425769407
56-57	0.0	4.0001936093706936E-5	0.0	1.1038934284419366	0.0011200542106237941
58-59	0.0	4.0001936093706936E-5	0.0	1.2805019762956529	0.0011200542106237941
60-61	0.0	4.0001936093706936E-5	0.0	1.4788915783523922	0.0011600561467175012
62-63	0.0	4.0001936093706936E-5	0.0	1.6793612810860046	0.0012000580828112081
64-65	0.0	4.0001936093706936E-5	0.0	1.8939116653246018	0.0012200590508580616
66-67	0.0	4.0001936093706936E-5	0.0	2.145863859810815	0.001240060018904915
68-69	0.0	4.0001936093706936E-5	0.0	2.4188170707462238	0.001240060018904915
70-71	0.0	4.0001936093706936E-5	0.0	2.7094911393711456	0.001240060018904915
72-73	0.0	4.0001936093706936E-5	0.0	3.020086172170733	0.0014800716354671567
74-75	0.0	4.0001936093706936E-5	0.0	3.3537823230644364	0.0015600755076545705
76-77	0.0	4.0001936093706936E-5	0.0	3.717579930868654	0.001580076475701424
78-79	0.0	4.0001936093706936E-5	0.0	4.122279518328687	0.0016400793798419843
80-81	0.0	4.0001936093706936E-5	0.0	4.552300331336037	0.001720083252029398
82-83	0.0	4.0001936093706936E-5	0.0	5.005102246948752	0.0018600900283573724
84-85	0.0	4.0001936093706936E-5	0.0	5.483225388108785	0.001880090996404226
86-87	0.0	4.0001936093706936E-5	0.0	5.9936300916964385	0.00196009486859164
88-89	0.0	4.0001936093706936E-5	0.0	6.543296695560064	0.0020400987407790537
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1520	0.0	45.3469	1
GTATCAA	3170	0.0	45.28672	1
TCAACGC	3920	0.0	35.958916	4
CAACGCA	4105	0.0	34.25152	5
TATCAAC	4185	0.0	33.679928	2
ATCAACG	4190	0.0	33.52647	3
AACGCAG	4445	0.0	32.05649	6
GTACATG	7585	0.0	26.572563	1
ACGCAGA	5585	0.0	25.086956	7
TACATGG	7860	0.0	24.755487	2
CGCAGAG	5970	0.0	23.548676	8
ACATGGG	8165	0.0	23.540136	3
CATGGGG	6570	0.0	20.949268	4
TCTATAC	710	0.0	19.384285	3
GCAGAGT	7025	0.0	19.13442	9
TATACTG	930	0.0	18.898186	5
TAGATCG	135	0.009402439	17.592903	5
AGAGTAC	5825	0.0	17.082968	10-11
ATGGGGG	3505	0.0	17.075909	5
GTATATG	920	0.0	17.050978	1
>>END_MODULE