FastQCFastQC Report
Mon 15 Aug 2016
SRR2921698_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921698_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380109
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.043.4065551
GTATCAA41600.038.677981
TCAACGC43250.035.337844
CAACGCA45400.033.661865
ATCAACG46750.032.9973033
TATCAAC50050.030.9169522
AACGCAG50250.030.7752136
GTACATG77250.026.2353461
ACGCAGA58150.025.6183387
TACATGG80450.024.190282
CGCAGAG61000.023.9554718
ACATGGG85000.022.6158433
CATGGGG71800.021.4185894
GTGTAGC21000.020.793421
GTATATA11800.019.7093141
GTATATG12850.018.8375531
GCAGAGT75350.018.3863819
ATGGGGG43050.018.190685
GAGTACA58650.017.8037761
GTATAGG10000.017.56141
Produced by FastQC (version 0.11.5)