FastQCFastQC Report
Mon 15 Aug 2016
SRR2921698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380109
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.040.480781
GTATCAA38200.039.1970941
TCAACGC43800.033.590194
CAACGCA45650.032.358275
ATCAACG47300.031.0038623
AACGCAG50300.029.4565266
TATCAAC50250.029.1837372
GTACATG80900.025.2066691
TACATGG84550.023.6312642
ACGCAGA62100.023.6295437
CGCAGAG64400.022.1219738
ACATGGG86400.022.0816123
CATGGGG74100.019.4706424
GAGTACA62450.018.1916521
TATACCG3701.4188117E-1017.9718155
GCAGAGT76550.017.8045739
TATATAC12750.017.4946863
GTATATA12150.017.2140481
ATGGGGG41600.017.0121255
AGAGTAC62700.017.00371210-11
Produced by FastQC (version 0.11.5)