##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921698_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3380109
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.391369627429174	33.0	31.0	34.0	29.0	34.0
2	31.527008744392564	34.0	31.0	34.0	28.0	34.0
3	31.59899281354536	34.0	31.0	34.0	28.0	34.0
4	35.0776261948949	37.0	35.0	37.0	32.0	37.0
5	34.83067291616927	37.0	35.0	37.0	32.0	37.0
6	34.80905438256577	37.0	35.0	37.0	32.0	37.0
7	34.69439979598291	37.0	35.0	37.0	32.0	37.0
8	34.64455524954965	37.0	35.0	37.0	32.0	37.0
9	36.23670360926231	39.0	37.0	39.0	32.0	39.0
10-11	36.128647182679615	39.0	37.0	39.0	32.0	39.0
12-13	36.05406023888578	39.0	37.0	39.0	32.0	39.0
14-15	37.303048511157485	40.0	38.0	41.0	32.0	41.0
16-17	37.27211149107913	40.0	38.0	41.0	31.0	41.0
18-19	37.22857384184948	40.0	38.0	41.0	31.0	41.0
20-21	37.17264635548735	40.0	38.0	41.0	31.0	41.0
22-23	37.085932435906656	40.0	38.0	41.0	31.0	41.0
24-25	37.00621888229048	40.0	37.0	41.0	31.0	41.0
26-27	36.88583593014308	40.0	37.0	41.0	30.0	41.0
28-29	36.769417643040505	40.0	37.0	41.0	30.0	41.0
30-31	36.62283775464046	40.0	37.0	41.0	30.0	41.0
32-33	36.580504800288985	40.0	37.0	41.0	30.0	41.0
34-35	36.70970048599024	40.0	37.0	41.0	30.0	41.0
36-37	36.71459692572044	40.0	37.0	41.0	30.0	41.0
38-39	36.61678144107187	40.0	37.0	41.0	30.0	41.0
40-41	36.544650926937564	40.0	37.0	41.0	30.0	41.0
42-43	36.436680000556194	40.0	37.0	41.0	30.0	41.0
44-45	36.32002133659003	40.0	36.5	41.0	29.5	41.0
46-47	36.17409793589496	40.0	36.0	41.0	28.5	41.0
48-49	36.042153522268066	40.0	36.0	41.0	28.0	41.0
50-51	35.898560815642334	40.0	35.5	41.0	28.0	41.0
52-53	35.73857588616225	39.5	35.0	41.0	28.0	41.0
54-55	35.53535596041429	39.0	35.0	41.0	27.0	41.0
56-57	35.3235477021599	39.0	35.0	41.0	27.0	41.0
58-59	35.08793843630487	39.0	35.0	41.0	26.0	41.0
60-61	34.820184497008825	38.0	35.0	40.0	26.0	41.0
62-63	34.5282511599478	38.0	34.0	40.0	26.0	41.0
64-65	34.20361399588002	37.0	34.0	40.0	26.0	41.0
66-67	33.860107026134365	37.0	34.0	39.5	25.0	41.0
68-69	33.50177878879054	36.0	34.0	39.0	24.5	41.0
70-71	33.10606995218201	35.5	33.5	39.0	23.5	40.0
72-73	32.740174355324044	35.0	33.0	38.0	23.0	40.0
74-75	32.333307890366854	35.0	33.0	37.0	22.0	39.0
76-77	29.607057050527068	33.0	29.0	34.5	17.5	36.5
78-79	31.17541638450121	34.5	31.5	36.0	19.5	38.0
80-81	31.34203467994671	35.0	32.0	36.0	20.0	37.0
82-83	31.155898079026446	35.0	32.0	35.0	19.5	37.0
84-85	30.9423452024772	35.0	32.0	35.0	18.5	36.5
86-87	30.72321099704181	35.0	32.0	35.0	17.5	36.0
88-89	30.44393095015575	35.0	32.0	35.0	11.5	36.0
90-91	30.23405177170322	34.5	32.0	35.0	7.5	35.5
92-93	30.068458295279825	34.0	31.0	35.0	4.5	35.0
94-95	29.954882963833413	34.0	31.0	35.0	2.0	35.0
96-97	29.74646128275745	34.0	31.0	35.0	2.0	35.0
98-99	29.54131627116167	34.0	31.0	35.0	2.0	35.0
100-101	28.42848351931846	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61584.0
3	20728.0
4	11162.0
5	8038.0
6	6801.0
7	6862.0
8	7094.0
9	7756.0
10	8664.0
11	9471.0
12	10028.0
13	10487.0
14	11427.0
15	12244.0
16	12710.0
17	13530.0
18	13074.0
19	12126.0
20	11708.0
21	11769.0
22	12651.0
23	14573.0
24	16971.0
25	20756.0
26	24528.0
27	29657.0
28	35867.0
29	43471.0
30	52945.0
31	65464.0
32	83963.0
33	110782.0
34	155717.0
35	233513.0
36	407333.0
37	799727.0
38	890104.0
39	114821.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.01875452104953	19.565635489538725	13.053516483809569	26.362093505602175
2	15.198137335970333	21.134550553902912	37.36771490826468	26.299597201862074
3	17.886763646369772	24.74547635990289	29.193030835166557	28.17472915856078
4	12.873816643633434	16.87068163871169	36.044952822554755	34.21054889510012
5	13.978991326827591	37.391762897974154	34.749210702545014	13.880035072653243
6	33.415886694697924	36.120739542419074	16.89662476967701	13.56674899320599
7	28.738185662060012	31.68294276900538	21.262835015083834	18.31603655385078
8	26.498139557037952	35.40468665359609	19.751315712008104	18.34585807735786
9	26.370333027721887	15.408793030047255	19.192102976560815	39.02877096567004
10-11	25.461544381430485	25.502968825848615	28.1509513266478	20.8845354660731
12-13	25.960223638486223	22.710125564406713	27.901513506413128	23.428137290693936
14-15	23.019678032412738	24.396967221129746	24.93060150686263	27.652753239594887
16-17	23.547583820521766	27.518091872185185	25.622753585757145	23.311570721535904
18-19	23.472964711071967	27.00892840633191	26.774124500063536	22.74398238253259
20-21	24.022487517002098	26.15968918056358	26.706626066259926	23.111197236174398
22-23	23.75171924929048	26.067946329541442	26.42947313237532	23.750861288792755
24-25	23.600635131580933	26.204251547371417	26.46363836197116	23.731474959076497
26-27	23.553234448615555	26.377053940736328	26.441669392261204	23.628042218386913
28-29	23.617554400760255	26.561547683433552	26.247900835405375	23.572997080400814
30-31	23.926338343197123	26.258302552453255	26.208088514004746	23.60727059034488
32-33	23.666411070967676	26.314866778323964	26.13305997464558	23.88566217606278
34-35	23.891711756440365	26.343957329249392	26.27923339642499	23.485097517885258
36-37	23.95752973145132	26.277287823585127	26.23350038143892	23.53168206352463
38-39	23.838033033845953	26.355008078141857	26.223104047828045	23.58385484018415
40-41	23.975676230611604	26.182031523207495	26.21731273443145	23.62497951174946
42-43	23.784989358083944	26.093679336516068	26.190519515160016	23.93081179023997
44-45	23.750873925954988	26.188369173543425	26.26246981016686	23.79828709033473
46-47	23.866569743102257	26.166847584736374	26.078459566216754	23.88812310594462
48-49	23.625758626566366	26.22617013117967	26.218935958189142	23.929135284064817
50-51	23.772624038629523	26.24032712372805	26.175918944510595	23.81112989313183
52-53	23.883737718304275	26.158362499741127	26.12737165054748	23.830528131407117
54-55	23.51594485381788	26.068192482637382	26.39221979418703	24.023642869357705
56-57	23.596437945267937	26.18429177415427	26.241542886408766	23.977727394169026
58-59	23.678036474203708	26.079804260511764	26.283689697638234	23.95846956764629
60-61	23.706722026100852	26.11261175232107	26.33657129610576	23.844094925472316
62-63	23.686737087309	26.10661872464294	26.31824166680106	23.888402521247
64-65	23.447662388822774	25.95587036182562	26.31070814540506	24.285759103946543
66-67	23.40765526174357	26.13560657181946	26.17631077526114	24.28042739117583
68-69	23.54846372260057	26.05454837418258	26.217452016537525	24.17953588667933
70-71	23.476945481502664	25.866425322112235	26.225851666948657	24.430777529436444
72-73	23.44527556433534	26.04356350668472	26.11975525122263	24.39140567775731
74-75	23.363252207960272	26.075689361973502	26.14415244347505	24.41690598659118
76-77	23.395851338005688	25.817428804540164	26.272432728317035	24.51428712913711
78-79	23.36470349607176	25.861766475997864	26.24312381973248	24.530406208197896
80-81	23.44358195522556	26.012220899870332	26.025528299317312	24.5186688455868
82-83	23.34846170998646	26.012206177781334	26.15255044070466	24.486781671527545
84-85	23.351173893886973	25.98864445829407	26.104100211034943	24.556081436784016
86-87	23.23974799502811	26.05980151818178	26.08162051494905	24.61882997184106
88-89	23.195101394830765	25.921859865342956	26.10070334221443	24.78233539761185
90-91	23.107767675928454	25.922741623443674	26.149810689233615	24.81968001139425
92-93	23.18444783343832	25.85970394191559	26.216174854717938	24.739673369928152
94-95	23.087773833231033	25.70635322535858	26.257115310901934	24.948757630508453
96-97	22.953644964841256	25.706218826751858	26.24823555328507	25.091900655121812
98-99	22.807175591038057	25.62756096767227	26.22683849742757	25.33842494386211
100-101	22.782899090885152	25.686495758558692	26.229023925616424	25.301581224939728
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	26.0
2	38.0
3	64.5
4	60.0
5	55.0
6	102.0
7	221.5
8	357.0
9	388.5
10	297.0
11	237.0
12	277.5
13	300.5
14	312.5
15	367.5
16	410.5
17	456.5
18	549.5
19	627.5
20	708.0
21	850.5
22	1023.0
23	1252.5
24	1600.5
25	2075.5
26	2788.5
27	3905.0
28	5630.5
29	8474.5
30	12713.0
31	18733.0
32	27029.0
33	35238.5
34	43093.0
35	52026.0
36	62919.0
37	73767.5
38	83493.0
39	92733.5
40	100489.0
41	108186.5
42	115388.0
43	122907.5
44	131215.5
45	138888.0
46	144208.5
47	148412.5
48	152327.0
49	153347.0
50	151271.0
51	148369.0
52	145159.5
53	141647.0
54	136840.5
55	127793.5
56	115954.0
57	103279.5
58	92049.5
59	80268.0
60	66113.5
61	53725.0
62	43658.5
63	33143.5
64	23428.0
65	17024.5
66	12851.0
67	9835.0
68	7382.0
69	5544.0
70	4238.0
71	3007.0
72	2098.5
73	1567.5
74	1131.5
75	812.0
76	551.0
77	329.5
78	177.0
79	97.5
80	57.0
81	34.0
82	21.5
83	16.5
84	12.0
85	7.5
86	6.5
87	5.0
88	3.0
89	2.5
90	2.5
91	2.5
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16614848811088637
2	1.1833937899635781E-4
3	0.010768883488668562
4	0.006212817397308785
5	0.024792099899736963
6	0.00215969366668353
7	0.0
8	0.0
9	0.0
10-11	0.0493771058862303
12-13	3.25433292239984E-4
14-15	0.00510338571921793
16-17	0.0
18-19	0.0016123740388253752
20-21	3.4022571461452873E-4
22-23	0.0
24-25	6.50866584479968E-4
26-27	0.0033282950342725633
28-29	0.005709875036574265
30-31	0.017040870575475525
32-33	0.012499596906490293
34-35	0.010946392557163096
36-37	0.004275010066243427
38-39	0.0
40-41	0.003638935904138003
42-43	0.009274848828839544
44-45	0.007277871808276006
46-47	0.0037276904383852713
48-49	0.009378395785461355
50-51	0.003062031431530758
52-53	0.002337202735178067
54-55	0.00434897217811615
56-57	0.007751229324261436
58-59	0.015236195045781067
60-61	0.007618097522890534
62-63	0.022173841139442544
64-65	0.04088625544324162
66-67	0.06152168465573152
68-69	0.05122615868304839
70-71	0.0699829502539711
72-73	0.033549213945467435
74-75	0.027084925367791394
76-77	0.22840979388534513
78-79	0.08761551772442841
80-81	0.06771970963066576
82-83	0.027173679902038658
84-85	0.05202494949127381
86-87	0.0685924625507639
88-89	0.29121841928766207
90-91	0.29552301419865457
92-93	0.05482071732006275
94-95	0.00998488510281769
96-97	0.015043893554911987
98-99	0.0011538089452144886
100-101	2.3667875799271562E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3380109.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.924399997584636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81528845190427	50.223291364702206
2	11.530666068693908	14.51120487890144
3	3.644246914535284	6.879361516205454
4	1.6021378206795123	4.032542443187846
5	0.8771245728020889	2.7596268733354594
6	0.5615601499553133	2.1201501299095042
7	0.39053854987653047	1.7202082748835223
8	0.2652074315398214	1.3350414803635011
9	0.21484089307874354	1.2166860862732654
>10	1.0366810808101543	11.392715730931286
>50	0.04800794843206832	2.0228664144767237
>100	0.012851842444047079	1.3579823435980187
>500	7.068960413299434E-4	0.3242072485746683
>1k	1.413792068798923E-4	0.10411521465698736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.9169689498178905E-5
2	0.0	0.0	0.0	0.0	5.9169689498178905E-5
3	0.0	0.0	0.0	0.0	5.9169689498178905E-5
4	0.0	0.0	0.0	0.0	5.9169689498178905E-5
5	0.0	0.0	0.0	0.0	8.875453424726836E-5
6	0.0	0.0	0.0	0.0	1.4792422374544726E-4
7	0.0	0.0	0.0	0.0	1.4792422374544726E-4
8	0.0	0.0	0.0	0.0	1.775090684945367E-4
9	0.0	0.0	0.0	0.0	2.3667875799271562E-4
10-11	0.0	0.0	0.0	2.9584844749089452E-5	2.5147118036726037E-4
12-13	0.0	0.0	0.0	2.9584844749089452E-5	3.106408698654393E-4
14-15	0.0	0.0	0.0	2.9584844749089452E-5	3.698105593636182E-4
16-17	0.0	0.0	0.0	2.9584844749089452E-5	4.1418782648725233E-4
18-19	0.0	0.0	0.0	2.9584844749089452E-5	4.437726712363418E-4
20-21	0.0	0.0	0.0	2.9584844749089452E-5	5.325272054836101E-4
22-23	0.0	0.0	0.0	1.4792422374544726E-4	5.621120502326995E-4
24-25	0.0	0.0	0.0	2.5147118036726037E-4	6.212817397308786E-4
26-27	0.0	0.0	0.0	3.846029817381629E-4	6.212817397308786E-4
28-29	0.0	0.0	0.0	0.0020709391324362615	6.360741621054232E-4
30-31	0.0	0.0	0.0	0.00917130187221773	6.804514292290573E-4
32-33	0.0	0.0	0.0	0.026463643628060515	7.396211187272364E-4
34-35	0.0	0.0	0.0	0.05246872216251015	7.987908082254153E-4
36-37	0.0	0.0	0.0	0.09579572729755165	7.987908082254153E-4
38-39	0.0	0.0	0.0	0.16784961668395904	8.431680753490494E-4
40-41	0.0	0.0	0.0	0.253660458878693	8.727529200981388E-4
42-43	0.0	0.0	0.0	0.3467491728817029	9.17130187221773E-4
44-45	1.4792422374544726E-5	0.0	0.0	0.4536688018049122	9.467150319708625E-4
46-47	2.9584844749089452E-5	0.0	0.0	0.5823480840410767	9.910922990944968E-4
48-49	2.9584844749089452E-5	0.0	0.0	0.7199176121243427	0.0010354695662181308
50-51	2.9584844749089452E-5	0.0	0.0	0.8692027387282482	0.0010354695662181308
52-53	2.9584844749089452E-5	0.0	0.0	1.0230143465787642	0.0011094316780908544
54-55	2.9584844749089452E-5	0.0	0.0	1.19746138364177	0.0011242241004653993
56-57	2.9584844749089452E-5	0.0	0.0	1.4005909276890183	0.0011538089452144888
58-59	2.9584844749089452E-5	0.0	0.0	1.619814627279771	0.001183393789963578
60-61	2.9584844749089452E-5	0.0	0.0	1.8509462268820327	0.001183393789963578
62-63	2.9584844749089452E-5	0.0	0.0	2.0920035418976135	0.001183393789963578
64-65	5.9169689498178905E-5	0.0	0.0	2.3461521507146665	0.001183393789963578
66-67	5.9169689498178905E-5	0.0	0.0	2.6433467086416442	0.0011981862123381227
68-69	5.9169689498178905E-5	0.0	0.0	2.964800839262876	0.0012129786347126676
70-71	5.9169689498178905E-5	0.0	0.0	3.305440741703892	0.001286940746585391
72-73	5.9169689498178905E-5	0.0	0.0	3.6656806037911793	0.0014348649703308384
74-75	5.9169689498178905E-5	0.0	0.0	4.045934613351227	0.001508827082203562
76-77	5.9169689498178905E-5	0.0	0.0	4.465151863445824	0.001567996771701741
78-79	5.9169689498178905E-5	0.0	0.0	4.935891120670961	0.0016271664611999198
80-81	5.9169689498178905E-5	0.0	0.0	5.428286484252431	0.0016271664611999198
82-83	5.9169689498178905E-5	0.0	0.0	5.940740372573784	0.0016567513059490093
84-85	5.9169689498178905E-5	0.0	0.0	6.46544238662126	0.001671543728323554
86-87	5.9169689498178905E-5	0.0	0.0	7.02459595237905	0.001730713417821733
88-89	5.9169689498178905E-5	0.0	0.0	7.639709251979744	0.0017750906849453671
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2325	0.0	40.48078	1
GTATCAA	3820	0.0	39.197094	1
TCAACGC	4380	0.0	33.59019	4
CAACGCA	4565	0.0	32.35827	5
ATCAACG	4730	0.0	31.003862	3
AACGCAG	5030	0.0	29.456526	6
TATCAAC	5025	0.0	29.183737	2
GTACATG	8090	0.0	25.206669	1
TACATGG	8455	0.0	23.631264	2
ACGCAGA	6210	0.0	23.629543	7
CGCAGAG	6440	0.0	22.121973	8
ACATGGG	8640	0.0	22.081612	3
CATGGGG	7410	0.0	19.470642	4
GAGTACA	6245	0.0	18.191652	1
TATACCG	370	1.4188117E-10	17.971815	5
GCAGAGT	7655	0.0	17.804573	9
TATATAC	1275	0.0	17.494686	3
GTATATA	1215	0.0	17.214048	1
ATGGGGG	4160	0.0	17.012125	5
AGAGTAC	6270	0.0	17.003712	10-11
>>END_MODULE