FastQCFastQC Report
Mon 15 Aug 2016
SRR2921697_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921697_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096483
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.046.8269961
GTATCAA35750.045.143161
TCAACGC40150.038.8955044
CAACGCA42250.036.9586525
ATCAACG43200.036.588923
AACGCAG45100.034.4983836
TATCAAC46950.033.667032
ACGCAGA53150.028.7401567
CGCAGAG56550.026.8461448
GTACATG69950.025.3110521
TACATGG72350.022.7004092
GCAGAGT66500.021.3321749
ACATGGG75850.021.0893613
CATGGGG60350.019.3484844
AGAGTAC54900.018.8830310-11
GAGTACA52600.018.31891
GTATAGG9550.017.8932081
TAAGGCG11200.017.7982885
CAGAGTA61500.017.51230610-11
GTATAGA8450.017.4138221
Produced by FastQC (version 0.11.5)