##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921697_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3096483 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.224956507108224 31.0 31.0 34.0 26.0 34.0 2 30.283865921434092 31.0 31.0 34.0 26.0 34.0 3 30.345757105722846 33.0 31.0 34.0 26.0 34.0 4 33.790662180286475 37.0 35.0 37.0 28.0 37.0 5 33.791205700144324 37.0 35.0 37.0 28.0 37.0 6 33.829048310615626 37.0 35.0 37.0 29.0 37.0 7 33.73874909050041 37.0 35.0 37.0 29.0 37.0 8 33.710079467576605 37.0 35.0 37.0 28.0 37.0 9 35.257933597568595 39.0 35.0 39.0 29.0 39.0 10-11 35.15534172155959 39.0 35.0 39.0 28.0 39.0 12-13 35.04903401697991 39.0 35.0 39.0 27.0 39.0 14-15 36.17787793441785 40.0 36.0 41.0 27.0 41.0 16-17 36.11995480033315 40.0 36.0 41.0 27.0 41.0 18-19 36.090063953201096 40.0 36.5 41.0 27.0 41.0 20-21 36.01240923977299 40.0 36.0 41.0 27.0 41.0 22-23 35.915468936855135 40.0 36.0 41.0 27.0 41.0 24-25 35.8065784633728 40.0 36.0 41.0 27.0 41.0 26-27 35.667923576522135 40.0 36.0 41.0 26.5 41.0 28-29 35.53282433651339 40.0 36.0 41.0 25.5 41.0 30-31 35.38967499579361 40.0 36.0 41.0 25.0 41.0 32-33 35.21674154193644 39.0 35.0 41.0 24.5 41.0 34-35 35.04917385304554 39.0 35.0 41.0 24.0 41.0 36-37 35.01236128213848 39.0 35.0 41.0 24.0 41.0 38-39 35.19152067038637 39.5 35.0 41.0 24.0 41.0 40-41 35.2572300574555 40.0 35.0 41.0 24.0 41.0 42-43 35.10271217377909 40.0 35.0 41.0 23.0 41.0 44-45 34.9909109786813 40.0 35.0 41.0 23.0 41.0 46-47 34.84574095837116 40.0 35.0 41.0 22.0 41.0 48-49 34.68788735478283 39.5 35.0 41.0 20.5 41.0 50-51 33.01789933934725 37.0 32.5 39.5 16.5 40.0 52-53 33.13492565597809 37.5 33.0 39.5 15.0 40.0 54-55 34.05192923713775 39.0 34.0 40.5 17.5 41.0 56-57 34.085985939532044 39.0 34.0 41.0 16.0 41.0 58-59 33.91117212657069 38.5 34.0 41.0 14.0 41.0 60-61 33.67533924778531 38.0 34.0 40.5 13.0 41.0 62-63 33.39974157778357 37.5 34.0 40.0 10.0 41.0 64-65 33.12603702329385 37.0 33.0 40.0 8.0 41.0 66-67 32.79632618683842 36.5 33.0 39.5 7.0 41.0 68-69 32.44918751370506 36.0 33.0 39.0 2.0 41.0 70-71 32.04764728241686 35.5 32.0 39.0 2.0 41.0 72-73 31.692887220759808 35.0 32.0 38.5 2.0 40.0 74-75 31.29395252614014 35.0 32.0 37.0 2.0 39.5 76-77 30.918261621329748 35.0 32.0 37.0 2.0 39.0 78-79 30.570237265956248 35.0 31.0 36.0 2.0 39.0 80-81 30.249643708684985 35.0 31.0 36.0 2.0 37.0 82-83 29.933428021403635 35.0 31.0 35.5 2.0 37.0 84-85 29.668292059087683 35.0 31.0 35.0 2.0 36.5 86-87 29.4323396898998 35.0 31.0 35.0 2.0 36.0 88-89 29.21163623375294 35.0 30.5 35.0 2.0 36.0 90-91 28.992809422819374 34.5 30.0 35.0 2.0 36.0 92-93 28.80789883232041 34.0 30.0 35.0 2.0 35.0 94-95 28.589305189145232 34.0 30.0 35.0 2.0 35.0 96-97 28.366223712515133 34.0 30.0 35.0 2.0 35.0 98-99 28.11477650612001 34.0 29.5 35.0 2.0 35.0 100-101 27.058066361094184 33.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 117077.0 3 19442.0 4 12998.0 5 9690.0 6 8789.0 7 9030.0 8 9494.0 9 10155.0 10 11162.0 11 11893.0 12 11869.0 13 12117.0 14 12784.0 15 13419.0 16 13332.0 17 13896.0 18 13208.0 19 12748.0 20 12768.0 21 13398.0 22 14664.0 23 16731.0 24 19675.0 25 23250.0 26 27250.0 27 32766.0 28 38316.0 29 45237.0 30 54340.0 31 66280.0 32 81915.0 33 106005.0 34 145890.0 35 211457.0 36 352072.0 37 641451.0 38 731555.0 39 138349.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.856068160213205 18.159793151537436 12.857556450669602 25.126582237579754 2 15.845213809793442 19.611124469644672 39.3043716016537 25.239290118908187 3 18.93799423904195 23.76086562385979 29.524118235051354 27.777021902046904 4 12.9741723938899 15.897011459186384 37.374179374498674 33.754636772425044 5 14.115805420833292 37.09381883331093 34.913547946763735 13.876827799092048 6 33.315343353461444 35.67471144976629 17.399604930998578 13.61034026577369 7 28.657551856230178 31.651098492923264 21.51259988501515 18.178749765831412 8 26.457020189654717 34.68937725577546 20.31711188387896 18.53649067069086 9 26.5577013942874 15.449296041665523 19.551093135400997 38.441909428646085 10-11 25.30099331994437 25.26569063739268 28.454559666055356 20.978756376607596 12-13 25.97185586304961 22.71821493660075 27.904487983311466 23.405441217038174 14-15 23.072162680262124 24.355871796441612 25.168617263325622 27.403348259970645 16-17 23.444078068052328 27.236420989894633 25.975741629537747 23.343759312515296 18-19 23.37916044862589 26.792446979982177 27.046729252683683 22.781663318708254 20-21 23.97586185622222 26.05610031761906 26.976510790100416 22.991527036058308 22-23 23.732922782287766 26.022339300990648 26.567463086589182 23.677274830132404 24-25 23.544030138725947 26.083411311455524 26.72025552594729 23.65230302387124 26-27 23.464783145602492 26.252223553402267 26.6016771576503 23.68131614334494 28-29 23.589241722119002 26.4083762700341 26.459859937938603 23.542522069908298 30-31 23.795947712756064 26.19898143745995 26.386473685842443 23.618597163941544 32-33 23.499214606970014 26.266588571453486 26.33643178836908 23.89776503320742 34-35 23.9678451118915 26.264018430878792 26.304340418990186 23.463796038239522 36-37 24.012211356057335 25.999527848234532 26.352091516059296 23.63616927964884 38-39 23.717007992621003 26.181098039114364 26.399225412065018 23.70266855619961 40-41 24.021666098004797 26.027866423183575 26.41075644516197 23.539711033649656 42-43 23.753125773300656 26.002015578574145 26.316134452394298 23.9287241957309 44-45 23.77436970092435 26.057355852764996 26.407058644222953 23.7612158020877 46-47 23.951254911764206 26.073703363016286 26.13180719752958 23.843234527689933 48-49 23.688620432870227 26.15655420360247 26.226135000338196 23.928690363189105 50-51 23.781633739116984 26.169995503517825 26.283985129086577 23.764385628278617 52-53 23.98000247897804 26.003622026399498 26.281730352561812 23.73464514206065 54-55 23.611011105309426 25.956211428211756 26.53902456507454 23.893752901404277 56-57 23.68163900530757 26.09089486623147 26.407057817253964 23.820408311206997 58-59 23.68983738278344 25.94211390891889 26.4770293815937 23.891019326703976 60-61 23.65809296828562 25.958486527565576 26.640074429780846 23.74334607436796 62-63 23.676020425499967 25.99785311542595 26.54889437386147 23.777232085212614 64-65 23.494105935875638 25.878786093982704 26.48659021934216 24.1405177507995 66-67 23.50045710124995 25.962266898588243 26.448266655879614 24.08900934428219 68-69 23.719001230954824 25.853222551713696 26.464816825896907 23.962959391434573 70-71 23.597805872631568 25.684880145671162 26.44965373978025 24.26766024191702 72-73 23.529089110429595 25.811061955350777 26.467707189588168 24.192141744631456 74-75 23.529176676780335 25.92535842247512 26.423561132454516 24.121903768290032 76-77 23.63701344906373 25.69121288790678 26.457068192768514 24.21470547026098 78-79 23.554765084901877 25.715525714587162 26.51253567717124 24.21717352333972 80-81 23.697720523381427 25.801005008860585 26.399527435447194 24.10174703231079 82-83 23.685801200723663 25.79388898751875 26.4812860499098 24.039023761847787 84-85 23.642722214787224 25.80337222385756 26.480402972222755 24.073502589132456 86-87 23.631683890746743 25.80318208870478 26.461457084500527 24.103676936047943 88-89 23.55266514057244 25.710258538315927 26.493638828394673 24.243437492716957 90-91 23.538843246216157 25.709874889674467 26.56344205621378 24.187839807895596 92-93 23.653662703618856 25.491334737539933 26.71990221698261 24.135100341858596 94-95 23.515331360216962 25.454172305953243 26.75017504582915 24.280321288000646 96-97 23.61096340884844 25.280816397751426 26.811513622033388 24.296706571366737 98-99 23.494291912437955 25.286925822619967 26.698049427200417 24.520732837741665 100-101 23.630375515398296 25.170828242005612 26.84536990920508 24.353426333391013 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 30.0 1 25.0 2 40.0 3 61.5 4 59.0 5 64.0 6 101.5 7 219.0 8 371.5 9 426.0 10 410.0 11 423.5 12 465.0 13 539.0 14 625.5 15 701.0 16 810.5 17 910.0 18 1050.0 19 1160.0 20 1250.0 21 1411.5 22 1536.5 23 1732.0 24 2095.0 25 2532.5 26 3253.5 27 4450.0 28 6217.5 29 9078.5 30 13176.0 31 18960.5 32 26936.0 33 33953.0 34 40191.0 35 47848.0 36 57578.0 37 67764.0 38 76752.0 39 85025.5 40 92605.0 41 100595.5 42 107632.0 43 113700.5 44 119329.0 45 125294.0 46 130239.0 47 132799.5 48 135288.0 49 136540.0 50 134827.5 51 132413.0 52 129653.0 53 126528.0 54 122296.0 55 115128.5 56 106241.5 57 95871.5 58 86025.0 59 74021.5 60 59983.0 61 49213.5 62 40290.5 63 30728.0 64 22215.5 65 16451.0 66 12353.5 67 9490.5 68 7202.0 69 5408.5 70 4129.0 71 3033.5 72 2163.0 73 1634.5 74 1266.5 75 956.5 76 704.0 77 470.5 78 315.0 79 224.5 80 168.5 81 138.5 82 118.5 83 86.0 84 65.5 85 55.5 86 49.5 87 44.0 88 42.5 89 41.5 90 34.5 91 28.5 92 19.5 93 15.0 94 15.5 95 20.0 96 16.5 97 15.0 98 15.0 99 8.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05351232349733552 2 0.07527895357410326 3 0.07124211565185405 4 0.07095146332145211 5 0.06623643662826503 6 0.010172831564068008 7 0.013660659528891327 8 0.01747143452749458 9 0.006652708895866698 10-11 0.012950176054575465 12-13 0.022767765881485543 14-15 0.039835516616755204 16-17 0.027466645222983622 18-19 0.010253568322512993 20-21 0.02053943134840398 22-23 0.03652530952051085 24-25 0.034345417042496273 26-27 0.02099155719569589 28-29 0.011480767050876754 30-31 0.011464619699187757 32-33 0.008477359636723341 34-35 0.005086415782034004 36-37 6.781887709378673E-4 38-39 0.004101427329005197 40-41 0.0010495778597847945 42-43 0.22759692205641044 44-45 0.19843480490608217 46-47 0.20428014621749901 48-49 0.2115303071258586 50-51 0.20311753689589127 52-53 0.2099155719569589 54-55 0.20618553371680062 56-57 0.20870452058028413 58-59 0.2177470375261224 60-61 0.22152551782134763 62-63 0.20746117450023138 64-65 0.2074934692036094 66-67 0.20532972407728384 68-69 0.20019486624018282 70-71 0.1924925794845313 72-73 0.20365039950162814 74-75 0.20264926369691033 76-77 0.19641638594495753 78-79 0.21385552576907416 80-81 0.22470654610408 82-83 0.2237861470578072 84-85 0.2177147428227444 86-87 0.23731762777318655 88-89 0.229776814534425 90-91 0.23670402840900465 92-93 0.23232809610128652 94-95 0.2354929770323299 96-97 0.2421618332798856 98-99 0.23618731315495678 100-101 0.24183888624610567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3096483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.13031125826573 #Duplication Level Percentage of deduplicated Percentage of total 1 78.49766970878206 49.555823217639485 2 12.258907987010698 15.4781735381285 3 4.059355310221276 7.68805092726489 4 1.798638516745326 4.541944376129513 5 0.966549076993381 3.0509272038490787 6 0.5976989417246089 2.2639752137886355 7 0.3935121646812637 1.7389781808169469 8 0.2686746934830824 1.3569213621444904 9 0.1941811591707806 1.1032845317048 >10 0.9190811908591747 9.812712954894236 >50 0.034306733371402824 1.440637525598076 >100 0.009784750500100267 1.035832727769692 >500 0.001127339439626694 0.5121924380239318 >1k 5.124270172474777E-4 0.4205458022477136 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.229470337799368E-5 2 0.0 0.0 0.0 3.229470337799368E-5 3.229470337799368E-5 3 0.0 0.0 0.0 3.229470337799368E-5 3.229470337799368E-5 4 0.0 0.0 0.0 3.229470337799368E-5 3.229470337799368E-5 5 0.0 9.688411013398104E-5 0.0 3.229470337799368E-5 3.229470337799368E-5 6 0.0 9.688411013398104E-5 0.0 3.229470337799368E-5 3.229470337799368E-5 7 0.0 9.688411013398104E-5 0.0 3.229470337799368E-5 3.229470337799368E-5 8 0.0 9.688411013398104E-5 0.0 3.229470337799368E-5 3.229470337799368E-5 9 3.229470337799368E-5 9.688411013398104E-5 0.0 6.458940675598736E-5 3.229470337799368E-5 10-11 3.229470337799368E-5 9.688411013398104E-5 0.0 6.458940675598736E-5 4.844205506699052E-5 12-13 3.229470337799368E-5 9.688411013398104E-5 0.0 6.458940675598736E-5 1.4532616520097154E-4 14-15 3.229470337799368E-5 9.688411013398104E-5 0.0 6.458940675598736E-5 2.5835762702394944E-4 16-17 3.229470337799368E-5 9.688411013398104E-5 0.0 6.458940675598736E-5 3.5524173715793046E-4 18-19 3.229470337799368E-5 9.688411013398104E-5 0.0 6.458940675598736E-5 4.198311439139178E-4 20-21 3.229470337799368E-5 1.4532616520097154E-4 0.0 9.688411013398104E-5 4.3597849560291467E-4 22-23 3.229470337799368E-5 1.6147351688996839E-4 0.0 1.2917881351197472E-4 4.844205506699052E-4 24-25 3.229470337799368E-5 1.6147351688996839E-4 0.0 2.5835762702394944E-4 5.328626057368957E-4 26-27 3.229470337799368E-5 1.6147351688996839E-4 0.0 5.328626057368957E-4 5.651573091148894E-4 28-29 3.229470337799368E-5 1.6147351688996839E-4 0.0 0.0021637451263255766 5.813046608038863E-4 30-31 3.229470337799368E-5 1.6147351688996839E-4 0.0 0.010996346500206847 5.813046608038863E-4 32-33 3.229470337799368E-5 1.6147351688996839E-4 0.0 0.030825294374294966 5.813046608038863E-4 34-35 6.458940675598736E-5 1.6147351688996839E-4 0.0 0.05804972932194363 6.135993641818799E-4 36-37 6.458940675598736E-5 1.7762086857896523E-4 0.0 0.10095324275960824 6.135993641818799E-4 38-39 6.458940675598736E-5 1.9376822026796208E-4 0.0 0.17548941815601765 6.297467158708767E-4 40-41 6.458940675598736E-5 2.0991557195695893E-4 0.0 0.25937491018035624 6.781887709378673E-4 42-43 6.458940675598736E-5 2.5835762702394944E-4 0.0 0.34925106968131264 6.943361226268641E-4 44-45 6.458940675598736E-5 2.5835762702394944E-4 0.0 0.45685702133678757 7.266308260048577E-4 46-47 6.458940675598736E-5 2.5835762702394944E-4 0.0 0.5801097567789004 7.912202327608451E-4 48-49 6.458940675598736E-5 2.7450497871294626E-4 0.0 0.7170070043982157 9.365463979618167E-4 50-51 6.458940675598736E-5 2.9065233040194313E-4 0.0 0.8629629163150581 9.365463979618167E-4 52-53 6.458940675598736E-5 2.9065233040194313E-4 0.0 1.0155715371277672 0.0010818725631627883 54-55 6.458940675598736E-5 3.5524173715793046E-4 0.0 1.1831649003078653 0.0011949040249857662 56-57 6.458940675598736E-5 3.5524173715793046E-4 0.0 1.3780472878423682 0.0012433460800527567 58-59 6.458940675598736E-5 3.5524173715793046E-4 0.0 1.5878821230408824 0.0013402301901867376 60-61 6.458940675598736E-5 4.198311439139178E-4 0.0 1.8088586309048038 0.0014371143003207186 62-63 6.458940675598736E-5 4.198311439139178E-4 0.0 2.0354867118598747 0.0014855563553877091 64-65 6.458940675598736E-5 4.198311439139178E-4 0.0 2.2803451528718224 0.0014855563553877091 66-67 6.458940675598736E-5 4.844205506699052E-4 0.0 2.558273370142836 0.0014855563553877091 68-69 6.458940675598736E-5 4.844205506699052E-4 0.0 2.865202231047288 0.0014855563553877091 70-71 6.458940675598736E-5 4.844205506699052E-4 0.0 3.1894733476657224 0.001501703707076706 72-73 6.458940675598736E-5 5.005679023589021E-4 0.0 3.5283901122660772 0.001808503389167646 74-75 6.458940675598736E-5 5.167152540478989E-4 0.0 3.8804185264378974 0.0018407980925456396 76-77 6.458940675598736E-5 5.167152540478989E-4 0.0 4.269618144197788 0.0018569454442346365 78-79 6.458940675598736E-5 5.167152540478989E-4 0.0 4.706468596791909 0.0018730927959236335 80-81 6.458940675598736E-5 5.167152540478989E-4 0.0 5.166538941114807 0.001937682202679621 82-83 6.458940675598736E-5 5.167152540478989E-4 0.0 5.647681579391845 0.002050713664502599 84-85 6.458940675598736E-5 5.167152540478989E-4 0.0 6.142420287791019 0.0020668610161915955 86-87 6.458940675598736E-5 5.167152540478989E-4 0.0 6.669647467788455 0.002083008367880592 88-89 6.458940675598736E-5 5.167152540478989E-4 0.0 7.237049258788115 0.002099155719569589 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1855 0.0 46.826996 1 GTATCAA 3575 0.0 45.14316 1 TCAACGC 4015 0.0 38.895504 4 CAACGCA 4225 0.0 36.958652 5 ATCAACG 4320 0.0 36.58892 3 AACGCAG 4510 0.0 34.498383 6 TATCAAC 4695 0.0 33.66703 2 ACGCAGA 5315 0.0 28.740156 7 CGCAGAG 5655 0.0 26.846144 8 GTACATG 6995 0.0 25.311052 1 TACATGG 7235 0.0 22.700409 2 GCAGAGT 6650 0.0 21.332174 9 ACATGGG 7585 0.0 21.089361 3 CATGGGG 6035 0.0 19.348484 4 AGAGTAC 5490 0.0 18.88303 10-11 GAGTACA 5260 0.0 18.3189 1 GTATAGG 955 0.0 17.893208 1 TAAGGCG 1120 0.0 17.798288 5 CAGAGTA 6150 0.0 17.512306 10-11 GTATAGA 845 0.0 17.413822 1 >>END_MODULE