FastQCFastQC Report
Mon 15 Aug 2016
SRR2921697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096483
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.042.451951
GTATCAA34450.040.0169221
TCAACGC40600.033.2011534
CAACGCA42350.031.849275
ATCAACG43000.031.4589563
AACGCAG45750.029.4766126
TATCAAC46700.029.2709262
GTACATG70150.025.9541021
TACATGG74400.024.0507872
ACGCAGA56800.023.324177
CGCAGAG59350.022.3220378
ACATGGG76800.021.939923
CATGGGG60800.019.0479014
GGACCGT4450.018.1400836
GCAGAGT74450.016.4552829
AGAGTAC59650.016.43841610-11
GTATTAA20150.016.0423771
GAGTACA52650.015.5297671
TATACTG13900.015.37560755
GTATTAG9300.015.3346261
Produced by FastQC (version 0.11.5)