##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3096483
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27038546635005	33.0	31.0	34.0	28.0	34.0
2	31.409699649570175	34.0	31.0	34.0	28.0	34.0
3	31.48409308237765	34.0	31.0	34.0	28.0	34.0
4	34.95673575472561	37.0	35.0	37.0	32.0	37.0
5	34.690805665653585	37.0	35.0	37.0	32.0	37.0
6	34.66228330657717	37.0	35.0	37.0	32.0	37.0
7	34.54675578713011	37.0	35.0	37.0	32.0	37.0
8	34.49037827754908	37.0	35.0	37.0	31.0	37.0
9	36.074749966332774	39.0	37.0	39.0	31.0	39.0
10-11	35.959282030613444	39.0	37.0	39.0	31.0	39.0
12-13	35.886112082643436	39.0	37.0	39.0	30.5	39.0
14-15	37.1250279752868	40.0	38.0	41.0	31.0	41.0
16-17	37.0881078630175	40.0	38.0	41.0	31.0	41.0
18-19	37.05020679913308	40.0	38.0	41.0	31.0	41.0
20-21	36.990281231965426	40.0	38.0	41.0	31.0	41.0
22-23	36.89914493313866	40.0	37.0	41.0	30.0	41.0
24-25	36.81663358074306	40.0	37.0	41.0	30.0	41.0
26-27	36.69631514204987	40.0	37.0	41.0	30.0	41.0
28-29	36.57569345609196	40.0	37.0	41.0	30.0	41.0
30-31	36.42136417348327	40.0	37.0	41.0	29.5	41.0
32-33	36.36936534125975	40.0	36.5	41.0	29.5	41.0
34-35	36.4992275106952	40.0	37.0	41.0	30.0	41.0
36-37	36.49285625013927	40.0	37.0	41.0	30.0	41.0
38-39	36.38996177275961	40.0	37.0	41.0	29.5	41.0
40-41	36.312083902931164	40.0	37.0	41.0	29.0	41.0
42-43	36.19100976817893	40.0	36.5	41.0	28.5	41.0
44-45	36.06673086853698	40.0	36.0	41.0	28.0	41.0
46-47	35.92067112914878	40.0	36.0	41.0	28.0	41.0
48-49	35.7824969489579	40.0	36.0	41.0	27.0	41.0
50-51	35.63126279072095	40.0	35.0	41.0	27.0	41.0
52-53	35.45835743325573	39.0	35.0	41.0	26.5	41.0
54-55	35.25640734988695	39.0	35.0	41.0	26.0	41.0
56-57	35.03855922993925	39.0	35.0	41.0	26.0	41.0
58-59	34.80983409242034	39.0	35.0	40.5	26.0	41.0
60-61	34.54612975430513	38.0	34.5	40.0	25.0	41.0
62-63	34.252622572124565	37.5	34.0	40.0	24.0	41.0
64-65	33.92373686534045	37.0	34.0	40.0	23.0	41.0
66-67	33.582265105282346	36.5	34.0	39.5	22.5	41.0
68-69	33.22562985167366	36.0	34.0	39.0	22.0	41.0
70-71	32.83023497949125	35.5	33.0	39.0	21.5	40.0
72-73	32.465161119889885	35.0	33.0	38.0	20.0	40.0
74-75	32.05182379493122	35.0	33.0	37.0	19.0	39.0
76-77	29.33833981972451	33.0	29.0	34.5	12.0	36.5
78-79	30.893448631883334	34.5	31.5	36.0	14.0	38.0
80-81	31.064505440527206	35.0	32.0	36.0	15.5	37.0
82-83	30.875198572057396	35.0	32.0	35.0	12.5	37.0
84-85	30.661271029099787	35.0	32.0	35.0	9.5	36.5
86-87	30.444110786334043	35.0	32.0	35.0	7.0	36.0
88-89	30.165993483574752	35.0	31.5	35.0	2.0	36.0
90-91	29.961712368516153	34.0	31.0	35.0	2.0	35.5
92-93	29.79780754488237	34.0	31.0	35.0	2.0	35.0
94-95	29.686407772947568	34.0	31.0	35.0	2.0	35.0
96-97	29.480391463476465	34.0	31.0	35.0	2.0	35.0
98-99	29.272946274854405	34.0	31.0	35.0	2.0	35.0
100-101	28.15695128957595	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65627.0
3	21261.0
4	11257.0
5	8017.0
6	6810.0
7	6871.0
8	6995.0
9	7751.0
10	8422.0
11	9123.0
12	9659.0
13	10577.0
14	11186.0
15	12013.0
16	12768.0
17	13436.0
18	13098.0
19	11808.0
20	11324.0
21	11438.0
22	11965.0
23	13638.0
24	15875.0
25	19135.0
26	22540.0
27	27329.0
28	33158.0
29	39970.0
30	48467.0
31	59924.0
32	75957.0
33	100925.0
34	141136.0
35	210832.0
36	370987.0
37	723794.0
38	806624.0
39	104785.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.50819056984628	19.758682262822834	13.448741007194245	26.28438616013664
2	14.879259597516919	21.011646134737596	37.64567356848652	26.463420699258965
3	17.495231186522133	24.853738084425924	29.344109699827982	28.306921029223957
4	12.644515647915522	17.17170640614852	36.15173767581923	34.03204027011673
5	13.776829510563829	37.39842782177242	35.0103449779614	13.814397689702346
6	33.275815296374525	36.361217147544586	17.02511287228477	13.337854683796127
7	28.43299963216333	31.780571700216022	21.55032015354194	18.236108514078715
8	26.355998079111043	35.24501829979367	20.01067016999609	18.388313451099197
9	26.315573609018234	15.420015359365888	19.364846945662855	38.899564085953024
10-11	25.23548186928945	25.537066675799725	28.365175452470105	20.862276002440723
12-13	25.84766549059818	22.882115954432056	27.917890504525307	23.352328050444456
14-15	22.927624859410287	24.503376171648235	25.13502668075861	27.433972288182872
16-17	23.396369913280633	27.482893896542286	25.88118938182276	23.23954680835432
18-19	23.310839723746827	26.966592226221447	26.987680994433923	22.73488705559781
20-21	23.911682886169384	26.182779273742618	26.87538874811964	23.030149091968358
22-23	23.64235489101668	26.1415128066261	26.55469124164415	23.661441060713074
24-25	23.463516024219846	26.216409723462437	26.693404648972752	23.626669603344972
26-27	23.37496288026234	26.452522180203875	26.55317136989958	23.619343569634204
28-29	23.503128153445605	26.624014897706644	26.368614567863	23.504242380984756
30-31	23.71975473193367	26.38850734320006	26.306400325973257	23.58533759889301
32-33	23.49351769311267	26.397797888970715	26.207414441300724	23.901269976615893
34-35	23.814932175947533	26.38767278185037	26.331280584127565	23.466114458074532
36-37	23.923154620080595	26.1550907964949	26.340018360342306	23.581736223082196
38-39	23.631342397164783	26.349361517566866	26.332600566513687	23.686695518754664
40-41	23.903388441789588	26.17014906766904	26.387693496177313	23.538768994364062
42-43	23.72282535411559	26.155630475361814	26.22101708885361	23.900527081668997
44-45	23.700363712936944	26.20972944621423	26.351755609710487	23.738151231138335
46-47	23.857539980186523	26.227212282740076	26.11569487247598	23.79955286459742
48-49	23.576356126029218	26.321091357086075	26.201946018990586	23.900606497894124
50-51	23.725151567264863	26.332746360891974	26.188288163906602	23.75381390793656
52-53	23.903484054655358	26.199473521204	26.13163713848989	23.765405285650758
54-55	23.510207008051083	26.14567848045749	26.363709176569984	23.980405334921436
56-57	23.565100264498614	26.228770389074086	26.27489091188316	23.93123843454414
58-59	23.552195384602715	26.116837932995296	26.354886528823503	23.97608015357849
60-61	23.548084233399454	26.214984300383627	26.419311852322192	23.817619613894724
62-63	23.570784272081905	26.141013766087152	26.38585905406704	23.902342907763906
64-65	23.33112132183087	26.114362717763058	26.315543763904497	24.238972196501578
66-67	23.33790414036918	26.179527534628832	26.23751490278593	24.245053422216063
68-69	23.542108919368648	26.07972665148064	26.220875943068545	24.157288486082166
70-71	23.411762709865165	25.92528917180162	26.218221802467202	24.444726315866014
72-73	23.39278930147933	26.023500253274477	26.15711546214839	24.426594983097804
74-75	23.36547787582747	26.13314963485081	26.06100006379915	24.440372425522565
76-77	23.4524299843587	25.861238308474217	26.205945634961413	24.480386072205665
78-79	23.358203564189	25.88721304247979	26.220922443310695	24.533660950020515
80-81	23.454961274054053	25.987021225508872	26.076520543155347	24.481496957281728
82-83	23.47435508757739	25.940909994674755	26.15232068008051	24.432414237667345
84-85	23.38452386953021	25.9493173948951	26.088707644944538	24.577451090630156
86-87	23.358469774062876	25.986372354331717	26.063074315426515	24.592083556178896
88-89	23.23756991970772	25.874939675527216	26.08357328168371	24.803917123081355
90-91	23.195408214742823	25.887531967248822	26.14424166827134	24.772818149737013
92-93	23.294543022348453	25.690785806720527	26.23330085997845	24.781370310952568
94-95	23.1245164406544	25.62853631158251	26.25900645682432	24.987940790938772
96-97	23.092563534919417	25.502339459705286	26.299525777338218	25.105571228037082
98-99	22.968572125220614	25.489131926670982	26.208745161806	25.3335507863024
100-101	22.90012301068407	25.464438802911303	26.28294911736847	25.352489069036153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	18.0
2	28.5
3	44.0
4	45.5
5	53.0
6	100.0
7	205.5
8	297.5
9	317.0
10	274.0
11	236.0
12	272.0
13	303.0
14	326.0
15	366.5
16	400.5
17	464.0
18	533.0
19	606.0
20	721.5
21	892.5
22	1079.5
23	1370.0
24	1806.5
25	2304.0
26	3104.5
27	4425.5
28	6306.0
29	9293.5
30	13675.0
31	19444.5
32	27358.5
33	34406.5
34	40525.5
35	48316.5
36	57885.0
37	67797.5
38	76541.5
39	84631.0
40	92513.0
41	100561.5
42	107192.0
43	112807.5
44	118858.0
45	124887.0
46	130018.5
47	133763.5
48	135853.0
49	136178.0
50	135014.5
51	133107.0
52	130975.5
53	128524.0
54	124211.5
55	116776.0
56	107949.5
57	97331.0
58	86749.0
59	74779.5
60	60576.5
61	49291.0
62	40019.5
63	30127.0
64	21253.0
65	15417.5
66	11651.0
67	9052.0
68	6763.5
69	4846.0
70	3581.0
71	2648.5
72	1883.5
73	1356.0
74	1056.5
75	796.5
76	540.5
77	318.5
78	185.0
79	106.5
80	55.5
81	35.5
82	25.0
83	16.5
84	12.0
85	8.5
86	6.0
87	4.0
88	5.0
89	4.0
90	2.0
91	2.5
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16544576540546163
2	1.6147351688996839E-4
3	0.010495778597847946
4	0.0062005830485747865
5	0.02512527922807908
6	0.0020345663128136016
7	0.0
8	0.0
9	3.229470337799368E-5
10-11	0.048425907715301524
12-13	2.5835762702394944E-4
14-15	0.004860352858388048
16-17	1.614735168899684E-5
18-19	0.0015501457621436966
20-21	1.6147351688996839E-4
22-23	0.0
24-25	4.521258472919115E-4
26-27	0.002728902435440466
28-29	0.005409362815813942
30-31	0.01690627721837969
32-33	0.011722977326211706
34-35	0.01001135804717804
36-37	0.004375932307718143
38-39	0.0
40-41	0.003358649151311343
42-43	0.009155548407661208
44-45	0.007185571501603593
46-47	0.003407091206378333
48-49	0.008541949043479328
50-51	0.0027611971388184593
52-53	0.002002271609435608
54-55	0.004198311439139178
56-57	0.007992939086053436
58-59	0.015388426159613987
60-61	0.007395487073560553
62-63	0.02100770454738489
64-65	0.04072362095965003
66-67	0.05953528567733135
68-69	0.049507780278464315
70-71	0.06735060389480581
72-73	0.03316666036919951
74-75	0.02682075115542375
76-77	0.22301107417673532
78-79	0.08472515431216641
80-81	0.06607496311137506
82-83	0.02743435051960563
84-85	0.05086415782034004
86-87	0.06581660548435112
88-89	0.291072161545857
90-91	0.29528662033668523
92-93	0.05289872413315364
94-95	0.009736853068465095
96-97	0.015146215884279035
98-99	9.04251694583823E-4
100-101	1.2917881351197472E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3096483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.28386830857785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58727317561546	48.32435504891688
2	12.853394701955509	16.011182858695385
3	4.1895985501947095	7.828332130984079
4	1.8720684317503153	4.6639865467113
5	1.0039601992486653	3.126526241852872
6	0.6253667188660295	2.337015501745152
7	0.3879347470277085	1.6913453687336681
8	0.2776643917067932	1.3835209925637824
9	0.20590049982269404	1.154185165406433
>10	0.9485496161474495	9.990719176465698
>50	0.0365509310475674	1.5115074254697125
>100	0.010179458644772602	1.0882527061171408
>500	9.870993829718731E-4	0.4381608749878913
>1k	5.714785893495798E-4	0.4509099613500733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.229470337799368E-5	9.688411013398104E-5
2	0.0	0.0	0.0	3.229470337799368E-5	9.688411013398104E-5
3	0.0	0.0	0.0	3.229470337799368E-5	9.688411013398104E-5
4	0.0	0.0	0.0	3.229470337799368E-5	9.688411013398104E-5
5	0.0	3.229470337799368E-5	0.0	3.229470337799368E-5	9.688411013398104E-5
6	0.0	3.229470337799368E-5	0.0	3.229470337799368E-5	1.9376822026796208E-4
7	0.0	3.229470337799368E-5	0.0	3.229470337799368E-5	1.9376822026796208E-4
8	0.0	3.229470337799368E-5	0.0	3.229470337799368E-5	2.2606292364595574E-4
9	0.0	3.229470337799368E-5	0.0	3.229470337799368E-5	2.2606292364595574E-4
10-11	0.0	3.229470337799368E-5	0.0	6.458940675598736E-5	2.2606292364595574E-4
12-13	0.0	4.844205506699052E-5	0.0	6.458940675598736E-5	2.9065233040194313E-4
14-15	0.0	6.458940675598736E-5	0.0	6.458940675598736E-5	3.5524173715793046E-4
16-17	0.0	6.458940675598736E-5	0.0	6.458940675598736E-5	3.8753644053592416E-4
18-19	0.0	6.458940675598736E-5	0.0	6.458940675598736E-5	4.198311439139178E-4
20-21	0.0	6.458940675598736E-5	0.0	9.688411013398104E-5	4.198311439139178E-4
22-23	0.0	6.458940675598736E-5	0.0	1.2917881351197472E-4	4.844205506699052E-4
24-25	0.0	8.07367584449842E-5	0.0	2.5835762702394944E-4	5.167152540478989E-4
26-27	0.0	9.688411013398104E-5	0.0	5.490099574258925E-4	5.813046608038863E-4
28-29	0.0	9.688411013398104E-5	0.0	0.0022121871813925668	5.813046608038863E-4
30-31	0.0	9.688411013398104E-5	0.0	0.011060935906962836	5.813046608038863E-4
32-33	0.0	9.688411013398104E-5	0.0	0.031180536111452897	6.458940675598735E-4
34-35	0.0	9.688411013398104E-5	0.0	0.05880865485132648	6.781887709378673E-4
36-37	0.0	9.688411013398104E-5	0.0	0.10240650441161796	6.781887709378673E-4
38-39	0.0	9.688411013398104E-5	0.0	0.1772979215451853	6.781887709378673E-4
40-41	0.0	9.688411013398104E-5	0.0	0.2610703821077009	6.781887709378673E-4
42-43	0.0	1.2917881351197472E-4	0.0	0.35143096215932723	6.943361226268641E-4
44-45	0.0	1.2917881351197472E-4	0.0	0.4594083029036491	7.266308260048577E-4
46-47	0.0	1.614735168899684E-4	0.0	0.5824834174771829	8.07367584449842E-4
48-49	0.0	1.9376822026796208E-4	0.0	0.7197359068336562	9.04251694583823E-4
50-51	0.0	1.9376822026796208E-4	0.0	0.8657241134538766	9.365463979618167E-4
52-53	0.0	1.9376822026796208E-4	0.0	1.0177675769574708	0.0010657252114737914
54-55	0.0	1.9376822026796208E-4	0.0	1.185538561006148	0.0011787566732967695
56-57	0.0	1.9376822026796208E-4	0.0	1.3806470114642968	0.0012110513766747629
58-59	0.0	1.9376822026796208E-4	0.0	1.5905948781246337	0.0012271987283637598
60-61	0.0	2.422102753349526E-4	0.0	1.8113937651199765	0.0012271987283637598
62-63	0.0	2.5835762702394944E-4	0.0	2.038344793108827	0.0012271987283637598
64-65	0.0	2.5835762702394944E-4	0.0	2.2832516761758423	0.001307935486808744
66-67	0.0	2.5835762702394944E-4	0.0	2.5601949049938266	0.0013240828384977407
68-69	0.0	2.5835762702394944E-4	0.0	2.8673982708769916	0.0013402301901867376
70-71	0.0	2.5835762702394944E-4	0.0	3.19289658622379	0.0013563775418757346
72-73	0.0	2.5835762702394944E-4	0.0	3.532443097540016	0.0016308825205886808
74-75	0.0	2.9065233040194313E-4	0.0	3.8854403528131756	0.0016631772239666743
76-77	0.0	3.2294703377993677E-4	0.0	4.274946770255157	0.0016793245756556712
78-79	0.0	3.2294703377993677E-4	0.0	4.713557284183379	0.0016793245756556712
80-81	0.0	3.3909438546893364E-4	0.0	5.175533016005578	0.0017923560374786492
82-83	0.0	3.5524173715793046E-4	0.0	5.66082552366669	0.0020022716094356082
84-85	0.0	3.8753644053592416E-4	0.0	6.158196250391169	0.0020022716094356082
86-87	0.0	3.8753644053592416E-4	0.0	6.686844397337238	0.002050713664502599
88-89	0.0	3.8753644053592416E-4	0.0	7.260010792889869	0.002099155719569589
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2150	0.0	42.45195	1
GTATCAA	3445	0.0	40.016922	1
TCAACGC	4060	0.0	33.201153	4
CAACGCA	4235	0.0	31.84927	5
ATCAACG	4300	0.0	31.458956	3
AACGCAG	4575	0.0	29.476612	6
TATCAAC	4670	0.0	29.270926	2
GTACATG	7015	0.0	25.954102	1
TACATGG	7440	0.0	24.050787	2
ACGCAGA	5680	0.0	23.32417	7
CGCAGAG	5935	0.0	22.322037	8
ACATGGG	7680	0.0	21.93992	3
CATGGGG	6080	0.0	19.047901	4
GGACCGT	445	0.0	18.140083	6
GCAGAGT	7445	0.0	16.455282	9
AGAGTAC	5965	0.0	16.438416	10-11
GTATTAA	2015	0.0	16.042377	1
GAGTACA	5265	0.0	15.529767	1
TATACTG	1390	0.0	15.3756075	5
GTATTAG	930	0.0	15.334626	1
>>END_MODULE