FastQCFastQC Report
Mon 15 Aug 2016
SRR2921696_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921696_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268147
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27100.042.737871
GGTATCA15600.040.7729841
TCAACGC30700.036.9523664
CAACGCA31000.036.1314245
ATCAACG33600.033.4819683
AACGCAG33450.033.3247646
TATCAAC34700.032.4205782
ACGCAGA41400.026.8150047
CGCAGAG42900.025.3261578
GTACATG54950.023.4095951
TACATGG56750.022.2493672
ACATGGG56700.021.4318073
GCAGAGT52600.019.0342469
CATGGGG47500.018.9864044
AGAGTAC43700.018.24196610-11
GAGTACA43100.018.061711
GTATTAG6150.017.7519071
AGTACAT42900.017.2611272
TATACAG14400.016.4794355
ACCGTGC5500.016.3901168
Produced by FastQC (version 0.11.5)