##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921696_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2268147
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.058511639677675	31.0	31.0	34.0	26.0	34.0
2	30.115108500463155	31.0	31.0	34.0	26.0	34.0
3	30.177187810137525	31.0	31.0	34.0	26.0	34.0
4	33.60155272123015	37.0	35.0	37.0	28.0	37.0
5	33.5959014120337	37.0	35.0	37.0	28.0	37.0
6	33.632120404894394	37.0	35.0	37.0	28.0	37.0
7	33.54198956240491	37.0	35.0	37.0	28.0	37.0
8	33.51076363216317	37.0	35.0	37.0	28.0	37.0
9	35.04183855808287	39.0	35.0	39.0	28.0	39.0
10-11	34.935445321665654	39.0	35.0	39.0	27.0	39.0
12-13	34.82658222769512	39.0	35.0	39.0	27.0	39.0
14-15	35.948144454482005	40.0	36.0	41.0	27.0	41.0
16-17	35.89243466142186	40.0	36.0	41.0	27.0	41.0
18-19	35.855691892985774	40.0	36.0	41.0	27.0	41.0
20-21	35.782698608158995	40.0	36.0	41.0	27.0	41.0
22-23	35.68644250130173	40.0	36.0	41.0	26.5	41.0
24-25	35.58181039412348	40.0	36.0	41.0	25.5	41.0
26-27	35.442780825052346	40.0	36.0	41.0	25.0	41.0
28-29	35.30507943268228	40.0	36.0	41.0	24.0	41.0
30-31	35.159582690187186	40.0	35.5	41.0	24.0	41.0
32-33	34.991596664590084	39.0	35.0	41.0	23.5	41.0
34-35	34.8278369964557	39.0	35.0	41.0	23.0	41.0
36-37	34.798162993844755	39.0	35.0	41.0	22.5	41.0
38-39	34.976463827079996	39.5	35.0	41.0	23.0	41.0
40-41	35.04292667097856	40.0	35.0	41.0	22.5	41.0
42-43	34.890292824935955	40.0	35.0	41.0	21.0	41.0
44-45	34.78070623288526	40.0	35.0	41.0	19.5	41.0
46-47	34.62784136125216	40.0	35.0	41.0	18.5	41.0
48-49	34.47186800502789	39.0	35.0	41.0	15.0	41.0
50-51	32.82323830862814	37.0	32.5	39.5	13.5	40.0
52-53	32.94177846497604	37.5	32.5	39.5	12.5	40.0
54-55	33.85214781052551	39.0	34.0	40.5	12.5	41.0
56-57	33.87827728978766	39.0	34.0	41.0	9.5	41.0
58-59	33.7027251761019	38.5	34.0	41.0	8.0	41.0
60-61	33.465967373366894	38.0	34.0	40.5	7.0	41.0
62-63	33.19229992588664	37.5	33.0	40.0	4.0	41.0
64-65	32.91348113680462	37.0	33.0	40.0	2.0	41.0
66-67	32.58291415856203	36.5	33.0	39.5	2.0	41.0
68-69	32.23665243037598	36.0	32.5	39.0	2.0	41.0
70-71	31.84478188583015	35.5	32.0	39.0	2.0	41.0
72-73	31.49383571699718	35.0	32.0	38.5	2.0	40.0
74-75	31.100723409902443	35.0	32.0	37.0	2.0	39.5
76-77	30.729021752117475	35.0	31.0	37.0	2.0	39.0
78-79	30.390401063070428	35.0	31.0	36.0	2.0	39.0
80-81	30.077657224156987	35.0	31.0	36.0	2.0	37.0
82-83	29.770635677493566	35.0	31.0	35.5	2.0	37.0
84-85	29.5132804443451	35.0	31.0	35.0	2.0	36.5
86-87	29.286952300710666	35.0	30.5	35.0	2.0	36.0
88-89	29.076396944289765	35.0	30.0	35.0	2.0	36.0
90-91	28.867818532043998	34.0	30.0	35.0	2.0	36.0
92-93	28.699909882384166	34.0	30.0	35.0	2.0	35.0
94-95	28.48914466302228	34.0	30.0	35.0	2.0	35.0
96-97	28.279389739730274	34.0	30.0	35.0	2.0	35.0
98-99	28.039123786950316	34.0	29.0	35.0	2.0	35.0
100-101	27.00873069514454	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97738.0
3	15563.0
4	10015.0
5	7557.0
6	6522.0
7	6794.0
8	7094.0
9	7511.0
10	8275.0
11	8623.0
12	8572.0
13	8542.0
14	9210.0
15	9768.0
16	9811.0
17	10297.0
18	9665.0
19	9114.0
20	9255.0
21	9584.0
22	10506.0
23	12175.0
24	14038.0
25	16526.0
26	19645.0
27	23326.0
28	27478.0
29	32797.0
30	39136.0
31	47493.0
32	59140.0
33	76570.0
34	105515.0
35	152328.0
36	256121.0
37	472818.0
38	531781.0
39	101224.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01966155586199	17.943529710687695	13.222497774554668	24.81431095889565
2	15.586069846665247	19.1744110578567	40.1555011098713	25.08401798560675
3	19.305557087487013	23.560189096716837	28.867383022326738	28.266870793469412
4	13.045935528958319	15.810644110390687	37.0698783358805	34.07354202477049
5	14.438317945025625	36.92695318566205	34.42308846159276	14.211640407719567
6	33.542732623254125	35.4798854984047	17.227401720345902	13.749980157995271
7	29.151321777044043	31.281924425536715	20.985459394247254	18.581294403171984
8	26.389655677242175	35.24422380538294	19.698724201369107	18.667396316005778
9	26.27202044439036	16.08840540986223	19.346906489665372	38.29266765608204
10-11	25.513103765454403	25.38002562349513	27.94325703240594	21.16361357864453
12-13	26.200598466372043	22.595666531944484	27.700733298803883	23.50300170287959
14-15	23.20389304448658	24.251650587524914	24.944568752169506	27.599887615819004
16-17	23.605579490182883	27.343409250374528	25.625430261003324	23.425580998439262
18-19	23.59159315672428	26.721043189695447	26.794172822717854	22.893190830862427
20-21	24.18557445697614	25.917593020507372	26.78493626944285	23.111896253073635
22-23	23.87286143369397	25.832601916825816	26.4387881567863	23.85574849269392
24-25	23.679545900601806	25.98761988140314	26.605457043370105	23.727377174624948
26-27	23.62297174834796	26.176865528486477	26.424345855079366	23.775816868086196
28-29	23.780512809658802	26.23295804545168	26.308998928063872	23.677530216825648
30-31	23.92972315965782	26.036599709990853	26.26924198787447	23.76443514247686
32-33	23.63652557829755	26.15529986946237	26.231713213181102	23.976461339058968
34-35	24.00683592910369	26.124942212721997	26.247097408682873	23.62112444949144
36-37	24.04982172515031	25.988139112139663	26.212553353493874	23.74948580921615
38-39	23.856634711938987	26.084228649910003	26.254837575408857	23.804299062742153
40-41	24.108616899866565	25.924108462586943	26.269571012272948	23.697703625273547
42-43	23.882975303462047	25.93495520014388	26.26639437439392	23.915675122000152
44-45	23.929300561846766	26.064437553549052	26.219714621511596	23.786547263092583
46-47	24.115987385338073	26.048698143828087	25.98181224988199	23.853502220951853
48-49	23.876743673337025	26.087314775092484	26.080532933462404	23.95540861810809
50-51	23.912665568856653	26.175523696240166	26.11349853496481	23.798312199938373
52-53	24.16476173729559	26.03076044898309	26.01777129007089	23.78670652365043
54-55	23.730173754593807	25.952166735843935	26.37750200628803	23.940157503274232
56-57	23.848476206528407	26.083055199228255	26.2348149161025	23.833653678140834
58-59	23.930412260103694	25.970449048014498	26.24814784622159	23.85099084566022
60-61	23.85899252919337	25.965996396603398	26.35488043726809	23.820130636935147
62-63	23.896845884248506	25.93558080960924	26.409849051485608	23.757724254656644
64-65	23.76765922516553	25.798318251080087	26.382773052255448	24.051249471498938
66-67	23.730176348902887	25.999728303187137	26.266322517370373	24.003772830539603
68-69	23.84066918324568	25.897585983729854	26.288281562340547	23.97346327068392
70-71	23.805784904015987	25.678288731030303	26.301411252250322	24.214515112703392
72-73	23.703616166390923	25.871085026628116	26.28542776171427	24.139871045266688
74-75	23.75077116981242	25.931494718715463	26.260372551501334	24.05736155997078
76-77	23.790598137686125	25.698787362346344	26.34128520772897	24.16932929223856
78-79	23.7224519732075	25.71748581729495	26.38506706961455	24.174995139883006
80-81	23.795002772800483	25.839738760215724	26.23144377843225	24.13381468855154
82-83	23.856264601171233	25.873463217752374	26.25970520823529	24.010566972841104
84-85	23.80297923303097	25.82645582084217	26.34085258988964	24.029712356237216
86-87	23.758621322079314	25.88561000169482	26.28271064331806	24.073058032907802
88-89	23.776367471672415	25.737617515586443	26.289442803391776	24.19657220934937
90-91	23.69367312986582	25.77889066374166	26.349382210554207	24.178053995838315
92-93	23.747934887731027	25.672773086999534	26.555603748566824	24.023688276702618
94-95	23.687656386582773	25.59253445476489	26.535140634128535	24.184668524523804
96-97	23.726368177893786	25.47192930777956	26.572053564542692	24.22964894978396
98-99	23.620426556432488	25.470088416263074	26.54297581909998	24.366509208204455
100-101	23.81550917946134	25.312044231126198	26.626802082504664	24.245644506907798
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	11.0
2	23.5
3	33.5
4	25.0
5	48.5
6	84.0
7	160.5
8	263.5
9	295.5
10	283.0
11	282.5
12	309.0
13	371.5
14	463.5
15	544.5
16	611.0
17	677.0
18	722.5
19	779.0
20	868.0
21	988.0
22	1088.0
23	1277.5
24	1551.5
25	1858.0
26	2338.5
27	3181.0
28	4529.5
29	6509.5
30	9457.0
31	13558.5
32	19162.0
33	24434.5
34	29044.0
35	34456.5
36	40891.5
37	47812.5
38	54442.5
39	60424.0
40	65795.5
41	70911.0
42	75953.5
43	81160.0
44	86090.0
45	91101.5
46	94549.0
47	96835.5
48	99019.5
49	99435.0
50	98003.5
51	96588.5
52	95483.0
53	93997.0
54	92038.5
55	87089.5
56	79994.5
57	71888.0
58	64592.0
59	56903.5
60	47060.5
61	38669.0
62	31293.0
63	23740.0
64	17042.0
65	12462.5
66	9468.5
67	6967.0
68	5152.5
69	3941.0
70	2928.5
71	2118.5
72	1515.5
73	1142.0
74	887.0
75	634.5
76	443.5
77	314.0
78	223.5
79	159.0
80	112.0
81	92.0
82	75.0
83	54.5
84	44.5
85	46.5
86	44.5
87	36.0
88	28.5
89	23.0
90	24.5
91	22.0
92	17.5
93	14.5
94	10.0
95	12.0
96	10.5
97	7.5
98	6.0
99	3.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05224529097981745
2	0.07354020705007215
3	0.06974856567938498
4	0.06961629911994241
5	0.06573648004296018
6	0.010184525077078337
7	0.012829856265929855
8	0.017238741580682383
9	0.006216528293801062
10-11	0.013513233489716496
12-13	0.022815981503844326
14-15	0.040738100308313346
16-17	0.027687799776645868
18-19	0.011308790832340232
20-21	0.020942205245074504
22-23	0.037784147147429156
24-25	0.035359260224315264
26-27	0.021691715748582432
28-29	0.012697589706487277
30-31	0.012675545279913514
32-33	0.009633414412734272
34-35	0.005753595335752048
36-37	8.817770629505054E-4
38-39	0.004166396622441138
40-41	0.0012565323147044703
42-43	0.22650648304541107
44-45	0.19626152978620875
46-47	0.20238988037371478
48-49	0.20942205245074505
50-51	0.2005822373946662
52-53	0.20814347570946679
54-55	0.20450614532479594
56-57	0.20723965421994253
58-59	0.21618969140889016
60-61	0.22064266557679021
62-63	0.20545405566746777
64-65	0.20589494419894303
66-67	0.20256623578630484
68-69	0.1980471283386835
70-71	0.18986864607981757
72-73	0.2011333480590103
74-75	0.2001854377163385
76-77	0.19416730926170128
78-79	0.21281689414310448
80-81	0.22450044022719867
82-83	0.2230014192201828
84-85	0.21663057994036541
86-87	0.23677918582878446
88-89	0.22937225850000023
90-91	0.23638238615045676
92-93	0.23170896771681904
94-95	0.23477314301057206
96-97	0.2422021147659301
98-99	0.23490540957001463
100-101	0.24191553722047116
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2268147.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.39048239468096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.01511764805113	57.604590596494596
2	10.230879188463799	14.1985128441841
3	2.990246384006469	6.224839171954777
4	1.2863873818840963	3.570521639014724
5	0.6921608373647457	2.4014687199723004
6	0.41684234105818957	1.735493467713354
7	0.2879736973798657	1.3987843644718125
8	0.20413307940465308	1.133191428208047
9	0.1508573147551332	0.9421255659262422
>10	0.6921973537426922	8.258585272798543
>50	0.02484212480216365	1.1591474914731041
>100	0.007342952738645482	0.889732954406875
>500	9.559653267007098E-4	0.43781030813738436
>1k	6.37310217799529E-5	0.045196175244028206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.408885314752527E-5	0.0
6	0.0	0.0	0.0	4.408885314752527E-5	0.0
7	0.0	0.0	0.0	4.408885314752527E-5	0.0
8	0.0	0.0	0.0	4.408885314752527E-5	0.0
9	0.0	0.0	0.0	8.817770629505054E-5	0.0
10-11	0.0	0.0	0.0	1.322665594425758E-4	0.0
12-13	0.0	0.0	0.0	1.5431098601633846E-4	8.817770629505054E-5
14-15	0.0	2.2044426573762635E-5	0.0	1.7635541259010108E-4	1.322665594425758E-4
16-17	0.0	4.408885314752527E-5	0.0	1.7635541259010108E-4	2.2044426573762635E-4
18-19	0.0	4.408885314752527E-5	0.0	1.7635541259010108E-4	2.645331188851516E-4
20-21	0.0	4.408885314752527E-5	0.0	1.7635541259010108E-4	2.645331188851516E-4
22-23	0.0	4.408885314752527E-5	0.0	1.7635541259010108E-4	3.306663986064395E-4
24-25	0.0	4.408885314752527E-5	0.0	2.2044426573762635E-4	3.967996783277274E-4
26-27	0.0	4.408885314752527E-5	0.0	3.747552517539648E-4	4.188441049014901E-4
28-29	4.408885314752527E-5	4.408885314752527E-5	0.0	0.0011903990349831823	4.408885314752527E-4
30-31	8.817770629505054E-5	4.408885314752527E-5	0.0	0.005378840083998083	4.408885314752527E-4
32-33	8.817770629505054E-5	4.408885314752527E-5	0.0	0.015871987133109097	4.408885314752527E-4
34-35	8.817770629505054E-5	8.817770629505054E-5	0.0	0.029120687503940437	4.8497738462277795E-4
36-37	8.817770629505054E-5	1.1022213286881318E-4	0.0	0.05213506884694863	4.8497738462277795E-4
38-39	8.817770629505054E-5	1.5431098601633846E-4	0.0	0.09547441149096597	4.8497738462277795E-4
40-41	8.817770629505054E-5	2.2044426573762635E-4	0.0	0.14615454818404627	4.8497738462277795E-4
42-43	8.817770629505054E-5	2.645331188851516E-4	0.0	0.19718739570230676	4.8497738462277795E-4
44-45	8.817770629505054E-5	2.645331188851516E-4	0.0	0.26093987735362834	5.070218111965406E-4
46-47	8.817770629505054E-5	2.645331188851516E-4	0.0	0.3376985706834698	5.290662377703032E-4
48-49	8.817770629505054E-5	2.645331188851516E-4	0.0	0.42345139005540644	5.290662377703032E-4
50-51	8.817770629505054E-5	2.645331188851516E-4	0.0	0.5185730907211922	5.290662377703032E-4
52-53	8.817770629505054E-5	2.645331188851516E-4	0.0	0.6111596823309953	5.731550909178285E-4
54-55	8.817770629505054E-5	2.645331188851516E-4	0.0	0.7171051964444985	6.172439440653537E-4
56-57	8.817770629505054E-5	2.645331188851516E-4	0.0	0.8419868729848639	6.172439440653537E-4
58-59	8.817770629505054E-5	2.645331188851516E-4	0.0	0.9802495164555031	6.172439440653537E-4
60-61	8.817770629505054E-5	2.645331188851516E-4	0.0	1.1343180137795301	6.172439440653537E-4
62-63	8.817770629505054E-5	2.645331188851516E-4	0.0	1.2935449069218177	7.495105035079296E-4
64-65	8.817770629505054E-5	2.645331188851516E-4	0.0	1.4572027298054315	7.495105035079296E-4
66-67	8.817770629505054E-5	2.645331188851516E-4	0.0	1.6434781343537257	7.495105035079296E-4
68-69	8.817770629505054E-5	2.645331188851516E-4	0.0	1.8558100511122073	7.495105035079296E-4
70-71	8.817770629505054E-5	2.645331188851516E-4	0.0	2.085689331423404	7.495105035079296E-4
72-73	8.817770629505054E-5	2.645331188851516E-4	0.0	2.321322207070353	8.376882098029802E-4
74-75	8.817770629505054E-5	2.645331188851516E-4	0.0	2.567205741074101	8.376882098029802E-4
76-77	8.817770629505054E-5	2.645331188851516E-4	0.0	2.835199834931334	8.376882098029802E-4
78-79	8.817770629505054E-5	2.645331188851516E-4	0.0	3.140801720523405	8.376882098029802E-4
80-81	8.817770629505054E-5	2.645331188851516E-4	0.0	3.467257633654256	8.817770629505054E-4
82-83	8.817770629505054E-5	2.645331188851516E-4	0.0	3.8161547730371974	9.699547692455559E-4
84-85	8.817770629505054E-5	2.645331188851516E-4	0.0	4.175853681441282	9.699547692455559E-4
86-87	8.817770629505054E-5	2.645331188851516E-4	0.0	4.551292310419034	9.699547692455559E-4
88-89	8.817770629505054E-5	2.645331188851516E-4	0.0	4.971944058299572	9.699547692455559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2710	0.0	42.73787	1
GGTATCA	1560	0.0	40.772984	1
TCAACGC	3070	0.0	36.952366	4
CAACGCA	3100	0.0	36.131424	5
ATCAACG	3360	0.0	33.481968	3
AACGCAG	3345	0.0	33.324764	6
TATCAAC	3470	0.0	32.420578	2
ACGCAGA	4140	0.0	26.815004	7
CGCAGAG	4290	0.0	25.326157	8
GTACATG	5495	0.0	23.409595	1
TACATGG	5675	0.0	22.249367	2
ACATGGG	5670	0.0	21.431807	3
GCAGAGT	5260	0.0	19.034246	9
CATGGGG	4750	0.0	18.986404	4
AGAGTAC	4370	0.0	18.241966	10-11
GAGTACA	4310	0.0	18.06171	1
GTATTAG	615	0.0	17.751907	1
AGTACAT	4290	0.0	17.261127	2
TATACAG	1440	0.0	16.479435	5
ACCGTGC	550	0.0	16.390116	8
>>END_MODULE