FastQCFastQC Report
Mon 15 Aug 2016
SRR2921696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268147
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.045.2562521
GTATCAA30650.040.0110051
TCAACGC36550.032.9818654
CAACGCA36800.032.6495
ATCAACG37750.031.9334373
TATCAAC40200.030.2233682
AACGCAG40050.029.9949466
ACGCAGA49650.024.3855117
CGCAGAG50050.023.5265648
GTACATG55800.023.5106771
TACATGG59200.021.5654982
ACATGGG61100.019.3413643
GTATATA10200.019.1061841
GCAGAGT61950.018.2409119
CATGGGG52800.017.9773224
AGAGTAC50450.017.74030510-11
TCTATAC7000.017.6280483
GTATAGG6600.016.564341
GTATAGA7850.016.3487421
GTATAGT6400.016.3392811
Produced by FastQC (version 0.11.5)