##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921696_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2268147
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259534765603817	33.0	31.0	34.0	28.0	34.0
2	31.39637289822926	34.0	31.0	34.0	28.0	34.0
3	31.472979485015742	34.0	31.0	34.0	28.0	34.0
4	34.932652954151564	37.0	35.0	37.0	32.0	37.0
5	34.668605694428095	37.0	35.0	37.0	32.0	37.0
6	34.64007315222514	37.0	35.0	37.0	32.0	37.0
7	34.529806048726115	37.0	35.0	37.0	32.0	37.0
8	34.476387112475514	37.0	35.0	37.0	31.0	37.0
9	36.061349639154784	39.0	37.0	39.0	31.0	39.0
10-11	35.948039963900044	39.0	37.0	39.0	31.0	39.0
12-13	35.8659044144846	39.0	37.0	39.0	30.0	39.0
14-15	37.113849102372996	40.0	38.0	41.0	31.0	41.0
16-17	37.080709054571855	40.0	38.0	41.0	31.0	41.0
18-19	37.03967181139494	40.0	38.0	41.0	31.0	41.0
20-21	36.98353678134618	40.0	38.0	41.0	31.0	41.0
22-23	36.89267737055843	40.0	37.5	41.0	30.0	41.0
24-25	36.814950706457736	40.0	37.0	41.0	30.0	41.0
26-27	36.6920891811686	40.0	37.0	41.0	30.0	41.0
28-29	36.580459732107315	40.0	37.0	41.0	30.0	41.0
30-31	36.426766430923564	40.0	37.0	41.0	30.0	41.0
32-33	36.37471446956481	40.0	37.0	41.0	29.5	41.0
34-35	36.51015652865533	40.0	37.0	41.0	30.0	41.0
36-37	36.51075481439254	40.0	37.0	41.0	30.0	41.0
38-39	36.407474471451806	40.0	37.0	41.0	29.5	41.0
40-41	36.328841560974666	40.0	37.0	41.0	29.0	41.0
42-43	36.21201381568302	40.0	37.0	41.0	29.0	41.0
44-45	36.087492345072874	40.0	36.0	41.0	28.0	41.0
46-47	35.93790371611716	40.0	36.0	41.0	28.0	41.0
48-49	35.8022334972116	40.0	36.0	41.0	27.0	41.0
50-51	35.651691005918046	40.0	35.5	41.0	27.0	41.0
52-53	35.486490954951336	39.5	35.0	41.0	26.5	41.0
54-55	35.283780769059504	39.0	35.0	41.0	26.0	41.0
56-57	35.06748085551774	39.0	35.0	41.0	26.0	41.0
58-59	34.833286158260464	39.0	35.0	41.0	26.0	41.0
60-61	34.56630544669283	38.0	34.5	40.0	25.0	41.0
62-63	34.270275030674824	38.0	34.0	40.0	24.0	41.0
64-65	33.94125513028918	37.0	34.0	40.0	23.0	41.0
66-67	33.598827589217095	36.5	34.0	39.5	22.5	41.0
68-69	33.24408029991001	36.0	34.0	39.0	22.0	41.0
70-71	32.84613827939724	35.5	33.0	39.0	21.5	40.0
72-73	32.48462665779599	35.0	33.0	38.0	20.0	40.0
74-75	32.08285441816602	35.0	33.0	37.0	19.5	39.0
76-77	29.392462437399338	33.0	29.0	34.5	13.0	36.5
78-79	30.94269044290339	34.5	31.5	36.0	14.5	38.0
80-81	31.10801239954906	35.0	32.0	36.0	17.0	37.0
82-83	30.917105240533353	35.0	32.0	35.0	13.0	37.0
84-85	30.70810357529737	35.0	32.0	35.0	10.5	36.5
86-87	30.489874113097606	35.0	32.0	35.0	7.0	36.0
88-89	30.216705971879247	35.0	32.0	35.0	2.0	36.0
90-91	30.017372551249984	34.5	31.0	35.0	2.0	35.5
92-93	29.85837161348008	34.0	31.0	35.0	2.0	35.0
94-95	29.752655140958677	34.0	31.0	35.0	2.0	35.0
96-97	29.547906066053038	34.0	31.0	35.0	2.0	35.0
98-99	29.348180695519297	34.0	31.0	35.0	2.0	35.0
100-101	28.24640378247089	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50504.0
3	16042.0
4	8377.0
5	5793.0
6	5018.0
7	4860.0
8	5117.0
9	5473.0
10	6176.0
11	6603.0
12	6868.0
13	7476.0
14	7976.0
15	8612.0
16	9331.0
17	9866.0
18	9171.0
19	8231.0
20	8129.0
21	7995.0
22	8552.0
23	9601.0
24	11220.0
25	13524.0
26	16078.0
27	19350.0
28	23394.0
29	28297.0
30	34481.0
31	42837.0
32	54576.0
33	71501.0
34	101398.0
35	153246.0
36	270911.0
37	538056.0
38	594696.0
39	78808.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.4997063408301	19.59470618627265	13.90716837489457	25.998419098002678
2	14.639760685494094	20.603665109593962	38.35023774935024	26.406336455561704
3	17.737141405843857	24.77822179716295	28.749086717823698	28.735550079169492
4	12.670056141950555	17.137514489632057	35.880539735211755	34.31188963320563
5	14.119340983057127	37.21681763258394	34.540972473342094	14.122868911016836
6	33.49944953707766	36.1377475595115	16.8559374381363	13.506865465274542
7	28.8853852946921	31.461629250661442	21.026855843117755	18.626129611528704
8	26.285289269169944	35.84952827131575	19.340413121371764	18.524769338142548
9	26.002647095910053	16.0163410997352	19.23615146467644	38.74486033967831
10-11	25.463084367843823	25.66863743240403	27.85491041349028	21.013367786261867
12-13	26.07294964155652	22.755672048462618	27.753158976077312	23.41821933390355
14-15	22.99409997308249	24.447623400023062	24.909894686624863	27.648381940269584
16-17	23.505778837478093	27.574299102813686	25.57319379502162	23.346728264686607
18-19	23.470881405790916	26.921186153721344	26.765461372141665	22.842471068346075
20-21	24.027397755519715	26.049096573318604	26.77420549507041	23.149300176091266
22-23	23.716275885116794	25.923496140241355	26.500068117278115	23.86015985736374
24-25	23.583093010223855	26.196063977017157	26.52970931521912	23.691133697539865
26-27	23.51612376988466	26.2985653063384	26.502151599590846	23.683159324186096
28-29	23.638154555960362	26.413807364792085	26.32575649016956	23.622281589077986
30-31	23.802936614415845	26.22505371519536	26.276977485368192	23.695032185020597
32-33	23.554357464525037	26.255882730336477	26.20819474863385	23.981565056504635
34-35	23.84692344629044	26.319198592890107	26.28906072592796	23.544817234891493
36-37	23.90772624391246	26.16659953867972	26.219838976603526	23.705835240804294
38-39	23.782298942705214	26.24986387566591	26.247438988742793	23.720398192886087
40-41	23.982141554108942	26.09191958673024	26.24145276676065	23.684486092400174
42-43	23.7502006240068	26.08711755773121	26.25487009264861	23.90781172561338
44-45	23.84016388292477	26.152924621041677	26.246488860806878	23.76042263522667
46-47	23.933533211348955	26.2382883975221	25.981702343422764	23.84647604770618
48-49	23.750923755553558	26.219930544164775	26.07594372983832	23.95320197044335
50-51	23.8340348083267	26.29078453210163	26.087286239714857	23.787894419856812
52-53	24.021473560480253	26.153971301422363	26.006666838617402	23.817888299479982
54-55	23.660675312581844	26.09682519666709	26.259167583020805	23.983331907730264
56-57	23.74986358901437	26.244062677403175	26.075387048948116	23.930686684634335
58-59	23.810796288983738	26.179910516346656	26.122585946617153	23.886707248052453
60-61	23.737649202745537	26.17191754215552	26.2249164561628	23.86551679893614
62-63	23.760976207779745	26.17166277420675	26.19534386531853	23.87201715269497
64-65	23.56596748070755	26.022976277430214	26.20904213464314	24.2020141072191
66-67	23.569312255210342	26.23494425038227	26.057972190924243	24.13777130348315
68-69	23.72105589931969	26.078445860181677	26.10890491855345	24.09159332194519
70-71	23.616391153427436	25.911995332066347	26.121258180189738	24.350355334316482
72-73	23.580643603427664	26.05822284761516	26.047174827096274	24.3139587218609
74-75	23.518092086766867	26.177071074645504	25.96234351602375	24.34249332256388
76-77	23.567003530374077	25.925955386217797	26.170748316454794	24.33629276695333
78-79	23.532605697971757	25.957122905952357	26.138833179995984	24.371438216079902
80-81	23.62052721245629	26.118606368224572	25.929709564822616	24.33115685449652
82-83	23.627787533969038	26.1246938266095	25.974881654116906	24.272636985304562
84-85	23.68945576438314	26.079541908059156	25.933311380374274	24.29769094718343
86-87	23.545009494631476	26.107346691703313	25.959522515336126	24.388121298329086
88-89	23.5028184880434	26.00216900315401	25.963984819188934	24.53102768961366
90-91	23.383347491899546	26.073180255720953	25.97321532415304	24.57025692822646
92-93	23.4124653361421	25.98848100123933	26.1211261560409	24.477927506577668
94-95	23.332652829960725	25.89113239435626	26.103131976353755	24.673082799329254
96-97	23.274251448421893	25.824704241487282	26.149378277662223	24.7516660324286
98-99	23.20273706556855	25.826382834260496	26.083004775976605	24.88787532419435
100-101	23.21416525692186	25.801438270372078	26.088435419138406	24.895961053567657
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	13.5
2	22.5
3	28.0
4	25.0
5	33.0
6	50.5
7	120.5
8	190.0
9	207.0
10	204.5
11	203.0
12	195.5
13	198.0
14	248.0
15	289.0
16	316.0
17	359.5
18	412.5
19	474.0
20	577.5
21	675.5
22	824.5
23	1081.0
24	1373.0
25	1820.0
26	2500.5
27	3481.5
28	4915.0
29	6971.5
30	10043.5
31	14255.0
32	19915.5
33	24976.5
34	29265.0
35	34594.5
36	41375.0
37	48271.0
38	54316.5
39	60309.0
40	65781.5
41	71149.5
42	76336.5
43	80641.5
44	85668.5
45	91326.5
46	94723.5
47	97495.0
48	100226.5
49	100308.0
50	98400.0
51	96911.0
52	95988.5
53	95157.0
54	93301.5
55	88225.5
56	80476.5
57	72107.5
58	64458.0
59	56088.0
60	46412.0
61	38046.5
62	30631.0
63	22916.0
64	16361.0
65	11759.5
66	8676.5
67	6503.5
68	4758.0
69	3473.5
70	2588.5
71	1858.5
72	1324.0
73	1000.5
74	710.0
75	457.0
76	295.5
77	177.0
78	100.0
79	62.0
80	39.0
81	17.0
82	14.0
83	12.5
84	9.5
85	6.0
86	6.0
87	5.0
88	3.0
89	4.0
90	3.0
91	2.0
92	2.0
93	2.0
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15946938183459888
2	8.817770629505054E-5
3	0.010052258517635762
4	0.00643697255953869
5	0.023234825608745818
6	0.0024689757762614154
7	0.0
8	0.0
9	4.408885314752527E-5
10-11	0.04816707206367136
12-13	3.086219720326769E-4
14-15	0.004695462860211441
16-17	2.2044426573762635E-5
18-19	0.0018958206853435867
20-21	2.2044426573762635E-4
22-23	0.0
24-25	8.817770629505054E-4
26-27	0.002953953160884193
28-29	0.006172439440653538
30-31	0.017613496832436345
32-33	0.012344878881307076
34-35	0.010404969342815963
36-37	0.004034130062998562
38-39	0.0
40-41	0.003901863503555986
42-43	0.009831814251898136
44-45	0.008222571112013462
46-47	0.004276618755309951
48-49	0.010184525077078337
50-51	0.003042130867179244
52-53	0.0024469313496876524
54-55	0.0044529741679000525
56-57	0.008134393405718412
58-59	0.01580585385338781
60-61	0.007759638153964448
62-63	0.022705759370975517
64-65	0.04139943310552623
66-67	0.06194483867227301
68-69	0.0515619137560308
70-71	0.07140189767241718
72-73	0.03405863905646327
74-75	0.027445311084334484
76-77	0.22987928031119675
78-79	0.08385699868659306
80-81	0.06902109960245081
82-83	0.0282609548675637
84-85	0.05195871343435854
86-87	0.070674431595483
88-89	0.2971368257877466
90-91	0.3021849994731382
92-93	0.052752312791013986
94-95	0.009611369986160509
96-97	0.014725676951273439
98-99	0.001080176902114369
100-101	2.645331188851516E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2268147.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.3745986464301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.33527383930998	56.29641303206735
2	10.592840922517777	14.485624932052666
3	3.114250527363002	6.388068896786356
4	1.3357350446757055	3.653213903106909
5	0.733870404274147	2.5089047175369097
6	0.4482629118339391	1.83898780108354
7	0.2937654654753266	1.4060267058640021
8	0.21347393570061635	1.1676955740002775
9	0.15600978094139464	0.9600395541106804
>10	0.741444911648243	8.699654328031102
>50	0.026333274218693813	1.2095264093189224
>100	0.007899083107631119	0.9873838941972922
>500	8.39898933521011E-4	0.3984602518439257
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.408885314752527E-5	0.0
3	0.0	0.0	0.0	4.408885314752527E-5	0.0
4	0.0	0.0	0.0	4.408885314752527E-5	0.0
5	0.0	0.0	0.0	8.817770629505054E-5	0.0
6	0.0	0.0	0.0	8.817770629505054E-5	4.408885314752527E-5
7	0.0	0.0	0.0	8.817770629505054E-5	4.408885314752527E-5
8	0.0	0.0	0.0	8.817770629505054E-5	4.408885314752527E-5
9	0.0	0.0	0.0	1.322665594425758E-4	1.322665594425758E-4
10-11	0.0	0.0	0.0	1.7635541259010108E-4	1.7635541259010108E-4
12-13	0.0	2.2044426573762635E-5	0.0	1.983998391638637E-4	2.2044426573762635E-4
14-15	0.0	6.61332797212879E-5	0.0	2.2044426573762635E-4	2.645331188851516E-4
16-17	0.0	8.817770629505054E-5	0.0	2.2044426573762635E-4	3.967996783277274E-4
18-19	0.0	8.817770629505054E-5	0.0	2.2044426573762635E-4	3.967996783277274E-4
20-21	0.0	8.817770629505054E-5	0.0	2.42488692311389E-4	4.408885314752527E-4
22-23	0.0	8.817770629505054E-5	0.0	2.645331188851516E-4	4.6293295804901533E-4
24-25	0.0	8.817770629505054E-5	0.0	3.0862197203267687E-4	4.8497738462277795E-4
26-27	0.0	8.817770629505054E-5	0.0	4.84977384622778E-4	4.8497738462277795E-4
28-29	0.0	1.1022213286881318E-4	0.0	0.0013447100209995207	5.070218111965406E-4
30-31	0.0	1.322665594425758E-4	0.0	0.005489062216866896	5.290662377703032E-4
32-33	0.0	1.322665594425758E-4	0.0	0.01615856467856801	5.731550909178285E-4
34-35	0.0	1.322665594425758E-4	0.0	0.029495442755694407	6.613327972128791E-4
36-37	0.0	1.322665594425758E-4	0.0	0.05277435721758775	6.613327972128791E-4
38-39	0.0	1.322665594425758E-4	0.0	0.09571690018327736	6.613327972128791E-4
40-41	0.0	1.322665594425758E-4	0.0	0.14646317015607896	6.613327972128791E-4
42-43	0.0	1.322665594425758E-4	0.0	0.19778259521979835	7.054216503604043E-4
44-45	0.0	1.322665594425758E-4	0.0	0.26179960999000507	7.054216503604043E-4
46-47	0.0	1.322665594425758E-4	0.0	0.3390212362778956	7.054216503604043E-4
48-49	0.0	1.322665594425758E-4	0.0	0.42484018892955355	7.495105035079296E-4
50-51	0.0	1.322665594425758E-4	0.0	0.5199398451687656	7.935993566554548E-4
52-53	0.0	1.322665594425758E-4	0.0	0.6123280369394046	8.156437832292175E-4
54-55	0.0	1.322665594425758E-4	0.0	0.7181633289200391	8.376882098029802E-4
56-57	0.0	1.322665594425758E-4	0.0	0.8432434052995683	8.597326363767427E-4
58-59	0.0	1.322665594425758E-4	0.0	0.9812415156513224	9.038214895242681E-4
60-61	0.0	1.322665594425758E-4	0.0	1.1352659241222018	9.258659160980307E-4
62-63	0.0	1.322665594425758E-4	0.0	1.2940298843064406	9.699547692455559E-4
64-65	0.0	1.322665594425758E-4	0.0	1.457621573910333	0.0010140436223930811
66-67	0.0	2.2044426573762635E-4	0.0	1.6441835560040863	0.0010140436223930811
68-69	0.0	2.2044426573762635E-4	0.0	1.855677784552765	0.0010140436223930811
70-71	0.0	2.2044426573762635E-4	0.0	2.0850941319059126	0.0010581324755406065
72-73	0.0	2.2044426573762635E-4	0.0	2.320771096406009	0.0011242657552618944
74-75	0.0	2.2044426573762635E-4	0.0	2.567360052060118	0.001146310181835657
76-77	0.0	2.2044426573762635E-4	0.0	2.8351337016516127	0.001146310181835657
78-79	0.0	2.2044426573762635E-4	0.0	3.142146430544405	0.001146310181835657
80-81	0.0	2.2044426573762635E-4	0.0	3.469836831563386	0.001146310181835657
82-83	0.0	2.2044426573762635E-4	0.0	3.8190425929183602	0.0011903990349831823
84-85	0.0	2.2044426573762635E-4	0.0	4.180196433476313	0.0011903990349831823
86-87	0.0	2.2044426573762635E-4	0.0	4.558236304789769	0.001212443461556945
88-89	0.0	2.2044426573762635E-4	0.0	4.980916139915093	0.0012344878881307075
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1775	0.0	45.256252	1
GTATCAA	3065	0.0	40.011005	1
TCAACGC	3655	0.0	32.981865	4
CAACGCA	3680	0.0	32.649	5
ATCAACG	3775	0.0	31.933437	3
TATCAAC	4020	0.0	30.223368	2
AACGCAG	4005	0.0	29.994946	6
ACGCAGA	4965	0.0	24.385511	7
CGCAGAG	5005	0.0	23.526564	8
GTACATG	5580	0.0	23.510677	1
TACATGG	5920	0.0	21.565498	2
ACATGGG	6110	0.0	19.341364	3
GTATATA	1020	0.0	19.106184	1
GCAGAGT	6195	0.0	18.240911	9
CATGGGG	5280	0.0	17.977322	4
AGAGTAC	5045	0.0	17.740305	10-11
TCTATAC	700	0.0	17.628048	3
GTATAGG	660	0.0	16.56434	1
GTATAGA	785	0.0	16.348742	1
GTATAGT	640	0.0	16.339281	1
>>END_MODULE