FastQCFastQC Report
Mon 15 Aug 2016
SRR2921695_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921695_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758903
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.044.377861
GTATCAA45950.041.2129481
TCAACGC54000.034.27954
CAACGCA56700.032.3938565
ATCAACG57650.032.0264053
TATCAAC59450.031.0575522
AACGCAG60750.030.532546
GTACATG96400.026.3896771
ACGCAGA70900.025.8304127
TACATGG102250.024.1848742
CGCAGAG76400.023.599238
ACATGGG102000.023.219793
CATGGGG87500.020.2331964
GCAGAGT88750.019.353499
GTATATG12250.018.5973681
GTATAGC11250.018.5629271
AGAGTAC72050.018.17643510-11
GAGTACA71350.017.8273641
GTATAGG9900.017.258921
GTATATA15450.017.2030661
Produced by FastQC (version 0.11.5)