##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921695_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3758903
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.251616495557347	31.0	31.0	34.0	26.0	34.0
2	30.312878251979367	31.0	31.0	34.0	26.0	34.0
3	30.376017949917834	33.0	31.0	34.0	26.0	34.0
4	33.81708120693724	37.0	35.0	37.0	29.0	37.0
5	33.80984398905745	37.0	35.0	37.0	29.0	37.0
6	33.850339846492446	37.0	35.0	37.0	29.0	37.0
7	33.75991745464036	37.0	35.0	37.0	29.0	37.0
8	33.73062566392375	37.0	35.0	37.0	29.0	37.0
9	35.28004340628104	39.0	37.0	39.0	29.0	39.0
10-11	35.17361328557826	39.0	35.0	39.0	28.0	39.0
12-13	35.06640900283939	39.0	35.0	39.0	27.0	39.0
14-15	36.19993293256038	40.0	37.0	41.0	27.0	41.0
16-17	36.14154196051348	40.0	36.0	41.0	27.0	41.0
18-19	36.10414953511703	40.0	36.5	41.0	27.0	41.0
20-21	36.028072951071096	40.0	36.5	41.0	27.0	41.0
22-23	35.92721493478284	40.0	36.0	41.0	27.0	41.0
24-25	35.8206181431125	40.0	36.0	41.0	27.0	41.0
26-27	35.6742898127459	40.0	36.0	41.0	26.5	41.0
28-29	35.5350960107244	40.0	36.0	41.0	25.5	41.0
30-31	35.39631828754294	40.0	36.0	41.0	25.0	41.0
32-33	35.22324984177565	39.0	35.0	41.0	24.5	41.0
34-35	35.05091831313551	39.0	35.0	41.0	24.0	41.0
36-37	35.02365916332505	39.0	35.0	41.0	24.0	41.0
38-39	35.208723130125996	39.5	35.0	41.0	24.0	41.0
40-41	35.27546653904078	40.0	35.0	41.0	24.0	41.0
42-43	35.12254293340371	40.0	35.0	41.0	23.5	41.0
44-45	35.00407459303951	40.0	35.0	41.0	23.0	41.0
46-47	34.85399809465687	40.0	35.0	41.0	22.0	41.0
48-49	34.69281902193273	39.0	35.0	41.0	20.5	41.0
50-51	33.007956842727786	37.0	32.5	39.5	17.0	40.0
52-53	33.124631308655744	37.5	32.5	39.5	15.5	40.0
54-55	34.04115176688518	39.0	34.0	40.5	18.0	41.0
56-57	34.05958546948405	39.0	34.0	41.0	17.5	41.0
58-59	33.876277068070124	38.0	34.0	41.0	14.5	41.0
60-61	33.62860520742355	38.0	34.0	40.0	13.0	41.0
62-63	33.33993787549187	37.0	33.5	40.0	11.0	41.0
64-65	33.04961806676043	37.0	33.0	40.0	8.0	41.0
66-67	32.71152660762994	36.0	33.0	39.5	7.0	41.0
68-69	32.3554138002497	36.0	33.0	39.0	3.5	41.0
70-71	31.95295821679889	35.0	32.0	39.0	2.0	40.5
72-73	31.597534041181696	35.0	32.0	37.5	2.0	40.0
74-75	31.20951538254645	35.0	32.0	37.0	2.0	39.0
76-77	30.841224022008547	35.0	32.0	36.5	2.0	39.0
78-79	30.505086989475387	35.0	31.0	36.0	2.0	38.5
80-81	30.200546941487982	35.0	31.0	36.0	2.0	37.0
82-83	29.902247277995734	35.0	31.0	35.0	2.0	37.0
84-85	29.6506982223271	35.0	31.0	35.0	2.0	36.5
86-87	29.429742400907926	35.0	31.0	35.0	2.0	36.0
88-89	29.22088133160127	35.0	30.5	35.0	2.0	36.0
90-91	29.00985500290909	34.0	30.0	35.0	2.0	35.5
92-93	28.83664076992676	34.0	30.0	35.0	2.0	35.0
94-95	28.623169845031914	34.0	30.0	35.0	2.0	35.0
96-97	28.406169831996195	34.0	30.0	35.0	2.0	35.0
98-99	28.15707415168734	34.0	29.5	35.0	2.0	35.0
100-101	27.105616851512263	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	142640.0
3	23816.0
4	15904.0
5	12014.0
6	10867.0
7	11035.0
8	11536.0
9	12218.0
10	13451.0
11	14459.0
12	14383.0
13	14143.0
14	15025.0
15	15923.0
16	15680.0
17	16637.0
18	15684.0
19	15067.0
20	15261.0
21	15986.0
22	17636.0
23	19868.0
24	23304.0
25	27496.0
26	32947.0
27	38959.0
28	46266.0
29	54692.0
30	65489.0
31	80608.0
32	99849.0
33	129761.0
34	179553.0
35	262992.0
36	442127.0
37	802939.0
38	860532.0
39	152136.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09163006100987	17.777745836830203	12.870232181409676	25.26039192075025
2	15.763523716420993	19.337812308983295	39.57823511746928	25.320428857126426
3	18.961098980554016	23.678519117207802	29.171744729064407	28.188637173173774
4	13.24749304911462	15.712142299611646	36.528673001174546	34.51169165009919
5	14.418751868422586	36.96179779588027	34.37631355265564	14.2431367830415
6	33.70007401861481	35.189173167906254	17.114183956744608	13.996568856734337
7	29.1291096332344	31.40635829922081	21.094616185898502	18.369915881646286
8	26.591264912454353	34.86574134681189	19.86508146837852	18.677912272355243
9	26.749898102416914	15.511924943783395	19.178189341140335	38.559987612659356
10-11	25.65235061737921	25.046073124997093	27.917939084896542	21.38363717272716
12-13	26.27884937383509	22.443322386684354	27.64248616080225	23.635342078678303
14-15	23.43588571221745	23.981852644151825	24.721554070274028	27.860707573356695
16-17	23.786407508635776	27.039117190845026	25.525324827903145	23.64915047261605
18-19	23.811594663688183	26.511491843783453	26.6125291288697	23.064384363658665
20-21	24.415211097159215	25.766992864310133	26.411126109621456	23.406669928909192
22-23	24.214032812992343	25.550413888250173	26.17731406964564	24.058239229111845
24-25	24.01801495160614	25.747364081841965	26.183566805534248	24.051054161017646
26-27	23.91069466402334	25.916181153951	26.12834725045538	24.04477693157028
28-29	24.004946664779382	26.074420272811544	26.01937159903493	23.90126146337414
30-31	24.20076690617851	25.80855867720923	25.942376142683287	24.048298273928967
32-33	23.980345281213943	25.92751241589762	25.863685263763248	24.228457039125193
34-35	24.35706631108349	25.881273565278285	25.900735039806232	23.860925083831997
36-37	24.37425052326196	25.699594758800675	25.915949530078503	24.010205187858862
38-39	24.172056416013227	25.825715452893917	25.909293647857652	24.092934483235208
40-41	24.331049347892915	25.698793528399015	25.98370753209722	23.986449591610846
42-43	24.18444624359541	25.678806580768686	25.888230064274836	24.24851711136106
44-45	24.107562915005268	25.779085720286947	25.968204575690056	24.145146789017726
46-47	24.321777518555137	25.747276940502275	25.77921179553056	24.15173374541203
48-49	24.079247312085943	25.822289366201183	25.807853766172073	24.290609555540804
50-51	24.141288358350117	25.901061949804188	25.891785532974797	24.0658641588709
52-53	24.328801092344364	25.74126985565678	25.891368570787254	24.0385604812116
54-55	23.976391732707988	25.61256552039246	26.120507672913458	24.290535073986096
56-57	24.028711752574132	25.80497553260389	25.977999403660117	24.18831331116186
58-59	24.064122008785525	25.60140210075173	26.063365932246906	24.271109958215842
60-61	24.030997314822677	25.625840409312577	26.17035432529771	24.172807950567034
62-63	24.048461331660302	25.688861627079508	26.126792205166264	24.135884836093922
64-65	23.89821456157372	25.588151832042723	26.117706740675505	24.39592686570805
66-67	23.890164727584374	25.66414179447638	26.036901431353286	24.40879204658596
68-69	24.0595454086853	25.54496859742449	26.072434305689406	24.323051688200806
70-71	23.92421512645627	25.421924044809597	26.099859788441936	24.5540010402922
72-73	23.918140112650978	25.509115239336676	26.049525378457698	24.523219269554648
74-75	23.908037030318678	25.647423480924058	26.08685163522052	24.357687853536746
76-77	23.978687885311846	25.348944696347147	26.160663427397246	24.511703990943765
78-79	23.94778872677337	25.401149924460725	26.188960415928538	24.462100932837362
80-81	24.02311703505575	25.511244172349674	26.06902689504284	24.396611897551736
82-83	23.95054839140565	25.50223331823392	26.162270639297176	24.384947651063253
84-85	23.90228458960101	25.54739971886691	26.16325314575131	24.387062545780772
86-87	23.978007567065106	25.56297679136494	26.092762010762627	24.366253630807325
88-89	23.873359882115622	25.42162556564811	26.13684554765296	24.56816900458331
90-91	23.79398127902487	25.434106476612644	26.216178092448683	24.555734151913803
92-93	23.947577802450727	25.304514509934418	26.317007590665092	24.430900096949763
94-95	23.81254200531455	25.207495088019677	26.401115973132068	24.57884693353371
96-97	23.890818429779934	25.078382737624526	26.419385879333984	24.611412953261556
98-99	23.78923443071903	25.066145387461646	26.358015301066338	24.786604880752982
100-101	23.96111540219991	24.9370969937623	26.490502249388303	24.61128535464949
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	37.0
2	59.0
3	97.0
4	89.0
5	84.5
6	136.5
7	304.0
8	492.5
9	550.5
10	517.0
11	528.0
12	601.5
13	676.5
14	766.0
15	850.0
16	982.0
17	1081.5
18	1189.0
19	1347.5
20	1477.5
21	1600.5
22	1768.5
23	1984.0
24	2289.0
25	2745.5
26	3461.5
27	4527.0
28	6293.0
29	9178.5
30	13369.0
31	19503.5
32	28002.5
33	35367.5
34	41767.5
35	50146.5
36	60363.0
37	71517.0
38	82459.0
39	92701.0
40	102154.0
41	110785.5
42	119984.0
43	130050.0
44	138866.0
45	148836.0
46	156327.5
47	161024.0
48	166020.0
49	168166.0
50	167023.0
51	164774.5
52	163535.0
53	161873.5
54	158303.5
55	150599.0
56	138799.5
57	125260.5
58	113048.5
59	99635.5
60	82961.5
61	68252.5
62	55733.5
63	42797.0
64	31247.0
65	22898.5
66	17178.0
67	13302.5
68	10116.0
69	7532.0
70	5665.5
71	4133.5
72	2914.0
73	2122.5
74	1554.5
75	1148.0
76	831.0
77	568.0
78	379.5
79	281.0
80	209.5
81	163.0
82	143.5
83	124.0
84	98.5
85	72.0
86	66.5
87	60.5
88	60.0
89	58.0
90	40.0
91	36.0
92	36.0
93	28.5
94	21.0
95	20.0
96	15.5
97	10.5
98	11.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052408907598839344
2	0.07148362168430523
3	0.06781233780174695
4	0.06775913078895625
5	0.06363558729767702
6	0.010348763987791118
7	0.013514581248837759
8	0.0168932265610472
9	0.006038995951744432
10-11	0.012649967290988886
12-13	0.02201440154215206
14-15	0.038335652715699234
16-17	0.02633747133139642
18-19	0.010362065740988793
20-21	0.02000583680930314
22-23	0.03636699324244334
24-25	0.03316127072180367
26-27	0.020285173626454312
28-29	0.010934041128488817
30-31	0.011306490218023716
32-33	0.00836680276133755
34-35	0.005187683747093235
36-37	8.114069450581725E-4
38-39	0.003924017193314113
40-41	0.001316873566569821
42-43	0.22263144326948578
44-45	0.1940459756476823
46-47	0.19995195406744998
48-49	0.20644320962791537
50-51	0.19831583842413597
52-53	0.20498001677617111
54-55	0.20192061354070587
56-57	0.20448785190785718
58-59	0.21176391090698535
60-61	0.21619339472181112
62-63	0.2028118310049501
64-65	0.20323748710727574
66-67	0.20106930133605472
68-69	0.19632057544448472
70-71	0.1881532989811123
72-73	0.19934007342035698
74-75	0.19783697530901967
76-77	0.19244976526396132
78-79	0.20947600935698527
80-81	0.2194523242552415
82-83	0.21830837348024143
84-85	0.21310738797995052
86-87	0.23155691966512568
88-89	0.2247597237811138
90-91	0.23067900395407917
92-93	0.22631602890524177
94-95	0.22968137246425352
96-97	0.23609281750553288
98-99	0.2298942005154163
100-101	0.23633224906309103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3758903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.62061189445058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.43347159548779	45.391974860390526
2	12.20119475278797	14.30483004503581
3	4.290025334057337	7.544517303754077
4	1.9249483973532595	4.513666116723602
5	1.0877399402238048	3.188199043897627
6	0.6741082312359468	2.3709982198882193
7	0.4594914058915464	1.8854967161522664
8	0.33816474387756423	1.585873936578638
9	0.24502146356924817	1.2926977309532843
>10	1.2671696088769142	13.0348996833095
>50	0.05951794328292921	2.3395647210258437
>100	0.01764522554825933	1.6864854669983689
>500	8.189224492601148E-4	0.3322530247973137
>1k	6.824353580853337E-4	0.5285431304949256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.660350639534992E-5	0.0
5	0.0	0.0	0.0	2.660350639534992E-5	0.0
6	0.0	0.0	0.0	2.660350639534992E-5	2.660350639534992E-5
7	0.0	0.0	0.0	2.660350639534992E-5	2.660350639534992E-5
8	0.0	0.0	0.0	2.660350639534992E-5	2.660350639534992E-5
9	0.0	0.0	0.0	2.660350639534992E-5	2.660350639534992E-5
10-11	0.0	0.0	0.0	2.660350639534992E-5	2.660350639534992E-5
12-13	1.330175319767496E-5	0.0	0.0	2.660350639534992E-5	7.981051918604977E-5
14-15	2.660350639534992E-5	0.0	0.0	2.660350639534992E-5	1.4631928517442455E-4
16-17	2.660350639534992E-5	0.0	0.0	3.990525959302488E-5	3.059403235465241E-4
18-19	2.660350639534992E-5	0.0	0.0	5.320701279069984E-5	3.724490895348989E-4
20-21	2.660350639534992E-5	0.0	0.0	5.320701279069984E-5	3.990525959302488E-4
22-23	2.660350639534992E-5	0.0	0.0	6.65087659883748E-5	4.3895785552327366E-4
24-25	2.660350639534992E-5	0.0	0.0	1.7292279156977446E-4	4.7886311511629855E-4
26-27	2.660350639534992E-5	0.0	0.0	3.591473363372239E-4	5.320701279069984E-4
28-29	2.660350639534992E-5	0.0	0.0	0.0015563051241279703	5.719753875000233E-4
30-31	2.660350639534992E-5	0.0	0.0	0.0076352063354654266	5.852771406976982E-4
32-33	2.660350639534992E-5	0.0	0.0	0.023238162836338155	5.985788938953732E-4
34-35	2.660350639534992E-5	0.0	2.660350639534992E-5	0.04458747671860647	6.38484153488398E-4
36-37	2.660350639534992E-5	0.0	2.660350639534992E-5	0.07927844905814277	6.38484153488398E-4
38-39	2.660350639534992E-5	0.0	2.660350639534992E-5	0.1400275559119243	6.51785906686073E-4
40-41	2.660350639534992E-5	0.0	2.660350639534992E-5	0.20971544091454342	7.182946726744478E-4
42-43	3.990525959302488E-5	0.0	2.660350639534992E-5	0.28556203764768606	7.315964258721228E-4
44-45	5.320701279069984E-5	0.0	2.660350639534992E-5	0.3738191701142594	7.448981790697977E-4
46-47	6.65087659883748E-5	0.0	2.660350639534992E-5	0.4804593255000196	7.448981790697977E-4
48-49	7.981051918604975E-5	0.0	2.660350639534992E-5	0.596051560787815	7.448981790697977E-4
50-51	7.981051918604975E-5	0.0	2.660350639534992E-5	0.7210082303267735	7.448981790697977E-4
52-53	7.981051918604975E-5	0.0	2.660350639534992E-5	0.8451534929206739	7.581999322674727E-4
54-55	7.981051918604975E-5	0.0	2.660350639534992E-5	0.9857530242200983	7.981051918604976E-4
56-57	7.981051918604975E-5	2.660350639534992E-5	2.660350639534992E-5	1.1511603252331866	8.114069450581725E-4
58-59	7.981051918604975E-5	5.320701279069984E-5	2.660350639534992E-5	1.331319270542496	8.247086982558475E-4
60-61	7.981051918604975E-5	5.320701279069984E-5	2.660350639534992E-5	1.5263761794332016	8.513122046511975E-4
62-63	7.981051918604975E-5	5.320701279069984E-5	2.660350639534992E-5	1.7268735053817563	9.045192174418973E-4
64-65	7.981051918604975E-5	5.320701279069984E-5	2.660350639534992E-5	1.9384378899907766	9.045192174418973E-4
66-67	9.311227238372472E-5	5.320701279069984E-5	2.660350639534992E-5	2.1865155871274147	9.045192174418973E-4
68-69	1.0641402558139968E-4	5.320701279069984E-5	2.660350639534992E-5	2.4599198223524255	9.045192174418973E-4
70-71	1.0641402558139968E-4	5.320701279069984E-5	2.660350639534992E-5	2.752917540037612	9.444244770349221E-4
72-73	1.0641402558139968E-4	5.320701279069984E-5	2.660350639534992E-5	3.054162344705357	0.001050838502616322
74-75	1.0641402558139968E-4	5.320701279069984E-5	2.660350639534992E-5	3.3674053307574043	0.0010907437622093468
76-77	1.0641402558139968E-4	5.320701279069984E-5	2.660350639534992E-5	3.716616257455965	0.0011040455154070219
78-79	1.0641402558139968E-4	5.320701279069984E-5	2.660350639534992E-5	4.111625120414121	0.0011439507750000465
80-81	1.0641402558139968E-4	5.320701279069984E-5	2.660350639534992E-5	4.527690658684196	0.0011971577877907464
82-83	1.330175319767496E-4	5.320701279069984E-5	2.660350639534992E-5	4.964666552981016	0.001276968306976796
84-85	1.330175319767496E-4	5.320701279069984E-5	2.660350639534992E-5	5.423763262845569	0.001276968306976796
86-87	1.330175319767496E-4	6.65087659883748E-5	2.660350639534992E-5	5.903504293672915	0.001316873566569821
88-89	1.330175319767496E-4	7.981051918604975E-5	2.660350639534992E-5	6.423616145455203	0.001356778826162846
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2770	0.0	44.37786	1
GTATCAA	4595	0.0	41.212948	1
TCAACGC	5400	0.0	34.2795	4
CAACGCA	5670	0.0	32.393856	5
ATCAACG	5765	0.0	32.026405	3
TATCAAC	5945	0.0	31.057552	2
AACGCAG	6075	0.0	30.53254	6
GTACATG	9640	0.0	26.389677	1
ACGCAGA	7090	0.0	25.830412	7
TACATGG	10225	0.0	24.184874	2
CGCAGAG	7640	0.0	23.59923	8
ACATGGG	10200	0.0	23.21979	3
CATGGGG	8750	0.0	20.233196	4
GCAGAGT	8875	0.0	19.35349	9
GTATATG	1225	0.0	18.597368	1
GTATAGC	1125	0.0	18.562927	1
AGAGTAC	7205	0.0	18.176435	10-11
GAGTACA	7135	0.0	17.827364	1
GTATAGG	990	0.0	17.25892	1
GTATATA	1545	0.0	17.203066	1
>>END_MODULE