FastQCFastQC Report
Mon 15 Aug 2016
SRR2921695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758903
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.044.3103181
GTATCAA48250.040.8872951
TCAACGC58350.032.862134
CAACGCA61200.031.5879845
ATCAACG61550.031.3074283
TATCAAC65750.029.9572372
AACGCAG66000.029.2152086
GTACATG93900.025.0091531
ACGCAGA78200.023.8067687
TACATGG97150.023.6945442
CGCAGAG81200.022.810238
ACATGGG103000.021.4732693
CATGGGG88950.018.9425134
GCAGAGT96150.018.1277399
AGAGTAC79450.017.96294210-11
CGTATAC3451.0968506E-917.884373
GGACCGT5650.016.8115756
GAGTACA76200.016.1578061
TAAGGTG18450.015.961525
GTATATA13900.015.7318821
Produced by FastQC (version 0.11.5)