##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3758903
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305094598078217	33.0	31.0	34.0	28.0	34.0
2	31.439531161086094	34.0	31.0	34.0	28.0	34.0
3	31.512930501265927	34.0	31.0	34.0	28.0	34.0
4	34.984856486054575	37.0	35.0	37.0	32.0	37.0
5	34.71651755844724	37.0	35.0	37.0	32.0	37.0
6	34.68713079321281	37.0	35.0	37.0	32.0	37.0
7	34.57101234056851	37.0	35.0	37.0	32.0	37.0
8	34.51731289687444	37.0	35.0	37.0	32.0	37.0
9	36.1022529711461	39.0	37.0	39.0	32.0	39.0
10-11	35.98752308851811	39.0	37.0	39.0	31.0	39.0
12-13	35.909636401897046	39.0	37.0	39.0	31.0	39.0
14-15	37.14834660537929	40.0	38.0	41.0	31.0	41.0
16-17	37.115705566225046	40.0	38.0	41.0	31.0	41.0
18-19	37.07683026138211	40.0	38.0	41.0	31.0	41.0
20-21	37.01650109087678	40.0	38.0	41.0	31.0	41.0
22-23	36.9254239867323	40.0	37.5	41.0	30.0	41.0
24-25	36.842677504580465	40.0	37.0	41.0	30.0	41.0
26-27	36.71639132480939	40.0	37.0	41.0	30.0	41.0
28-29	36.5956284320186	40.0	37.0	41.0	30.0	41.0
30-31	36.44698094098199	40.0	37.0	41.0	30.0	41.0
32-33	36.40034220090276	40.0	37.0	41.0	29.5	41.0
34-35	36.52917832676182	40.0	37.0	41.0	30.0	41.0
36-37	36.530156005621855	40.0	37.0	41.0	30.0	41.0
38-39	36.42683344050113	40.0	37.0	41.0	30.0	41.0
40-41	36.34498110219923	40.0	37.0	41.0	29.0	41.0
42-43	36.22396161858925	40.0	36.5	41.0	29.0	41.0
44-45	36.0983809638078	40.0	36.0	41.0	28.0	41.0
46-47	35.948161471578274	40.0	36.0	41.0	28.0	41.0
48-49	35.809203908693576	40.0	36.0	41.0	27.5	41.0
50-51	35.65512198638805	40.0	35.0	41.0	27.0	41.0
52-53	35.48425404965225	39.0	35.0	41.0	26.5	41.0
54-55	35.27012841246502	39.0	35.0	41.0	26.0	41.0
56-57	35.043789903596874	39.0	35.0	41.0	26.0	41.0
58-59	34.80265412541904	38.5	35.0	40.5	26.0	41.0
60-61	34.529222887635036	38.0	34.5	40.0	25.5	41.0
62-63	34.226471925452714	37.5	34.0	40.0	24.5	41.0
64-65	33.889039169140574	37.0	34.0	40.0	23.5	41.0
66-67	33.53654204431452	36.5	34.0	39.0	23.0	41.0
68-69	33.1785091288602	36.0	34.0	39.0	22.0	41.0
70-71	32.78185350885617	35.0	33.0	39.0	22.0	40.0
72-73	32.42302235519246	35.0	33.0	37.5	20.5	40.0
74-75	32.02264530369632	35.0	33.0	37.0	20.0	39.0
76-77	29.338639890414836	33.0	29.0	34.5	13.0	36.5
78-79	30.89962989201903	34.5	31.5	36.0	15.0	37.0
80-81	31.081194566606268	35.0	32.0	35.5	18.0	37.0
82-83	30.91026996440185	35.0	32.0	35.0	16.5	37.0
84-85	30.71396601614886	35.0	32.0	35.0	13.5	36.0
86-87	30.504541085524153	35.0	32.0	35.0	8.5	36.0
88-89	30.240817334206284	35.0	32.0	35.0	2.0	36.0
90-91	30.05021252211084	34.5	31.5	35.0	2.0	35.0
92-93	29.897915562066913	34.0	31.0	35.0	2.0	35.0
94-95	29.797288198179096	34.0	31.0	35.0	2.0	35.0
96-97	29.600300140759153	34.0	31.0	35.0	2.0	35.0
98-99	29.40635964801433	34.0	31.0	35.0	2.0	35.0
100-101	28.30923596591878	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78618.0
3	26617.0
4	14046.0
5	9770.0
6	8581.0
7	8710.0
8	8515.0
9	9384.0
10	10024.0
11	11026.0
12	11467.0
13	12255.0
14	13148.0
15	14186.0
16	15249.0
17	15899.0
18	14988.0
19	13653.0
20	12972.0
21	12939.0
22	13807.0
23	15631.0
24	18726.0
25	22510.0
26	27029.0
27	32753.0
28	39407.0
29	47346.0
30	58032.0
31	72139.0
32	91839.0
33	121420.0
34	171926.0
35	260227.0
36	464689.0
37	906474.0
38	955253.0
39	117644.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.79590019392703	19.38710744964372	13.49991126999023	26.317081086439014
2	14.818891068605748	20.72203076539986	38.01340180020846	26.445676365785932
3	17.554517975240856	24.80482401236015	29.090248291154175	28.55040972124482
4	12.8842451634462	17.002938274942082	35.52452624174309	34.588290319868634
5	14.135295449713317	37.28319154184572	34.46330119109107	14.118211817349893
6	33.64931809916435	35.81022929735335	16.86263628239737	13.677816321084931
7	28.89162077340118	31.642210506629194	21.182642914701443	18.283525805268187
8	26.452983756165033	35.43501920640144	19.640677080520565	18.47131995691296
9	26.571323221515218	15.473907007950727	19.143569052877677	38.81120071765638
10-11	25.538526358038467	25.369609529714417	27.907567242351934	21.184296869895185
12-13	26.127695257209265	22.640380037268937	27.728817608475044	23.50310709704675
14-15	23.28134367778891	24.17358689248239	24.736583129108684	27.80848630062001
16-17	23.66830162949137	27.31940675244879	25.50930417730918	23.502987440750665
18-19	23.703472446877342	26.753010496833074	26.608152284955388	22.935364771334196
20-21	24.241393153363873	25.940761345681988	26.429774479055958	23.38807102189818
22-23	24.08028352952976	25.71065281546238	26.21607953171444	23.992984123293418
24-25	23.917205213036635	25.918663624629012	26.1966716716983	23.96745949063605
26-27	23.780001386078638	26.127475869661254	26.157950976713213	23.9345717675469
28-29	23.865580335360097	26.26265528615433	26.025219849597175	23.8465445288884
30-31	24.09820369127932	25.999852858633467	25.933107086852385	23.96883636323483
32-33	23.883360123901333	26.063805940415453	25.84607132494846	24.206762610734756
34-35	24.15959665162314	26.04777789233179	25.961693876940167	23.830931579104906
36-37	24.21214792947895	25.868530187050848	25.99041865889957	23.92890322457063
38-39	24.080895410176854	26.019572731725187	25.93789996709146	23.961631891006498
40-41	24.201081311748958	25.880142110393617	25.987544323033507	23.931232254823918
42-43	24.096215809650023	25.866449527433495	25.85056583332258	24.186768829593902
44-45	24.022674104445507	25.847797972090504	25.997931703652032	24.13159621981196
46-47	24.222334227111062	25.90873942754328	25.710522699679643	24.158403645666017
48-49	24.001956590509337	25.987640541811146	25.811257392754015	24.199145474925494
50-51	24.032860456791635	26.00030834280111	25.81216336528366	24.154667835123597
52-53	24.23023474493156	25.906836033446094	25.798052023977174	24.064877197645167
54-55	23.88159230214015	25.772043379211063	26.02978802312146	24.316576295527327
56-57	23.926252563446294	25.933416378173646	25.891166739211176	24.249164319168884
58-59	23.981787762295813	25.758444126574627	25.947968684146666	24.31179942698289
60-61	23.93119301447602	25.81238376748975	26.026504338016775	24.229918880017454
62-63	23.938559222250046	25.859425037445554	25.986762540768353	24.215253199536043
64-65	23.76977463361678	25.730321244604436	25.97432166990186	24.525582451876918
66-67	23.781584723803864	25.85428688706563	25.838075600496403	24.526052788634104
68-69	23.88473295954368	25.76518858721618	25.906976682286338	24.443101770953803
70-71	23.763022271868486	25.6017206050656	25.92795458786754	24.70730253519837
72-73	23.738044293994605	25.739098152554014	25.8138129986534	24.709044554797984
74-75	23.680452125217908	25.804073395305938	25.81989341378968	24.695581065686472
76-77	23.698194261288467	25.507692373322865	26.016317931167244	24.77779543422142
78-79	23.61539858362085	25.583861009741266	25.993709951663757	24.80703045497413
80-81	23.669839290577404	25.704110337351622	25.808904626149403	24.817145745921568
82-83	23.61279664533857	25.655027687851817	25.89264865214401	24.8395270146656
84-85	23.500495214611185	25.611285942319807	25.98359523412166	24.90462360894735
86-87	23.48796871894879	25.717286691457275	25.830773144095183	24.96397144549875
88-89	23.359425255554182	25.558219769260184	25.907643239877153	25.174711735308485
90-91	23.208690428369962	25.578061383014216	25.966399525493454	25.24684866312236
92-93	23.281317415985292	25.475440319127728	26.03300087756399	25.210241387322995
94-95	23.128609266061027	25.353267412609366	26.136972852598937	25.381150468730667
96-97	23.07443324363998	25.255743128454135	26.174641020380957	25.49518260752493
98-99	22.9379430190153	25.255076794396608	26.11907361471592	25.687906571872173
100-101	22.858374981177995	25.178203824631577	26.16786888071983	25.795552313470598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	27.5
2	36.0
3	65.0
4	74.0
5	79.0
6	118.5
7	264.5
8	412.5
9	434.5
10	372.5
11	319.5
12	336.5
13	350.0
14	402.0
15	460.5
16	519.5
17	587.0
18	614.0
19	677.0
20	840.0
21	1021.0
22	1212.5
23	1523.5
24	1934.0
25	2541.5
26	3454.5
27	4792.5
28	6874.5
29	10202.0
30	14808.5
31	21214.5
32	29802.0
33	36807.0
34	43163.5
35	51198.5
36	61201.5
37	72223.5
38	82631.5
39	92718.0
40	102196.5
41	111142.5
42	120162.5
43	130200.0
44	138722.0
45	148272.5
46	156342.5
47	161428.5
48	167030.5
49	169407.0
50	168064.0
51	166584.5
52	165025.5
53	162731.0
54	159593.0
55	151538.0
56	139098.0
57	124909.0
58	112423.0
59	98917.0
60	81845.0
61	66968.0
62	54827.5
63	41967.0
64	29940.5
65	21675.0
66	16215.0
67	12513.0
68	9406.5
69	6880.5
70	5039.5
71	3621.0
72	2509.0
73	1774.0
74	1270.0
75	869.5
76	575.5
77	333.5
78	215.5
79	121.0
80	65.0
81	47.0
82	28.0
83	18.5
84	9.5
85	8.0
86	10.5
87	11.5
88	8.5
89	4.0
90	2.5
91	2.0
92	1.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15815784552035528
2	1.330175319767496E-4
3	0.009896504379070171
4	0.006251824002907231
5	0.02447522588372193
6	0.0019154524604651942
7	0.0
8	0.0
9	2.660350639534992E-5
10-11	0.04598416080436234
12-13	2.261298043604743E-4
14-15	0.004642311865988561
16-17	0.0
18-19	0.0014631928517442455
20-21	1.5962103837209953E-4
22-23	0.0
24-25	5.054666215116484E-4
26-27	0.0025938418735466174
28-29	0.005240890759883934
30-31	0.016294647667151826
32-33	0.01139960249040744
34-35	0.009723581587500396
36-37	0.003697887388953639
38-39	0.0
40-41	0.003591473363372239
42-43	0.008819062370058499
44-45	0.0067572906244188794
46-47	0.0036845856357559637
48-49	0.008619536072093373
50-51	0.002647048886337317
52-53	0.002088375252034969
54-55	0.0038309049209303884
56-57	0.007741620361046826
58-59	0.014658532023837807
60-61	0.0072627572459305284
62-63	0.02075073498837294
64-65	0.040769873550873754
66-67	0.060150527959886166
68-69	0.050227420074420645
70-71	0.0678389413081423
72-73	0.034132298705233946
74-75	0.02676312743372202
76-77	0.2209421206133811
78-79	0.0832423715110499
80-81	0.0664023519627934
82-83	0.027335102821222045
84-85	0.05177042344535095
86-87	0.06670829228633993
88-89	0.28923332153024434
90-91	0.292891303659605
92-93	0.05086590422790904
94-95	0.009258020225581772
96-97	0.014419100466279658
98-99	9.311227238372471E-4
100-101	1.330175319767496E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3758903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.646870825928545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3760687228524	44.02841367858513
2	12.8291972663339	14.791261552254115
3	4.419715295037564	7.643482701012331
4	2.0544011302774	4.7371918632697145
5	1.1280091179327076	3.25130979559682
6	0.7188914281289686	2.4865104777130767
7	0.46592541793872555	1.880139966770134
8	0.34411311965996694	1.5869635646836324
9	0.25580690082025787	1.3271820631169866
>10	1.3260404641094952	13.314983198492806
>50	0.06174597860276263	2.3749570953819776
>100	0.01851284719954517	1.7226134403944038
>500	8.78644459872585E-4	0.35026570737041335
>1k	6.93666646444176E-4	0.5047248953585641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	0.0
6	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	0.0
7	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	0.0
8	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	2.660350639534992E-5
9	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	5.320701279069984E-5
10-11	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	5.320701279069984E-5
12-13	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	7.981051918604975E-5
14-15	2.660350639534992E-5	5.320701279069984E-5	0.0	0.0	9.311227238372472E-5
16-17	2.660350639534992E-5	5.320701279069984E-5	0.0	2.660350639534992E-5	1.596210383720995E-4
18-19	2.660350639534992E-5	5.320701279069984E-5	0.0	5.320701279069984E-5	1.8622454476744944E-4
20-21	2.660350639534992E-5	5.320701279069984E-5	0.0	6.65087659883748E-5	2.1282805116279937E-4
22-23	2.660350639534992E-5	6.65087659883748E-5	0.0	9.311227238372472E-5	2.926385703488491E-4
24-25	2.660350639534992E-5	7.981051918604975E-5	0.0	1.9952629796512442E-4	3.19242076744199E-4
26-27	2.660350639534992E-5	7.981051918604975E-5	0.0	4.1235434912792376E-4	3.591473363372239E-4
28-29	2.660350639534992E-5	7.981051918604975E-5	0.0	0.0015297016177326203	3.724490895348989E-4
30-31	2.660350639534992E-5	7.981051918604975E-5	0.0	0.007648508088663102	3.724490895348989E-4
32-33	2.660350639534992E-5	7.981051918604975E-5	0.0	0.02378353471744283	3.990525959302488E-4
34-35	2.660350639534992E-5	7.981051918604975E-5	0.0	0.04565161697442047	3.990525959302488E-4
36-37	2.660350639534992E-5	7.981051918604975E-5	0.0	0.08051551210552654	3.990525959302488E-4
38-39	2.660350639534992E-5	7.981051918604975E-5	0.0	0.14166367155523832	3.990525959302488E-4
40-41	2.660350639534992E-5	7.981051918604975E-5	0.0	0.21155108285582255	4.1235434912792376E-4
42-43	2.660350639534992E-5	7.981051918604975E-5	0.0	0.28779673218489543	4.2565610232559874E-4
44-45	2.660350639534992E-5	7.981051918604975E-5	0.0	0.3761469769238525	4.5225960872094864E-4
46-47	2.660350639534992E-5	7.981051918604975E-5	0.0	0.4828669428287987	4.7886311511629855E-4
48-49	2.660350639534992E-5	7.981051918604975E-5	0.0	0.5983926693506058	4.921648683139735E-4
50-51	2.660350639534992E-5	7.981051918604975E-5	0.0	0.7241740475878202	5.054666215116484E-4
52-53	2.660350639534992E-5	7.981051918604975E-5	0.0	0.8487848715436392	5.586736343023483E-4
54-55	2.660350639534992E-5	1.1971577877907465E-4	0.0	0.989317894077075	5.852771406976982E-4
56-57	2.660350639534992E-5	1.330175319767496E-4	0.0	1.15485821262214	6.118806470930482E-4
58-59	2.660350639534992E-5	1.330175319767496E-4	0.0	1.3339663194288334	6.38484153488398E-4
60-61	2.660350639534992E-5	1.330175319767496E-4	0.0	1.5287305897491903	6.38484153488398E-4
62-63	2.660350639534992E-5	1.330175319767496E-4	0.0	1.7285894315442563	6.916911662790979E-4
64-65	2.660350639534992E-5	1.330175319767496E-4	0.0	1.9405794722556022	7.049929194767729E-4
66-67	2.660350639534992E-5	1.330175319767496E-4	0.0	2.188005383485554	7.315964258721228E-4
68-69	2.660350639534992E-5	1.330175319767496E-4	0.0	2.4610371696210303	7.448981790697977E-4
70-71	2.660350639534992E-5	1.4631928517442455E-4	0.0	2.7541413013317984	7.848034386628226E-4
72-73	2.660350639534992E-5	1.596210383720995E-4	0.0	3.0561842111914035	7.981051918604976E-4
74-75	2.660350639534992E-5	1.596210383720995E-4	0.0	3.3701055866565324	8.114069450581725E-4
76-77	2.660350639534992E-5	1.596210383720995E-4	0.0	3.7202077308193378	8.247086982558475E-4
78-79	2.660350639534992E-5	1.596210383720995E-4	0.0	4.117664116365865	8.247086982558475E-4
80-81	2.660350639534992E-5	1.8622454476744944E-4	0.0	4.535192847487684	8.380104514535225E-4
82-83	2.660350639534992E-5	1.8622454476744944E-4	0.0	4.97522814501997	9.178209706395723E-4
84-85	2.660350639534992E-5	1.8622454476744944E-4	0.0	5.438514907141791	9.311227238372472E-4
86-87	2.660350639534992E-5	1.8622454476744944E-4	0.0	5.9203842184807645	9.710279834302721E-4
88-89	2.660350639534992E-5	1.8622454476744944E-4	0.0	6.442916989345029	0.0010242349962209718
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2725	0.0	44.310318	1
GTATCAA	4825	0.0	40.887295	1
TCAACGC	5835	0.0	32.86213	4
CAACGCA	6120	0.0	31.587984	5
ATCAACG	6155	0.0	31.307428	3
TATCAAC	6575	0.0	29.957237	2
AACGCAG	6600	0.0	29.215208	6
GTACATG	9390	0.0	25.009153	1
ACGCAGA	7820	0.0	23.806768	7
TACATGG	9715	0.0	23.694544	2
CGCAGAG	8120	0.0	22.81023	8
ACATGGG	10300	0.0	21.473269	3
CATGGGG	8895	0.0	18.942513	4
GCAGAGT	9615	0.0	18.127739	9
AGAGTAC	7945	0.0	17.962942	10-11
CGTATAC	345	1.0968506E-9	17.88437	3
GGACCGT	565	0.0	16.811575	6
GAGTACA	7620	0.0	16.157806	1
TAAGGTG	1845	0.0	15.96152	5
GTATATA	1390	0.0	15.731882	1
>>END_MODULE